LOCUS       sequence-377966-       13656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as the 2-input state of the split-AND gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13656)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 13656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP691_L2_2I_FRT-OCS_Rluc_Flp_C1
FEATURES             Location/Qualifiers
     source          1..13656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    845..878
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      879..1586
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1587..1620
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          1687..1875
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2903..3077
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3465..5117
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3531..3549)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5137..5337
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    5680..5735
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     intron          6420..6608
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             7483..7503
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7541..7715
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    8057..8112
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             8567..8587
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8625..8799
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    8869..8890
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8905..8910,8911..8928,8929..8935)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8943..8959
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8948..8970
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8967..8983
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8967..8983
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             8979..9302
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    8996..9052
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(9053..9070)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9053..9069)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9062..9084)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9278..9297)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    9434..9458
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      9546..10256
                     /label=oriV
                     /note="incP origin of replication"
     promoter        10277..10381
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10382..11176
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11347..11935
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11836..11855
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12271..13419
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    13565..13589
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaagaagtt cctattctct agaaagtata ggaacttcct gctttaatga gatatgcgag
      901 aagcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga
      961 gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg
     1021 ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt accctactac
     1081 ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt atagcgacat
     1141 ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat ccaattttaa
     1201 aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta ttcaaatttc
     1261 aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat aacgctcact
     1321 gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag ttgagtattg
     1381 gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac ggcggccggg
     1441 taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg ggccccaaat
     1501 gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca gggcgaattt
     1561 tgcgacaaca tgtcgaggct cagcaggaag ttcctattct ctagaaagta taggaacttc
     1621 caacaaacaa cattacaatt actatttaca attacaatga cttcaaaggt gtatgatcct
     1681 gagcaggtaa gtttctgctt ctacctttga tatatatata ataattatca ttaattagta
     1741 gtaatataat atttcaaata tttttttcaa aataaaagaa tgtagtatat agcaattgct
     1801 tttctgtagt ttataagtgt gtatatttta atttataact tttctaatat atgaccaaaa
     1861 tttgttgatg tgcagagaaa aagaatgata actggtccac aatggtgggc aagatgtaaa
     1921 cagatgaacg tgcttgattc tttcattaat tactacgatt cagaaaagca cgctgagaat
     1981 gcagttatct ttcttcacgg aaacgctgca tcttcatatt tgtggagaca tgttgtgcct
     2041 cacattgaac cagtggctag gtgtattatc ccagatctta tcggaatggg aaagtctggt
     2101 aaatctggaa atggttctta cagacttttg gatcattata agtacctcac agcttggttc
     2161 gaacttctca acttacctaa gaagattatt ttcgttggac acgattgggg tgcttgcttg
