LOCUS       sequence-378012-       10261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as the 0-input state of the A NIMPLY B gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10261)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 10261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP763_L2pV3_ANIMPLYB_B3RT_FRT_OCS_Rluc_0I_lacZ
FEATURES             Location/Qualifiers
     source          1..10261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    911..944
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      945..1652
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1653..1686
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          1753..1941
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2969..3143
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3531..5183
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3597..3615)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5203..5403
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     protein_bind    5474..5495
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5510..5515,5516..5533,5534..5540)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5548..5564
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5553..5575
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5572..5588
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5572..5588
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5584..5907
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    5601..5657
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(5658..5675)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5658..5674)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5667..5689)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5883..5902)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    6039..6063
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      6151..6861
                     /label=oriV
                     /note="incP origin of replication"
     promoter        6882..6986
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6987..7781
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7952..8540
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8441..8460
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8876..10024
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    10170..10194
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gggttgctta agaataagta attcttaagc aacctcgaca
       61 aaatttagaa cgaacttaat tatgatctca aatacattga tacatatctc atctagatct
      121 aggttatcat tatgtaagaa agttttgacg aatatggcac gacaaaatgg ctagactcga
      181 tgtaattggt atctcaactc aacattatac ttataccaaa cattagttag acaaaattta
      241 aacaactatt ttttatgtat gcaagagtca gcatatgtat aattgattca gaatcgtttt
      301 gacgagttcg gatgtagtag tagccattat ttaatgtaca tactaatcgt gaatagtgaa
      361 tatgatgaaa cattgtatct tattgtataa atatccataa acacatcatg aaagacactt
      421 tctttcacgg tctgaattaa ttatgataca attctaatag aaaacgaatt aaattacgtt
      481 gaattgtatg aaatctaatt gaacaagcca accacgacga cgactaacgt tgcctggatt
      541 gactcggttt aagttaacca ctaaaaaaac ggagctgtca tgtaacacgc ggatcgagca
      601 ggtcacagtc atgaagccat caaagcaaaa gaactaatcc aagggctgag atgattaatt
      661 agtttaaaaa ttagttaaca cgagggaaaa ggctgtctga cagccaggtc acgttatctt
      721 tacctgtggt cgaaatgatt cgtgtctgtc gattttaatt atttttttga aaggccgaaa
      781 ataaagttgt aagagataaa cccgcctata taaattcata tattttcctc tccgctttga
      841 aggttgctta agaataagta attcttaagc aacctactgt atttttacaa caattaccaa
      901 caacaacaaa gaagttccta ttctctagaa agtataggaa cttcctgctt taatgagata
      961 tgcgagaagc ctatgatcgc atgatatttg ctttcaattc tgttgtgcac gttgtaaaaa
     1021 acctgagcat gtgtagctca gatccttacc gccggtttcg gttcattcta atgaatatat
     1081 cacccgttac tatcgtattt ttatgaataa tattctccgt tcaatttact gattgtaccc
     1141 tactacttat atgtacaata ttaaaatgaa aacaatatat tgtgctgaat aggtttatag
     1201 cgacatctat gatagagcgc cacaataaca aacaattgcg ttttattatt acaaatccaa
     1261 ttttaaaaaa agcggcagaa ccggtcaaac ctaaaagact gattacataa atcttattca
     1321 aatttcaaaa gtgccccagg ggctagtatc tacgacacac cgagcggcga actaataacg
     1381 ctcactgaag ggaactccgg ttccccgccg gcgcgcatgg gtgagattcc ttgaagttga
     1441 gtattggccg tccgctctac cgaaagttac gggcaccatt caacccggtc cagcacggcg
     1501 gccgggtaac cgacttgctg ccccgagaat tatgcagcat ttttttggtg tatgtgggcc
     1561 ccaaatgaag tgcaggtcaa accttgacag tgacgacaaa tcgttgggcg ggtccagggc
     1621 gaattttgcg acaacatgtc gaggctcagc aggaagttcc tattctctag aaagtatagg
     1681 aacttccaac aaacaacatt acaattacta tttacaatta caatgacttc aaaggtgtat
     1741 gatcctgagc aggtaagttt ctgcttctac ctttgatata tatataataa ttatcattaa
     1801 ttagtagtaa tataatattt caaatatttt tttcaaaata aaagaatgta gtatatagca
     1861 attgcttttc tgtagtttat aagtgtgtat attttaattt ataacttttc taatatatga
     1921 ccaaaatttg ttgatgtgca gagaaaaaga atgataactg gtccacaatg gtgggcaaga
     1981 tgtaaacaga tgaacgtgct tgattctttc attaattact acgattcaga aaagcacgct
