LOCUS       sequence-377975-       10571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as a 1-input state of the split-NAND gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10571)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 10571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP716_L2sV3_NAND_1IF2_FRT_Rluc_FlpF2_C1_lacZ
FEATURES             Location/Qualifiers
     source          1..10571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    22..55
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    843..876
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    892..947
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     intron          979..1167
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2195..2369
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             2757..4409
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(2823..2841)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      4429..4629
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    4972..5027
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5482..5502
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5540..5714
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    5784..5805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5820..5825,5826..5843,5844..5850)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5858..5874
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5863..5885
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5882..5898
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5882..5898
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5894..6217
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    5911..5967
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(5968..5985)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5968..5984)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5977..5999)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6193..6212)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    6349..6373
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      6461..7171
                     /label=oriV
                     /note="incP origin of replication"
     promoter        7192..7296
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7297..8091
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      8262..8850
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8751..8770
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             9186..10334
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    10480..10504
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga ggaagttcct attctctaga aagtatagga acttctcgac
       61 aaaatttaga acgaacttaa ttatgatctc aaatacattg atacatatct catctagatc
      121 taggttatca ttatgtaaga aagttttgac gaatatggca cgacaaaatg gctagactcg
      181 atgtaattgg tatctcaact caacattata cttataccaa acattagtta gacaaaattt
      241 aaacaactat tttttatgta tgcaagagtc agcatatgta taattgattc agaatcgttt
      301 tgacgagttc ggatgtagta gtagccatta tttaatgtac atactaatcg tgaatagtga
      361 atatgatgaa acattgtatc ttattgtata aatatccata aacacatcat gaaagacact
      421 ttctttcacg gtctgaatta attatgatac aattctaata gaaaacgaat taaattacgt
      481 tgaattgtat gaaatctaat tgaacaagcc aaccacgacg acgactaacg ttgcctggat
      541 tgactcggtt taagttaacc actaaaaaaa cggagctgtc atgtaacacg cggatcgagc
      601 aggtcacagt catgaagcca tcaaagcaaa agaactaatc caagggctga gatgattaat
      661 tagtttaaaa attagttaac acgagggaaa aggctgtctg acagccaggt cacgttatct
      721 ttacctgtgg tcgaaatgat tcgtgtctgt cgattttaat tatttttttg aaaggccgaa
      781 aataaagttg taagagataa acccgcctat ataaattcat atattttcct ctccgctttg
      841 aagaagttcc tattctctag aaagtatagg aacttctact gtatttttac aacaattacc
      901 aacaacaaca aacaacaaac aacattacaa ttactattta caattacaat gacttcaaag
      961 gtgtatgatc ctgagcaggt aagtttctgc ttctaccttt gatatatata taataattat
