LOCUS       sequence-378133-       12719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test for if B3 is able to de-repress a circuit with FRT, to rule 
            out cross-reactivity between these recombinases..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12719)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 12719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP742_L2pV1_CR_FRT-Rluc_TCTP-B3o_lacZ
FEATURES             Location/Qualifiers
     source          1..12719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    845..878
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      879..1586
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1587..1620
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          1687..1875
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2903..3077
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3465..5117
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3531..3549)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5137..5337
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    5680..5735
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5740..5760
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6538..6726
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    7688..7862
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    7932..7953
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7968..7973,7974..7991,7992..7998)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8006..8022
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8011..8033
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8030..8046
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8030..8046
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             8042..8365
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    8059..8115
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8116..8133)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8116..8132)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8125..8147)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8341..8360)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    8497..8521
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8609..9319
                     /label=oriV
                     /note="incP origin of replication"
     promoter        9340..9444
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9445..10239
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10410..10998
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10899..10918
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             11334..12482
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    12628..12652
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaagaagtt cctattctct agaaagtata ggaacttcct gctttaatga gatatgcgag
      901 aagcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga
      961 gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg
     1021 ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt accctactac
     1081 ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt atagcgacat
     1141 ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat ccaattttaa
     1201 aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta ttcaaatttc
     1261 aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat aacgctcact
     1321 gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag ttgagtattg
     1381 gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac ggcggccggg
     1441 taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg ggccccaaat
     1501 gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca gggcgaattt
     1561 tgcgacaaca tgtcgaggct cagcaggaag ttcctattct ctagaaagta taggaacttc
     1621 caacaaacaa cattacaatt actatttaca attacaatga cttcaaaggt gtatgatcct
     1681 gagcaggtaa gtttctgctt ctacctttga tatatatata ataattatca ttaattagta
     1741 gtaatataat atttcaaata tttttttcaa aataaaagaa tgtagtatat agcaattgct
     1801 tttctgtagt ttataagtgt gtatatttta atttataact tttctaatat atgaccaaaa
     1861 tttgttgatg tgcagagaaa aagaatgata actggtccac aatggtgggc aagatgtaaa
     1921 cagatgaacg tgcttgattc tttcattaat tactacgatt cagaaaagca cgctgagaat
     1981 gcagttatct ttcttcacgg aaacgctgca tcttcatatt tgtggagaca tgttgtgcct
     2041 cacattgaac cagtggctag gtgtattatc ccagatctta tcggaatggg aaagtctggt