     2221 gcattccatt actcatacga acaccaagat aagatcaaag ctatagttca tgcagagagt
     2281 gttgtggatg tgatagaatc ttgggatgag tggccagata ttgaagagga tattgctttg
     2341 atcaagtctg aagagggaga aaagatggtt ctcgaaaata actttttcgt ggaaactatg
     2401 cttccttcaa agattatgag aaaattggaa ccagaagagt ttgctgcata tctcgaacct
     2461 ttcaaggaaa aaggagaggt tagaaggcca acactcagtt ggcctaggga aataccatta
     2521 gttaagggag gtaaacctga tgttgtgcag atcgtgagaa attataacgc ttaccttagg
     2581 gcatcagatg atttgcctaa gatgttcatc gagtcagatc caggattttt cagtaatgct
     2641 atagttgaag gtgcaaagaa atttccaaac accgagttcg ttaaggtgaa aggattgcat
     2701 ttctcacaag aagatgctcc tgatgagatg ggaaagtata ttaagtcttt tgttgaaaga
     2761 gtgctcaaaa acgagcaggg ttctcgatct gatgcttctg aagtaacgtc aacaagctcc
     2821 cagtctgagg tatgcacagt ggaaacgcca ggttgtgttg gttcttaagc ttctctagct
     2881 agagtcgatc gacaagctcg agtttctcca taataatgtg tgagtagttc ccagataagg
     2941 gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     3001 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     3061 gtactaaaat ccagatcgct gcaagaattc aagcttggag ccaacactcg aatccccacc
     3121 cgttggacca aacccggctc attaagcgtc ggttcagatt tatttccttt atttaaaaaa
     3181 aaggaaaggg taaaaaatag aaaattggaa acagttaaag cccaaaattg taatttaccg
     3241 agaattgtaa atttacctga aaaccctacg ctatagtttc gactataaat accaaactta
     3301 ggacctcact tcagaatccc ctcgtcgctg cgtctctctc ccgcaacctt cgattttcgt
     3361 ttattcgcat ccatcggaga gagaaaacaa tcaataagcg acctactaca aacaagtaag
     3421 taagagaaaa accaaaagaa gaagagaaac aacaagaaga agtaatggag gacgccaaaa
     3481 acataaagaa aggcccggcg ccattctatc ctctagagga tggaaccgct ggagagcaac
     3541 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
     3601 atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag
     3661 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
     3721 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
     3781 acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag
     3841 tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa
     3901 tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
     3961 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct
     4021 ttgatcgtga caaaacaatt gcactgataa tgaattcctc tggatctact gggttaccta
     4081 agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta
     4141 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg
     4201 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt
     4261 atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt caaagtgcgt
     4321 tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac aaatacgatt
     4381 tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag
     4441 cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc actgagacta
     4501 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg
     4561 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc
     4621 agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta aacaatccgg
     4681 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact
     4741 gggacgagga cgaacacttc ttcatagttg accgcttgaa gtctttaatt aaatacaaag
     4801 gatatcaggt ggcccccgct gaattggaat cgatattgtt acaacacccc aacatcttcg
     4861 acgcgggcgt ggcaggtctg cccgacgatg acgccggtga acttcccgcc gccgttgttg
     4921 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag
     4981 taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc
     5041 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg
     5101 gaaagtccaa attgtaagct tgatcccccg tcgacagcta gctatatcat caatttatgt
     5161 attacacata atatcgcact cagtctttca tctacggcaa tgtaccagct gatataatca
     5221 gttattgaaa tatttctgaa tttaaacttg catcaataaa tttatgtttt tgcttggact
     5281 ataatacctg acttgttatt ttatcaataa atatttaaac tatatttctt tcaagatcgc
     5341 tactagaatt cgagctcgga gccaacactc gaatccccac ccgttggacc aaacccggct
     5401 cattaagcgt cggttcagat ttatttcctt tatttaaaaa aaaggaaagg gtaaaaaata
     5461 gaaaattgga aacagttaaa gcccaaaatt gtaatttacc gagaattgta aatttacctg
     5521 aaaaccctac gctatagttt cgactataaa taccaaactt aggacctcac ttcagaatcc
     5581 cctcgtcgct gcgtctctct cccgcaacct tcgattttcg tttattcgca tccatcggag
     5641 agagaaaaca atcaataagc gacctactgt atttttacaa caattaccaa caacaacaaa
     5701 caacaaacaa cattacaatt actatttaca attacaatga gccagttcga catcctgtgc
     5761 aagacccccc ccaaggtgct ggtgcggcag ttcgtggaga gattcgagag gcccagcggc
     5821 gagaagatcg ccagctgtgc cgccgagctg acctacctgt gctggatgat cacccacaac
     5881 ggcaccgcca tcaagagggc caccttcatg agctacaaca ccatcatcag caacagcctg
     5941 agcttcgaca tcgtgaacaa gagcctgcag ttcaagtaca agacccagaa ggccaccatc
     6001 ctggaggcca gcctgaagaa gctgatcccc gcctgggagt tcaccatcat cccttacaac
