LOCUS       sequence-378111-       11786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as a 1-input state of the OR gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11786)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 11786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP760_L2pV1_OR-OCS_TCTP-Flp
FEATURES             Location/Qualifiers
     source          1..11786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    845..878
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(879..912)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      913..1620
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1621..1654
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(1655..1688)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          1755..1943
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2971..3145
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3533..5185
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3599..3617)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5205..5405
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    5748..5803
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5811..5831
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6515..6703
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    7327..7501
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    7564..7588
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      7676..8386
                     /label=oriV
                     /note="incP origin of replication"
     promoter        8407..8511
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8512..9306
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9477..10065
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9966..9985
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             10401..11549
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    11695..11719
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaagaagtt cctattctct agaaagtata ggaacttcat aacttcgtat agcatacatt
      901 atacgaagtt atctgcttta atgagatatg cgagaagcct atgatcgcat gatatttgct
      961 ttcaattctg ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga tccttaccgc
     1021 cggtttcggt tcattctaat gaatatatca cccgttacta tcgtattttt atgaataata
     1081 ttctccgttc aatttactga ttgtacccta ctacttatat gtacaatatt aaaatgaaaa
     1141 caatatattg tgctgaatag gtttatagcg acatctatga tagagcgcca caataacaaa
     1201 caattgcgtt ttattattac aaatccaatt ttaaaaaaag cggcagaacc ggtcaaacct
     1261 aaaagactga ttacataaat cttattcaaa tttcaaaagt gccccagggg ctagtatcta
     1321 cgacacaccg agcggcgaac taataacgct cactgaaggg aactccggtt ccccgccggc
     1381 gcgcatgggt gagattcctt gaagttgagt attggccgtc cgctctaccg aaagttacgg
     1441 gcaccattca acccggtcca gcacggcggc cgggtaaccg acttgctgcc ccgagaatta
     1501 tgcagcattt ttttggtgta tgtgggcccc aaatgaagtg caggtcaaac cttgacagtg
     1561 acgacaaatc gttgggcggg tccagggcga attttgcgac aacatgtcga ggctcagcag
     1621 gaagttccta ttctctagaa agtataggaa cttcataact tcgtatagca tacattatac
     1681 gaagttatca acaaacaaca ttacaattac tatttacaat tacaatgact tcaaaggtgt
     1741 atgatcctga gcaggtaagt ttctgcttct acctttgata tatatataat aattatcatt
     1801 aattagtagt aatataatat ttcaaatatt tttttcaaaa taaaagaatg tagtatatag
     1861 caattgcttt tctgtagttt ataagtgtgt atattttaat ttataacttt tctaatatat
     1921 gaccaaaatt tgttgatgtg cagagaaaaa gaatgataac tggtccacaa tggtgggcaa
     1981 gatgtaaaca gatgaacgtg cttgattctt tcattaatta ctacgattca gaaaagcacg
     2041 ctgagaatgc agttatcttt cttcacggaa acgctgcatc ttcatatttg tggagacatg
     2101 ttgtgcctca cattgaacca gtggctaggt gtattatccc agatcttatc ggaatgggaa
     2161 agtctggtaa atctggaaat ggttcttaca gacttttgga tcattataag tacctcacag
     2221 cttggttcga acttctcaac ttacctaaga agattatttt cgttggacac gattggggtg
     2281 cttgcttggc attccattac tcatacgaac accaagataa gatcaaagct atagttcatg