     2101 aaatctggaa atggttctta cagacttttg gatcattata agtacctcac agcttggttc
     2161 gaacttctca acttacctaa gaagattatt ttcgttggac acgattgggg tgcttgcttg
     2221 gcattccatt actcatacga acaccaagat aagatcaaag ctatagttca tgcagagagt
     2281 gttgtggatg tgatagaatc ttgggatgag tggccagata ttgaagagga tattgctttg
     2341 atcaagtctg aagagggaga aaagatggtt ctcgaaaata actttttcgt ggaaactatg
     2401 cttccttcaa agattatgag aaaattggaa ccagaagagt ttgctgcata tctcgaacct
     2461 ttcaaggaaa aaggagaggt tagaaggcca acactcagtt ggcctaggga aataccatta
     2521 gttaagggag gtaaacctga tgttgtgcag atcgtgagaa attataacgc ttaccttagg
     2581 gcatcagatg atttgcctaa gatgttcatc gagtcagatc caggattttt cagtaatgct
     2641 atagttgaag gtgcaaagaa atttccaaac accgagttcg ttaaggtgaa aggattgcat
     2701 ttctcacaag aagatgctcc tgatgagatg ggaaagtata ttaagtcttt tgttgaaaga
     2761 gtgctcaaaa acgagcaggg ttctcgatct gatgcttctg aagtaacgtc aacaagctcc
     2821 cagtctgagg tatgcacagt ggaaacgcca ggttgtgttg gttcttaagc ttctctagct
     2881 agagtcgatc gacaagctcg agtttctcca taataatgtg tgagtagttc ccagataagg
     2941 gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     3001 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     3061 gtactaaaat ccagatcgct gcaagaattc aagcttggag ccaacactcg aatccccacc
     3121 cgttggacca aacccggctc attaagcgtc ggttcagatt tatttccttt atttaaaaaa
     3181 aaggaaaggg taaaaaatag aaaattggaa acagttaaag cccaaaattg taatttaccg
     3241 agaattgtaa atttacctga aaaccctacg ctatagtttc gactataaat accaaactta
     3301 ggacctcact tcagaatccc ctcgtcgctg cgtctctctc ccgcaacctt cgattttcgt
     3361 ttattcgcat ccatcggaga gagaaaacaa tcaataagcg acctactaca aacaagtaag
     3421 taagagaaaa accaaaagaa gaagagaaac aacaagaaga agtaatggag gacgccaaaa
     3481 acataaagaa aggcccggcg ccattctatc ctctagagga tggaaccgct ggagagcaac
     3541 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
     3601 atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag
     3661 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
     3721 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
     3781 acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag
     3841 tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa
     3901 tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
     3961 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct
     4021 ttgatcgtga caaaacaatt gcactgataa tgaattcctc tggatctact gggttaccta
     4081 agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta
     4141 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg
     4201 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt
     4261 atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt caaagtgcgt
     4321 tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac aaatacgatt
     4381 tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag
     4441 cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc actgagacta
     4501 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg
     4561 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc
     4621 agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta aacaatccgg
     4681 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact
     4741 gggacgagga cgaacacttc ttcatagttg accgcttgaa gtctttaatt aaatacaaag
     4801 gatatcaggt ggcccccgct gaattggaat cgatattgtt acaacacccc aacatcttcg
     4861 acgcgggcgt ggcaggtctg cccgacgatg acgccggtga acttcccgcc gccgttgttg
     4921 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag
     4981 taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc
     5041 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg
     5101 gaaagtccaa attgtaagct tgatcccccg tcgacagcta gctatatcat caatttatgt
     5161 attacacata atatcgcact cagtctttca tctacggcaa tgtaccagct gatataatca
     5221 gttattgaaa tatttctgaa tttaaacttg catcaataaa tttatgtttt tgcttggact
     5281 ataatacctg acttgttatt ttatcaataa atatttaaac tatatttctt tcaagatcgc
     5341 tactagaatt cgagctcgga gccaacactc gaatccccac ccgttggacc aaacccggct
     5401 cattaagcgt cggttcagat ttatttcctt tatttaaaaa aaaggaaagg gtaaaaaata
     5461 gaaaattgga aacagttaaa gcccaaaatt gtaatttacc gagaattgta aatttacctg
     5521 aaaaccctac gctatagttt cgactataaa taccaaactt aggacctcac ttcagaatcc
     5581 cctcgtcgct gcgtctctct cccgcaacct tcgattttcg tttattcgca tccatcggag
