LOCUS       sequence-377810-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To fuse your protein of interest to the C-terminus of 
            GPI-anchored-BioID2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Gao SS, Shi R, Sun J, Tang Y, Zheng Z, Li JF, Li H, Zhang J, Leng Q,
            Xu J, Chen X, Zhao J, Sy MS, Feng L, Li C
  TITLE     GPI-anchored ligand-BioID2-tagging system identifies Galectin-1 
            mediating Zika virus entry.
  JOURNAL   iScience. 2022 Nov 2;25(12):105481. doi: 10.1016/j.isci.2022.105481.
            eCollection 2022 Dec 22.
  PUBMED    36404916
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-11-2"; volume: "25(12):105481. doi"; pages: " 
            10.1016/j.isci.2022.105481. eCollection 2022 Dec 22"
COMMENT     Sequence Label: pcDNA3.1-MCS-PrP (1-22)-BioID2-HA-13 x
            Linker-...
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..762
                     /codon_start=1
                     /product="promiscuous R40G mutant of a biotin protein 
                     ligase from Aquifex aeolicus (Kim et al., 2016)"
                     /label=BioID2
                     /translation="FKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGGLGRKWL
                     SQEGGLYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQEKKVS
                     GVLCELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRKEVLLKVLKRISEN
                     LKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKITGKLVGLSEKGGALILTEEGIKEI
                     LSGEFSLRRS"
     CDS             763..789
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atggcgaacc ttggctgctg gatgctggtt ctctttgtgg ccacatggag tgacctgggc
       61 ctctgcttca agaacctgat ctggctgaag gaggtggaca gcacccagga gagactgaag
      121 gagtggaacg ttagctacgg caccgccctg gtggccgaca gacagaccaa gggcagaggc
      181 ggcctgggca gaaagtggct gagccaggag ggcggcctgt acttcagctt cctgctgaac
      241 cccaaggagt tcgagaacct gctgcagctg cccctggtgc tgggcctgag cgtgagcgag
      301 gccctggagg agatcaccga gatccccttc agcctgaagt ggcccaacga cgtgtacttc
      361 caggagaaga aggtgagcgg cgtgctgtgc gagctgagca aggacaagct gatcgtgggc
      421 atcggcatca acgtgaacca gagagagatc cccgaggaga tcaaggacag agccaccacc
      481 ctgtacgaga tcaccggcaa ggactgggac agaaaggagg tgctgctgaa ggtgctgaag
      541 agaatcagcg agaacctgaa gaagttcaag gagaagagct tcaaggagtt caagggcaag
      601 atcgagagca agatgctgta cctgggcgag gaggtgaagc tgctgggcga gggcaagatc
      661 accggcaagc tggtgggcct gagcgagaag ggcggcgccc tgatcctgac cgaggagggc
      721 atcaaggaga tcctgagcgg cgagttcagc ctgagaagaa gctaccctta cgatgtaccg
      781 gattacgca
//
