LOCUS       sequence-378120-       13156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test the relative expression strength of the Act2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13156)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 13156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP721_pSP14
FEATURES             Location/Qualifiers
     source          1..13156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          929..1117
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2145..2319
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        3332..3677
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     3620..3640
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    3696..3751
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     primer_bind     complement(3819..3837)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          3837..4025
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    5629..5803
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    6683..6738
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             6740..7765
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     protein_bind    8369..8390
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8405..8410,8411..8428,8429..8435)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8443..8459
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8448..8470
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8467..8483
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8467..8483
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             8479..8802
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    8496..8552
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8553..8570)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8553..8569)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8562..8584)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8778..8797)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    8934..8958
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      9046..9756
                     /label=oriV
                     /note="incP origin of replication"
     promoter        9777..9881
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9882..10676
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10847..11435
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11336..11355
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             11771..12919
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    13065..13089
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtttattt acaagagcgt acggttcaac
      841 ccctgcctcc cctgtaaaac tccctagact taaatctttt ctttctagta tcttttcaat
      901 gacttcaaag gtgtatgatc ctgagcaggt aagtttctgc ttctaccttt gatatatata
      961 taataattat cattaattag tagtaatata atatttcaaa tatttttttc aaaataaaag
     1021 aatgtagtat atagcaattg cttttctgta gtttataagt gtgtatattt taatttataa
     1081 cttttctaat atatgaccaa aatttgttga tgtgcagaga aaaagaatga taactggtcc
     1141 acaatggtgg gcaagatgta aacagatgaa cgtgcttgat tctttcatta attactacga
     1201 ttcagaaaag cacgctgaga atgcagttat ctttcttcac ggaaacgctg catcttcata
     1261 tttgtggaga catgttgtgc ctcacattga accagtggct aggtgtatta tcccagatct
     1321 tatcggaatg ggaaagtctg gtaaatctgg aaatggttct tacagacttt tggatcatta
     1381 taagtacctc acagcttggt tcgaacttct caacttacct aagaagatta ttttcgttgg
     1441 acacgattgg ggtgcttgct tggcattcca ttactcatac gaacaccaag ataagatcaa
     1501 agctatagtt catgcagaga gtgttgtgga tgtgatagaa tcttgggatg agtggccaga
     1561 tattgaagag gatattgctt tgatcaagtc tgaagaggga gaaaagatgg ttctcgaaaa
     1621 taactttttc gtggaaacta tgcttccttc aaagattatg agaaaattgg aaccagaaga
     1681 gtttgctgca tatctcgaac ctttcaagga aaaaggagag gttagaaggc caacactcag
     1741 ttggcctagg gaaataccat tagttaaggg aggtaaacct gatgttgtgc agatcgtgag
     1801 aaattataac gcttacctta gggcatcaga tgatttgcct aagatgttta tcgagtcaga
     1861 tccaggattt ttcagtaatg ctatagttga aggtgcaaag aaatttccaa acaccgagtt
     1921 cgttaaggtg aaaggattgc atttctcaca agaagatgct cctgatgaga tgggaaagta
     1981 tattaagtct tttgttgaaa gagtgctcaa aaacgagcag ggttctcgat ctgatgcttc
     2041 tgaagtaacg tcaacaagct cccagtctga ggtatgcaca gtggaaacgc caggttgtgt
     2101 tggttcttaa gcttctctag ctagagtcga tcgacaagct cgagtttctc cataataatg
     2161 tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc atgtgttgag