     5641 agagaaaaca atcaataagc gacctactgt atttttacaa caattaccaa caacaacaaa
     5701 caacaaacaa cattacaatt actatttaca attacaatgc ccaagaaaaa gcggaaggtg
     5761 tccagctaca tggacctggt ggacgacgag cccgccaccc tgtaccacaa gttcgtggaa
     5821 tgcctgaagg ccggcgagaa cttctgcggc gataagctga gcggcatcat caccatggcc
     5881 attctgaagg ccatcaaggc cctgaccgaa gtgaagaaaa ccaccttcaa caagtacaag
     5941 accaccatca agcagggcct gcagtacgac gtgggcagca gcaccatcag cttcgtgtac
     6001 cacctgaagg actgcgacga gctgagcaga ggcctgagcg acgccttcga gccctacaag
     6061 ttcaagatca agagcaacaa agaggccacc agcttcaaga ccctgttcag gggccctagc
     6121 ttcggcagcc agaagaactg gcggaagaaa gaggtggacc gcgaggtgga caacctgttc
     6181 cacagcaccg agacagacga gagcatcttc aagttcatcc tgaacaccct ggacagcatc
     6241 gaaacccaga ccaacaccga ccggcagaaa accgtgctga cctttatcct gctgatgacc
     6301 ttcttcaact gctgccggaa caacgacctg atgaacgtgg accccagcac cttcaagatc
     6361 gtgaagaaca agtttgtggg ctacctgctg caggctgaag tgaagcagac caagaccaga
     6421 aagagccgga atatcttctt cttccccatc cgggaaaacc gcttcgacct gttcctggcc
     6481 ctgcacgact tcttcagaac ctgccagccc acccccaaga gcagactgag cgatcaggta
     6541 agtttctgct tctacctttg atatatatat aataattatc attaattagt agtaatataa
     6601 tatttcaaat atttttttca aaataaaaga atgtagtata tagcaattgc ttttctgtag
     6661 tttataagtg tgtatatttt aatttataac ttttctaata tatgaccaaa atttgttgat
     6721 gtgcaggtgt ccgagcagaa gtggcagctg ttccgggaca gcatggtcat cgactacaac
     6781 cggttctttc ggaagttccc cgccagcccc atcttcgcca ttaagcacgg ccccaagtcc
     6841 cacctgggcc ggcatctgat gaacagcttt ctgcacaaga acgagctgga cagctgggcc
     6901 aacagcctgg gcaattggag cagctcccag aaccagagag agagcggcgc cagactgggc
     6961 tacacacacg gcggaagaga tctgccccag cccctgtttg gcttcctggc cggatactgc
     7021 gtgcggaacg aagagggcca catcgtgggc ctgggcctgg aaaaggacat caacgatctg
     7081 ttcgacggca tcatggaccc cctgaacgag aaagaggaca ccgagatctg cgagagctac
     7141 ggcgagtggg ccaagattgt gtccaaggac gtgctgatct tcctgaagag ataccacagc
     7201 aagaacgcct gtcggagata ccagaacagc accctgtatg cccggacctt cctgaaaacc
     7261 gagagcgtga ccctgagcgg ctccaagggc agcgaggaac cttctagccc tgtgcggatc
     7321 cccatcctga gcatgggaaa ggccagcccc tccgagggaa gaaagctgag agccagcgag
     7381 cacgccaacg acgacaacga gatcgagaag atcgacagcg acagcagcca gagcgaagag
     7441 atccctatcg agatgagcga ctccgaggac gagacaaccg ccagcaacat cagcggcatc
     7501 tacctggaca tgagcaaggc caactccaac gtggtgtaca gcccccctag ccagacaggc
     7561 agagctgctg gcgccggaag aaaaagaggc gtgggaggca gacggaccgt ggaaagcaag
     7621 cggagaagag tgctggcccc catcaaccgg tgagcttctc tagctagagt cgatcgacaa
     7681 gctcgagttt ctccataata atgtgtgagt agttcccaga taagggaatt agggttccta
     7741 tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa
     7801 aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtact aaaatccaga
     7861 tcgctttact gagaccgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     7921 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     7981 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     8041 tatgaccatg attacgccaa gcttgcatgc ctgcaggtcg actctagagg atccccgggt
     8101 accgagctcg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     8161 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     8221 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     8281 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     8341 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     8401 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgacggtctc
     8461 agggacacga agtgatccgt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa
     8521 cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt
     8581 atccgttcgt ccatttgtat gtgccagccg cctttgcgac gctcaccggg ctggttgccc
     8641 tcgccgctgg gctggcggcc gtctatggcc ctgcaaacgc gccagaaacg ccgtcgaagc
     8701 cgtgtgcgag acaccgcggc cgccggcgtt gtggatacct cgcggaaaac ttggccctca
     8761 ctgacagatg aggggcggac gttgacactt gaggggccga ctcacccggc gcggcgttga
     8821 cagatgaggg gcaggctcga tttcggccgg cgacgtggag ctggccagcc tcgcaaatcg
     8881 gcgaaaacgc ctgattttac gcgagtttcc cacagatgat gtggacaagc ctggggataa
     8941 gtgccctgcg gtattgacac ttgaggggcg cgactactga cagatgaggg gcgcgatcct
     9001 tgacacttga ggggcagagt gctgacagat gaggggcgca cctattgaca tttgaggggc
     9061 tgtccacagg cagaaaatcc agcatttgca agggtttccg cccgtttttc ggccaccgct
