LOCUS       sequence-378260-        9692 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test for the repressive potential of the 35S terminator from CaMV
            with the FRT sites around it..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9692)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 9692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP732_L2_pV01_0I_FRT-35Ster-Rluc_lacZ
FEATURES             Location/Qualifiers
     source          1..9692
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    845..878
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    909..1083
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    1084..1117
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          1184..1372
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2400..2574
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             2962..4614
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3028..3046)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      4634..4834
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     protein_bind    4905..4926
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4941..4946,4947..4964,4965..4971)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4979..4995
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4984..5006
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5003..5019
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5003..5019
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5015..5338
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    5032..5088
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(5089..5106)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5089..5105)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5098..5120)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5314..5333)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    5470..5494
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5582..6292
                     /label=oriV
                     /note="incP origin of replication"
     promoter        6313..6417
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6418..7212
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7383..7971
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7872..7891
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8307..9455
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    9601..9625
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaagaagtt cctattctct agaaagtata ggaacttcct ctagctagag tcgatcgaca
      901 agctcgagtt tctccataat aatgtgtgag tagttcccag ataagggaat tagggttcct
      961 atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta
     1021 aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag
     1081 atcgaagttc ctattctcta gaaagtatag gaacttccaa caaacaacat tacaattact
     1141 atttacaatt acaatgactt caaaggtgta tgatcctgag caggtaagtt tctgcttcta
     1201 cctttgatat atatataata attatcatta attagtagta atataatatt tcaaatattt
     1261 ttttcaaaat aaaagaatgt agtatatagc aattgctttt ctgtagttta taagtgtgta
     1321 tattttaatt tataactttt ctaatatatg accaaaattt gttgatgtgc agagaaaaag
     1381 aatgataact ggtccacaat ggtgggcaag atgtaaacag atgaacgtgc ttgattcttt
     1441 cattaattac tacgattcag aaaagcacgc tgagaatgca gttatctttc ttcacggaaa
     1501 cgctgcatct tcatatttgt ggagacatgt tgtgcctcac attgaaccag tggctaggtg
     1561 tattatccca gatcttatcg gaatgggaaa gtctggtaaa tctggaaatg gttcttacag
     1621 acttttggat cattataagt acctcacagc ttggttcgaa cttctcaact tacctaagaa
     1681 gattattttc gttggacacg attggggtgc ttgcttggca ttccattact catacgaaca
     1741 ccaagataag atcaaagcta tagttcatgc agagagtgtt gtggatgtga tagaatcttg
     1801 ggatgagtgg ccagatattg aagaggatat tgctttgatc aagtctgaag agggagaaaa
     1861 gatggttctc gaaaataact ttttcgtgga aactatgctt ccttcaaaga ttatgagaaa
     1921 attggaacca gaagagtttg ctgcatatct cgaacctttc aaggaaaaag gagaggttag
     1981 aaggccaaca ctcagttggc ctagggaaat accattagtt aagggaggta aacctgatgt
     2041 tgtgcagatc gtgagaaatt ataacgctta ccttagggca tcagatgatt tgcctaagat
     2101 gttcatcgag tcagatccag gatttttcag taatgctata gttgaaggtg caaagaaatt
     2161 tccaaacacc gagttcgtta aggtgaaagg attgcatttc tcacaagaag atgctcctga
     2221 tgagatggga aagtatatta agtcttttgt tgaaagagtg ctcaaaaacg agcagggttc
     2281 tcgatctgat gcttctgaag taacgtcaac aagctcccag tctgaggtat gcacagtgga
     2341 aacgccaggt tgtgttggtt cttaagcttc tctagctaga gtcgatcgac aagctcgagt
     2401 ttctccataa taatgtgtga gtagttccca gataagggaa ttagggttcc tatagggttt
     2461 cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc
     2521 tatcaataaa atttctaatt cctaaaacca aaatccagta ctaaaatcca gatcgctgca
     2581 agaattcaag cttggagcca acactcgaat ccccacccgt tggaccaaac ccggctcatt
     2641 aagcgtcggt tcagatttat ttcctttatt taaaaaaaag gaaagggtaa aaaatagaaa
     2701 attggaaaca gttaaagccc aaaattgtaa tttaccgaga attgtaaatt tacctgaaaa
     2761 ccctacgcta tagtttcgac tataaatacc aaacttagga cctcacttca gaatcccctc
     2821 gtcgctgcgt ctctctcccg caaccttcga ttttcgttta ttcgcatcca tcggagagag
     2881 aaaacaatca ataagcgacc tactacaaac aagtaagtaa gagaaaaacc aaaagaagaa
     2941 gagaaacaac aagaagaagt aatggaggac gccaaaaaca taaagaaagg cccggcgcca
     3001 ttctatcctc tagaggatgg aaccgctgga gagcaactgc ataaggctat gaagagatac
     3061 gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgaa catcacgtac
     3121 gcggaatact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat
     3181 acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg
     3241 ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa
     3301 ttgctcaaca gtatgaacat ttcgcagcct accgtagtgt ttgtttccaa aaaggggttg
     3361 caaaaaattt tgaacgtgca aaaaaaatta ccaataatcc agaaaattat tatcatggat
     3421 tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct
     3481 cccggtttta atgaatacga ttttgtacca gagtcctttg atcgtgacaa aacaattgca
     3541 ctgataatga attcctctgg atctactggg ttacctaagg gtgtggccct tccgcataga
     3601 actgcctgcg tcagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg
     3661 gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc
     3721 ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt
     3781 ttacgatccc ttcaggatta caaaattcaa agtgcgttgc tagtaccaac cctattttca
     3841 ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct
     3901 tctgggggcg cacctctttc gaaagaagtc ggggaagcgg ttgcaaaacg cttccatctt
     3961 ccagggatac gacaaggata tgggctcact gagactacat cagctattct gattacaccc
     4021 gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt
     4081 gtggatctgg ataccgggaa aacgctgggc gttaatcaga gaggcgaatt atgtgtcaga
     4141 ggacctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac
     4201 aaggatggat ggctacattc tggagacata gcttactggg acgaggacga acacttcttc
     4261 atagttgacc gcttgaagtc tttaattaaa tacaaaggat atcaggtggc ccccgctgaa
     4321 ttggaatcga tattgttaca acaccccaac atcttcgacg cgggcgtggc aggtctgccc
     4381 gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg
     4441 acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc
     4501 ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga
     4561 aaaatcagag agatcctcat aaaggccaag aagggcggaa agtccaaatt gtaagcttga
     4621 tcccccgtcg acagctagct atatcatcaa tttatgtatt acacataata tcgcactcag
     4681 tctttcatct acggcaatgt accagctgat ataatcagtt attgaaatat ttctgaattt
     4741 aaacttgcat caataaattt atgtttttgc ttggactata atacctgact tgttatttta
     4801 tcaataaata tttaaactat atttctttca agatcgctac tatgagaccg cagctggcac
     4861 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     4921 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     4981 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca
     5041 tgcctgcagg tcgactctag aggatccccg ggtaccgagc tcgaattcac tggccgtcgt
     5101 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     5161 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     5221 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
     5281 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     5341 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     5401 ggcatccgct tacagacaag ctgtgacggt ctcagggaca cgaagtgatc cgtttaaact
     5461 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     5521 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgccag
     5581 ccgcctttgc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
     5641 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc
     5701 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
     5761 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
     5821 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
     5881 tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
     5941 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
     6001 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
     6061 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
     6121 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
     6181 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
     6241 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agcgctggcc
     6301 aattcccgag tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     6361 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     6421 gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa
     6481 gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta
     6541 tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag
     6601 gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg
     6661 catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag
     6721 tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc
     6781 tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag ccgcttagcc
     6841 gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagag
     6901 gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa
     6961 gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc
     7021 aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt
     7081 gccttctgcg tccggtcgct cagggaggat atcggggaag aacagtatgt cgagctattt
     7141 tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat
     7201 gaattgtttt agctgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7261 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7321 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7381 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7441 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7501 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     7561 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7621 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7681 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7741 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7801 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7861 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7921 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7981 aacgcggcct ttttacggtt cctggcagat cctagatgtg gcgcaacgat gccggcgaca
     8041 agcaggagcg caccgacttc ttccgcatca agtgttttgg ctctcaggcc gaggcccacg
     8101 gcaagtattt gggcaagggg tcgctggtat tcgtgcaggg caagattcgg aataccaagt
     8161 acgagaagga cggccagacg gtctacggga ccgacttcat tgccgataag gtggattatc
     8221 tggacaccaa ggcaccaggc gggtcaaatc aggaataagg gcacattgcc ccggcgtgag
     8281 tcggggcaat cccgcaagga gggtgaatga atcggacgtt tgaccggaag gcatacaggc
     8341 aagaactgat cgacgcgggg ttttccgccg aggatgccga aaccatcgca agccgcaccg
     8401 tcatgcgtgc gccccgcgaa accttccagt ccgtcggctc gatggtccag caagctacgg
     8461 ccaagatcga gcgcgacagc gtgcaactgg ctccccctgc cctgcccgcg ccatcggccg
     8521 ccgtggagcg ttcgcgtcgt cttgaacagg aggcggcagg tttggcgaag tcgatgacca
     8581 tcgacacgcg aggaactatg acgaccaaga agcgaaaaac cgccggcgag gacctggcaa
     8641 aacaggtcag cgaggccaag caggccgcgt tgctgaaaca cacgaagcag cagatcaagg
     8701 aaatgcagct ttccttgttc gatattgcgc cgtggccgga cacgatgcga gcgatgccaa
     8761 acgacacggc ccgctctgcc ctgttcacca cgcgcaacaa gaaaatcccg cgcgaggcgc
     8821 tgcaaaacaa ggtcattttc cacgtcaaca aggacgtgaa gatcacctac accggcgtcg
     8881 agctgcgggc cgacgatgac gaactggtgt ggcagcaggt gttggagtac gcgaagcgca
     8941 cccctatcgg cgagccgatc accttcacgt tctacgagct ttgccaggac ctgggctggt
     9001 cgatcaatgg ccggtattac acgaaggccg aggaatgcct gtcgcgccta caggcgacgg
     9061 cgatgggctt cacgtccgac cgcgttgggc acctggaatc ggtgtcgctg ctgcaccgct
     9121 tccgcgtcct ggaccgtggc aagaaaacgt cccgttgcca ggtcctgatc gacgaggaaa
     9181 tcgtcgtgct gtttgctggc gaccactaca cgaaattcat atgggagaag taccgcaagc
     9241 tgtcgccgac ggcccgacgg atgttcgact atttcagctc gcaccgggag ccgtacccgc
     9301 tcaagctgga aaccttccgc ctcatgtgcg gatcggattc cacccgcgtg aagaagtggc
     9361 gcgagcaggt cggcgaagcc tgcgaagagt tgcgaggcag cggcctggtg gaacacgcct
     9421 gggtcaatga tgacctggtg cattgcaaac gctagggcct tgtggggtca gttccggctg
     9481 ggggttcagc agcccctgct cggatctgtt ggaccggaca gtagtcatgg ttgatgggct
     9541 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
     9601 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
     9661 gacgttttta atgtactggg gttgaacact ct
//
