LOCUS       sequence-378123-       12777 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test the relative expression strength of the 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12777)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 12777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP720_pSP13
FEATURES             Location/Qualifiers
     source          1..12777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        99..444
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    463..518
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     intron          550..738
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    1766..1940
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        2953..3298
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    3317..3372
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     primer_bind     complement(3440..3458)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          3458..3646
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    5250..5424
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    6304..6359
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             6361..7386
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     protein_bind    7990..8011
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8026..8031,8032..8049,8050..8056)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8064..8080
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8069..8091
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8088..8104
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8088..8104
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             8100..8423
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    8117..8173
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8174..8191)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8174..8190)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8183..8205)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8399..8418)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    8555..8579
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8667..9377
                     /label=oriV
                     /note="incP origin of replication"
     promoter        9398..9502
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9503..10297
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10468..11056
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10957..10976
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             11392..12540
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    12686..12710
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga ggtcaacatg gtggagcacg acactctggt ctactccaaa
       61 aatgtcaaag atacagtctc agaagatcaa agggctattg agacttttca acaaaggata
      121 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
      181 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
      241 caagatctct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
      301 gaaaaagaag aggttccaac cacgtctaca aagcaagtgg attgatgtga catctccact
      361 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
      421 agttcatttc atttggagag gacacgctac tgtattttta caacaattac caacaacaac
      481 aaacaacaaa caacattaca attactattt acaattacaa tgacttcaaa ggtgtatgat
      541 cctgagcagg taagtttctg cttctacctt tgatatatat ataataatta tcattaatta
      601 gtagtaatat aatatttcaa atattttttt caaaataaaa gaatgtagta tatagcaatt
      661 gcttttctgt agtttataag tgtgtatatt ttaatttata acttttctaa tatatgacca
      721 aaatttgttg atgtgcagag aaaaagaatg ataactggtc cacaatggtg ggcaagatgt
      781 aaacagatga acgtgcttga ttctttcatt aattactacg attcagaaaa gcacgctgag
      841 aatgcagtta tctttcttca cggaaacgct gcatcttcat atttgtggag acatgttgtg
      901 cctcacattg aaccagtggc taggtgtatt atcccagatc ttatcggaat gggaaagtct
      961 ggtaaatctg gaaatggttc ttacagactt ttggatcatt ataagtacct cacagcttgg
     1021 ttcgaacttc tcaacttacc taagaagatt attttcgttg gacacgattg gggtgcttgc
     1081 ttggcattcc attactcata cgaacaccaa gataagatca aagctatagt tcatgcagag
     1141 agtgttgtgg atgtgataga atcttgggat gagtggccag atattgaaga ggatattgct
     1201 ttgatcaagt ctgaagaggg agaaaagatg gttctcgaaa ataacttttt cgtggaaact
     1261 atgcttcctt caaagattat gagaaaattg gaaccagaag agtttgctgc atatctcgaa
     1321 cctttcaagg aaaaaggaga ggttagaagg ccaacactca gttggcctag ggaaatacca
     1381 ttagttaagg gaggtaaacc tgatgttgtg cagatcgtga gaaattataa cgcttacctt
     1441 agggcatcag atgatttgcc taagatgttt atcgagtcag atccaggatt tttcagtaat
