LOCUS       sequence-377962-       12290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test if the TCTP promoter can drive the Flp recomhinase in order 
            to remove the OCS terminator and increase circuit output (despite 
            the presence of the lacZ endlinker)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12290)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 12290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP736_L2pV1_1I_Act2_FRT_OCS_TCTP_Flp_lacZ
FEATURES             Location/Qualifiers
     source          1..12290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    845..878
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      879..1586
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1587..1620
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          1687..1875
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2903..3077
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3465..5117
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3531..3549)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5137..5337
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    5680..5735
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5743..5763
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6447..6635
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    7259..7433
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    7503..7524
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7539..7544,7545..7562,7563..7569)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7577..7593
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7582..7604
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7601..7617
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7601..7617
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7613..7936
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    7630..7686
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(7687..7704)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7687..7703)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7696..7718)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7912..7931)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    8068..8092
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8180..8890
                     /label=oriV
                     /note="incP origin of replication"
     promoter        8911..9015
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9016..9810
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9981..10569
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10470..10489
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             10905..12053
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    12199..12223
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaagaagtt cctattctct agaaagtata ggaacttcct gctttaatga gatatgcgag
      901 aagcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga
      961 gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg
     1021 ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt accctactac
     1081 ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt atagcgacat
     1141 ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat ccaattttaa
     1201 aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta ttcaaatttc
     1261 aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat aacgctcact
     1321 gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag ttgagtattg
     1381 gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac ggcggccggg
     1441 taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg ggccccaaat
     1501 gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca gggcgaattt
     1561 tgcgacaaca tgtcgaggct cagcaggaag ttcctattct ctagaaagta taggaacttc
     1621 caacaaacaa cattacaatt actatttaca attacaatga cttcaaaggt gtatgatcct
     1681 gagcaggtaa gtttctgctt ctacctttga tatatatata ataattatca ttaattagta
     1741 gtaatataat atttcaaata tttttttcaa aataaaagaa tgtagtatat agcaattgct
     1801 tttctgtagt ttataagtgt gtatatttta atttataact tttctaatat atgaccaaaa
     1861 tttgttgatg tgcagagaaa aagaatgata actggtccac aatggtgggc aagatgtaaa
     1921 cagatgaacg tgcttgattc tttcattaat tactacgatt cagaaaagca cgctgagaat
