LOCUS       sequence-377690-        8517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the puromycin resistance gene fused to a thymidine kinase,
            upon excision of this cassette the reading frame of eGFP is 
            restored.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8517)
  AUTHORS   Thomsen EA, Skipper KA, Andersen S, Haslund D, Skov TW, Mikkelsen JG
  TITLE     CRISPR-Cas9-directed gene tagging using a single integrase-defective
            lentiviral vector carrying a transposase-based Cas9 off switch.
  JOURNAL   Mol Ther Nucleic Acids. 2022 Aug 4;29:563-576. doi: 
            10.1016/j.omtn.2022.08.005. eCollection 2022 Sep 13.
  PUBMED    36090759
REFERENCE   2  (bases 1 to 8517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Nucleic Acids."; date: "2022-08-4"; volume: "29:563-576. doi"; 
            pages: " 10.1016/j.omtn.2022.08.005. eCollection 2022 Sep 13"
COMMENT     Sequence Label: pT2/CMV-eGFP.PB/PTK
FEATURES             Location/Qualifiers
     source          1..8517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        1105..1408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1409..1612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1563..1583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1609..1633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(1695..1716)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     repeat_region   1955..1989
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    2279..2303
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2399..2898
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2434..2457)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2831..2850
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        2910..2957
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2976..3575
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     3475..3495
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3582..4577
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    4605..4829
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(4914..4938)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     repeat_region   5120..5182
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5188..5207)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    5643..5850
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     6233..6249
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6283..6299)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6284..6300)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(6329..6349)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6329..6347)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6368..6384)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6368..6384)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6381..6403)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6392..6408
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6416..6422,6423..6440,6441..6446))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6461..6482
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6599..6616)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6770..7358)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6850..6869)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7529..8320,8321..8389))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8152..8171
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8390..8494)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcgt
      661 ttaaacggcg cgttcggatc cctatacagt tgaagtcgga agtttacata cacttaagtt
      721 ggagtcatta aaactcgttt ttcaactact ccacaaattt cttgttaaca aacaatagtt
      781 ttggcaagtc agttaggaca tctactttgt gcatgacaca agtcattttt ccaacaattg
      841 tttacagaca gattatttca cttataattc actgtatcac aattccagtg ggtcagaagt
      901 ttacatacac taagttgact gtgcctttaa acagcttgga aaattccaga aaatgatgtc
      961 atggctttag aagcttggta ccgcggccgc tctagagttt aaaccctagg atcgatgaat
     1021 tcgtttaaac gtcgacggcg cgcctagtta ttaatagtaa tcaattacgg ggtcattagt
     1081 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     1141 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     1201 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     1261 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     1321 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     1381 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     1441 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     1501 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     1561 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
     1621 gaaccgtcag atcggcgcgc cgcggccgca tggtgagcaa gggcgaggag ctgttcaccg
     1681 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     1741 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     1801 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     1861 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1921 aaggctacgt ccaggagcgc accatcttct ttaaccctag aaagatagtc tgcgtaaaat
     1981 tgacgcatgc attcttgaaa tattgctctc tctttctaaa tagcgcgaat ccgtcgctgt
     2041 gcatttagga catctcagtc gccgcttgga gctcccgtga ggcgtgcttg tcaatgcggt
     2101 aagtgtcact gattttgaac tataacgacc gcgtgagtca aaatgacgca tgattatctt
     2161 ttacgtgact tttaagattt aactcatacg ataattatat tgttatttca tgttctactt
     2221 acgtgataac ttattatata tatattttct tgttatagat atcaactaga atgctagcac
     2281 aagtttgtac aaaaaagcag gctggcgccg gaaccaattc agtcgactgg atccggtacc
     2341 gggccccccc tcgaggtcga gacggtatcg ataagcttga tatcgaatta attctaccgg
     2401 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
     2461 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
     2521 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
     2581 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
     2641 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
     2701 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
     2761 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
     2821 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
     2881 tcatctccgg gcctttcgac ctgcagcctg ttgacaatta atcatcggca tagtatatcg
