LOCUS       sequence-378273-       14880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as a 2-input state of the OR gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14880)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 14880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP747_L2pV1_OR_B3RT_FRT_Rluc_2IBF_lacZ
FEATURES             Location/Qualifiers
     source          1..14880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    845..878
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      912..1619
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1620..1653
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          1753..1941
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2969..3143
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3531..5183
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3597..3615)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5203..5403
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    5746..5801
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5806..5826
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6604..6792
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    7754..7928
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    8270..8325
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             8333..8353
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          9037..9225
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    9849..10023
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    10093..10114
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10129..10134,10135..10152,10153..10159)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10167..10183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10172..10194
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10191..10207
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10191..10207
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             10203..10526
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    10220..10276
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(10277..10294)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10277..10293)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10286..10308)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10502..10521)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    10658..10682
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      10770..11480
                     /label=oriV
                     /note="incP origin of replication"
     promoter        11501..11605
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11606..12400
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12571..13159
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13060..13079
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             13495..14643
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    14789..14813
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaagaagtt cctattctct agaaagtata ggaacttcgg ttgcttaaga ataagtaatt
      901 cttaagcaac cctgctttaa tgagatatgc gagaagccta tgatcgcatg atatttgctt
      961 tcaattctgt tgtgcacgtt gtaaaaaacc tgagcatgtg tagctcagat ccttaccgcc
     1021 ggtttcggtt cattctaatg aatatatcac ccgttactat cgtattttta tgaataatat
     1081 tctccgttca atttactgat tgtaccctac tacttatatg tacaatatta aaatgaaaac
     1141 aatatattgt gctgaatagg tttatagcga catctatgat agagcgccac aataacaaac
     1201 aattgcgttt tattattaca aatccaattt taaaaaaagc ggcagaaccg gtcaaaccta
     1261 aaagactgat tacataaatc ttattcaaat ttcaaaagtg ccccaggggc tagtatctac
     1321 gacacaccga gcggcgaact aataacgctc actgaaggga actccggttc cccgccggcg
     1381 cgcatgggtg agattccttg aagttgagta ttggccgtcc gctctaccga aagttacggg
     1441 caccattcaa cccggtccag cacggcggcc gggtaaccga cttgctgccc cgagaattat
     1501 gcagcatttt tttggtgtat gtgggcccca aatgaagtgc aggtcaaacc ttgacagtga
     1561 cgacaaatcg ttgggcgggt ccagggcgaa ttttgcgaca acatgtcgag gctcagcagg
     1621 aagttcctat tctctagaaa gtataggaac ttcggttgct taagaataag taattcttaa
     1681 gcaacccaac aaacaacatt acaattacta tttacaatta caatgacttc aaaggtgtat