     1981 gcagttatct ttcttcacgg aaacgctgca tcttcatatt tgtggagaca tgttgtgcct
     2041 cacattgaac cagtggctag gtgtattatc ccagatctta tcggaatggg aaagtctggt
     2101 aaatctggaa atggttctta cagacttttg gatcattata agtacctcac agcttggttc
     2161 gaacttctca acttacctaa gaagattatt ttcgttggac acgattgggg tgcttgcttg
     2221 gcattccatt actcatacga acaccaagat aagatcaaag ctatagttca tgcagagagt
     2281 gttgtggatg tgatagaatc ttgggatgag tggccagata ttgaagagga tattgctttg
     2341 atcaagtctg aagagggaga aaagatggtt ctcgaaaata actttttcgt ggaaactatg
     2401 cttccttcaa agattatgag aaaattggaa ccagaagagt ttgctgcata tctcgaacct
     2461 ttcaaggaaa aaggagaggt tagaaggcca acactcagtt ggcctaggga aataccatta
     2521 gttaagggag gtaaacctga tgttgtgcag atcgtgagaa attataacgc ttaccttagg
     2581 gcatcagatg atttgcctaa gatgttcatc gagtcagatc caggattttt cagtaatgct
     2641 atagttgaag gtgcaaagaa atttccaaac accgagttcg ttaaggtgaa aggattgcat
     2701 ttctcacaag aagatgctcc tgatgagatg ggaaagtata ttaagtcttt tgttgaaaga
     2761 gtgctcaaaa acgagcaggg ttctcgatct gatgcttctg aagtaacgtc aacaagctcc
     2821 cagtctgagg tatgcacagt ggaaacgcca ggttgtgttg gttcttaagc ttctctagct
     2881 agagtcgatc gacaagctcg agtttctcca taataatgtg tgagtagttc ccagataagg
     2941 gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     3001 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     3061 gtactaaaat ccagatcgct gcaagaattc aagcttggag ccaacactcg aatccccacc
     3121 cgttggacca aacccggctc attaagcgtc ggttcagatt tatttccttt atttaaaaaa
     3181 aaggaaaggg taaaaaatag aaaattggaa acagttaaag cccaaaattg taatttaccg
     3241 agaattgtaa atttacctga aaaccctacg ctatagtttc gactataaat accaaactta
     3301 ggacctcact tcagaatccc ctcgtcgctg cgtctctctc ccgcaacctt cgattttcgt
     3361 ttattcgcat ccatcggaga gagaaaacaa tcaataagcg acctactaca aacaagtaag
     3421 taagagaaaa accaaaagaa gaagagaaac aacaagaaga agtaatggag gacgccaaaa
     3481 acataaagaa aggcccggcg ccattctatc ctctagagga tggaaccgct ggagagcaac
     3541 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
     3601 atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag
     3661 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
     3721 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
     3781 acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag
     3841 tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa
     3901 tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
     3961 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct
     4021 ttgatcgtga caaaacaatt gcactgataa tgaattcctc tggatctact gggttaccta
     4081 agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta
     4141 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg
     4201 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt
     4261 atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt caaagtgcgt
     4321 tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac aaatacgatt
     4381 tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag
     4441 cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc actgagacta
     4501 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg
     4561 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc
     4621 agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta aacaatccgg
     4681 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact
     4741 gggacgagga cgaacacttc ttcatagttg accgcttgaa gtctttaatt aaatacaaag
     4801 gatatcaggt ggcccccgct gaattggaat cgatattgtt acaacacccc aacatcttcg
     4861 acgcgggcgt ggcaggtctg cccgacgatg acgccggtga acttcccgcc gccgttgttg
     4921 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag
     4981 taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc
     5041 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg
     5101 gaaagtccaa attgtaagct tgatcccccg tcgacagcta gctatatcat caatttatgt
     5161 attacacata atatcgcact cagtctttca tctacggcaa tgtaccagct gatataatca
     5221 gttattgaaa tatttctgaa tttaaacttg catcaataaa tttatgtttt tgcttggact
     5281 ataatacctg acttgttatt ttatcaataa atatttaaac tatatttctt tcaagatcgc
     5341 tactagaatt cgagctcgga gccaacactc gaatccccac ccgttggacc aaacccggct
     5401 cattaagcgt cggttcagat ttatttcctt tatttaaaaa aaaggaaagg gtaaaaaata