     2221 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
     2281 taattcctaa aaccaaaatc cagtactaaa atccagatcg ctgcaagaat tcaagcttgg
     2341 aggaattcca atcccacaaa aatctgagct taacagcaca gttgctcctc tcagagcaga
     2401 atcgggtatt caacaccctc atatcaacta ctacgttgtg tataacggtc cacatgccgg
     2461 tatatacgat gactggggtt gtacaaaggc ggcaacaaac ggcgttcccg gagttgcaca
     2521 caagaaattt gccactatta cagaggcaag agcagcagct gacgcgtaca caacaagtca
     2581 gcaaacagac aggttgaact tcatccccaa aggagaagct caactcaagc ccaagagctt
     2641 tgctaaggcc ctaacaagcc caccaaagca aaaagcccac tggctcacgc taggaaccaa
     2701 aaggcccagc agtgatccag ccccaaaaga gatctccttt gccccggaga ttacaatgga
     2761 cgatttcctc tatctttacg atctaggaag gaagttcgaa ggtgaaggtg acgacactat
     2821 gttcaccact gataatgaga aggttagcct cttcaatttc agaaagaatg ctgacccaca
     2881 gatggttaga gaggcctacg cagcaagtct catcaagacg atctacccga gtaacaatct
     2941 ccaggagatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat tcaggactaa
     3001 ttgcatcaag aacacagaga aagacatatt tctcaagatc agaagtacta ttccagtatg
     3061 gacgattcaa ggcttgcttc ataaaccaag gcaagtaata gagattggag tctctaaaaa
     3121 ggtagttcct actgaatcta aggccatgca tggagtctaa gattcaaatc gaggatctaa
     3181 cagaactcgc cgtcaagact ggcgaacagt tcatacagag tcttttacga ctcaatgaca
     3241 agaagaaaat cttcgtcaac atggtggagc acgacactct ggtctactcc aaaaatgtca
     3301 aagatacagt ctcagaagat caaagggcta ttgagacttt tcaacaaagg ataatttcgg
     3361 gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa
     3421 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggctatc attcaagatc
     3481 tctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     3541 aagaggttcc aaccacgtct acaaagcaag tggattgatg tgacatctcc actgacgtaa
     3601 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     3661 ttcatttgga gaggacacgc tactgtattt ttacaacaat taccaacaac aacaaacaac
     3721 aaacaacatt acaattacta tttacaatta caatggagga cgccaaaaac ataaagaaag
     3781 gcccggcgcc attctatccg ctggaagatg gaaccgctgg agagcaactg cataaggtaa
     3841 gtttctgctt ctacctttga tatatatata ataattatca ttaattagta gtaatataat
     3901 atttcaaata tttttttcaa aataaaagaa tgtagtatat agcaattgct tttctgtagt
     3961 ttataagtgt gtatatttta atttataact tttctaatat atgaccaaaa tttgttgatg
     4021 tgcaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata
     4081 tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta
     4141 tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc
     4201 aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg
     4261 acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt
     4321 tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc
     4381 aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt
     4441 tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca gagtccttcg
     4501 atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag
     4561 gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt
     4621 ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt
     4681 ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata
     4741 gatttgaaga ggagctgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc
     4801 tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat
     4861 ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg
     4921 ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat
     4981 cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc
     5041 cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa
     5101 gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag
     5161 cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata gcttactggg
     5221 acgaggacga acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct
     5281 atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg
     5341 caggtgtcgc aggtctaccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt
     5401 tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa
     5461 caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta
     5521 ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa
     5581 agatcgccgt gtgagcttct ctagctagag tcgatcgaca agctcgagtt tctccataat
     5641 aatgtgtgag tagttcccag ataagggaat tagggttcct atagggtttc gctcatgtgt
     5701 tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa
     5761 tttctaattc ctaaaaccaa aatccagtac taaaatccag atcgctacta gaattcgagc
     5821 tcggaggagg tcattcatat gcttgagaag agagtcggga tagtccaaaa taaaacaaag