     1741 gatcctgagc aggtaagttt ctgcttctac ctttgatata tatataataa ttatcattaa
     1801 ttagtagtaa tataatattt caaatatttt tttcaaaata aaagaatgta gtatatagca
     1861 attgcttttc tgtagtttat aagtgtgtat attttaattt ataacttttc taatatatga
     1921 ccaaaatttg ttgatgtgca gagaaaaaga atgataactg gtccacaatg gtgggcaaga
     1981 tgtaaacaga tgaacgtgct tgattctttc attaattact acgattcaga aaagcacgct
     2041 gagaatgcag ttatctttct tcacggaaac gctgcatctt catatttgtg gagacatgtt
     2101 gtgcctcaca ttgaaccagt ggctaggtgt attatcccag atcttatcgg aatgggaaag
     2161 tctggtaaat ctggaaatgg ttcttacaga cttttggatc attataagta cctcacagct
     2221 tggttcgaac ttctcaactt acctaagaag attattttcg ttggacacga ttggggtgct
     2281 tgcttggcat tccattactc atacgaacac caagataaga tcaaagctat agttcatgca
     2341 gagagtgttg tggatgtgat agaatcttgg gatgagtggc cagatattga agaggatatt
     2401 gctttgatca agtctgaaga gggagaaaag atggttctcg aaaataactt tttcgtggaa
     2461 actatgcttc cttcaaagat tatgagaaaa ttggaaccag aagagtttgc tgcatatctc
     2521 gaacctttca aggaaaaagg agaggttaga aggccaacac tcagttggcc tagggaaata
     2581 ccattagtta agggaggtaa acctgatgtt gtgcagatcg tgagaaatta taacgcttac
     2641 cttagggcat cagatgattt gcctaagatg ttcatcgagt cagatccagg atttttcagt
     2701 aatgctatag ttgaaggtgc aaagaaattt ccaaacaccg agttcgttaa ggtgaaagga
     2761 ttgcatttct cacaagaaga tgctcctgat gagatgggaa agtatattaa gtcttttgtt
     2821 gaaagagtgc tcaaaaacga gcagggttct cgatctgatg cttctgaagt aacgtcaaca
     2881 agctcccagt ctgaggtatg cacagtggaa acgccaggtt gtgttggttc ttaagcttct
     2941 ctagctagag tcgatcgaca agctcgagtt tctccataat aatgtgtgag tagttcccag
     3001 ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata agaaaccctt
     3061 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
     3121 aatccagtac taaaatccag atcgctgcaa gaattcaagc ttggagccaa cactcgaatc
     3181 cccacccgtt ggaccaaacc cggctcatta agcgtcggtt cagatttatt tcctttattt
     3241 aaaaaaaagg aaagggtaaa aaatagaaaa ttggaaacag ttaaagccca aaattgtaat
     3301 ttaccgagaa ttgtaaattt acctgaaaac cctacgctat agtttcgact ataaatacca
     3361 aacttaggac ctcacttcag aatcccctcg tcgctgcgtc tctctcccgc aaccttcgat
     3421 tttcgtttat tcgcatccat cggagagaga aaacaatcaa taagcgacct actacaaaca
     3481 agtaagtaag agaaaaacca aaagaagaag agaaacaaca agaagaagta atggaggacg
     3541 ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga accgctggag
     3601 agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag
     3661 atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc gttcggttgg
     3721 cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa
     3781 actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc
     3841 ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt tcgcagccta
     3901 ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac
     3961 caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga
     4021 tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtaccag
     4081 agtcctttga tcgtgacaaa acaattgcac tgataatgaa ttcctctgga tctactgggt
     4141 tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg catgccagag
     4201 atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc
     4261 atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct
     4321 taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac aaaattcaaa
     4381 gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg attgacaaat
     4441 acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg aaagaagtcg
     4501 gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat gggctcactg
     4561 agactacatc agctattctg attacacccg agggggatga taaaccgggc gcggtcggta
     4621 aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg
     4681 ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt tatgtaaaca
     4741 atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag
     4801 cttactggga cgaggacgaa cacttcttca tagttgaccg cttgaagtct ttaattaaat
     4861 acaaaggata tcaggtggcc cccgctgaat tggaatcgat attgttacaa caccccaaca
     4921 tcttcgacgc gggcgtggca ggtctgcccg acgatgacgc cggtgaactt cccgccgccg
