LOCUS       sequence-378112-        9691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as the 0-input state of the OR gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9691)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 9691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP759_L2pV1_OR-OCS_0I
FEATURES             Location/Qualifiers
     source          1..9691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    845..878
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(879..912)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      913..1620
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1621..1654
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(1655..1688)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          1755..1943
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2971..3145
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3533..5185
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3599..3617)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5205..5405
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    5469..5493
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5581..6291
                     /label=oriV
                     /note="incP origin of replication"
     promoter        6312..6416
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6417..7211
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7382..7970
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7871..7890
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8306..9454
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    9600..9624
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaagaagtt cctattctct agaaagtata ggaacttcat aacttcgtat agcatacatt
      901 atacgaagtt atctgcttta atgagatatg cgagaagcct atgatcgcat gatatttgct
      961 ttcaattctg ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga tccttaccgc
     1021 cggtttcggt tcattctaat gaatatatca cccgttacta tcgtattttt atgaataata
     1081 ttctccgttc aatttactga ttgtacccta ctacttatat gtacaatatt aaaatgaaaa
     1141 caatatattg tgctgaatag gtttatagcg acatctatga tagagcgcca caataacaaa
     1201 caattgcgtt ttattattac aaatccaatt ttaaaaaaag cggcagaacc ggtcaaacct
     1261 aaaagactga ttacataaat cttattcaaa tttcaaaagt gccccagggg ctagtatcta
     1321 cgacacaccg agcggcgaac taataacgct cactgaaggg aactccggtt ccccgccggc
     1381 gcgcatgggt gagattcctt gaagttgagt attggccgtc cgctctaccg aaagttacgg
     1441 gcaccattca acccggtcca gcacggcggc cgggtaaccg acttgctgcc ccgagaatta
     1501 tgcagcattt ttttggtgta tgtgggcccc aaatgaagtg caggtcaaac cttgacagtg
     1561 acgacaaatc gttgggcggg tccagggcga attttgcgac aacatgtcga ggctcagcag
     1621 gaagttccta ttctctagaa agtataggaa cttcataact tcgtatagca tacattatac
     1681 gaagttatca acaaacaaca ttacaattac tatttacaat tacaatgact tcaaaggtgt
     1741 atgatcctga gcaggtaagt ttctgcttct acctttgata tatatataat aattatcatt
     1801 aattagtagt aatataatat ttcaaatatt tttttcaaaa taaaagaatg tagtatatag
     1861 caattgcttt tctgtagttt ataagtgtgt atattttaat ttataacttt tctaatatat
     1921 gaccaaaatt tgttgatgtg cagagaaaaa gaatgataac tggtccacaa tggtgggcaa
     1981 gatgtaaaca gatgaacgtg cttgattctt tcattaatta ctacgattca gaaaagcacg
     2041 ctgagaatgc agttatcttt cttcacggaa acgctgcatc ttcatatttg tggagacatg
     2101 ttgtgcctca cattgaacca gtggctaggt gtattatccc agatcttatc ggaatgggaa
     2161 agtctggtaa atctggaaat ggttcttaca gacttttgga tcattataag tacctcacag
     2221 cttggttcga acttctcaac ttacctaaga agattatttt cgttggacac gattggggtg
     2281 cttgcttggc attccattac tcatacgaac accaagataa gatcaaagct atagttcatg
     2341 cagagagtgt tgtggatgtg atagaatctt gggatgagtg gccagatatt gaagaggata
     2401 ttgctttgat caagtctgaa gagggagaaa agatggttct cgaaaataac tttttcgtgg
     2461 aaactatgct tccttcaaag attatgagaa aattggaacc agaagagttt gctgcatatc
     2521 tcgaaccttt caaggaaaaa ggagaggtta gaaggccaac actcagttgg cctagggaaa
     2581 taccattagt taagggaggt aaacctgatg ttgtgcagat cgtgagaaat tataacgctt
     2641 accttagggc atcagatgat ttgcctaaga tgttcatcga gtcagatcca ggatttttca
     2701 gtaatgctat agttgaaggt gcaaagaaat ttccaaacac cgagttcgtt aaggtgaaag
     2761 gattgcattt ctcacaagaa gatgctcctg atgagatggg aaagtatatt aagtcttttg
