LOCUS       sequence-378501-       10107 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knock-in gene targeting in marmoset cells at FOXL2 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10107)
  AUTHORS   Yoshimatsu S, Kisu I, Qian E, Noce T
  TITLE     A New Horizon in Reproductive Research with Pluripotent Stem Cells: 
            Successful In Vitro Gametogenesis in Rodents, Its Application to 
            Large Animals, and Future In Vitro Reconstitution of Reproductive 
            Organs Such as ""Uteroid"" and ""Oviductoid"".
  JOURNAL   Biology (Basel). 2022 Jun 29;11(7):987. doi: 
            10.3390/biology11070987.
  PUBMED    36101367
REFERENCE   2  (bases 1 to 10107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biology 
            (Basel)."; date: "2022-06-29"; volume: "11(7):987. doi"; pages: " 
            10.3390/biology11070987"
COMMENT     Sequence Label: pKI-cjFOXL2-coemiRFP670-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..10107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(941..1732,1733..1801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(1819..2613)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1930..1949)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2540..2559
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      complement(2957..3385)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3067..3088)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3279..3298
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3511..3533
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3525..3542
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3526..3542
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3549..3568
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3549..3567
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4176..4232
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5169..5210
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    5255..5288
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    5384..5439
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     promoter        5810..6309
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5845..5868)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6242..6261
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6349..6948
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6349..6368)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6845..6865
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    7505..7538
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             8549..9121
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          9198..9263
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    9476..9531
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9887..9903)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9887..9903)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9900..9922)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9911..9927
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9935..9941,9942..9959,9960..9965))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9980..10001
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaattc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
     1861 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
     1921 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
     1981 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
     2041 ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc
     2101 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
     2161 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
     2221 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     2281 ctcggcagga gcaaggtggg atgacaggag atcctgcccc ggcacttcgc ccaatagcag
     2341 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
     2401 ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc
     2461 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
     2521 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
     2581 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg
     2641 atcagatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac
     2701 tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc
     2761 tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt
     2821 tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg
     2881 tcagcaccgt ttctgcggac tggctttcta cgtgttccgc ttcctttagc agcccttgcg
     2941 ccctgaattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     3001 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     3061 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     3121 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     3181 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     3241 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     3301 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     3361 cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag
     3421 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     3481 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     3541 gtgaattgta atacgactca ctatagggcg aattgggccc tctagatgca tgctcgagcg
     3601 gctacctggc gccccccaag tacctgcaat ccggtttcct caacaactcg tggccgctgc
     3661 cgcagcctcc ctcacccatg ccctatgcct cctgccagat ggcggcggcc gcggcggctg
     3721 cggcggctgc ggctgcggcc gcgggcccgg gtagccctgg cgcggccgct gtggtcaagg
     3781 ggctggcggg cccggccgcc tcgtacgggc cgtacacacg cgtgcagagc atggcgctgc
     3841 cccccggcgt agtgaactcg tacaacggct tgggaggccc gccggccgcg cctccgcccc
     3901 cgccgcaccc ccacccgcat ccgcacgcac accatctgca cgcggccgcc gcaccgccgc
     3961 cggccccacc gcaccacggg gctgccgcac cgcccccggg ccagctcagc cccgccagcc
     4021 cagccaccgc cgcgcccccg gcgccggcgc ccagcagtgc gcccggcctg cagttcgctt
     4081 gcgcccggca gcccgagctc gccatgatgc actgctctta ctgggaccac gacagcaaga
     4141 cgggcgccct gcattcgcgc ctagatctcg ctagcgccac taacttctcc ctgttgaaac
     4201 aagcagggga tgtcgaagag aatcccgggc caatggctga aggatctgtc gcccgccaac
     4261 cagatttgct cacctgcgag cacgaggaga tacatctggc gggatccatt cagccacacg
     4321 gtgcgttgct cgttgttagt gagcatgacc acagagtaat acaagccagt gcgaacgctg
     4381 ctgagttctt gaatcttggt tcagtcctgg gcgtccctct cgcggaaatc gacggtgacc
     4441 tcctgattaa aatacttccg cacctggacc ctacagcgga gggtatgcca gtggctgttc
     4501 gatgccgaat aggaaaccca tcaacggaat attgtggcct tatgcaccga cctccagagg
     4561 gtgggttgat aatagaactc gagagggccg gcccctcaat cgatctgagc gggactcttg