     4981 ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca
     5041 gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga
     5101 aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga
     5161 agggcggaaa gtccaaattg taagcttgat cccccgtcga cagctagcta tatcatcaat
     5221 ttatgtatta cacataatat cgcactcagt ctttcatcta cggcaatgta ccagctgata
     5281 taatcagtta ttgaaatatt tctgaattta aacttgcatc aataaattta tgtttttgct
     5341 tggactataa tacctgactt gttattttat caataaatat ttaaactata tttctttcaa
     5401 gatcgctact agaattcgag ctcggagcca acactcgaat ccccacccgt tggaccaaac
     5461 ccggctcatt aagcgtcggt tcagatttat ttcctttatt taaaaaaaag gaaagggtaa
     5521 aaaatagaaa attggaaaca gttaaagccc aaaattgtaa tttaccgaga attgtaaatt
     5581 tacctgaaaa ccctacgcta tagtttcgac tataaatacc aaacttagga cctcacttca
     5641 gaatcccctc gtcgctgcgt ctctctcccg caaccttcga ttttcgttta ttcgcatcca
     5701 tcggagagag aaaacaatca ataagcgacc tactgtattt ttacaacaat taccaacaac
     5761 aacaaacaac aaacaacatt acaattacta tttacaatta caatgcccaa gaaaaagcgg
     5821 aaggtgtcca gctacatgga cctggtggac gacgagcccg ccaccctgta ccacaagttc
     5881 gtggaatgcc tgaaggccgg cgagaacttc tgcggcgata agctgagcgg catcatcacc
     5941 atggccattc tgaaggccat caaggccctg accgaagtga agaaaaccac cttcaacaag
     6001 tacaagacca ccatcaagca gggcctgcag tacgacgtgg gcagcagcac catcagcttc
     6061 gtgtaccacc tgaaggactg cgacgagctg agcagaggcc tgagcgacgc cttcgagccc
     6121 tacaagttca agatcaagag caacaaagag gccaccagct tcaagaccct gttcaggggc
     6181 cctagcttcg gcagccagaa gaactggcgg aagaaagagg tggaccgcga ggtggacaac
     6241 ctgttccaca gcaccgagac agacgagagc atcttcaagt tcatcctgaa caccctggac
     6301 agcatcgaaa cccagaccaa caccgaccgg cagaaaaccg tgctgacctt tatcctgctg
     6361 atgaccttct tcaactgctg ccggaacaac gacctgatga acgtggaccc cagcaccttc
     6421 aagatcgtga agaacaagtt tgtgggctac ctgctgcagg ctgaagtgaa gcagaccaag
     6481 accagaaaga gccggaatat cttcttcttc cccatccggg aaaaccgctt cgacctgttc
     6541 ctggccctgc acgacttctt cagaacctgc cagcccaccc ccaagagcag actgagcgat
     6601 caggtaagtt tctgcttcta cctttgatat atatataata attatcatta attagtagta
     6661 atataatatt tcaaatattt ttttcaaaat aaaagaatgt agtatatagc aattgctttt
     6721 ctgtagttta taagtgtgta tattttaatt tataactttt ctaatatatg accaaaattt
     6781 gttgatgtgc aggtgtccga gcagaagtgg cagctgttcc gggacagcat ggtcatcgac
     6841 tacaaccggt tctttcggaa gttccccgcc agccccatct tcgccattaa gcacggcccc
     6901 aagtcccacc tgggccggca tctgatgaac agctttctgc acaagaacga gctggacagc
     6961 tgggccaaca gcctgggcaa ttggagcagc tcccagaacc agagagagag cggcgccaga
     7021 ctgggctaca cacacggcgg aagagatctg ccccagcccc tgtttggctt cctggccgga
     7081 tactgcgtgc ggaacgaaga gggccacatc gtgggcctgg gcctggaaaa ggacatcaac
     7141 gatctgttcg acggcatcat ggaccccctg aacgagaaag aggacaccga gatctgcgag
     7201 agctacggcg agtgggccaa gattgtgtcc aaggacgtgc tgatcttcct gaagagatac
     7261 cacagcaaga acgcctgtcg gagataccag aacagcaccc tgtatgcccg gaccttcctg
     7321 aaaaccgaga gcgtgaccct gagcggctcc aagggcagcg aggaaccttc tagccctgtg
     7381 cggatcccca tcctgagcat gggaaaggcc agcccctccg agggaagaaa gctgagagcc
     7441 agcgagcacg ccaacgacga caacgagatc gagaagatcg acagcgacag cagccagagc
     7501 gaagagatcc ctatcgagat gagcgactcc gaggacgaga caaccgccag caacatcagc
     7561 ggcatctacc tggacatgag caaggccaac tccaacgtgg tgtacagccc ccctagccag
     7621 acaggcagag ctgctggcgc cggaagaaaa agaggcgtgg gaggcagacg gaccgtggaa
     7681 agcaagcgga gaagagtgct ggcccccatc aaccggtgag cttctctagc tagagtcgat
     7741 cgacaagctc gagtttctcc ataataatgt gtgagtagtt cccagataag ggaattaggg
     7801 ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat
     7861 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa
     7921 tccagatcgc tttacgaatt cccatgggga gccaacactc gaatccccac ccgttggacc
     7981 aaacccggct cattaagcgt cggttcagat ttatttcctt tatttaaaaa aaaggaaagg
     8041 gtaaaaaata gaaaattgga aacagttaaa gcccaaaatt gtaatttacc gagaattgta
     8101 aatttacctg aaaaccctac gctatagttt cgactataaa taccaaactt aggacctcac
     8161 ttcagaatcc cctcgtcgct gcgtctctct cccgcaacct tcgattttcg tttattcgca
     8221 tccatcggag agagaaaaca atcaataagc gacctactgt atttttacaa caattaccaa