     1021 cattaattag tagtaatata atatttcaaa tatttttttc aaaataaaag aatgtagtat
     1081 atagcaattg cttttctgta gtttataagt gtgtatattt taatttataa cttttctaat
     1141 atatgaccaa aatttgttga tgtgcagaga aaaagaatga taactggtcc acaatggtgg
     1201 gcaagatgta aacagatgaa cgtgcttgat tctttcatta attactacga ttcagaaaag
     1261 cacgctgaga atgcagttat ctttcttcac ggaaacgctg catcttcata tttgtggaga
     1321 catgttgtgc ctcacattga accagtggct aggtgtatta tcccagatct tatcggaatg
     1381 ggaaagtctg gtaaatctgg aaatggttct tacagacttt tggatcatta taagtacctc
     1441 acagcttggt tcgaacttct caacttacct aagaagatta ttttcgttgg acacgattgg
     1501 ggtgcttgct tggcattcca ttactcatac gaacaccaag ataagatcaa agctatagtt
     1561 catgcagaga gtgttgtgga tgtgatagaa tcttgggatg agtggccaga tattgaagag
     1621 gatattgctt tgatcaagtc tgaagaggga gaaaagatgg ttctcgaaaa taactttttc
     1681 gtggaaacta tgcttccttc aaagattatg agaaaattgg aaccagaaga gtttgctgca
     1741 tatctcgaac ctttcaagga aaaaggagag gttagaaggc caacactcag ttggcctagg
     1801 gaaataccat tagttaaggg aggtaaacct gatgttgtgc agatcgtgag aaattataac
     1861 gcttacctta gggcatcaga tgatttgcct aagatgttca tcgagtcaga tccaggattt
     1921 ttcagtaatg ctatagttga aggtgcaaag aaatttccaa acaccgagtt cgttaaggtg
     1981 aaaggattgc atttctcaca agaagatgct cctgatgaga tgggaaagta tattaagtct
     2041 tttgttgaaa gagtgctcaa aaacgagcag ggttctcgat ctgatgcttc tgaagtaacg
     2101 tcaacaagct cccagtctga ggtatgcaca gtggaaacgc caggttgtgt tggttcttaa
     2161 gcttctctag ctagagtcga tcgacaagct cgagtttctc cataataatg tgtgagtagt
     2221 tcccagataa gggaattagg gttcctatag ggtttcgctc atgtgttgag catataagaa
     2281 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
     2341 aaccaaaatc cagtactaaa atccagatcg ctgcaagaat tcaagcttgg agccaacact
     2401 cgaatcccca cccgttggac caaacccggc tcattaagcg tcggttcaga tttatttcct
     2461 ttatttaaaa aaaaggaaag ggtaaaaaat agaaaattgg aaacagttaa agcccaaaat
     2521 tgtaatttac cgagaattgt aaatttacct gaaaacccta cgctatagtt tcgactataa
     2581 ataccaaact taggacctca cttcagaatc ccctcgtcgc tgcgtctctc tcccgcaacc
     2641 ttcgattttc gtttattcgc atccatcgga gagagaaaac aatcaataag cgacctacta
     2701 caaacaagta agtaagagaa aaaccaaaag aagaagagaa acaacaagaa gaagtaatgg
     2761 aggacgccaa aaacataaag aaaggcccgg cgccattcta tcctctagag gatggaaccg
     2821 ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt
     2881 ttacagatgc acatatcgag gtgaacatca cgtacgcgga atacttcgaa atgtccgttc
     2941 ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca
     3001 gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag
     3061 ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg aacatttcgc
     3121 agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa
     3181 aattaccaat aatccagaaa attattatca tggattctaa aacggattac cagggatttc
     3241 agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg
     3301 taccagagtc ctttgatcgt gacaaaacaa ttgcactgat aatgaattcc tctggatcta
     3361 ctgggttacc taagggtgtg gcccttccgc atagaactgc ctgcgtcaga ttctcgcatg
     3421 ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc
     3481 cattccatca cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag
     3541 tcgtcttaat gtatagattt gaagaagagc tgtttttacg atcccttcag gattacaaaa
     3601 ttcaaagtgc gttgctagta ccaaccctat tttcattctt cgccaaaagc actctgattg
     3661 acaaatacga tttatctaat ttacacgaaa ttgcttctgg gggcgcacct ctttcgaaag
     3721 aagtcgggga agcggttgca aaacgcttcc atcttccagg gatacgacaa ggatatgggc
     3781 tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg
     3841 tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc
     3901 tgggcgttaa tcagagaggc gaattatgtg tcagaggacc tatgattatg tccggttatg
     3961 taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag
     4021 acatagctta ctgggacgag gacgaacact tcttcatagt tgaccgcttg aagtctttaa