     2341 cagagagtgt tgtggatgtg atagaatctt gggatgagtg gccagatatt gaagaggata
     2401 ttgctttgat caagtctgaa gagggagaaa agatggttct cgaaaataac tttttcgtgg
     2461 aaactatgct tccttcaaag attatgagaa aattggaacc agaagagttt gctgcatatc
     2521 tcgaaccttt caaggaaaaa ggagaggtta gaaggccaac actcagttgg cctagggaaa
     2581 taccattagt taagggaggt aaacctgatg ttgtgcagat cgtgagaaat tataacgctt
     2641 accttagggc atcagatgat ttgcctaaga tgttcatcga gtcagatcca ggatttttca
     2701 gtaatgctat agttgaaggt gcaaagaaat ttccaaacac cgagttcgtt aaggtgaaag
     2761 gattgcattt ctcacaagaa gatgctcctg atgagatggg aaagtatatt aagtcttttg
     2821 ttgaaagagt gctcaaaaac gagcagggtt ctcgatctga tgcttctgaa gtaacgtcaa
     2881 caagctccca gtctgaggta tgcacagtgg aaacgccagg ttgtgttggt tcttaagctt
     2941 ctctagctag agtcgatcga caagctcgag tttctccata ataatgtgtg agtagttccc
     3001 agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata taagaaaccc
     3061 ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc
     3121 aaaatccagt actaaaatcc agatcgctgc aagaattcaa gcttggagcc aacactcgaa
     3181 tccccacccg ttggaccaaa cccggctcat taagcgtcgg ttcagattta tttcctttat
     3241 ttaaaaaaaa ggaaagggta aaaaatagaa aattggaaac agttaaagcc caaaattgta
     3301 atttaccgag aattgtaaat ttacctgaaa accctacgct atagtttcga ctataaatac
     3361 caaacttagg acctcacttc agaatcccct cgtcgctgcg tctctctccc gcaaccttcg
     3421 attttcgttt attcgcatcc atcggagaga gaaaacaatc aataagcgac ctactacaaa
     3481 caagtaagta agagaaaaac caaaagaaga agagaaacaa caagaagaag taatggagga
     3541 cgccaaaaac ataaagaaag gcccggcgcc attctatcct ctagaggatg gaaccgctgg
     3601 agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac
     3661 agatgcacat atcgaggtga acatcacgta cgcggaatac ttcgaaatgt ccgttcggtt
     3721 ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga
     3781 aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc
     3841 gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgaaca tttcgcagcc
     3901 taccgtagtg tttgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaaatt
     3961 accaataatc cagaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc
     4021 gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtacc
     4081 agagtccttt gatcgtgaca aaacaattgc actgataatg aattcctctg gatctactgg
     4141 gttacctaag ggtgtggccc ttccgcatag aactgcctgc gtcagattct cgcatgccag
     4201 agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt
     4261 ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt
     4321 cttaatgtat agatttgaag aagagctgtt tttacgatcc cttcaggatt acaaaattca
     4381 aagtgcgttg ctagtaccaa ccctattttc attcttcgcc aaaagcactc tgattgacaa
     4441 atacgattta tctaatttac acgaaattgc ttctgggggc gcacctcttt cgaaagaagt
     4501 cggggaagcg gttgcaaaac gcttccatct tccagggata cgacaaggat atgggctcac
     4561 tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg
     4621 taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg
     4681 cgttaatcag agaggcgaat tatgtgtcag aggacctatg attatgtccg gttatgtaaa
     4741 caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat
     4801 agcttactgg gacgaggacg aacacttctt catagttgac cgcttgaagt ctttaattaa
     4861 atacaaagga tatcaggtgg cccccgctga attggaatcg atattgttac aacaccccaa
     4921 catcttcgac gcgggcgtgg caggtctgcc cgacgatgac gccggtgaac ttcccgccgc
     4981 cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc
     5041 cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc
     5101 gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa
     5161 gaagggcgga aagtccaaat tgtaagcttg atcccccgtc gacagctagc tatatcatca
     5221 atttatgtat tacacataat atcgcactca gtctttcatc tacggcaatg taccagctga
     5281 tataatcagt tattgaaata tttctgaatt taaacttgca tcaataaatt tatgtttttg
     5341 cttggactat aatacctgac ttgttatttt atcaataaat atttaaacta tatttctttc
     5401 aagatcgcta ctagaattcg agctcggagc caacactcga atccccaccc gttggaccaa
     5461 acccggctca ttaagcgtcg gttcagattt atttccttta tttaaaaaaa aggaaagggt