     6061 ggccagaagc accagagcga catcaccgac atcgtgtcca gcctgcagct gcagttcgag
     6121 agcagcgagg aggccgacaa gggcaacagc cacagcaaga agatgctgaa ggccctgctg
     6181 tccgagggcg agagcatctg ggagatcacc gagaagatcc tgaacagctt cgagtacacc
     6241 agcaggttca ccaagaccaa gaccctgtac cagttcctgt tcctggccac attcatcaac
     6301 tgcggcaggt tcagcgacat caagaacgtg gaccccaaga gcttcaagct ggtgcagaac
     6361 aagtacctgg gcgtgatcat tcagtgcctg gtgaccgaga ctaagacaag cgtgtccagg
     6421 taagtttctg cttctacctt tgatatatat ataataatta tcattaatta gtagtaatat
     6481 aatatttcaa atattttttt caaaataaaa gaatgtagta tatagcaatt gcttttctgt
     6541 agtttataag tgtgtatatt ttaatttata acttttctaa tatatgacca aaatttgttg
     6601 atgtgcaggc acatctactt tttcagcgcc agaggcagga tcgaccccct ggtgtacctg
     6661 gacgagttcc tgaggaacag cgagcccgtg ctgaagagag tgaacaggac cggcaacagc
     6721 agcagcaaca agcaggagta ccagctgctg aaggacaacc tggtgcgcag ctacaacaag
     6781 gccctgaaga agaacgcccc ctaccccatc ttcgctatca agaacggccc taagagccac
     6841 atcggcaggc acctgatgac cagctttctg agcatgaagg gcctgaccga gctgacaaac
     6901 gtggtgggca actggagcga caagagggcc tccgccgtgg ccaggaccac ctacacccac
     6961 cagatcaccg ccatccccga ccactacttc gccctggtgt ccaggtacta cgcctacgac
     7021 cccatcagca aggagatgat cgccctgaag gacgagacta accccatcga ggagtggcag
     7081 cacatcgagc agctgaaggg cagcgccgag ggcagcctct ccggagggtc tggctccgga
     7141 tcaagtggtg gcagcggtac ttcgagcaca accaaaaaag ccagtgattc tgcattctgg
     7201 cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag ctttcctgac
     7261 ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt ctgcatagcc
     7321 agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg tcaggtgttt
     7381 ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg ttccgttgtg
     7441 gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gccccaagaa aaagcggaag
     7501 gtgtaggctt ctctagctag agtcgatcga caagctcgag tttctccata ataatgtgtg
     7561 agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata
     7621 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat
     7681 tcctaaaacc aaaatccagt actaaaatcc agatcgcttt acgaattccc atggggagcc
     7741 aacactcgaa tccccacccg ttggaccaaa cccggctcat taagcgtcgg ttcagattta
     7801 tttcctttat ttaaaaaaaa ggaaagggta aaaaatagaa aattggaaac agttaaagcc
     7861 caaaattgta atttaccgag aattgtaaat ttacctgaaa accctacgct atagtttcga
     7921 ctataaatac caaacttagg acctcacttc agaatcccct cgtcgctgcg tctctctccc
     7981 gcaaccttcg attttcgttt attcgcatcc atcggagaga gaaaacaatc aataagcgac
     8041 ctactgtatt tttacaacaa ttaccaacaa caacaaacaa caaacaacat tacaattact
     8101 atttacaatt acaatgagca caaccaaaaa agccagtgat tctgcattct ggcttgaggt
     8161 tgaaggtaat tccatgaccg caccaacagg ctccaagcca agctttcctg acggaatgtt
     8221 aattctcgtt gaccctgagc aggctgttga gccaggtgat ttctgcatag ccagacttgg
     8281 gggtgatgag tttaccttca agaaactgat cagggatagc ggtcaggtgt ttttacaacc
     8341 actaaaccca cagtacccaa tgatcccatg caatgagagt tgttccgttg tggggaaagt
     8401 tatcgctagt cagtggcctg aagagacgtt tggcagcctc tccggagggt ctggctccgg
     8461 atcaagtggt ggcagcggta cttcgagcat cagatacccc gcctggaacg gcatcatcag
     8521 ccaggaggtg ctggactacc tgagcagcta catcaacagg cggatcccca agaaaaagcg
     8581 gaaggtgtga gcttctctag ctagagtcga tcgacaagct cgagtttctc cataataatg
     8641 tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc atgtgttgag
     8701 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
     8761 taattcctaa aaccaaaatc cagtactaaa atccagatcg ctcagatgag accgcagctg
     8821 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     8881 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     8941 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     9001 tgcatgcctg caggtcgact ctagaggatc cccgggtacc gagctcgaat tcactggccg
     9061 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     9121 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     9181 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
     9241 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     9301 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     9361 tcccggcatc cgcttacaga caagctgtga cggtctcagg gacacgaagt gatccgttta
     9421 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     9481 agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg
     9541 ccagccgcct ttgcgacgct caccgggctg gttgccctcg ccgctgggct ggcggccgtc
     9601 tatggccctg caaacgcgcc agaaacgccg tcgaagccgt gtgcgagaca ccgcggccgc
     9661 cggcgttgtg gatacctcgc ggaaaacttg gccctcactg acagatgagg ggcggacgtt
     9721 gacacttgag gggccgactc acccggcgcg gcgttgacag atgaggggca ggctcgattt
     9781 cggccggcga cgtggagctg gccagcctcg caaatcggcg aaaacgcctg attttacgcg