     4081 ttaaatacaa aggatatcag gtggcccccg ctgaattgga atcgatattg ttacaacacc
     4141 ccaacatctt cgacgcgggc gtggcaggtc tgcccgacga tgacgccggt gaacttcccg
     4201 ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg
     4261 tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag
     4321 taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg
     4381 ccaagaaggg cggaaagtcc aaattgtaag cttgatcccc cgtcgacagc tagctatatc
     4441 atcaatttat gtattacaca taatatcgca ctcagtcttt catctacggc aatgtaccag
     4501 ctgatataat cagttattga aatatttctg aatttaaact tgcatcaata aatttatgtt
     4561 tttgcttgga ctataatacc tgacttgtta ttttatcaat aaatatttaa actatatttc
     4621 tttcaagatc gctactagaa ttcgagctcg gagccaacac tcgaatcccc acccgttgga
     4681 ccaaacccgg ctcattaagc gtcggttcag atttatttcc tttatttaaa aaaaaggaaa
     4741 gggtaaaaaa tagaaaattg gaaacagtta aagcccaaaa ttgtaattta ccgagaattg
     4801 taaatttacc tgaaaaccct acgctatagt ttcgactata aataccaaac ttaggacctc
     4861 acttcagaat cccctcgtcg ctgcgtctct ctcccgcaac cttcgatttt cgtttattcg
     4921 catccatcgg agagagaaaa caatcaataa gcgacctact gtatttttac aacaattacc
     4981 aacaacaaca aacaacaaac aacattacaa ttactattta caattacaat gagcacaacc
     5041 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca
     5101 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct
     5161 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa
     5221 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc
     5281 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag
     5341 acgtttggca gcctctccgg agggtctggc tccggatcaa gtggtggcag cggtacttcg
     5401 agcatcagat accccgcctg gaacggcatc atcagccagg aggtgctgga ctacctgagc
     5461 agctacatca acaggcggat ccccaagaaa aagcggaagg tgtgagcttc tctagctaga
     5521 gtcgatcgac aagctcgagt ttctccataa taatgtgtga gtagttccca gataagggaa
     5581 ttagggttcc tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat
     5641 ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagta
     5701 ctaaaatcca gatcgcttta ctgagaccgc agctggcacg acaggtttcc cgactggaaa
     5761 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     5821 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     5881 acaggaaaca gctatgacca tgattacgcc aagcttgcat gcctgcaggt cgactctaga
     5941 ggatccccgg gtaccgagct cgaattcact ggccgtcgtt ttacaacgtc gtgactggga
     6001 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     6061 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     6121 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     6181 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     6241 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     6301 tgtgacggtc tcagggacac gaagtgatcc gtttaaacta tcagtgtttg acaggatata
     6361 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
     6421 gtgaaaaggt ttatccgttc gtccatttgt atgtgccagc cgcctttgcg acgctcaccg
     6481 ggctggttgc cctcgccgct gggctggcgg ccgtctatgg ccctgcaaac gcgccagaaa
     6541 cgccgtcgaa gccgtgtgcg agacaccgcg gccgccggcg ttgtggatac ctcgcggaaa
     6601 acttggccct cactgacaga tgaggggcgg acgttgacac ttgaggggcc gactcacccg
     6661 gcgcggcgtt gacagatgag gggcaggctc gatttcggcc ggcgacgtgg agctggccag
     6721 cctcgcaaat cggcgaaaac gcctgatttt acgcgagttt cccacagatg atgtggacaa
     6781 gcctggggat aagtgccctg cggtattgac acttgagggg cgcgactact gacagatgag
     6841 gggcgcgatc cttgacactt gaggggcaga gtgctgacag atgaggggcg cacctattga
     6901 catttgaggg gctgtccaca ggcagaaaat ccagcatttg caagggtttc cgcccgtttt
     6961 tcggccaccg ctaacctgtc ttttaacctg cttttaaacc aatatttata aaccttgttt
     7021 ttaaccaggg ctgcgccctg tgcgcgtgac cgcgcacgcc gaaggggggt gccccccctt
     7081 ctcgaaccct cccggcccgc taacgcgggc ctcccatccc cccaggggct gcgcccctcg
     7141 gccgcgaacg gcctcacccc aaaaatggca gcgctggcca attcccgagt gcgcggaacc
     7201 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     7261 tgataaatgc ttcaataata ttgaaaaagg aagagtatgg ctaaaatgag aatatcaccg
