LOCUS       sequence-377721-        9232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet on shRNA for conditional Yap1 knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Shao DD, Xue W, Krall EB, Bhutkar A, Piccioni F, Wang X, Schinzel 
            AC, Sood S, Rosenbluh J, Kim JW, Zwang Y, Roberts TM, Root DE, Jacks
            T, Hahn WC
  TITLE     KRAS and YAP1 Converge to Regulate EMT and Tumor Survival.
  JOURNAL   Cell. 2014 Jul 3;158(1):171-84. doi: 10.1016/j.cell.2014.06.004. 
            Epub 2014 Jun 19.
  PUBMED    24954536
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.06.004"; journalName: "Cell"; date: "2014-07-3-
            3"; volume: "158"; issue: "1"; pages: "171-84"
COMMENT     Sequence Label: TRMPVneo.Yap.3093
FEATURES             Location/Qualifiers
     source          1..9232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             681..856
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    919..1118
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1319..1735
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1665..1687
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1703..1719
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    join(1785..1803,1804..1826,1827..1845,1846..1868,1869..1887,
                     1888..1910,1911..1929,1930..1952,1953..1971,1972..1994,
                     1995..2013,2014..2036,2037..2055)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2088..2126
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2123..2147
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2225..2902
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(2405..2425)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     2817..2840
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     ncRNA           2971..3092
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           3120..3134
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           3162..3288
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        3311..3810
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3346..3369)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3743..3762
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(3833..3835,3836..3838,3839..4552)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3878..3899)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4139..4158)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4486..4507
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4584..5157
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4751..4768)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4978..4997
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5173..5967
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5173..5967
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5227..5246)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5837..5856
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             6106..6531
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6634..6656)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6792..6812)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6797..7126
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6977..7112
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7148..7167
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7248..7265)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7419..8007)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7499..7518)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8178..8969,8970..9038))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8801..8820
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9039..9143)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9211..9229
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttcgtcttca agaattagct tggccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc
      721 ttccgataga ctgcgtcgcc cgggtacccg tattcccaat aaagcctctt gctgtttgca
      781 tccgaatcgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccacg
      841 acgggggtct ttcatttggg ggctcgtccg ggatttggag acccctgccc agggaccacc
      901 gacccaccac cgggaggtaa gctggccagc aacttatctg tgtctgtccg attgtctagt
      961 gtctatgttt gatgttatgc gcctgcgtct gtactagtta gctaactagc tctgtatctg
     1021 gcggacccgt ggtggaactg acgagttctg aacacccggc cgcaaccctg ggagacgtcc
     1081 cagggacttt gggggccgtt tttgtggccc gacctgagga agggagtcga tgtggaatcc
     1141 gaccccgtca ggatatgtgg ttctggtagg agacgagaac ctaaaacagt tcccgcctcc
     1201 gtctgaattt ttgctttcgg tttggaaccg aagccgcgcg tcttgtctgc tgcagcgctg
     1261 cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg aaaattaggg
     1321 ccagactgtt accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat
     1381 cgctcacaac cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga
     1441 atggccaacc tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac
     1501 ccaggttaag atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta
     1561 catcgtgacc tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca
     1621 ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg
     1681 ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag gcgccggaat
     1741 tgaagatctg ggggatcgat cctctagagc atcgagttta ccactcccta tcagtgatag
     1801 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     1861 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     1921 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     1981 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     2041 tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc gggtcgaggt aggcgtgtac
     2101 ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
     2161 atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc tcgacggatc
     2221 caccatggcc tcctccgaga acgtcatcac cgagttcatg cgcttcaagg tgcgcatgga
     2281 gggcaccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     2341 gggccacaac accgtgaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga
     2401 catcctgtcc ccccagttcc agtacggctc caaggtgtac gtgaagcacc ccgccgacat
     2461 ccccgactac aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     2521 cgaggacggc ggcgtggcga ccgtgaccca ggactcctcc ctgcaggacg gctgcttcat
     2581 ctacaaggtg aagttcatcg gcgtgaactt cccctccgac ggccccgtga tgcagaagaa
     2641 gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg
     2701 cgagacccac aaggccctga agctgaagga cggcggccac tacctggtgg agttcaagtc
     2761 catctacatg gccaagaagc ccgtgcagct gcccggctac tactacgtgg acgccaagct
     2821 ggacatcacc tcccacaacg aggactacac catcgtggag cagtacgagc gcaccgaggg
     2881 ccgccaccac ctgttcctgt agcggccgcg actctagaat ttactagtca tatgataaga
     2941 tctctcgact agggataaca gggtaattgt ttgaatgagg cttcagtact ttacagaatc
     3001 gttgcctgca catcttggaa acacttgctg ggattacttc ttcaggttaa cccaacagaa
     3061 ggctcgagaa ggtatattgc tgttgacagt gagcgaatga attaactctg tgtataatag
     3121 tgaagccaca gatgtattat acacagagtt aattcatgtg cctactgcct cggaattcaa
     3181 ggggctactt taggagcaat tatcttgttt actaaaactg aataccttgc tatctctttg
     3241 atacattttt acaaagctga attaaaatgg tataaattaa atcacttttt tcaattctag
     3301 caattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     3361 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     3421 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     3481 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     3541 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
     3601 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
     3661 ctgggaaggg gtgggtccgg gggcgggctc aggggnnngc tcaggggcgg ggcgggcgcc
     3721 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
     3781 ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttcca ccatggtgag
     3841 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     3901 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     3961 gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     4021 caccctgggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     4081 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     4141 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     4201 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     4261 gtacaactac aacagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa
     4321 ggccaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta
     4381 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     4441 ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     4501 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aagaattgtt
     4561 caattccgcc cctctccccc ccccccctct ccctcccccc cccctaacgt tactggccga
     4621 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     4681 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     4741 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     4801 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     4861 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     4921 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     4981 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     5041 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     5101 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct
     5161 taccggtcca ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     5221 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     5281 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     5341 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     5401 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     5461 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     5521 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     5581 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     5641 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     5701 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     5761 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     5821 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     5881 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     5941 tatcgccttc ttgacgagtt cttctgatgt acgatgcatc gataccgtcg agatatcagt
     6001 ggtccaggct ctagttttga ctcaacaata tcaccagctg aagcctatag agtacgagcc
     6061 atagataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
     6121 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata
     6181 actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagggt cgaccctaga
     6241 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga
     6301 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
     6361 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt
     6421 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
     6481 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat ttgggggctc
     6541 gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg
     6601 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     6661 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     6721 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     6781 gatccggctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     6841 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     6901 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     6961 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7021 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     7081 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttactggctt
     7141 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     7201 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     7261 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7321 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7381 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7441 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7501 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7561 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7621 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7681 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7741 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7801 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     7861 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7921 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7981 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8041 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8101 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8161 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8221 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8281 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8341 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8401 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8461 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
     8521 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8581 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8641 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8701 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8761 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
     8821 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8881 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8941 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9001 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9061 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9121 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     9181 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ct
//