     4621 cccctgctct cgagcgcatc agaactgcag gttctctcag ggcgctctgt gatgacactg
     4681 ttttgctctt tcaacaatgt accggctatg acagagttat ggtatataga ttcgatgaac
     4741 agggacacgg gctcgttttt tctgagtgcc atgtgccagg tctcgaaagc tatttcggga
     4801 atagatatcc ctcttcaacg gttcctcaga tggctcggca gctctacgtg cgccagaggg
     4861 tgcgggtact tgtggatgtt acttatcagc ctgtcccgct tgagcctcgc ctcagcccac
     4921 ttacgggcag agacctggat atgtcaggct gcttcctcag gtctatgtcc ccgtgccatc
     4981 tgcagttcct gaaagatatg ggggtaagag ctacgttggc agtctccctc gtagtaggtg
     5041 gaaaactgtg gggtcttgta gtttgtcatc actatctgcc acgctttata cgattcgagt
     5101 tgagggcaat ttgtaagcga ttggcggagc gaattgccac caggatcacg gcgctcgaaa
     5161 gcggatccgg taagcctatc cctaaccctc tcctcggtct cgattctacg taatgtacaa
     5221 gtaagaattc cgatcatatt caataaccct taatataact tcgtataatg tatgctatac
     5281 gaagttatta ggtctgaaga ggagtttacg tccagccatc tttttccctc tgccaaaaat
     5341 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
     5401 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     5461 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
     5521 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc
     5581 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata
     5641 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta
     5701 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg
     5761 agatccctcg acctgcagaa gcttaggatc tcgacctcga aattctaccg ggtaggggag
     5821 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     5881 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     5941 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     6001 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     6061 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     6121 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
     6181 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
     6241 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     6301 ggcctttcga cctgcatcca tctagatctc gagcagctga agcttaccat gaccgagtac
     6361 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     6421 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     6481 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     6541 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     6601 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     6661 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     6721 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     6781 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     6841 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     6901 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac
     6961 gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcgatgat atcagatccc
     7021 cgggatgcag aaattgatga tctattaaac aataaagatg tccactaaaa tggaagtttt
     7081 tcctgtcata ctttgttaag aagggtgaga acagagtacc tacattttga atggaaggat
     7141 tggagctacg ggggtggggg tggggtggga ttagataaat gcctgctctt tactgaaggc
     7201 tctttactat tgctttatga taatgtttca tagttggata tcataattta aacaagcaaa
     7261 accaaattaa gggccagctc attcctccca ctcatgatct atagatctat agatctctcg
     7321 tgggatcatt gtttttctct tgattcccac tttgtggttc taagtactgt ggtttccaaa
     7381 tgtgtcagtt tcatagcctg aagaacgaga tcagcagcct ctgttccaca tacacttcat
     7441 tctcagtatt gttttgccaa gttctaattc catcagaagc tggtcgagat ccggaaccct
     7501 taatataact tcgtataatg tatgctatac gaagttatta ggtccctcga agaggttcac
     7561 taggcgcgga gcccaccgca tgacagttcg gacggatgcg aggatgcagg aacgcgcggc
     7621 gccggccctg gtcgcagccg cagccgccag ccgaacctcg caccctctgg ggccgcctct
     7681 ggagccagtg cccagggtcc ctctgtgctt tttcgctctc ctcagctcct ctcgcccctc
     7741 tttgtccctc tgcttgtcct gtgctcacct ccgtgacctt tatgcccttg cactcccccg
     7801 gcctgaagcc gcctctctgc gcgctttctg ctgggctctt ctctttccgg agccccagcg
     7861 tctcctgccc aaattgaccg cggaaagcgc ccaggaccga ggtgcgaccc ggcgtcggcg
     7921 gcgcagacct cttggccttc tctcacaggt cggtgcgcta gcgctcggcg ttccccgccc
     7981 gactgccgtg cagtctttgg ctacacgcgc cggactggat tggcggcggg acccgcgccg
     8041 cgggagaaag ggacggacca atttgtgtgt ttgctccgcg aaccctcttg aaggtgttca
     8101 gaagctgctt gccgcggggc ccactaggcg ttgcgggggt taggaaccag cgggagccag
     8161 ggcagcccgg tccgagctgt attgtttcct gttcccaggg gtcccaacac tgggctgcag
     8221 atacgcaatc actgctgcct ccccaggagc tcctggtcga gcagtgtggt tttcaagagg
     8281 aagcaaaaag cctctccacc caggcctgga atgtttccac ccaatgtcga gcagtgtggt
     8341 tttgcaagag gaagcaaaaa gcctctccac ccaggcctgg aatgtttcca cccaatgtcg
     8401 agcaaacccc gcccagcgtc ttgtcattgg cgaattcgaa cacgcagatg cagtcggggc
     8461 ggcgcggtcc caggtccact tcgcatatta aggtgacgcg tgtggcctcg aacaccgagc
     8521 gaccctgcag gtcctcgcca tggatcctga tgatgttgtt gattcttcta aatcttttgt
     8581 gatggaaaac ttttcttcgt accacgggac taaacctggt tatgtagatt ccattcaaaa
     8641 aggtatacaa aagccaaaat ctggtacaca aggaaattat gacgatgatt ggaaagggtt
     8701 ttatagtacc gacaataaat acgacgctgc gggatactct gtagataatg aaaacccgct
     8761 ctctggaaaa gctggaggcg tggtcaaagt gacgtatcca ggactgacga aggttctcgc
     8821 actaaaagtg gataatgccg aaactattaa gaaagagtta ggtttaagtc tcactgaacc
     8881 gttgatggag caagtcggaa cggaagagtt tatcaaaagg ttcggtgatg gtgcttcgcg
     8941 tgtagtgctc agccttccct tcgctgaggg gagttctagc gttgaatata ttaataactg
     9001 ggaacaggcg aaagcgttaa gcgtagaact tgagattaat tttgaaaccc gtggaaaacg
     9061 tggccaagat gcgatgtatg agtatatggc tcaagcctgt gcaggaaatc gtgtcaggcg
     9121 atctctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     9181 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     9241 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     9301 ggaatgcaga tccactagag tcgaagcggc ttcactcctc aggtgcaggc tgcctatcag
     9361 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     9421 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     9481 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     9541 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     9601 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     9661 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     9721 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     9781 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     9841 cttctcttat ggagatcagc tcggtaccaa gcttggcgta atcatggtca tagctgtttc
     9901 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     9961 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
    10021 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    10081 ggagaggcgg tttgcgtatt gggcgct
//