     2821 ttgaaagagt gctcaaaaac gagcagggtt ctcgatctga tgcttctgaa gtaacgtcaa
     2881 caagctccca gtctgaggta tgcacagtgg aaacgccagg ttgtgttggt tcttaagctt
     2941 ctctagctag agtcgatcga caagctcgag tttctccata ataatgtgtg agtagttccc
     3001 agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata taagaaaccc
     3061 ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc
     3121 aaaatccagt actaaaatcc agatcgctgc aagaattcaa gcttggagcc aacactcgaa
     3181 tccccacccg ttggaccaaa cccggctcat taagcgtcgg ttcagattta tttcctttat
     3241 ttaaaaaaaa ggaaagggta aaaaatagaa aattggaaac agttaaagcc caaaattgta
     3301 atttaccgag aattgtaaat ttacctgaaa accctacgct atagtttcga ctataaatac
     3361 caaacttagg acctcacttc agaatcccct cgtcgctgcg tctctctccc gcaaccttcg
     3421 attttcgttt attcgcatcc atcggagaga gaaaacaatc aataagcgac ctactacaaa
     3481 caagtaagta agagaaaaac caaaagaaga agagaaacaa caagaagaag taatggagga
     3541 cgccaaaaac ataaagaaag gcccggcgcc attctatcct ctagaggatg gaaccgctgg
     3601 agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac
     3661 agatgcacat atcgaggtga acatcacgta cgcggaatac ttcgaaatgt ccgttcggtt
     3721 ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga
     3781 aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc
     3841 gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgaaca tttcgcagcc
     3901 taccgtagtg tttgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaaatt
     3961 accaataatc cagaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc
     4021 gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtacc
     4081 agagtccttt gatcgtgaca aaacaattgc actgataatg aattcctctg gatctactgg
     4141 gttacctaag ggtgtggccc ttccgcatag aactgcctgc gtcagattct cgcatgccag
     4201 agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt
     4261 ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt
     4321 cttaatgtat agatttgaag aagagctgtt tttacgatcc cttcaggatt acaaaattca
     4381 aagtgcgttg ctagtaccaa ccctattttc attcttcgcc aaaagcactc tgattgacaa
     4441 atacgattta tctaatttac acgaaattgc ttctgggggc gcacctcttt cgaaagaagt
     4501 cggggaagcg gttgcaaaac gcttccatct tccagggata cgacaaggat atgggctcac
     4561 tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg
     4621 taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg
     4681 cgttaatcag agaggcgaat tatgtgtcag aggacctatg attatgtccg gttatgtaaa
     4741 caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat
     4801 agcttactgg gacgaggacg aacacttctt catagttgac cgcttgaagt ctttaattaa
     4861 atacaaagga tatcaggtgg cccccgctga attggaatcg atattgttac aacaccccaa
     4921 catcttcgac gcgggcgtgg caggtctgcc cgacgatgac gccggtgaac ttcccgccgc
     4981 cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc
     5041 cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc
     5101 gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa
     5161 gaagggcgga aagtccaaat tgtaagcttg atcccccgtc gacagctagc tatatcatca
     5221 atttatgtat tacacataat atcgcactca gtctttcatc tacggcaatg taccagctga
     5281 tataatcagt tattgaaata tttctgaatt taaacttgca tcaataaatt tatgtttttg
     5341 cttggactat aatacctgac ttgttatttt atcaataaat atttaaacta tatttctttc
     5401 aagatcgcta ctagaggatg cacatgtgac cgagggacac gaagtgatcc gtttaaacta
     5461 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
     5521 aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgccagc
     5581 cgcctttgcg acgctcaccg ggctggttgc cctcgccgct gggctggcgg ccgtctatgg
     5641 ccctgcaaac gcgccagaaa cgccgtcgaa gccgtgtgcg agacaccgcg gccgccggcg
     5701 ttgtggatac ctcgcggaaa acttggccct cactgacaga tgaggggcgg acgttgacac
     5761 ttgaggggcc gactcacccg gcgcggcgtt gacagatgag gggcaggctc gatttcggcc
     5821 ggcgacgtgg agctggccag cctcgcaaat cggcgaaaac gcctgatttt acgcgagttt
     5881 cccacagatg atgtggacaa gcctggggat aagtgccctg cggtattgac acttgagggg
     5941 cgcgactact gacagatgag gggcgcgatc cttgacactt gaggggcaga gtgctgacag
     6001 atgaggggcg cacctattga catttgaggg gctgtccaca ggcagaaaat ccagcatttg
     6061 caagggtttc cgcccgtttt tcggccaccg ctaacctgtc ttttaacctg cttttaaacc
     6121 aatatttata aaccttgttt ttaaccaggg ctgcgccctg tgcgcgtgac cgcgcacgcc
