LOCUS       sequence-378257-        9661 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test for the repressive potential of the OCS variant (truncation)
            with the FRT sites around it..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9661)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 9661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZ
FEATURES             Location/Qualifiers
     source          1..9661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    845..878
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    1053..1086
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          1153..1341
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2369..2543
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             2931..4583
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(2997..3015)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      4603..4803
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     protein_bind    4874..4895
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4910..4915,4916..4933,4934..4940)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4948..4964
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4953..4975
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4972..4988
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4972..4988
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4984..5307
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    5001..5057
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(5058..5075)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5058..5074)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5067..5089)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5283..5302)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    5439..5463
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5551..6261
                     /label=oriV
                     /note="incP origin of replication"
     promoter        6282..6386
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6387..7181
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7352..7940
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7841..7860
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8276..9424
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    9570..9594
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaagaagtt cctattctct agaaagtata ggaacttcaa aacctgagca tgtgtagctc
      901 agatccttac cgccggtttc ggttcattct aatgaatata tcacccgtta ctatcgtatt
      961 tttatgaata atattctccg ttcaatttac tgattgtacc ctactactta tatgtacaat
     1021 attaaaatga aaacaatata ttgtgctgaa tagaagttcc tattctctag aaagtatagg
     1081 aacttccaac aaacaacatt acaattacta tttacaatta caatgacttc aaaggtgtat
     1141 gatcctgagc aggtaagttt ctgcttctac ctttgatata tatataataa ttatcattaa
     1201 ttagtagtaa tataatattt caaatatttt tttcaaaata aaagaatgta gtatatagca
     1261 attgcttttc tgtagtttat aagtgtgtat attttaattt ataacttttc taatatatga
     1321 ccaaaatttg ttgatgtgca gagaaaaaga atgataactg gtccacaatg gtgggcaaga
     1381 tgtaaacaga tgaacgtgct tgattctttc attaattact acgattcaga aaagcacgct
     1441 gagaatgcag ttatctttct tcacggaaac gctgcatctt catatttgtg gagacatgtt
     1501 gtgcctcaca ttgaaccagt ggctaggtgt attatcccag atcttatcgg aatgggaaag
     1561 tctggtaaat ctggaaatgg ttcttacaga cttttggatc attataagta cctcacagct
     1621 tggttcgaac ttctcaactt acctaagaag attattttcg ttggacacga ttggggtgct
     1681 tgcttggcat tccattactc atacgaacac caagataaga tcaaagctat agttcatgca
     1741 gagagtgttg tggatgtgat agaatcttgg gatgagtggc cagatattga agaggatatt
     1801 gctttgatca agtctgaaga gggagaaaag atggttctcg aaaataactt tttcgtggaa
     1861 actatgcttc cttcaaagat tatgagaaaa ttggaaccag aagagtttgc tgcatatctc
     1921 gaacctttca aggaaaaagg agaggttaga aggccaacac tcagttggcc tagggaaata
     1981 ccattagtta agggaggtaa acctgatgtt gtgcagatcg tgagaaatta taacgcttac
     2041 cttagggcat cagatgattt gcctaagatg ttcatcgagt cagatccagg atttttcagt
     2101 aatgctatag ttgaaggtgc aaagaaattt ccaaacaccg agttcgttaa ggtgaaagga
     2161 ttgcatttct cacaagaaga tgctcctgat gagatgggaa agtatattaa gtcttttgtt
     2221 gaaagagtgc tcaaaaacga gcagggttct cgatctgatg cttctgaagt aacgtcaaca
     2281 agctcccagt ctgaggtatg cacagtggaa acgccaggtt gtgttggttc ttaagcttct
     2341 ctagctagag tcgatcgaca agctcgagtt tctccataat aatgtgtgag tagttcccag