     8281 caacaacaaa caacaaacaa cattacaatt actatttaca attacaatgg ctcctaagaa
     8341 gaagaggaag gtgatgagcc agttcgacat cctgtgcaag acccccccca aggtgctggt
     8401 gcggcagttc gtggagagat tcgagaggcc cagcggcgag aagatcgcca gctgtgccgc
     8461 cgagctgacc tacctgtgct ggatgatcac ccacaacggc accgccatca agagggccac
     8521 cttcatgagc tacaacacca tcatcagcaa cagcctgagc ttcgacatcg tgaacaagag
     8581 cctgcagttc aagtacaaga cccagaaggc caccatcctg gaggccagcc tgaagaagct
     8641 gatccccgcc tgggagttca ccatcatccc ttacaacggc cagaagcacc agagcgacat
     8701 caccgacatc gtgtccagcc tgcagctgca gttcgagagc agcgaggagg ccgacaaggg
     8761 caacagccac agcaagaaga tgctgaaggc cctgctgtcc gagggcgaga gcatctggga
     8821 gatcaccgag aagatcctga acagcttcga gtacaccagc aggttcacca agaccaagac
     8881 cctgtaccag ttcctgttcc tggccacatt catcaactgc ggcaggttca gcgacatcaa
     8941 gaacgtggac cccaagagct tcaagctggt gcagaacaag tacctgggcg tgatcattca
     9001 gtgcctggtg accgagacta agacaagcgt gtccaggtaa gtttctgctt ctacctttga
     9061 tatatatata ataattatca ttaattagta gtaatataat atttcaaata tttttttcaa
     9121 aataaaagaa tgtagtatat agcaattgct tttctgtagt ttataagtgt gtatatttta
     9181 atttataact tttctaatat atgaccaaaa tttgttgatg tgcaggcaca tctacttttt
     9241 cagcgccaga ggcaggatcg accccctggt gtacctggac gagttcctga ggaacagcga
     9301 gcccgtgctg aagagagtga acaggaccgg caacagcagc agcaacaagc aggagtacca
     9361 gctgctgaag gacaacctgg tgcgcagcta caacaaggcc ctgaagaaga acgcccccta
     9421 ccccatcttc gctatcaaga acggccctaa gagccacatc ggcaggcacc tgatgaccag
     9481 ctttctgagc atgaagggcc tgaccgagct gacaaacgtg gtgggcaact ggagcgacaa
     9541 gagggcctcc gccgtggcca ggaccaccta cacccaccag atcaccgcca tccccgacca
     9601 ctacttcgcc ctggtgtcca ggtactacgc ctacgacccc atcagcaagg agatgatcgc
     9661 cctgaaggac gagactaacc ccatcgagga gtggcagcac atcgagcagc tgaagggcag
     9721 cgccgagggc agcatcagat accccgcctg gaacggcatc atcagccagg aggtgctgga
     9781 ctacctgagc agctacatca acaggcggat ctgagcttct ctagctagag tcgatcgaca
     9841 agctcgagtt tctccataat aatgtgtgag tagttcccag ataagggaat tagggttcct
     9901 atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta
     9961 aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag
    10021 atcgctcaga tgagaccgca gctggcacga caggtttccc gactggaaag cgggcagtga
    10081 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
    10141 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
    10201 ctatgaccat gattacgcca agcttgcatg cctgcaggtc gactctagag gatccccggg
    10261 taccgagctc gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
    10321 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
    10381 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
    10441 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
    10501 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
    10561 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgacggtct
    10621 cagggacacg aagtgatccg tttaaactat cagtgtttga caggatatat tggcgggtaa
    10681 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
    10741 tatccgttcg tccatttgta tgtgccagcc gcctttgcga cgctcaccgg gctggttgcc
    10801 ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg cgccagaaac gccgtcgaag
    10861 ccgtgtgcga gacaccgcgg ccgccggcgt tgtggatacc tcgcggaaaa cttggccctc
    10921 actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg cgcggcgttg
    10981 acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc ctcgcaaatc
    11041 ggcgaaaacg cctgatttta cgcgagtttc ccacagatga tgtggacaag cctggggata
    11101 agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg ggcgcgatcc
    11161 ttgacacttg aggggcagag tgctgacaga tgaggggcgc acctattgac atttgagggg
    11221 ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt cggccaccgc
    11281 taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt taaccagggc
    11341 tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc tcgaaccctc
    11401 ccggcccgct aacgcgggcc tcccatcccc ccaggggctg cgcccctcgg ccgcgaacgg
    11461 cctcacccca aaaatggcag cgctggccaa ttcccgagtg cgcggaaccc ctatttgttt
    11521 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