     2041 gagaatgcag ttatctttct tcacggaaac gctgcatctt catatttgtg gagacatgtt
     2101 gtgcctcaca ttgaaccagt ggctaggtgt attatcccag atcttatcgg aatgggaaag
     2161 tctggtaaat ctggaaatgg ttcttacaga cttttggatc attataagta cctcacagct
     2221 tggttcgaac ttctcaactt acctaagaag attattttcg ttggacacga ttggggtgct
     2281 tgcttggcat tccattactc atacgaacac caagataaga tcaaagctat agttcatgca
     2341 gagagtgttg tggatgtgat agaatcttgg gatgagtggc cagatattga agaggatatt
     2401 gctttgatca agtctgaaga gggagaaaag atggttctcg aaaataactt tttcgtggaa
     2461 actatgcttc cttcaaagat tatgagaaaa ttggaaccag aagagtttgc tgcatatctc
     2521 gaacctttca aggaaaaagg agaggttaga aggccaacac tcagttggcc tagggaaata
     2581 ccattagtta agggaggtaa acctgatgtt gtgcagatcg tgagaaatta taacgcttac
     2641 cttagggcat cagatgattt gcctaagatg ttcatcgagt cagatccagg atttttcagt
     2701 aatgctatag ttgaaggtgc aaagaaattt ccaaacaccg agttcgttaa ggtgaaagga
     2761 ttgcatttct cacaagaaga tgctcctgat gagatgggaa agtatattaa gtcttttgtt
     2821 gaaagagtgc tcaaaaacga gcagggttct cgatctgatg cttctgaagt aacgtcaaca
     2881 agctcccagt ctgaggtatg cacagtggaa acgccaggtt gtgttggttc ttaagcttct
     2941 ctagctagag tcgatcgaca agctcgagtt tctccataat aatgtgtgag tagttcccag
     3001 ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata agaaaccctt
     3061 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
     3121 aatccagtac taaaatccag atcgctgcaa gaattcaagc ttggagccaa cactcgaatc
     3181 cccacccgtt ggaccaaacc cggctcatta agcgtcggtt cagatttatt tcctttattt
     3241 aaaaaaaagg aaagggtaaa aaatagaaaa ttggaaacag ttaaagccca aaattgtaat
     3301 ttaccgagaa ttgtaaattt acctgaaaac cctacgctat agtttcgact ataaatacca
     3361 aacttaggac ctcacttcag aatcccctcg tcgctgcgtc tctctcccgc aaccttcgat
     3421 tttcgtttat tcgcatccat cggagagaga aaacaatcaa taagcgacct actacaaaca
     3481 agtaagtaag agaaaaacca aaagaagaag agaaacaaca agaagaagta atggaggacg
     3541 ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga accgctggag
     3601 agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag
     3661 atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc gttcggttgg
     3721 cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa
     3781 actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc
     3841 ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt tcgcagccta
     3901 ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac
     3961 caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga
     4021 tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtaccag
     4081 agtcctttga tcgtgacaaa acaattgcac tgataatgaa ttcctctgga tctactgggt
     4141 tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg catgccagag
     4201 atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc
     4261 atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct
     4321 taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac aaaattcaaa
     4381 gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg attgacaaat
     4441 acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg aaagaagtcg
     4501 gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat gggctcactg
     4561 agactacatc agctattctg attacacccg agggggatga taaaccgggc gcggtcggta
     4621 aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg
     4681 ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt tatgtaaaca
     4741 atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag
     4801 cttactggga cgaggacgaa cacttcttca tagttgaccg cttgaagtct ttaattaaat
     4861 acaaaggata tcaggtggcc cccgctgaat tggaatcgat attgttacaa caccccaaca
     4921 tcttcgacgc gggcgtggca ggtctgcccg acgatgacgc cggtgaactt cccgccgccg
     4981 ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca
     5041 gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga
     5101 aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga
     5161 agggcggaaa gtccaaattg taagcttgat cccccgtcga cagctagcta tatcatcaat
     5221 ttatgtatta cacataatat cgcactcagt ctttcatcta cggcaatgta ccagctgata
     5281 taatcagtta ttgaaatatt tctgaattta aacttgcatc aataaattta tgtttttgct
     5341 tggactgtaa tacctgactt gttattttat caataaatat ttaaactata tttctttcaa
     5401 gatcgctact atgagaccgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     5461 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     5521 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     5581 gctatgacca tgattacgcc aagcttgcat gcctgcaggt cgactctaga ggatccccgg
     5641 gtgccgagct cgaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     5701 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     5761 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
     5821 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     5881 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     5941 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgacggtc
     6001 tcagggacac gaagtgatcc gtttaaacta tcagtgtttg acaggatata ttggcgggta
     6061 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
     6121 ttatccgttc gtccatttgt atgtgccagc cgcctttgcg acgctcaccg ggctggttgc
