LOCUS       sequence-377783-        3245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tra synthase (3OC8-HSL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Kokarakis EJ, Rillema R, Ducat DC, Sakkos JK
  TITLE     Developing Cyanobacterial Quorum Sensing Toolkits: Toward 
            Interspecies Coordination in Mixed Autotroph/Heterotroph 
            Communities.
  JOURNAL   ACS Synth Biol. 2023 Jan 20;12(1):265-276. doi: 
            10.1021/acssynbio.2c00527. Epub 2022 Dec 27.
  PUBMED    36573789
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00527"; journalName: "ACS Synth Biol"; date: 
            "2023-01-20- 20"; volume: "12"; issue: "1"; pages: "265-276"
COMMENT     Sequence Label: pBbA2C_Ptet::traI
FEATURES             Location/Qualifiers
     source          1..3245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     266..285
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(352..454)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(484..1110)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     1015..1034
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        1126..1181
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    1162..1180
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      1887..1958
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1974..2001
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(2029..2046)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2163..2708)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2243..2262)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2822..2916)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2937..3245,1..351))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
       61 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
      121 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
      181 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
      241 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
      301 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
      361 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
      421 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc agatatcgac
      481 gtcttaagac ccactttcac atttaagttg tttttctaat ccgcatatga tcaattcaag
      541 gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa
      601 taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc
      661 ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg catataatgc
      721 attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga gagtttcata
      781 ctgtttttct gtaggccgtg tacctaaatg tacttttgct ccatcgcgat gacttagtaa
      841 agcacatcta aaacttttag cgttattacg taaaaaatct tgccagcttt ccccttctaa
      901 agggcaaaag tgagtatggt gcctatctaa catctcaatg gctaaggcgt cgagcaaagc
      961 ccgcttattt tttacatgcc aatacaatgt aggctgctct acacctagct tctgggcgag
     1021 tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg cgctgttaat
     1081 cactttactt ttatctaatc tagacatcat taattcctaa tttttgttga cactctatcg
     1141 ttgatagagt tattttacca ctccctatca gtgatagaga aaagaattca aaagatcttt
     1201 taagaaggag atatacatat gcggatactc acagtttcgc cggaccaata cgaacggtat
     1261 cgaagcttcc tcaaacagat gcaccgcctt cgcgcgacgg tgttcggtgg ccgtctcgaa
     1321 tgggacgtct ccatcatcgc tggggaagag cgcgaccaat acgataattt caagccgagc
     1381 taccttctgg cgattactga cagtgggcgg gtcgccggat gcgtcagact tcttccggct
     1441 tgcgggccta cgatgctgga gcagactttt tcgcaactcc tcgaaatggg ctcgctcgca
     1501 gcgcattccg gaatggtaga gagctctcgc ttctgcgtcg acacctccct cgtctcacgg
     1561 agagatgcga gccagctgca cctggcgact cttaccttat tcgccggtat cattgaatgg
     1621 tcgatggcca gcggctacac ggagatcgtc acggcgaccg atcttcgttt cgagcgcatc
     1681 ctgaagcgtg ctggatggcc gatgcgccgg cttggtgaac ccaccgcgat cggcaacacc
     1741 attgccattg ccggaagact gccagccgat cgtgccagct tcgagcaggt ttgccctccg
     1801 ggctactatt ctatcccccg gatcgacgtg gcagcgatca ggagtgccgc gtgaggatcc
     1861 aaactcgagt aaggatctcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1921 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     1981 cttcgggtgg gcctttctgc gtttatacct agggatatat tccgcttcct cgctcactga
     2041 ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga
     2101 tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg
     2161 tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg
     2221 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg
     2281 cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg
     2341 tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat
     2401 gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2461 caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg
     2521 agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact
     2581 gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa
     2641 aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac
     2701 gatctcaaga agatcatctt attaatcaga taaaatattt ctagatttca gtgcaattta
     2761 tctcttcaaa tgtagcacct gaagtcagcc ccatacgata taagttgtta ctagtgcttg
     2821 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
     2881 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac
     2941 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
     3001 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
     3061 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
     3121 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
     3181 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
     3241 atgtg
//