     5881 gtaagattac ctggtcaaaa gtgaaaacat cagttaaaag gtggtataaa gtaaaatatc
     5941 ggtaataaaa ggtggcccaa agtgaaattt actcttttct actattataa aaattgagga
     6001 tgtttttgtc ggtactttga tacgtcattt ttgtatgaat tggtttttaa gtttattcgc
     6061 ttttggaaat gcatatctgt atttgagtcg ggttttaagt tcgtttgctt ttgtaaatac
     6121 agagggattt gtataagaaa tatctttaaa aaaacccata tgctaatttg acataatttt
     6181 tgagaaaaat atatattcag gcgaattctc acaatgaaca ataataagat taaaatagct
     6241 ttcccccgtt gcagcgcatg ggtatttttt ctagtaaaaa taaaagataa acttagactc
     6301 aaaacattta caaaaacaac ccctaaagtt cctaaagccc aaagtgctat ccacgatcca
     6361 tagcaagccc agcccaaccc aacccaaccc aacccacccc agtccagcca actggacaat
     6421 agtctccaca cccccccact atcaccgtga gttgtccgca cgcaccgcac gtctcgcagc
     6481 caaaaaaaaa aaaagaaaga aaaaaaagaa aaagaaaaaa cagcaggtgg gtccgggtcg
     6541 tgggggccgg aaacgcgagg aggatcgcga gccagcgacg aggccggccc tccctccgct
     6601 tccaaagaaa cgccccccat cgccactata tacatacccc cccctctcct cccatccccc
     6661 caacccttac tgtattttta caacaattac caacaacaac aaacaacaaa caacattaca
     6721 attactattt acaattacaa tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
     6781 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
     6841 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     6901 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     6961 tccggaagtg cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg
     7021 ttcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc
     7081 ggtcgcggag gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     7141 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     7201 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     7261 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     7321 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     7381 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     7441 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     7501 ggagcttgca ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact
     7561 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     7621 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     7681 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     7741 cactcgtccg agggcaaaga aatgagcttg tttgagtatt atggcattgg aaaagctgtt
     7801 ttcttctatc atttgttctg cttgtaattt actgtgttct ttcagttttt gttttcggac
     7861 atcaaaatgc aaatggatgg ataagagtta ataaatgata tggtcctttt gttcattctc
     7921 aaattattat tatctgttgt ttttacttta atgggttgaa tttaagtaag aaaggaacta
     7981 acagtgtgat attaaggtgc aatgttagac atataaaaca gtctttcacc tctctttggt
     8041 tatgtcttga attggtttgt ttcttcactt atctgtgtaa tcaagtttac tatgagtcta
     8101 tgatcaagta attatgcaat caagttaagt acagtatagg ctttttgtgt cgacatttat
     8161 tcactttcct ttctgcaatt ttcaaatcat gtttgtagtt tttattggac ttgatgaatc
     8221 tctcttacca ccaaaataga acattttttc ttgatatacc agaatctcaa tatttgtctg
     8281 ataattattc tagctgagcg ctttactgag accgcagctg gcacgacagg tttcccgact
     8341 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     8401 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     8461 ttcacacagg aaacagctat gaccatgatt acgccaagct tgcatgcctg caggtcgact
     8521 ctagaggatc cccgggtacc gagctcgaat tcactggccg tcgttttaca acgtcgtgac
     8581 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     8641 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     8701 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     8761 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     8821 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     8881 caagctgtga cggtctcagg gacacgaagt gatccgttta aactatcagt gtttgacagg
     8941 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa
     9001 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg ccagccgcct ttgcgacgct
     9061 caccgggctg gttgccctcg ccgctgggct ggcggccgtc tatggccctg caaacgcgcc
     9121 agaaacgccg tcgaagccgt gtgcgagaca ccgcggccgc cggcgttgtg gatacctcgc
     9181 ggaaaacttg gccctcactg acagatgagg ggcggacgtt gacacttgag gggccgactc
     9241 acccggcgcg gcgttgacag atgaggggca ggctcgattt cggccggcga cgtggagctg
     9301 gccagcctcg caaatcggcg aaaacgcctg attttacgcg agtttcccac agatgatgtg
     9361 gacaagcctg gggataagtg ccctgcggta ttgacacttg aggggcgcga ctactgacag
     9421 atgaggggcg cgatccttga cacttgaggg gcagagtgct gacagatgag gggcgcacct
     9481 attgacattt gaggggctgt ccacaggcag aaaatccagc atttgcaagg gtttccgccc