     1501 gctatagttg aaggtgcaaa gaaatttcca aacaccgagt tcgttaaggt gaaaggattg
     1561 catttctcac aagaagatgc tcctgatgag atgggaaagt atattaagtc ttttgttgaa
     1621 agagtgctca aaaacgagca gggttctcga tctgatgctt ctgaagtaac gtcaacaagc
     1681 tcccagtctg aggtatgcac agtggaaacg ccaggttgtg ttggttctta agcttctcta
     1741 gctagagtcg atcgacaagc tcgagtttct ccataataat gtgtgagtag ttcccagata
     1801 agggaattag ggttcctata gggtttcgct catgtgttga gcatataaga aacccttagt
     1861 atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat
     1921 ccagtactaa aatccagatc gctgcaagaa ttcaagcttg gaggaattcc aatcccacaa
     1981 aaatctgagc ttaacagcac agttgctcct ctcagagcag aatcgggtat tcaacaccct
     2041 catatcaact actacgttgt gtataacggt ccacatgccg gtatatacga tgactggggt
     2101 tgtacaaagg cggcaacaaa cggcgttccc ggagttgcac acaagaaatt tgccactatt
     2161 acagaggcaa gagcagcagc tgacgcgtac acaacaagtc agcaaacaga caggttgaac
     2221 ttcatcccca aaggagaagc tcaactcaag cccaagagct ttgctaaggc cctaacaagc
     2281 ccaccaaagc aaaaagccca ctggctcacg ctaggaacca aaaggcccag cagtgatcca
     2341 gccccaaaag agatctcctt tgccccggag attacaatgg acgatttcct ctatctttac
     2401 gatctaggaa ggaagttcga aggtgaaggt gacgacacta tgttcaccac tgataatgag
     2461 aaggttagcc tcttcaattt cagaaagaat gctgacccac agatggttag agaggcctac
     2521 gcagcaagtc tcatcaagac gatctacccg agtaacaatc tccaggagat caaatacctt
     2581 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta attgcatcaa gaacacagag
     2641 aaagacatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     2701 cataaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc tactgaatct
     2761 aaggccatgc atggagtcta agattcaaat cgaggatcta acagaactcg ccgtcaagac
     2821 tggcgaacag ttcatacaga gtcttttacg actcaatgac aagaagaaaa tcttcgtcaa
     2881 catggtggag cacgacactc tggtctactc caaaaatgtc aaagatacag tctcagaaga
     2941 tcaaagggct attgagactt ttcaacaaag gataatttcg ggaaacctcc tcggattcca
     3001 ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaaggtg gctcctacaa
     3061 atgccatcat tgcgataaag gaaaggctat cattcaagat ctctctgccg acagtggtcc
     3121 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagaggttc caaccacgtc
     3181 tacaaagcaa gtggattgat gtgacatctc cactgacgta agggatgacg cacaatccca
     3241 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agaggacacg
     3301 ctactgtatt tttacaacaa ttaccaacaa caacaaacaa caaacaacat tacaattact
     3361 atttacaatt acaatggagg acgccaaaaa cataaagaaa ggcccggcgc cattctatcc
     3421 gctggaagat ggaaccgctg gagagcaact gcataaggta agtttctgct tctacctttg
     3481 atatatatat aataattatc attaattagt agtaatataa tatttcaaat atttttttca
     3541 aaataaaaga atgtagtata tagcaattgc ttttctgtag tttataagtg tgtatatttt
     3601 aatttataac ttttctaata tatgaccaaa atttgttgat gtgcaggcta tgaagagata
     3661 cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta
     3721 cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa
     3781 tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt
     3841 gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga
     3901 attgctcaac agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt
     3961 gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga
     4021 ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc
     4081 tcccggtttt aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc
     4141 actgatcatg aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag
     4201 aactgcctgc gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc
     4261 ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact
     4321 cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag aggagctgtt
     4381 tctgaggagc cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc
     4441 cttcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc
     4501 ttctggtggc gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct
     4561 gccaggtatc aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc
     4621 cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt
     4681 tgtggatctg gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag
     4741 aggtcctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga
     4801 caaggatgga tggctacatt ctggagacat agcttactgg gacgaggacg aacacttctt
     4861 catcgttgac cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga
     4921 attggaatcc atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtctacc
     4981 cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat
     5041 gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg
     5101 cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag
     5161 aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagatcgccg tgtgagcttc
     5221 tctagctaga gtcgatcgac aagctcgagt ttctccataa taatgtgtga gtagttccca
     5281 gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat aagaaaccct
     5341 tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca
     5401 aaatccagta ctaaaatcca gatcgctact agaattcgag ctcggaggag gtcattcata
     5461 tgcttgagaa gagagtcggg atagtccaaa ataaaacaaa ggtaagatta cctggtcaaa
     5521 agtgaaaaca tcagttaaaa ggtggtataa agtaaaatat cggtaataaa aggtggccca
     5581 aagtgaaatt tactcttttc tactattata aaaattgagg atgtttttgt cggtactttg
     5641 atacgtcatt tttgtatgaa ttggttttta agtttattcg cttttggaaa tgcatatctg
     5701 tatttgagtc gggttttaag ttcgtttgct tttgtaaata cagagggatt tgtataagaa
     5761 atatctttaa aaaaacccat atgctaattt gacataattt ttgagaaaaa tatatattca
     5821 ggcgaattct cacaatgaac aataataaga ttaaaatagc tttcccccgt tgcagcgcat
     5881 gggtattttt tctagtaaaa ataaaagata aacttagact caaaacattt acaaaaacaa
     5941 cccctaaagt tcctaaagcc caaagtgcta tccacgatcc atagcaagcc cagcccaacc
     6001 caacccaacc caacccaccc cagtccagcc aactggacaa tagtctccac acccccccac
     6061 tatcaccgtg agttgtccgc acgcaccgca cgtctcgcag ccaaaaaaaa aaaaagaaag
     6121 aaaaaaaaga aaaagaaaaa acagcaggtg ggtccgggtc gtgggggccg gaaacgcgag
     6181 gaggatcgcg agccagcgac gaggccggcc ctccctccgc ttccaaagaa acgcccccca
     6241 tcgccactat atacataccc ccccctctcc tcccatcccc ccaaccctta ctgtattttt
     6301 acaacaatta ccaacaacaa caaacaacaa acaacattac aattactatt tacaattaca
     6361 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     6421 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     6481 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     6541 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     6601 ggggagttta gcgagagcct gacctattgc atctcccgcc gttcacaggg tgtcacgttg
     6661 caagacctgc ctgaaaccga actgcccgct gttctacaac cggtcgcgga ggctatggat
     6721 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     6781 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     6841 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     6901 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     6961 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     7021 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     7081 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgcca
     7141 cgactccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     7201 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     7261 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     7321 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     7381 aaatgagctt gtttgagtat tatggcattg gaaaagctgt tttcttctat catttgttct
     7441 gcttgtaatt tactgtgttc tttcagtttt tgttttcgga catcaaaatg caaatggatg
     7501 gataagagtt aataaatgat atggtccttt tgttcattct caaattatta ttatctgttg
     7561 tttttacttt aatgggttga atttaagtaa gaaaggaact aacagtgtga tattaaggtg
     7621 caatgttaga catataaaac agtctttcac ctctctttgg ttatgtcttg aattggtttg
     7681 tttcttcact tatctgtgta atcaagttta ctatgagtct atgatcaagt aattatgcaa
     7741 tcaagttaag tacagtatag gctttttgtg tcgacattta ttcactttcc tttctgcaat
     7801 tttcaaatca tgtttgtagt ttttattgga cttgatgaat ctctcttacc accaaaatag
     7861 aacatttttt cttgatatac cagaatctca atatttgtct gataattatt ctagctgagc
     7921 gctttactga gaccgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     7981 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     8041 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     8101 tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac
     8161 cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     8221 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     8281 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
     8341 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     8401 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     8461 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg acggtctcag
     8521 ggacacgaag tgatccgttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
     8581 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
     8641 ccgttcgtcc atttgtatgt gccagccgcc tttgcgacgc tcaccgggct ggttgccctc
     8701 gccgctgggc tggcggccgt ctatggccct gcaaacgcgc cagaaacgcc gtcgaagccg
     8761 tgtgcgagac accgcggccg ccggcgttgt ggatacctcg cggaaaactt ggccctcact
     8821 gacagatgag gggcggacgt tgacacttga ggggccgact cacccggcgc ggcgttgaca
     8881 gatgaggggc aggctcgatt tcggccggcg acgtggagct ggccagcctc gcaaatcggc
     8941 gaaaacgcct gattttacgc gagtttccca cagatgatgt ggacaagcct ggggataagt