     5461 gaaaattgga aacagttaaa gcccaaaatt gtaatttacc gagaattgta aatttacctg
     5521 aaaaccctac gctatagttt cgactataaa taccaaactt aggacctcac ttcagaatcc
     5581 cctcgtcgct gcgtctctct cccgcaacct tcgattttcg tttattcgca tccatcggag
     5641 agagaaaaca atcaataagc gacctactgt atttttacaa caattaccaa caacaacaaa
     5701 caacaaacaa cattacaatt actatttaca attacaatgg ctcctaagaa gaagaggaag
     5761 gtgatgagcc agttcgacat cctgtgcaag acccccccca aggtgctggt gcggcagttc
     5821 gtggagagat tcgagaggcc cagcggcgag aagatcgcca gctgtgccgc cgagctgacc
     5881 tacctgtgct ggatgatcac ccacaacggc accgccatca agagggccac cttcatgagc
     5941 tacaacacca tcatcagcaa cagcctgagc ttcgacatcg tgaacaagag cctgcagttc
     6001 aagtacaaga cccagaaggc caccatcctg gaggccagcc tgaagaagct gatccccgcc
     6061 tgggagttca ccatcatccc ttacaacggc cagaagcacc agagcgacat caccgacatc
     6121 gtgtccagcc tgcagctgca gttcgagagc agcgaggagg ccgacaaggg caacagccac
     6181 agcaagaaga tgctgaaggc cctgctgtcc gagggcgaga gcatctggga gatcaccgag
     6241 aagatcctga acagcttcga gtacaccagc aggttcacca agaccaagac cctgtaccag
     6301 ttcctgttcc tggccacatt catcaactgc ggcaggttca gcgacatcaa gaacgtggac
     6361 cccaagagct tcaagctggt gcagaacaag tacctgggcg tgatcattca gtgcctggtg
     6421 accgagacta agacaagcgt gtccaggtaa gtttctgctt ctacctttga tatatatata
     6481 ataattatca ttaattagta gtaatataat atttcaaata tttttttcaa aataaaagaa
     6541 tgtagtatat agcaattgct tttctgtagt ttataagtgt gtatatttta atttataact
     6601 tttctaatat atgaccaaaa tttgttgatg tgcaggcaca tctacttttt cagcgccaga
     6661 ggcaggatcg accccctggt gtacctggac gagttcctga ggaacagcga gcccgtgctg
     6721 aagagagtga acaggaccgg caacagcagc agcaacaagc aggagtacca gctgctgaag
     6781 gacaacctgg tgcgcagcta caacaaggcc ctgaagaaga acgcccccta ccccatcttc
     6841 gctatcaaga acggccctaa gagccacatc ggcaggcacc tgatgaccag ctttctgagc
     6901 atgaagggcc tgaccgagct gacaaacgtg gtgggcaact ggagcgacaa gagggcctcc
     6961 gccgtggcca ggaccaccta cacccaccag atcaccgcca tccccgacca ctacttcgcc
     7021 ctggtgtcca ggtactacgc ctacgacccc atcagcaagg agatgatcgc cctgaaggac
     7081 gagactaacc ccatcgagga gtggcagcac atcgagcagc tgaagggcag cgccgagggc
     7141 agcatcagat accccgcctg gaacggcatc atcagccagg aggtgctgga ctacctgagc
     7201 agctacatca acaggcggat ctgagcttct ctagctagag tcgatcgaca agctcgagtt
     7261 tctccataat aatgtgtgag tagttcccag ataagggaat tagggttcct atagggtttc
     7321 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct
     7381 atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag atcgctttac
     7441 tgagaccgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     7501 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     7561 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     7621 gattacgcca agcttgcatg cctgcaggtc gactctagag gatccccggg taccgagctc
     7681 gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     7741 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     7801 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
     7861 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     7921 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     7981 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgacggtct cagggacacg
     8041 aagtgatccg tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga
     8101 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg
     8161 tccatttgta tgtgccagcc gcctttgcga cgctcaccgg gctggttgcc ctcgccgctg
     8221 ggctggcggc cgtctatggc cctgcaaacg cgccagaaac gccgtcgaag ccgtgtgcga
     8281 gacaccgcgg ccgccggcgt tgtggatacc tcgcggaaaa cttggccctc actgacagat
     8341 gaggggcgga cgttgacact tgaggggccg actcacccgg cgcggcgttg acagatgagg
     8401 ggcaggctcg atttcggccg gcgacgtgga gctggccagc ctcgcaaatc ggcgaaaacg
     8461 cctgatttta cgcgagtttc ccacagatga tgtggacaag cctggggata agtgccctgc
     8521 ggtattgaca cttgaggggc gcgactactg acagatgagg ggcgcgatcc ttgacacttg
     8581 aggggcagag tgctgacaga tgaggggcgc acctattgac atttgagggg ctgtccacag
     8641 gcagaaaatc cagcatttgc aagggtttcc gcccgttttt cggccaccgc taacctgtct
     8701 tttaacctgc ttttaaacca atatttataa accttgtttt taaccagggc tgcgccctgt
     8761 gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc tcgaaccctc ccggcccgct
