LOCUS       sequence-377944-        9970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Barcoded piggybac transposon vector with Dox-inducible expression of
            ZFPM2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Pierson Smela MD, Kramme CC, Fortuna PRJ, Adams JL, Su R, Dong E, 
            Kobayashi M, Brixi G, Kavirayuni VS, Tysinger E, Kohman RE, Shioda 
            T, Chatterjee P, Church GM
  TITLE     Directed differentiation of human iPSCs to functional ovarian 
            granulosa-like cells via transcription factor overexpression.
  JOURNAL   Elife. 2023 Feb 21;12:e83291. doi: 10.7554/eLife.83291.
  PUBMED    36803359
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-02-21"; volume: "12:e83291. doi"; pages: " 
            10.7554/eLife.83291"
COMMENT     Sequence Label: pPB-cT3G-cERP2-ZFPM2
FEATURES             Location/Qualifiers
     source          1..9970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    21..39
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    57..75
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    93..111
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    129..147
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    165..183
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    201..219
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    237..255
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     282..306
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    372..396
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3853..3877)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3879..3905
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4072..4206
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   4478..4512
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(4523..4540)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4523..4539)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4532..4554)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4748..4767)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4930..4948)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5016..5120
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5121..5189,5190..5981)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5339..5358)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6152..6740
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6641..6660
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6894..6911
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7117..7138
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7153..7158,7159..7176,7177..7183)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7191..7207
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7196..7218
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7215..7231
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7215..7231
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   7257..7319
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    7495..7576
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(8293..8349)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(8359..9102)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(9103..9126)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      9126..9135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9155..9366)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        9367..9746
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    join(9955..9970,1..3)
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 agaacgtatg aagagtttac tccctatcag tgatagagaa cgtatgcaga stttactccc
       61 tatcagtgat agagaacgta trmrgasttt actccctatc agtgatagag aacgtataag
      121 gagtttactc cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag
      181 agaacgtatc tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc
      241 tatcagtgat agagaacgta taagcttgcc tatataagca gagctcgttt agtgaaccgt
      301 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
      361 tccagcctac cacaagtttg tacaaaaaag ttggcaccat gtcccggcga aagcaaagca
      421 aaccccggca gatcaaacgg ccgcttgaag atgccattga agatgaggaa gaagaatgtc
      481 catcagagga aacagacatc atctccaaag gagactttcc attggaggaa agcttttcca
      541 cagaatttgg gcctgaaaat ctgagctgcg aagaagtgga atacttttgt aacaaaggtg
      601 atgatgaagg aatccaggag acagcagaat cagatgggga cacacagtca gagaaaccgg
      661 ggcaacctgg agttgagaca gacgactggg atggaccagg agagctggag gtgtttcaga
      721 aagatgggga acgaaaaatt cagagtcgac agcaacttcc agtgggaaca acctgggggc
      781 cgtttcctgg gaagatggac ttgaataata attctttgaa gacaaaggct caggtcccaa
      841 tggtgctgac tgctggtccc aagtggttgc tggatgtgac ttggcaagga gtggaagaca
      901 acaaaaacaa ctgcattgtg tacagcaaag ggggtcagct ttggtgtaca actacgaagg
      961 ccatctctga gggtgaagag ctaattgcct ttgtggtgga ttttgactca aggctacaag
     1021 ctgccagtca gatgactctc acagaaggga tgtaccctgc acgcctgctg gactcaattc
     1081 agctgcttcc tcagcaagct gccatggctt ctattttgcc cacagctatt gtcaataagg
     1141 atatattccc ttgcaagtcc tgtggcatct ggtatcggag tgagcggaat ctgcaggccc
     1201 atttgatgta ctactgcagt gggaggcaaa gagaagctgc tccggtgtca gaggaaaatg