     7321 gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct
     7381 gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc
     7441 cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa
     7501 ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg
     7561 ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct
     7621 gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg
     7681 gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat
     7741 aacgatctgg ccgatgtgga ttgcgaaaac tgggaagagg acactccatt taaagatccg
     7801 cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac
     7861 ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat
     7921 cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgctc
     7981 agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag
     8041 cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta gctgtcagac
     8101 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     8161 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     8221 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     8281 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     8341 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     8401 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     8461 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     8521 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     8581 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     8641 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     8701 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     8761 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     8821 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8881 ctggcagatc ctagatgtgg cgcaacgatg ccggcgacaa gcaggagcgc accgacttct
     8941 tccgcatcaa gtgttttggc tctcaggccg aggcccacgg caagtatttg ggcaaggggt
     9001 cgctggtatt cgtgcagggc aagattcgga ataccaagta cgagaaggac ggccagacgg
     9061 tctacgggac cgacttcatt gccgataagg tggattatct ggacaccaag gcaccaggcg
     9121 ggtcaaatca ggaataaggg cacattgccc cggcgtgagt cggggcaatc ccgcaaggag
     9181 ggtgaatgaa tcggacgttt gaccggaagg catacaggca agaactgatc gacgcggggt
     9241 tttccgccga ggatgccgaa accatcgcaa gccgcaccgt catgcgtgcg ccccgcgaaa
     9301 ccttccagtc cgtcggctcg atggtccagc aagctacggc caagatcgag cgcgacagcg
     9361 tgcaactggc tccccctgcc ctgcccgcgc catcggccgc cgtggagcgt tcgcgtcgtc
     9421 ttgaacagga ggcggcaggt ttggcgaagt cgatgaccat cgacacgcga ggaactatga
     9481 cgaccaagaa gcgaaaaacc gccggcgagg acctggcaaa acaggtcagc gaggccaagc
     9541 aggccgcgtt gctgaaacac acgaagcagc agatcaagga aatgcagctt tccttgttcg
     9601 atattgcgcc gtggccggac acgatgcgag cgatgccaaa cgacacggcc cgctctgccc
     9661 tgttcaccac gcgcaacaag aaaatcccgc gcgaggcgct gcaaaacaag gtcattttcc
     9721 acgtcaacaa ggacgtgaag atcacctaca ccggcgtcga gctgcgggcc gacgatgacg
     9781 aactggtgtg gcagcaggtg ttggagtacg cgaagcgcac ccctatcggc gagccgatca
     9841 ccttcacgtt ctacgagctt tgccaggacc tgggctggtc gatcaatggc cggtattaca
     9901 cgaaggccga ggaatgcctg tcgcgcctac aggcgacggc gatgggcttc acgtccgacc
     9961 gcgttgggca cctggaatcg gtgtcgctgc tgcaccgctt ccgcgtcctg gaccgtggca
    10021 agaaaacgtc ccgttgccag gtcctgatcg acgaggaaat cgtcgtgctg tttgctggcg
    10081 accactacac gaaattcata tgggagaagt accgcaagct gtcgccgacg gcccgacgga
    10141 tgttcgacta tttcagctcg caccgggagc cgtacccgct caagctggaa accttccgcc
    10201 tcatgtgcgg atcggattcc acccgcgtga agaagtggcg cgagcaggtc ggcgaagcct
    10261 gcgaagagtt gcgaggcagc ggcctggtgg aacacgcctg ggtcaatgat gacctggtgc
    10321 attgcaaacg ctagggcctt gtggggtcag ttccggctgg gggttcagca gcccctgctc
    10381 ggatctgttg gaccggacag tagtcatggt tgatgggctg cctgtatcga gtggtgattt
    10441 tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt
    10501 aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgggg
    10561 ttgaacactc t
//