     5521 aaaaaataga aaattggaaa cagttaaagc ccaaaattgt aatttaccga gaattgtaaa
     5581 tttacctgaa aaccctacgc tatagtttcg actataaata ccaaacttag gacctcactt
     5641 cagaatcccc tcgtcgctgc gtctctctcc cgcaaccttc gattttcgtt tattcgcatc
     5701 catcggagag agaaaacaat caataagcga cctactgtat ttttacaaca attaccaaca
     5761 acaacaaaca acaaacaaca ttacaattac tatttacaat tacaatggct cctaagaaga
     5821 agaggaaggt gatgagccag ttcgacatcc tgtgcaagac cccccccaag gtgctggtgc
     5881 ggcagttcgt ggagagattc gagaggccca gcggcgagaa gatcgccagc tgtgccgccg
     5941 agctgaccta cctgtgctgg atgatcaccc acaacggcac cgccatcaag agggccacct
     6001 tcatgagcta caacaccatc atcagcaaca gcctgagctt cgacatcgtg aacaagagcc
     6061 tgcagttcaa gtacaagacc cagaaggcca ccatcctgga ggccagcctg aagaagctga
     6121 tccccgcctg ggagttcacc atcatccctt acaacggcca gaagcaccag agcgacatca
     6181 ccgacatcgt gtccagcctg cagctgcagt tcgagagcag cgaggaggcc gacaagggca
     6241 acagccacag caagaagatg ctgaaggccc tgctgtccga gggcgagagc atctgggaga
     6301 tcaccgagaa gatcctgaac agcttcgagt acaccagcag gttcaccaag accaagaccc
     6361 tgtaccagtt cctgttcctg gccacattca tcaactgcgg caggttcagc gacatcaaga
     6421 acgtggaccc caagagcttc aagctggtgc agaacaagta cctgggcgtg atcattcagt
     6481 gcctggtgac cgagactaag acaagcgtgt ccaggtaagt ttctgcttct acctttgata
     6541 tatatataat aattatcatt aattagtagt aatataatat ttcaaatatt tttttcaaaa
     6601 taaaagaatg tagtatatag caattgcttt tctgtagttt ataagtgtgt atattttaat
     6661 ttataacttt tctaatatat gaccaaaatt tgttgatgtg caggcacatc tactttttca
     6721 gcgccagagg caggatcgac cccctggtgt acctggacga gttcctgagg aacagcgagc
     6781 ccgtgctgaa gagagtgaac aggaccggca acagcagcag caacaagcag gagtaccagc
     6841 tgctgaagga caacctggtg cgcagctaca acaaggccct gaagaagaac gccccctacc
     6901 ccatcttcgc tatcaagaac ggccctaaga gccacatcgg caggcacctg atgaccagct
     6961 ttctgagcat gaagggcctg accgagctga caaacgtggt gggcaactgg agcgacaaga
     7021 gggcctccgc cgtggccagg accacctaca cccaccagat caccgccatc cccgaccact
     7081 acttcgccct ggtgtccagg tactacgcct acgaccccat cagcaaggag atgatcgccc
     7141 tgaaggacga gactaacccc atcgaggagt ggcagcacat cgagcagctg aagggcagcg
     7201 ccgagggcag catcagatac cccgcctgga acggcatcat cagccaggag gtgctggact
     7261 acctgagcag ctacatcaac aggcggatct gagcttctct agctagagtc gatcgacaag
     7321 ctcgagtttc tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat
     7381 agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa
     7441 atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat
     7501 cgctttacga ggatgcacat gtgaccgagg gacacgaagt gatccgttta aactatcagt
     7561 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
     7621 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg ccagccgcct
     7681 ttgcgacgct caccgggctg gttgccctcg ccgctgggct ggcggccgtc tatggccctg
     7741 caaacgcgcc agaaacgccg tcgaagccgt gtgcgagaca ccgcggccgc cggcgttgtg
     7801 gatacctcgc ggaaaacttg gccctcactg acagatgagg ggcggacgtt gacacttgag
     7861 gggccgactc acccggcgcg gcgttgacag atgaggggca ggctcgattt cggccggcga
     7921 cgtggagctg gccagcctcg caaatcggcg aaaacgcctg attttacgcg agtttcccac
     7981 agatgatgtg gacaagcctg gggataagtg ccctgcggta ttgacacttg aggggcgcga
     8041 ctactgacag atgaggggcg cgatccttga cacttgaggg gcagagtgct gacagatgag
     8101 gggcgcacct attgacattt gaggggctgt ccacaggcag aaaatccagc atttgcaagg
     8161 gtttccgccc gtttttcggc caccgctaac ctgtctttta acctgctttt aaaccaatat
     8221 ttataaacct tgtttttaac cagggctgcg ccctgtgcgc gtgaccgcgc acgccgaagg
     8281 ggggtgcccc cccttctcga accctcccgg cccgctaacg cgggcctccc atccccccag
     8341 gggctgcgcc cctcggccgc gaacggcctc accccaaaaa tggcagcgct ggccaattcc
     8401 cgagtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     8461 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatggctaaa
     8521 atgagaatat caccggaatt gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg
     8581 gaaggaatgt ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa cctatattta
     8641 aaaatgacgg acagccggta taaagggacc acctatgatg tggaacggga aaaggacatg
     8701 atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga acggcatgat
     8761 ggctggagca atctgctcat gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa
     8821 gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag gctctttcac
     8881 tccatcgaca tatcggattg tccctatacg aatagcttag acagccgctt agccgaattg
     8941 gattacttac tgaataacga tctggccgat gtggattgcg aaaactggga agaggacact
     9001 ccatttaaag atccgcgcga gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa
     9061 cttgtctttt cccacggcga cctgggagac agcaacatct ttgtgaaaga tggcaaagta
     9121 agtggcttta ttgatcttgg gagaagcggc agggcggaca agtggtatga cattgccttc
     9181 tgcgtccggt cgctcaggga ggatatcggg gaagaacagt atgtcgagct attttttgac
     9241 ttactgggga tcaagcctga ttgggagaaa ataaaatatt atattttact ggatgaattg
     9301 ttttagctgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     9361 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     9421 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     9481 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     9541 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     9601 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     9661 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     9721 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     9781 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     9841 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     9901 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     9961 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    10021 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    10081 gcctttttac ggttcctggc agatcctaga tgtggcgcaa cgatgccggc gacaagcagg
    10141 agcgcaccga cttcttccgc atcaagtgtt ttggctctca ggccgaggcc cacggcaagt
    10201 atttgggcaa ggggtcgctg gtattcgtgc agggcaagat tcggaatacc aagtacgaga
    10261 aggacggcca gacggtctac gggaccgact tcattgccga taaggtggat tatctggaca
    10321 ccaaggcacc aggcgggtca aatcaggaat aagggcacat tgccccggcg tgagtcgggg
    10381 caatcccgca aggagggtga atgaatcgga cgtttgaccg gaaggcatac aggcaagaac
    10441 tgatcgacgc ggggttttcc gccgaggatg ccgaaaccat cgcaagccgc accgtcatgc
    10501 gtgcgccccg cgaaaccttc cagtccgtcg gctcgatggt ccagcaagct acggccaaga
    10561 tcgagcgcga cagcgtgcaa ctggctcccc ctgccctgcc cgcgccatcg gccgccgtgg
    10621 agcgttcgcg tcgtcttgaa caggaggcgg caggtttggc gaagtcgatg accatcgaca
    10681 cgcgaggaac tatgacgacc aagaagcgaa aaaccgccgg cgaggacctg gcaaaacagg
    10741 tcagcgaggc caagcaggcc gcgttgctga aacacacgaa gcagcagatc aaggaaatgc
    10801 agctttcctt gttcgatatt gcgccgtggc cggacacgat gcgagcgatg ccaaacgaca
    10861 cggcccgctc tgccctgttc accacgcgca acaagaaaat cccgcgcgag gcgctgcaaa
    10921 acaaggtcat tttccacgtc aacaaggacg tgaagatcac ctacaccggc gtcgagctgc
    10981 gggccgacga tgacgaactg gtgtggcagc aggtgttgga gtacgcgaag cgcaccccta
    11041 tcggcgagcc gatcaccttc acgttctacg agctttgcca ggacctgggc tggtcgatca
    11101 atggccggta ttacacgaag gccgaggaat gcctgtcgcg cctacaggcg acggcgatgg
    11161 gcttcacgtc cgaccgcgtt gggcacctgg aatcggtgtc gctgctgcac cgcttccgcg
    11221 tcctggaccg tggcaagaaa acgtcccgtt gccaggtcct gatcgacgag gaaatcgtcg
    11281 tgctgtttgc tggcgaccac tacacgaaat tcatatggga gaagtaccgc aagctgtcgc
    11341 cgacggcccg acggatgttc gactatttca gctcgcaccg ggagccgtac ccgctcaagc
    11401 tggaaacctt ccgcctcatg tgcggatcgg attccacccg cgtgaagaag tggcgcgagc
    11461 aggtcggcga agcctgcgaa gagttgcgag gcagcggcct ggtggaacac gcctgggtca
    11521 atgatgacct ggtgcattgc aaacgctagg gccttgtggg gtcagttccg gctgggggtt
    11581 cagcagcccc tgctcggatc tgttggaccg gacagtagtc atggttgatg ggctgcctgt
    11641 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag
    11701 gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt
    11761 tttaatgtac tggggttgaa cactct
//