     9001 gccctgcggt attgacactt gaggggcgcg actactgaca gatgaggggc gcgatccttg
     9061 acacttgagg ggcagagtgc tgacagatga ggggcgcacc tattgacatt tgaggggctg
     9121 tccacaggca gaaaatccag catttgcaag ggtttccgcc cgtttttcgg ccaccgctaa
     9181 cctgtctttt aacctgcttt taaaccaata tttataaacc ttgtttttaa ccagggctgc
     9241 gccctgtgcg cgtgaccgcg cacgccgaag gggggtgccc ccccttctcg aaccctcccg
     9301 gcccgctaac gcgggcctcc catcccccca ggggctgcgc ccctcggccg cgaacggcct
     9361 caccccaaaa atggcagcgc tggccaattc ccgagtgcgc ggaaccccta tttgtttatt
     9421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     9481 ataatattga aaaaggaaga gtatggctaa aatgagaata tcaccggaat tgaaaaaact
     9541 gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa
     9601 gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac
     9661 cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt
     9721 tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc
     9781 cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga
     9841 gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac
     9901 gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga
     9961 tgtggattgc gaaaactggg aagaggacac tccatttaaa gatccgcgcg agctgtatga
    10021 ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga
    10081 cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg
    10141 cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgctcaggg aggatatcgg
    10201 ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa
    10261 aataaaatat tatattttac tggatgaatt gttttagctg tcagaccaag tttactcata
    10321 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
    10381 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    10441 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    10501 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    10561 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
    10621 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    10681 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    10741 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    10801 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    10861 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    10921 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    10981 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    11041 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg cagatcctag
    11101 atgtggcgca acgatgccgg cgacaagcag gagcgcaccg acttcttccg catcaagtgt
    11161 tttggctctc aggccgaggc ccacggcaag tatttgggca aggggtcgct ggtattcgtg
    11221 cagggcaaga ttcggaatac caagtacgag aaggacggcc agacggtcta cgggaccgac
    11281 ttcattgccg ataaggtgga ttatctggac accaaggcac caggcgggtc aaatcaggaa
    11341 taagggcaca ttgccccggc gtgagtcggg gcaatcccgc aaggagggtg aatgaatcgg
    11401 acgtttgacc ggaaggcata caggcaagaa ctgatcgacg cggggttttc cgccgaggat
    11461 gccgaaacca tcgcaagccg caccgtcatg cgtgcgcccc gcgaaacctt ccagtccgtc
    11521 ggctcgatgg tccagcaagc tacggccaag atcgagcgcg acagcgtgca actggctccc
    11581 cctgccctgc ccgcgccatc ggccgccgtg gagcgttcgc gtcgtcttga acaggaggcg
    11641 gcaggtttgg cgaagtcgat gaccatcgac acgcgaggaa ctatgacgac caagaagcga
    11701 aaaaccgccg gcgaggacct ggcaaaacag gtcagcgagg ccaagcaggc cgcgttgctg
    11761 aaacacacga agcagcagat caaggaaatg cagctttcct tgttcgatat tgcgccgtgg
    11821 ccggacacga tgcgagcgat gccaaacgac acggcccgct ctgccctgtt caccacgcgc
    11881 aacaagaaaa tcccgcgcga ggcgctgcaa aacaaggtca ttttccacgt caacaaggac
    11941 gtgaagatca cctacaccgg cgtcgagctg cgggccgacg atgacgaact ggtgtggcag
    12001 caggtgttgg agtacgcgaa gcgcacccct atcggcgagc cgatcacctt cacgttctac
    12061 gagctttgcc aggacctggg ctggtcgatc aatggccggt attacacgaa ggccgaggaa
    12121 tgcctgtcgc gcctacaggc gacggcgatg ggcttcacgt ccgaccgcgt tgggcacctg
    12181 gaatcggtgt cgctgctgca ccgcttccgc gtcctggacc gtggcaagaa aacgtcccgt
    12241 tgccaggtcc tgatcgacga ggaaatcgtc gtgctgtttg ctggcgacca ctacacgaaa
    12301 ttcatatggg agaagtaccg caagctgtcg ccgacggccc gacggatgtt cgactatttc
    12361 agctcgcacc gggagccgta cccgctcaag ctggaaacct tccgcctcat gtgcggatcg
    12421 gattccaccc gcgtgaagaa gtggcgcgag caggtcggcg aagcctgcga agagttgcga
    12481 ggcagcggcc tggtggaaca cgcctgggtc aatgatgacc tggtgcattg caaacgctag
    12541 ggccttgtgg ggtcagttcc ggctgggggt tcagcagccc ctgctcggat ctgttggacc
    12601 ggacagtagt catggttgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
    12661 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
    12721 ttagacaact taataacaca ttgcggacgt ttttaatgta ctggggttga acactct
//