     8821 aacgcgggcc tcccatcccc ccaggggctg cgcccctcgg ccgcgaacgg cctcacccca
     8881 aaaatggcag cgctggccaa ttcccgagtg cgcggaaccc ctatttgttt atttttctaa
     8941 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     9001 tgaaaaagga agagtatggc taaaatgaga atatcaccgg aattgaaaaa actgatcgaa
     9061 aaataccgct gcgtaaaaga tacggaagga atgtctcctg ctaaggtata taagctggtg
     9121 ggagaaaatg aaaacctata tttaaaaatg acggacagcc ggtataaagg gaccacctat
     9181 gatgtggaac gggaaaagga catgatgcta tggctggaag gaaagctgcc tgttccaaag
     9241 gtcctgcact ttgaacggca tgatggctgg agcaatctgc tcatgagtga ggccgatggc
     9301 gtcctttgct cggaagagta tgaagatgaa caaagccctg aaaagattat cgagctgtat
     9361 gcggagtgca tcaggctctt tcactccatc gacatatcgg attgtcccta tacgaatagc
     9421 ttagacagcc gcttagccga attggattac ttactgaata acgatctggc cgatgtggat
     9481 tgcgaaaact gggaagagga cactccattt aaagatccgc gcgagctgta tgatttttta
     9541 aagacggaaa agcccgaaga ggaacttgtc ttttcccacg gcgacctggg agacagcaac
     9601 atctttgtga aagatggcaa agtaagtggc tttattgatc ttgggagaag cggcagggcg
     9661 gacaagtggt atgacattgc cttctgcgtc cggtcgctca gggaggatat cggggaagaa
     9721 cagtatgtcg agctattttt tgacttactg gggatcaagc ctgattggga gaaaataaaa
     9781 tattatattt tactggatga attgttttag ctgtcagacc aagtttactc atatatactt
     9841 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     9901 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     9961 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    10021 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    10081 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
    10141 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    10201 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    10261 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    10321 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    10381 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    10441 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    10501 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    10561 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggcagatcc tagatgtggc
    10621 gcaacgatgc cggcgacaag caggagcgca ccgacttctt ccgcatcaag tgttttggct
    10681 ctcaggccga ggcccacggc aagtatttgg gcaaggggtc gctggtattc gtgcagggca
    10741 agattcggaa taccaagtac gagaaggacg gccagacggt ctacgggacc gacttcattg
    10801 ccgataaggt ggattatctg gacaccaagg caccaggcgg gtcaaatcag gaataagggc
    10861 acattgcccc ggcgtgagtc ggggcaatcc cgcaaggagg gtgaatgaat cggacgtttg
    10921 accggaaggc atacaggcaa gaactgatcg acgcggggtt ttccgccgag gatgccgaaa
    10981 ccatcgcaag ccgcaccgtc atgcgtgcgc cccgcgaaac cttccagtcc gtcggctcga
    11041 tggtccagca agctacggcc aagatcgagc gcgacagcgt gcaactggct ccccctgccc
    11101 tgcccgcgcc atcggccgcc gtggagcgtt cgcgtcgtct tgaacaggag gcggcaggtt
    11161 tggcgaagtc gatgaccatc gacacgcgag gaactatgac gaccaagaag cgaaaaaccg
    11221 ccggcgagga cctggcaaaa caggtcagcg aggccaagca ggccgcgttg ctgaaacaca
    11281 cgaagcagca gatcaaggaa atgcagcttt ccttgttcga tattgcgccg tggccggaca
    11341 cgatgcgagc gatgccaaac gacacggccc gctctgccct gttcaccacg cgcaacaaga
    11401 aaatcccgcg cgaggcgctg caaaacaagg tcattttcca cgtcaacaag gacgtgaaga
    11461 tcacctacac cggcgtcgag ctgcgggccg acgatgacga actggtgtgg cagcaggtgt
    11521 tggagtacgc gaagcgcacc cctatcggcg agccgatcac cttcacgttc tacgagcttt
    11581 gccaggacct gggctggtcg atcaatggcc ggtattacac gaaggccgag gaatgcctgt
    11641 cgcgcctaca ggcgacggcg atgggcttca cgtccgaccg cgttgggcac ctggaatcgg
    11701 tgtcgctgct gcaccgcttc cgcgtcctgg accgtggcaa gaaaacgtcc cgttgccagg
    11761 tcctgatcga cgaggaaatc gtcgtgctgt ttgctggcga ccactacacg aaattcatat
    11821 gggagaagta ccgcaagctg tcgccgacgg cccgacggat gttcgactat ttcagctcgc
    11881 accgggagcc gtacccgctc aagctggaaa ccttccgcct catgtgcgga tcggattcca
    11941 cccgcgtgaa gaagtggcgc gagcaggtcg gcgaagcctg cgaagagttg cgaggcagcg
    12001 gcctggtgga acacgcctgg gtcaatgatg acctggtgca ttgcaaacgc tagggccttg
    12061 tggggtcagt tccggctggg ggttcagcag cccctgctcg gatctgttgg accggacagt
    12121 agtcatggtt gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
    12181 gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca
    12241 acttaataac acattgcgga cgtttttaat gtactggggt tgaacactct
//
