LOCUS       sequence-37822-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Nalbant P, Hodgson L, Kraynov V, Toutchkine A, Hahn KM
  TITLE     Activation of endogenous Cdc42 visualized in living cells.
  JOURNAL   Science. 2004 Sep 10. 305(5690):1615-9.
  PUBMED    15361624
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2004 Sep 10. 305(5690):1615-9."
COMMENT     Sequence Label: pcDNA3-EGFP-Cdc42(wt)
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..589
                     /codon_start=1
                     /product="human Rho family small GTPase Cdc42"
                     /label=Cdc42
                     /translation="MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVM
                     IGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHH
                     CPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQK
                     GLKNVFDEAILAALEPPEPKKSRRCVLL"
     promoter        complement(617..635)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(618..635)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(655..672)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    661..772
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tgtacaggaa ttcatgcaga caattaagtg tgttgttgtg ggcgatggtg ctgttggtaa
       61 aacatgtctc ctgatatcct acacaacaaa caaatttcca tcggaatatg taccgactgt
      121 ttttgacaac tatgcagtca cagttatgat tggtggagaa ccatatactc ttggactttt
      181 tgatactgca gggcaagagg attatgacag attacgaccg ctgagttatc cacaaacaga
      241 tgtatttcta gtctgttttt cagtggtctc tccatcttca tttgaaaacg tgaaagaaaa
      301 gtgggtgcct gagataactc accactgtcc aaagactcct ttcttgcttg ttgggactca
      361 aattgatctc agagatgacc cctctactat tgagaaactt gccaagaaca aacagaagcc
      421 tatcactcca gagactgctg aaaagctggc ccgtgacctg aaggctgtca agtatgtgga
      481 gtgttctgca cttacacaga aaggcctaaa gaatgtattt gacgaagcaa tattggctgc
      541 cctggagcct ccagaaccga agaagagccg caggtgtgtg ctgctatgac tcgagcatgc
      601 atctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga
      661 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
      721 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgca
//