     2941 gcatagtata atacgacaag gtgaggaact aaaccatggg gaccgagtac aagcccacgg
     3001 tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg
     3061 ccgactaccc cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg
     3121 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     3181 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     3241 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     3301 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     3361 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     3421 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     3481 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     3541 cgcgcacctg gtgcatgacc cgcaagcccg gtgccggatc catgcccacg ctactgcggg
     3601 tttatataga cggtcctcac gggatgggga aaaccaccac cacgcaactg ctggtggccc
     3661 tgggttcgcg cgacgatatc gtctacgtac ccgagccgat gacttactgg caggtgctgg
     3721 gggcttccga gacaatcgcg aacatctaca ccacacaaca ccgcctcgac cagggtgaga
     3781 tatcggccgg ggacgcggcg gtggtaatga caagcgccca gataacaatg ggcatgcctt
     3841 atgccgtgac cgacgccgtt ctggctcctc atatcggggg ggaggctggg agctcacatg
     3901 ccccgccccc ggccctcacc ctcatcttcg accgccatcc catcgccgcc ctcctgtgct
     3961 acccggccgc gcgatacctt atgggcagca tgacccccca ggccgtgctg gcgttcgtgg
     4021 ccctcatccc gccgaccttg cccggcacaa acatcgtgtt gggggccctt ccggaggaca
     4081 gacacatcga ccgcctggcc aaacgccagc gccccggcga gcggcttgac ctggctatgc
     4141 tggccgcgat tcgccgcgtt tacgggctgc ttgccaatac ggtgcggtat ctgcagggcg
     4201 gcgggtcgtg gcgggaggat tggggacagc tttcggggac ggccgtgccg ccccagggtg
     4261 ccgagcccca gagcaacgcg ggcccacgac cccatatcgg ggacacgtta tttaccctgt
     4321 ttcgggcccc cgagttgctg gcccccaacg gcgacctgta caacgtgttt gcctgggcct
     4381 tggacgtctt ggccaaacgc ctccgtccca tgcacgtctt tatcctggat tacgaccaat
     4441 cgcccgccgg ctgccgggac gccctgctgc aacttacctc cgggatggtc cagacccacg
     4501 tcaccacccc cggctccata ccgacgatct gcgacctggc gcgcacgttt gcccgggaga
     4561 tgggggaggc taactgagct ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
     4621 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     4681 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     4741 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     4801 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     4861 gagatccact agttctagcc tcgaggctag agcggcccac tcgagatatc tagacccagc
     4921 tttcttgtac aaagtggtac tagttaaaag ttttgttact ttatagaaga aattttgagt
     4981 ttttgttttt ttttaataaa taaataaaca taaataaatt gtttgttgaa tttattatta
     5041 gtatgtaagt gtaaatataa taaaacttaa tatctattca aattaataaa taaacctcga
     5101 tatacagacc gataaaacac atgcgtcaat tttacgcatg attatcttta acgtacgtca
     5161 caatatgatt atctttctag ggttaaggac gacggcaact acaagacccg cgccgaggtg
     5221 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     5281 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     5341 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     5401 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     5461 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     5521 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     5581 atggacgagc tgtacaagta agcggccgct taattaagtt taaacccgct gatcagcctc
     5641 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     5701 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     5761 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     5821 ttgggaagac aatagcaggc atgctgggga ttaattaaga attcaccggt actagtagct
     5881 tgtggaaggc tactcgaaat gtttgaccca agttaaacaa tttaaaggca atgctaccaa
     5941 atactaattg agtgtatgta aacttctgac ccactgggaa tgtgatgaaa gaaataaaag
     6001 ctgaaatgaa tcattctctc tactattatt ctgatatttc acattcttaa aataaagtgg
     6061 tgatcctaac tgacctaaga cagggaattt ttactaggat taaatgtcag gaattgtgaa
     6121 aaagtgagtt taaatgtatt tggctaaggt gtatgtaaac ttccgacttc aactgtatag
     6181 ggatcctctg gcccagcagg ccccagaaga ccattaatta aggccgggcc gctctagaac
     6241 tagtggatcc cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga
     6301 gggggggccc ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
     6361 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6421 tacgagccgg gagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6481 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6541 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6601 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6661 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6721 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6781 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6841 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6901 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6961 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7021 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7081 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7141 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7201 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7261 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7321 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7381 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7441 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7501 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7561 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7621 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7681 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7741 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7801 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7861 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7921 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7981 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8041 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8101 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8161 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8221 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8281 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8341 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8401 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8461 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