     2401 ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata agaaaccctt
     2461 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
     2521 aatccagtac taaaatccag atcgctgcaa gaattcaagc ttggagccaa cactcgaatc
     2581 cccacccgtt ggaccaaacc cggctcatta agcgtcggtt cagatttatt tcctttattt
     2641 aaaaaaaagg aaagggtaaa aaatagaaaa ttggaaacag ttaaagccca aaattgtaat
     2701 ttaccgagaa ttgtaaattt acctgaaaac cctacgctat agtttcgact ataaatacca
     2761 aacttaggac ctcacttcag aatcccctcg tcgctgcgtc tctctcccgc aaccttcgat
     2821 tttcgtttat tcgcatccat cggagagaga aaacaatcaa taagcgacct actacaaaca
     2881 agtaagtaag agaaaaacca aaagaagaag agaaacaaca agaagaagta atggaggacg
     2941 ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga accgctggag
     3001 agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag
     3061 atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc gttcggttgg
     3121 cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa
     3181 actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc
     3241 ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt tcgcagccta
     3301 ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac
     3361 caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga
     3421 tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtaccag
     3481 agtcctttga tcgtgacaaa acaattgcac tgataatgaa ttcctctgga tctactgggt
     3541 tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg catgccagag
     3601 atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc
     3661 atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct
     3721 taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac aaaattcaaa
     3781 gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg attgacaaat
     3841 acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg aaagaagtcg
     3901 gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat gggctcactg
     3961 agactacatc agctattctg attacacccg agggggatga taaaccgggc gcggtcggta
     4021 aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg
     4081 ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt tatgtaaaca
     4141 atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag
     4201 cttactggga cgaggacgaa cacttcttca tagttgaccg cttgaagtct ttaattaaat
     4261 acaaaggata tcaggtggcc cccgctgaat tggaatcgat attgttacaa caccccaaca
     4321 tcttcgacgc gggcgtggca ggtctgcccg acgatgacgc cggtgaactt cccgccgccg
     4381 ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca
     4441 gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga
     4501 aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga
     4561 agggcggaaa gtccaaattg taagcttgat cccccgtcga cagctagcta tatcatcaat
     4621 ttatgtatta cacataatat cgcactcagt ctttcatcta cggcaatgta ccagctgata
     4681 taatcagtta ttgaaatatt tctgaattta aacttgcatc aataaattta tgtttttgct
     4741 tggactataa tacctgactt gttattttat caataaatat ttaaactata tttctttcaa
     4801 gatcgctact atgagaccgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     4861 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     4921 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     4981 gctatgacca tgattacgcc aagcttgcat gcctgcaggt cgactctaga ggatccccgg
     5041 gtaccgagct cgaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     5101 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     5161 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
     5221 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     5281 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     5341 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgacggtc
     5401 tcagggacac gaagtgatcc gtttaaacta tcagtgtttg acaggatata ttggcgggta
     5461 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
     5521 ttatccgttc gtccatttgt atgtgccagc cgcctttgcg acgctcaccg ggctggttgc
     5581 cctcgccgct gggctggcgg ccgtctatgg ccctgcaaac gcgccagaaa cgccgtcgaa
     5641 gccgtgtgcg agacaccgcg gccgccggcg ttgtggatac ctcgcggaaa acttggccct
     5701 cactgacaga tgaggggcgg acgttgacac ttgaggggcc gactcacccg gcgcggcgtt
     5761 gacagatgag gggcaggctc gatttcggcc ggcgacgtgg agctggccag cctcgcaaat
     5821 cggcgaaaac gcctgatttt acgcgagttt cccacagatg atgtggacaa gcctggggat
     5881 aagtgccctg cggtattgac acttgagggg cgcgactact gacagatgag gggcgcgatc
     5941 cttgacactt gaggggcaga gtgctgacag atgaggggcg cacctattga catttgaggg
     6001 gctgtccaca ggcagaaaat ccagcatttg caagggtttc cgcccgtttt tcggccaccg