    11581 tcaataatat tgaaaaagga agagtatggc taaaatgaga atatcaccgg aattgaaaaa
    11641 actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg ctaaggtata
    11701 taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc ggtataaagg
    11761 gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag gaaagctgcc
    11821 tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc tcatgagtga
    11881 ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg aaaagattat
    11941 cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg attgtcccta
    12001 tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata acgatctggc
    12061 cgatgtggat tgcgaaaact gggaagagga cactccattt aaagatccgc gcgagctgta
    12121 tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg gcgacctggg
    12181 agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc ttgggagaag
    12241 cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgctca gggaggatat
    12301 cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc ctgattggga
    12361 gaaaataaaa tattatattt tactggatga attgttttag ctgtcagacc aagtttactc
    12421 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
    12481 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    12541 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    12601 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    12661 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
    12721 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    12781 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    12841 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    12901 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    12961 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    13021 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    13081 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    13141 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggcagatcc
    13201 tagatgtggc gcaacgatgc cggcgacaag caggagcgca ccgacttctt ccgcatcaag
    13261 tgttttggct ctcaggccga ggcccacggc aagtatttgg gcaaggggtc gctggtattc
    13321 gtgcagggca agattcggaa taccaagtac gagaaggacg gccagacggt ctacgggacc
    13381 gacttcattg ccgataaggt ggattatctg gacaccaagg caccaggcgg gtcaaatcag
    13441 gaataagggc acattgcccc ggcgtgagtc ggggcaatcc cgcaaggagg gtgaatgaat
    13501 cggacgtttg accggaaggc atacaggcaa gaactgatcg acgcggggtt ttccgccgag
    13561 gatgccgaaa ccatcgcaag ccgcaccgtc atgcgtgcgc cccgcgaaac cttccagtcc
    13621 gtcggctcga tggtccagca agctacggcc aagatcgagc gcgacagcgt gcaactggct
    13681 ccccctgccc tgcccgcgcc atcggccgcc gtggagcgtt cgcgtcgtct tgaacaggag
    13741 gcggcaggtt tggcgaagtc gatgaccatc gacacgcgag gaactatgac gaccaagaag
    13801 cgaaaaaccg ccggcgagga cctggcaaaa caggtcagcg aggccaagca ggccgcgttg
    13861 ctgaaacaca cgaagcagca gatcaaggaa atgcagcttt ccttgttcga tattgcgccg
    13921 tggccggaca cgatgcgagc gatgccaaac gacacggccc gctctgccct gttcaccacg
    13981 cgcaacaaga aaatcccgcg cgaggcgctg caaaacaagg tcattttcca cgtcaacaag
    14041 gacgtgaaga tcacctacac cggcgtcgag ctgcgggccg acgatgacga actggtgtgg
    14101 cagcaggtgt tggagtacgc gaagcgcacc cctatcggcg agccgatcac cttcacgttc
    14161 tacgagcttt gccaggacct gggctggtcg atcaatggcc ggtattacac gaaggccgag
    14221 gaatgcctgt cgcgcctaca ggcgacggcg atgggcttca cgtccgaccg cgttgggcac
    14281 ctggaatcgg tgtcgctgct gcaccgcttc cgcgtcctgg accgtggcaa gaaaacgtcc
    14341 cgttgccagg tcctgatcga cgaggaaatc gtcgtgctgt ttgctggcga ccactacacg
    14401 aaattcatat gggagaagta ccgcaagctg tcgccgacgg cccgacggat gttcgactat
    14461 ttcagctcgc accgggagcc gtacccgctc aagctggaaa ccttccgcct catgtgcgga
    14521 tcggattcca cccgcgtgaa gaagtggcgc gagcaggtcg gcgaagcctg cgaagagttg
    14581 cgaggcagcg gcctggtgga acacgcctgg gtcaatgatg acctggtgca ttgcaaacgc
    14641 tagggccttg tggggtcagt tccggctggg ggttcagcag cccctgctcg gatctgttgg
    14701 accggacagt agtcatggtt gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
    14761 gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga
    14821 cgcttagaca acttaataac acattgcgga cgtttttaat gtactggggt tgaacactct
//