     9541 gtttttcggc caccgctaac ctgtctttta acctgctttt aaaccaatat ttataaacct
     9601 tgtttttaac cagggctgcg ccctgtgcgc gtgaccgcgc acgccgaagg ggggtgcccc
     9661 cccttctcga accctcccgg cccgctaacg cgggcctccc atccccccag gggctgcgcc
     9721 cctcggccgc gaacggcctc accccaaaaa tggcagcgct ggccaattcc cgagtgcgcg
     9781 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     9841 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatggctaaa atgagaatat
     9901 caccggaatt gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt
     9961 ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg
    10021 acagccggta taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc
    10081 tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca
    10141 atctgctcat gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa
    10201 gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca
    10261 tatcggattg tccctatacg aatagcttag acagccgctt agccgaattg gattacttac
    10321 tgaataacga tctggccgat gtggattgcg aaaactggga agaggacact ccatttaaag
    10381 atccgcgcga gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt
    10441 cccacggcga cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta
    10501 ttgatcttgg gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt
    10561 cgctcaggga ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga
    10621 tcaagcctga ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagctgt
    10681 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    10741 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    10801 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    10861 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    10921 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    10981 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    11041 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    11101 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    11161 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    11221 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    11281 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    11341 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    11401 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    11461 ggttcctggc agatcctaga tgtggcgcaa cgatgccggc gacaagcagg agcgcaccga
    11521 cttcttccgc atcaagtgtt ttggctctca ggccgaggcc cacggcaagt atttgggcaa
    11581 ggggtcgctg gtattcgtgc agggcaagat tcggaatacc aagtacgaga aggacggcca
    11641 gacggtctac gggaccgact tcattgccga taaggtggat tatctggaca ccaaggcacc
    11701 aggcgggtca aatcaggaat aagggcacat tgccccggcg tgagtcgggg caatcccgca
    11761 aggagggtga atgaatcgga cgtttgaccg gaaggcatac aggcaagaac tgatcgacgc
    11821 ggggttttcc gccgaggatg ccgaaaccat cgcaagccgc accgtcatgc gtgcgccccg
    11881 cgaaaccttc cagtccgtcg gctcgatggt ccagcaagct acggccaaga tcgagcgcga
    11941 cagcgtgcaa ctggctcccc ctgccctgcc cgcgccatcg gccgccgtgg agcgttcgcg
    12001 tcgtcttgaa caggaggcgg caggtttggc gaagtcgatg accatcgaca cgcgaggaac
    12061 tatgacgacc aagaagcgaa aaaccgccgg cgaggacctg gcaaaacagg tcagcgaggc
    12121 caagcaggcc gcgttgctga aacacacgaa gcagcagatc aaggaaatgc agctttcctt
    12181 gttcgatatt gcgccgtggc cggacacgat gcgagcgatg ccaaacgaca cggcccgctc
    12241 tgccctgttc accacgcgca acaagaaaat cccgcgcgag gcgctgcaaa acaaggtcat
    12301 tttccacgtc aacaaggacg tgaagatcac ctacaccggc gtcgagctgc gggccgacga
    12361 tgacgaactg gtgtggcagc aggtgttgga gtacgcgaag cgcaccccta tcggcgagcc
    12421 gatcaccttc acgttctacg agctttgcca ggacctgggc tggtcgatca atggccggta
    12481 ttacacgaag gccgaggaat gcctgtcgcg cctacaggcg acggcgatgg gcttcacgtc
    12541 cgaccgcgtt gggcacctgg aatcggtgtc gctgctgcac cgcttccgcg tcctggaccg
    12601 tggcaagaaa acgtcccgtt gccaggtcct gatcgacgag gaaatcgtcg tgctgtttgc
    12661 tggcgaccac tacacgaaat tcatatggga gaagtaccgc aagctgtcgc cgacggcccg
    12721 acggatgttc gactatttca gctcgcaccg ggagccgtac ccgctcaagc tggaaacctt
    12781 ccgcctcatg tgcggatcgg attccacccg cgtgaagaag tggcgcgagc aggtcggcga
    12841 agcctgcgaa gagttgcgag gcagcggcct ggtggaacac gcctgggtca atgatgacct
    12901 ggtgcattgc aaacgctagg gccttgtggg gtcagttccg gctgggggtt cagcagcccc
    12961 tgctcggatc tgttggaccg gacagtagtc atggttgatg ggctgcctgt atcgagtggt
    13021 gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt
    13081 ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac
    13141 tggggttgaa cactct
//