     1261 aagacagtgc ccatcagatt tccagcctgt gccccttccc acagtgcacc aagagctttt
     1321 caaatgctcg agctctagaa atgcacctga attcacacag tggagtgaaa atggaagaat
     1381 tcctgccccc tggtgctagt ctaaaatgca ccgtctgtag ctacactgct gattccgtga
     1441 tcaactttca ccaacacctg ttctcccatc tcactcaagc tgccttccga tgtaatcact
     1501 gccatttcgg cttccagact cagagggagt tattgcagca ccaggagctc catgtcccta
     1561 gcggcaaact tcccagagaa agtgacatgg aacactctcc aagtgcaact gaagacagct
     1621 tacagccagc cacagactta ttgaccagaa gcgaacttcc ccagagccaa aaggccatgc
     1681 agactaaaga tgcgagctct gacacagagc tggacaagtg tgagaaaaag actcagctct
     1741 ttctcacgaa ccagagacca gagatacagc ctacaacaaa taaacaaagc ttttcttaca
     1801 caaaaataaa gtctgagccc tctagcccaa gacttgcctc atctccagtt cagcctaata
     1861 ttgggccttc tttccctgtg ggccctttcc tatctcagtt ttctttcccc caagatatca
     1921 ccatggtccc tcaagcttca gagatcttag ctaagatgtc tgaactggtg catcggcgac
     1981 tgaggcatgg cagtagtagc taccctcccg tcatttacag ccctttgatg cccaaggggg
     2041 ctacttgttt tgagtgtaac ataacattca ataatttgga taattatcta gtgcacaaaa
     2101 agcattattg cagcagccga tggcagcaga tggctaagtc cccagagttc cctagtgtgt
     2161 cagaaaagat gcctgaagct ttgagtccca acactggcca aacctccata aaccttctca
     2221 acccagctgc tcattctgct gatcctgaga atccacttct tcaaacatct tgcatcaatt
     2281 cttccactgt cttagattta attgggccaa atgggaaggg ccatgacaag gacttttcca
     2341 ctcaaactaa gaagctctcc acctccagta acaatgatga caaaattaat ggaaaacctg
     2401 ttgatgtgaa aaatcccagt gtccccttag tggatgggga aagtgaccca aataagacta
     2461 cctgtgaagc ttgcaacatt accttcagcc ggcacgaaac atacatggtc cacaaacagt
     2521 attactgtgc tacacgccac gaccctccac tgaagaggtc tgcttccaac aaagtgcctg
     2581 ccatgcagag aaccatgcgc acacgcaagc gcagaaagat gtatgagatg tgcctacctg
     2641 agcaggaaca aaggcctcca ctggttcagc agagatttct tgacgtagcc aacctcaata
     2701 atccttgtac ctccactcaa gaacccacag aagggctagg agagtgctac cacccaagat
     2761 gtgatatctt tccaggaatt gtctctaaac acttggaaac ttctctgacg atcaacaagt
     2821 gtgttccagt ttccaaatgt gatactactc attccagtgt ttcctgccta gagatggacg
     2881 tgcccataga tctcagcaaa aagtgtttat ctcagtctga gcggacgacc acgtctccca
     2941 aaaggctgct ggactatcac gagtgcactg tgtgcaagat cagtttcaat aaggtagaaa
     3001 actatctggc ccacaagcag aatttctgcc cggttactgc acatcagcgt aatgacctgg
     3061 gtcaactgga cggcaaagtg tttccgaatc cagaaagcga acgaaacagc cctgatgtca
     3121 gctacgaaag aagcataata aaatgtgaga aaaatgggaa tttgaagcag ccttccccca
     3181 atggaaactt attttcatcc cacctagcaa ccctgcaagg cttgaaggtc tttagtgaag
     3241 ctgctcagct cattgctaca aaagaagaaa acagacattt gtttcttcca caatgccttt
     3301 accctggagc aataaagaaa gcaaaaggag ccgaccagct ttctccatat tatggaatca
     3361 agccaagtga ttatatttct ggttctcttg tcatccataa cactgacatc gagcaaagca
     3421 gaaatgcaga aaatgaatct cctaaaggcc aggcttcctc aaatgggtgt gctgcgctga
     3481 agaaagattc tctgccattg ttgcccaaaa atcgaggaat ggtaatagtg aatggtggac
     3541 tgaaacaaga tgagagacct gctgccaacc cacagcaaga gaacatttcc cagaatcctc
     3601 agcacgaaga cgaccacaaa tctccctcgt ggatctctga gaacccatta gctgccaatg
     3661 agaatgtctc accaggaatt ccctcagcag aggaacagtt gtctagtata gcaaaaggtg
     3721 tgaatggttc cagccaggct ccaaccagtg ggaaatattg ccggctatgt gatatccagt
     3781 tcaacaacct ttcaaacttt ataactcaca agaagtttta ttgctcatca catgcagcag
     3841 aacatgtcaa attgccaact ttcttgtaca aagtggtata cccatacgat gttccagatt
     3901 acgcttagta atgagaaata ctcagaagat gtcactcacc atagtccgca gtgaaagata
     3961 aatgatctca tcaccctttt gagagaagtt ttagagctag aaatagcaag taggccactg
     4021 agcaagaact atgaatgaga tctttagaca tattgcggcc gctttctccg cgatccagac
     4081 atgataagat acattgatga gtttggacaa accaaaacta gaatgcagtg aaaaaaatgc
     4141 cttatttgtg aaatttgtga tgctattgcc ttatttgtaa ccattataag ctgcaataaa
     4201 caagttatct ataacaagaa aatatatata taataagtta tcacgtaagt agaacatgaa
     4261 ataacaatat aattatcgta tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg
     4321 tcattttgac tcacgcggtc gttatagttc aaaatcagtg acacttaccg cattgacaag
     4381 cacgcctcac gggagctcca agcggcgact gagatgtcct aaatgcacag cgacggattc
     4441 gcgctattta gaaagagaga gcaatatttc aagaatgcat gcgtcaattt tacgcagact
     4501 atctttctag ggttaagaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     4561 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     4621 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     4681 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     4741 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     4801 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     4861 ccatgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacacgcgag
     4921 ttcgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     4981 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5041 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5101 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5161 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5221 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5281 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5341 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5401 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5461 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5521 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5581 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5641 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5701 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5761 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5821 gagccggtga gcgtggttca cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5881 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5941 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6001 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6061 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6121 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6181 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6241 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     6301 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6361 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6421 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6481 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6541 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6601 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6661 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6721 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6781 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6841 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     6901 cagtgagcga ggaagcggat gagcgcccaa tacgcaaacc gcctctcacc gcgcgttggc
     6961 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     7021 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     7081 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     7141 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     7201 ataacaattt cacacaggaa acagctatga ccatgattac gccaaggtcg acttaaccct
     7261 agaaagataa tcatattgtg acgtacgtta aagataatca tgcgtaaaat tgacgcatgt
     7321 gttttatcgg tctgtatatc gaggtttatt tattaatttg aatagatatt aagttttatt
     7381 atatttacac ttacatacta ataataaatt caacaaacaa tttatttatg tttatttatt
     7441 tattaaaaaa aaacaaaaac tcaaaatttc ttctataaag taacaaaact tttagcagtg
     7501 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     7561 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     7621 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
     7681 tcctctggag attcatgcgc caggttttct ggtcatgcac cacgttcgtg ggccctcagg
     7741 aacctccacg tcagcggtaa ctgtgaagcc cagtcgctcg taaaaaggaa gattccgggg
     7801 agcactggtt tcgaggaacg ccggcacgcc agcccgttcc gcggcctcaa caccaggaag
     7861 aacgacagcg cttcccaaac ctttaccttg gtgatctggg ctgacaccga cagtggcaag
     7921 aaaccacgca ggctcctttg gtctatgagg agccagcaag ccctccattt gctgttgtgc
     7981 cgccaaccta ctccctgaca gctcggccat ccgcggacct atttccgcga acactgcgcc
     8041 ggcttcgacg ctttccggag tagtccagac cgcaactgcg gcaccatcgt ccgcaaccca
     8101 aactttacca atgtccagac ccacgcgcgt aagaaacagc tcttgcaact cagttaccct
     8161 ctctatgtgt ctgtccggat ctacggtatg gcgggtcgct ggataatctg caaatgctgc
     8221 ggcaagtgtt cggacagccc gtggtacatc gtcacgggta gcgagcctaa cagtgggctt
     8281 atattccgtc atggggccgg ggttctcctc cacgtcgccg gcctgcttca gcaggctgaa
     8341 gttggtggcg ccgctgcccc cggggagcat gtcaaggtca aaatcgtcaa gagcgtcagc
     8401 aggcagcata tcaaggtcaa agtcgtcaag ggcatcggct gggagcatgt ctaagtcaaa
     8461 atcgtcaagg gcgtcggtcg gcccgccgct ttcgcacttt agctgtttct ccaggccaca
     8521 tatgattagt tccaggccga aaaggaaggc aggttcggct ccctgccggt cgaacagctc
     8581 aattgcttgt ttcagaagtg ggggcataga atcggtggta ggtgtctctc tttcctcttt
     8641 tgctacttga tgctcctgtt cctccaatac gcagcccagt gtaaagtggc ccacggcgga
     8701 cagagcgtac agtgcgttct ccagggagaa gccttgctga cacaggaacg cgagctgatt
     8761 ttccagggtt tcgtactgtt tctctgttgg gcgggtgccg agatgcactt tagccccgtc
     8821 acgatgtgag aggagagcac agcggtatga cttggcgttg ttccgcagaa agtcttgcca
     8881 tgactcgcct tccagggggc aggagtgggt atgatgcctg tccagcatct cgattggcag
     8941 ggcatcgagc agggcccgct tgttcttcac gtgccagtac agggtaggct gctcaactcc
     9001 cagcttttga gcgagtttcc ttgtcgtcag gccttcgata ccgactccat tgagtaattc
     9061 cagagcagag tttatgactt tgctcttgtc cagtctagac atcttatcgt catcgtcttt
     9121 gtaatccatg gtggcggatc ccgcgtcacg acacctgtgt tctggcggca aacccgttgc
     9181 gaaaaagaac gttcacggcg actactgcac ttatatacgg ttctccccca ccctcgggaa
     9241 aaaggcggag ccagtacacg acatcacttt cccagtttac cccgcgccac cttctctagg
     9301 caccggttca attgccgacc cctcccccca acttctcggg cactgtgggc catgtgcgct
     9361 ctgccccatg gtaatagcca tgactaatac gtagatgtac tgccaagtag gaaagtgcca
     9421 taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg
     9481 gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata
     9541 ctccacccat tgacgtcaat ggaaagtgcc tattggcgtt actatgggaa catacgtcat
     9601 tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag
     9661 ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta
     9721 ttaataacta gtcaataatc aatgtcataa gctctaatcg cataatcgag ctaagagcga
     9781 ttaatatgct tagctagctt agctaagcta gatttctcta ttctcccaca atatatagca
     9841 gctattagct tagcagtccg aatacgatcg tccacgatcc ataagcgtaa cacatttgag
     9901 tagcgactga cgggcaccgg agccactcga gtggaattat cacctcgagt ttactcccta
     9961 tcagtgatag
//