     6181 gaaggggggt gccccccctt ctcgaaccct cccggcccgc taacgcgggc ctcccatccc
     6241 cccaggggct gcgcccctcg gccgcgaacg gcctcacccc aaaaatggca gcgctggcca
     6301 attcccgagt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6361 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgg
     6421 ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag
     6481 atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat
     6541 atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg
     6601 acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc
     6661 atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt
     6721 atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct
     6781 ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg
     6841 aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagagg
     6901 acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag
     6961 aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca
     7021 aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg
     7081 ccttctgcgt ccggtcgctc agggaggata tcggggaaga acagtatgtc gagctatttt
     7141 ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg
     7201 aattgtttta gctgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     7261 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     7321 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     7381 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     7441 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     7501 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     7561 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     7621 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     7681 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     7741 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     7801 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     7861 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     7921 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     7981 acgcggcctt tttacggttc ctggcagatc ctagatgtgg cgcaacgatg ccggcgacaa
     8041 gcaggagcgc accgacttct tccgcatcaa gtgttttggc tctcaggccg aggcccacgg
     8101 caagtatttg ggcaaggggt cgctggtatt cgtgcagggc aagattcgga ataccaagta
     8161 cgagaaggac ggccagacgg tctacgggac cgacttcatt gccgataagg tggattatct
     8221 ggacaccaag gcaccaggcg ggtcaaatca ggaataaggg cacattgccc cggcgtgagt
     8281 cggggcaatc ccgcaaggag ggtgaatgaa tcggacgttt gaccggaagg catacaggca
     8341 agaactgatc gacgcggggt tttccgccga ggatgccgaa accatcgcaa gccgcaccgt
     8401 catgcgtgcg ccccgcgaaa ccttccagtc cgtcggctcg atggtccagc aagctacggc
     8461 caagatcgag cgcgacagcg tgcaactggc tccccctgcc ctgcccgcgc catcggccgc
     8521 cgtggagcgt tcgcgtcgtc ttgaacagga ggcggcaggt ttggcgaagt cgatgaccat
     8581 cgacacgcga ggaactatga cgaccaagaa gcgaaaaacc gccggcgagg acctggcaaa
     8641 acaggtcagc gaggccaagc aggccgcgtt gctgaaacac acgaagcagc agatcaagga
     8701 aatgcagctt tccttgttcg atattgcgcc gtggccggac acgatgcgag cgatgccaaa
     8761 cgacacggcc cgctctgccc tgttcaccac gcgcaacaag aaaatcccgc gcgaggcgct
     8821 gcaaaacaag gtcattttcc acgtcaacaa ggacgtgaag atcacctaca ccggcgtcga
     8881 gctgcgggcc gacgatgacg aactggtgtg gcagcaggtg ttggagtacg cgaagcgcac
     8941 ccctatcggc gagccgatca ccttcacgtt ctacgagctt tgccaggacc tgggctggtc
     9001 gatcaatggc cggtattaca cgaaggccga ggaatgcctg tcgcgcctac aggcgacggc
     9061 gatgggcttc acgtccgacc gcgttgggca cctggaatcg gtgtcgctgc tgcaccgctt
     9121 ccgcgtcctg gaccgtggca agaaaacgtc ccgttgccag gtcctgatcg acgaggaaat
     9181 cgtcgtgctg tttgctggcg accactacac gaaattcata tgggagaagt accgcaagct
     9241 gtcgccgacg gcccgacgga tgttcgacta tttcagctcg caccgggagc cgtacccgct
     9301 caagctggaa accttccgcc tcatgtgcgg atcggattcc acccgcgtga agaagtggcg
     9361 cgagcaggtc ggcgaagcct gcgaagagtt gcgaggcagc ggcctggtgg aacacgcctg
     9421 ggtcaatgat gacctggtgc attgcaaacg ctagggcctt gtggggtcag ttccggctgg
     9481 gggttcagca gcccctgctc ggatctgttg gaccggacag tagtcatggt tgatgggctg
     9541 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt
     9601 ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg
     9661 acgtttttaa tgtactgggg ttgaacactc t
//