     6181 cctcgccgct gggctggcgg ccgtctatgg ccctgcaaac gcgccagaaa cgccgtcgaa
     6241 gccgtgtgcg agacaccgcg gccgccggcg ttgtggatac ctcgcggaaa acttggccct
     6301 cactgacaga tgaggggcgg acgttgacac ttgaggggcc gactcacccg gcgcggcgtt
     6361 gacagatgag gggcaggctc gatttcggcc ggcgacgtgg agctggccag cctcgcaaat
     6421 cggcgaaaac gcctgatttt acgcgagttt cccacagatg atgtggacaa gcctggggat
     6481 aagtgccctg cggtattgac acttgagggg cgcgactact gacagatgag gggcgcgatc
     6541 cttgacactt gaggggcaga gtgctgacag atgaggggcg cacctattga catttgaggg
     6601 gctgtccaca ggcagaaaat ccagcatttg caagggtttc cgcccgtttt tcggccaccg
     6661 ctaacctgtc ttttaacctg cttttaaacc aatatttata aaccttgttt ttaaccaggg
     6721 ctgcgccctg tgcgcgtgac cgcgcacgcc gaaggggggt gccccccctt ctcgaaccct
     6781 cccggcccgc taacgcgggc ctcccatccc cccaggggct gcgcccctcg gccgcgaacg
     6841 gcctcacccc aaaaatggca gcgctggcca attcccgagt gcgcggaacc cctatttgtt
     6901 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6961 ttcaataata ttgaaaaagg aagagtatgg ctaaaatgag aatatcaccg gaattgaaaa
     7021 aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct gctaaggtat
     7081 ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc cggtataaag
     7141 ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa ggaaagctgc
     7201 ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg ctcatgagtg
     7261 aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct gaaaagatta
     7321 tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg gattgtccct
     7381 atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat aacgatctgg
     7441 ccgatgtgga ttgcgaaaac tgggaagagg acactccatt taaagatccg cgcgagctgt
     7501 atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac ggcgacctgg
     7561 gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat cttgggagaa
     7621 gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgctc agggaggata
     7681 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
     7741 agaaaataaa atattatatt ttactggatg aattgtttta gctgtcagac caagtttact
     7801 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7861 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7921 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7981 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8041 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     8101 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8161 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8221 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8281 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8341 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8401 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8461 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8521 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcagatc
     8581 ctagatgtgg cgcaacgatg ccggcgacaa gcaggagcgc accgacttct tccgcatcaa
     8641 gtgttttggc tctcaggccg aggcccacgg caagtatttg ggcaaggggt cgctggtatt
     8701 cgtgcagggc aagattcgga ataccaagta cgagaaggac ggccagacgg tctacgggac
     8761 cgacttcatt gccgataagg tggattatct ggacaccaag gcaccaggcg ggtcaaatca
     8821 ggaataaggg cacattgccc cggcgtgagt cggggcaatc ccgcaaggag ggtgaatgaa
     8881 tcggacgttt gaccggaagg catacaggca agaactgatc gacgcggggt tttccgccga
     8941 ggatgccgaa accatcgcaa gccgcaccgt catgcgtgcg ccccgcgaaa ccttccagtc
     9001 cgtcggctcg atggtccagc aagctacggc caagatcgag cgcgacagcg tgcaactggc
     9061 tccccctgcc ctgcccgcgc catcggccgc cgtggagcgt tcgcgtcgtc ttgaacagga
     9121 ggcggcaggt ttggcgaagt cgatgaccat cgacacgcga ggaactatga cgaccaagaa
     9181 gcgaaaaacc gccggcgagg acctggcaaa acaggtcagc gaggccaagc aggccgcgtt
     9241 gctgaaacac acgaagcagc agatcaagga aatgcagctt tccttgttcg atattgcgcc
     9301 gtggccggac acgatgcgag cgatgccaaa cgacacggcc cgctctgccc tgttcaccac
     9361 gcgcaacaag aaaatcccgc gcgaggcgct gcaaaacaag gtcattttcc acgtcaacaa
     9421 ggacgtgaag atcacctaca ccggcgtcga gctgcgggcc gacgatgacg aactggtgtg
     9481 gcagcaggtg ttggagtacg cgaagcgcac ccctatcggc gagccgatca ccttcacgtt
     9541 ctacgagctt tgccaggacc tgggctggtc gatcaatggc cggtattaca cgaaggccga
     9601 ggaatgcctg tcgcgcctac aggcgacggc gatgggcttc acgtccgacc gcgttgggca
     9661 cctggaatcg gtgtcgctgc tgcaccgctt ccgcgtcctg gaccgtggca agaaaacgtc
     9721 ccgttgccag gtcctgatcg acgaggaaat cgtcgtgctg tttgctggcg accactacac
     9781 gaaattcata tgggagaagt accgcaagct gtcgccgacg gcccgacgga tgttcgacta
     9841 tttcagctcg caccgggagc cgtacccgct caagctggaa accttccgcc tcatgtgcgg
     9901 atcggattcc acccgcgtga agaagtggcg cgagcaggtc ggcgaagcct gcgaagagtt
     9961 gcgaggcagc ggcctggtgg aacacgcctg ggtcaatgat gacctggtgc attgcaaacg
    10021 ctagggcctt gtggggtcag ttccggctgg gggttcagca gcccctgctc ggatctgttg
    10081 gaccggacag tagtcatggt tgatgggctg cctgtatcga gtggtgattt tgtgccgagc
    10141 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg
    10201 acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgggg ttgaacactc
    10261 t
//