     9121 aacctgtctt ttaacctgct tttaaaccaa tatttataaa ccttgttttt aaccagggct
     9181 gcgccctgtg cgcgtgaccg cgcacgccga aggggggtgc ccccccttct cgaaccctcc
     9241 cggcccgcta acgcgggcct cccatccccc caggggctgc gcccctcggc cgcgaacggc
     9301 ctcaccccaa aaatggcagc gctggccaat tcccgagtgc gcggaacccc tatttgttta
     9361 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     9421 caataatatt gaaaaaggaa gagtatggct aaaatgagaa tatcaccgga attgaaaaaa
     9481 ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat
     9541 aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg
     9601 accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct
     9661 gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag
     9721 gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc
     9781 gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat
     9841 acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc
     9901 gatgtggatt gcgaaaactg ggaagaggac actccattta aagatccgcg cgagctgtat
     9961 gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga
    10021 gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc
    10081 ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgctcag ggaggatatc
    10141 ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag
    10201 aaaataaaat attatatttt actggatgaa ttgttttagc tgtcagacca agtttactca
    10261 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
    10321 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
    10381 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
    10441 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
    10501 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
    10561 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
    10621 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
    10681 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
    10741 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
    10801 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
    10861 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
    10921 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    10981 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggcagatcct
    11041 agatgtggcg caacgatgcc ggcgacaagc aggagcgcac cgacttcttc cgcatcaagt
    11101 gttttggctc tcaggccgag gcccacggca agtatttggg caaggggtcg ctggtattcg
    11161 tgcagggcaa gattcggaat accaagtacg agaaggacgg ccagacggtc tacgggaccg
    11221 acttcattgc cgataaggtg gattatctgg acaccaaggc accaggcggg tcaaatcagg
    11281 aataagggca cattgccccg gcgtgagtcg gggcaatccc gcaaggaggg tgaatgaatc
    11341 ggacgtttga ccggaaggca tacaggcaag aactgatcga cgcggggttt tccgccgagg
    11401 atgccgaaac catcgcaagc cgcaccgtca tgcgtgcgcc ccgcgaaacc ttccagtccg
    11461 tcggctcgat ggtccagcaa gctacggcca agatcgagcg cgacagcgtg caactggctc
    11521 cccctgccct gcccgcgcca tcggccgccg tggagcgttc gcgtcgtctt gaacaggagg
    11581 cggcaggttt ggcgaagtcg atgaccatcg acacgcgagg aactatgacg accaagaagc
    11641 gaaaaaccgc cggcgaggac ctggcaaaac aggtcagcga ggccaagcag gccgcgttgc
    11701 tgaaacacac gaagcagcag atcaaggaaa tgcagctttc cttgttcgat attgcgccgt
    11761 ggccggacac gatgcgagcg atgccaaacg acacggcccg ctctgccctg ttcaccacgc
    11821 gcaacaagaa aatcccgcgc gaggcgctgc aaaacaaggt cattttccac gtcaacaagg
    11881 acgtgaagat cacctacacc ggcgtcgagc tgcgggccga cgatgacgaa ctggtgtggc
    11941 agcaggtgtt ggagtacgcg aagcgcaccc ctatcggcga gccgatcacc ttcacgttct
    12001 acgagctttg ccaggacctg ggctggtcga tcaatggccg gtattacacg aaggccgagg
    12061 aatgcctgtc gcgcctacag gcgacggcga tgggcttcac gtccgaccgc gttgggcacc
    12121 tggaatcggt gtcgctgctg caccgcttcc gcgtcctgga ccgtggcaag aaaacgtccc
    12181 gttgccaggt cctgatcgac gaggaaatcg tcgtgctgtt tgctggcgac cactacacga
    12241 aattcatatg ggagaagtac cgcaagctgt cgccgacggc ccgacggatg ttcgactatt
    12301 tcagctcgca ccgggagccg tacccgctca agctggaaac cttccgcctc atgtgcggat
    12361 cggattccac ccgcgtgaag aagtggcgcg agcaggtcgg cgaagcctgc gaagagttgc
    12421 gaggcagcgg cctggtggaa cacgcctggg tcaatgatga cctggtgcat tgcaaacgct
    12481 agggccttgt ggggtcagtt ccggctgggg gttcagcagc ccctgctcgg atctgttgga
    12541 ccggacagta gtcatggttg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
    12601 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
    12661 gcttagacaa cttaataaca cattgcggac gtttttaatg tactggggtt gaacactct
//