     6061 ctaacctgtc ttttaacctg cttttaaacc aatatttata aaccttgttt ttaaccaggg
     6121 ctgcgccctg tgcgcgtgac cgcgcacgcc gaaggggggt gccccccctt ctcgaaccct
     6181 cccggcccgc taacgcgggc ctcccatccc cccaggggct gcgcccctcg gccgcgaacg
     6241 gcctcacccc aaaaatggca gcgctggcca attcccgagt gcgcggaacc cctatttgtt
     6301 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6361 ttcaataata ttgaaaaagg aagagtatgg ctaaaatgag aatatcaccg gaattgaaaa
     6421 aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct gctaaggtat
     6481 ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc cggtataaag
     6541 ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa ggaaagctgc
     6601 ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg ctcatgagtg
     6661 aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct gaaaagatta
     6721 tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg gattgtccct
     6781 atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat aacgatctgg
     6841 ccgatgtgga ttgcgaaaac tgggaagagg acactccatt taaagatccg cgcgagctgt
     6901 atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac ggcgacctgg
     6961 gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat cttgggagaa
     7021 gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgctc agggaggata
     7081 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
     7141 agaaaataaa atattatatt ttactggatg aattgtttta gctgtcagac caagtttact
     7201 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7261 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7321 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7381 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7441 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7501 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7561 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7621 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7681 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7741 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7801 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7861 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7921 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcagatc
     7981 ctagatgtgg cgcaacgatg ccggcgacaa gcaggagcgc accgacttct tccgcatcaa
     8041 gtgttttggc tctcaggccg aggcccacgg caagtatttg ggcaaggggt cgctggtatt
     8101 cgtgcagggc aagattcgga ataccaagta cgagaaggac ggccagacgg tctacgggac
     8161 cgacttcatt gccgataagg tggattatct ggacaccaag gcaccaggcg ggtcaaatca
     8221 ggaataaggg cacattgccc cggcgtgagt cggggcaatc ccgcaaggag ggtgaatgaa
     8281 tcggacgttt gaccggaagg catacaggca agaactgatc gacgcggggt tttccgccga
     8341 ggatgccgaa accatcgcaa gccgcaccgt catgcgtgcg ccccgcgaaa ccttccagtc
     8401 cgtcggctcg atggtccagc aagctacggc caagatcgag cgcgacagcg tgcaactggc
     8461 tccccctgcc ctgcccgcgc catcggccgc cgtggagcgt tcgcgtcgtc ttgaacagga
     8521 ggcggcaggt ttggcgaagt cgatgaccat cgacacgcga ggaactatga cgaccaagaa
     8581 gcgaaaaacc gccggcgagg acctggcaaa acaggtcagc gaggccaagc aggccgcgtt
     8641 gctgaaacac acgaagcagc agatcaagga aatgcagctt tccttgttcg atattgcgcc
     8701 gtggccggac acgatgcgag cgatgccaaa cgacacggcc cgctctgccc tgttcaccac
     8761 gcgcaacaag aaaatcccgc gcgaggcgct gcaaaacaag gtcattttcc acgtcaacaa
     8821 ggacgtgaag atcacctaca ccggcgtcga gctgcgggcc gacgatgacg aactggtgtg
     8881 gcagcaggtg ttggagtacg cgaagcgcac ccctatcggc gagccgatca ccttcacgtt
     8941 ctacgagctt tgccaggacc tgggctggtc gatcaatggc cggtattaca cgaaggccga
     9001 ggaatgcctg tcgcgcctac aggcgacggc gatgggcttc acgtccgacc gcgttgggca
     9061 cctggaatcg gtgtcgctgc tgcaccgctt ccgcgtcctg gaccgtggca agaaaacgtc
     9121 ccgttgccag gtcctgatcg acgaggaaat cgtcgtgctg tttgctggcg accactacac
     9181 gaaattcata tgggagaagt accgcaagct gtcgccgacg gcccgacgga tgttcgacta
     9241 tttcagctcg caccgggagc cgtacccgct caagctggaa accttccgcc tcatgtgcgg
     9301 atcggattcc acccgcgtga agaagtggcg cgagcaggtc ggcgaagcct gcgaagagtt
     9361 gcgaggcagc ggcctggtgg aacacgcctg ggtcaatgat gacctggtgc attgcaaacg
     9421 ctagggcctt gtggggtcag ttccggctgg gggttcagca gcccctgctc ggatctgttg
     9481 gaccggacag tagtcatggt tgatgggctg cctgtatcga gtggtgattt tgtgccgagc
     9541 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg
     9601 acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgggg ttgaacactc
     9661 t
//