     9841 agtttcccac agatgatgtg gacaagcctg gggataagtg ccctgcggta ttgacacttg
     9901 aggggcgcga ctactgacag atgaggggcg cgatccttga cacttgaggg gcagagtgct
     9961 gacagatgag gggcgcacct attgacattt gaggggctgt ccacaggcag aaaatccagc
    10021 atttgcaagg gtttccgccc gtttttcggc caccgctaac ctgtctttta acctgctttt
    10081 aaaccaatat ttataaacct tgtttttaac cagggctgcg ccctgtgcgc gtgaccgcgc
    10141 acgccgaagg ggggtgcccc cccttctcga accctcccgg cccgctaacg cgggcctccc
    10201 atccccccag gggctgcgcc cctcggccgc gaacggcctc accccaaaaa tggcagcgct
    10261 ggccaattcc cgagtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
    10321 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
    10381 tatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat accgctgcgt
    10441 aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa
    10501 cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg tggaacggga
    10561 aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga
    10621 acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc tttgctcgga
    10681 agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag
    10741 gctctttcac tccatcgaca tatcggattg tccctatacg aatagcttag acagccgctt
    10801 agccgaattg gattacttac tgaataacga tctggccgat gtggattgcg aaaactggga
    10861 agaggacact ccatttaaag atccgcgcga gctgtatgat tttttaaaga cggaaaagcc
    10921 cgaagaggaa cttgtctttt cccacggcga cctgggagac agcaacatct ttgtgaaaga
    10981 tggcaaagta agtggcttta ttgatcttgg gagaagcggc agggcggaca agtggtatga
    11041 cattgccttc tgcgtccggt cgctcaggga ggatatcggg gaagaacagt atgtcgagct
    11101 attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatt atattttact
    11161 ggatgaattg ttttagctgt cagaccaagt ttactcatat atactttaga ttgatttaaa
    11221 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
    11281 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    11341 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
    11401 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
    11461 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
    11521 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
    11581 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
    11641 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
    11701 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
    11761 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
    11821 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
    11881 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
    11941 cagcaacgcg gcctttttac ggttcctggc agatcctaga tgtggcgcaa cgatgccggc
    12001 gacaagcagg agcgcaccga cttcttccgc atcaagtgtt ttggctctca ggccgaggcc
    12061 cacggcaagt atttgggcaa ggggtcgctg gtattcgtgc agggcaagat tcggaatacc
    12121 aagtacgaga aggacggcca gacggtctac gggaccgact tcattgccga taaggtggat
    12181 tatctggaca ccaaggcacc aggcgggtca aatcaggaat aagggcacat tgccccggcg
    12241 tgagtcgggg caatcccgca aggagggtga atgaatcgga cgtttgaccg gaaggcatac
    12301 aggcaagaac tgatcgacgc ggggttttcc gccgaggatg ccgaaaccat cgcaagccgc
    12361 accgtcatgc gtgcgccccg cgaaaccttc cagtccgtcg gctcgatggt ccagcaagct
    12421 acggccaaga tcgagcgcga cagcgtgcaa ctggctcccc ctgccctgcc cgcgccatcg
    12481 gccgccgtgg agcgttcgcg tcgtcttgaa caggaggcgg caggtttggc gaagtcgatg
    12541 accatcgaca cgcgaggaac tatgacgacc aagaagcgaa aaaccgccgg cgaggacctg
    12601 gcaaaacagg tcagcgaggc caagcaggcc gcgttgctga aacacacgaa gcagcagatc
    12661 aaggaaatgc agctttcctt gttcgatatt gcgccgtggc cggacacgat gcgagcgatg
    12721 ccaaacgaca cggcccgctc tgccctgttc accacgcgca acaagaaaat cccgcgcgag
    12781 gcgctgcaaa acaaggtcat tttccacgtc aacaaggacg tgaagatcac ctacaccggc
    12841 gtcgagctgc gggccgacga tgacgaactg gtgtggcagc aggtgttgga gtacgcgaag
    12901 cgcaccccta tcggcgagcc gatcaccttc acgttctacg agctttgcca ggacctgggc
    12961 tggtcgatca atggccggta ttacacgaag gccgaggaat gcctgtcgcg cctacaggcg
    13021 acggcgatgg gcttcacgtc cgaccgcgtt gggcacctgg aatcggtgtc gctgctgcac
    13081 cgcttccgcg tcctggaccg tggcaagaaa acgtcccgtt gccaggtcct gatcgacgag
    13141 gaaatcgtcg tgctgtttgc tggcgaccac tacacgaaat tcatatggga gaagtaccgc
    13201 aagctgtcgc cgacggcccg acggatgttc gactatttca gctcgcaccg ggagccgtac
    13261 ccgctcaagc tggaaacctt ccgcctcatg tgcggatcgg attccacccg cgtgaagaag
    13321 tggcgcgagc aggtcggcga agcctgcgaa gagttgcgag gcagcggcct ggtggaacac
    13381 gcctgggtca atgatgacct ggtgcattgc aaacgctagg gccttgtggg gtcagttccg
    13441 gctgggggtt cagcagcccc tgctcggatc tgttggaccg gacagtagtc atggttgatg
    13501 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
    13561 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
    13621 tgcggacgtt tttaatgtac tggggttgaa cactct
//
