LOCUS       sequence-378815-        3983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTR plasmid Triticum aestivum SRO1, full CDS, cultivar JN177, no 
            stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Vogt S, Feijs K, Hosch S, De Masi R, Lintermann R, Loll B, 
            Wirthmueller L
  TITLE     The superior salinity tolerance of bread wheat cultivar Shanrong No.
            3 is unlikely to be caused by elevated Ta-sro1 poly-(ADP-ribose) 
            polymerase activity.
  JOURNAL   Plant Cell. 2022 Aug 18. pii: 6671231. doi: 10.1093/plcell/koac261.
  PUBMED    35980152
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2022 Aug 18. pii: 6671231. doi: 10.1093/plcell/koac261."
COMMENT     Sequence Label: pENTR4 Ta-SRO1 JN177
FEATURES             Location/Qualifiers
     source          1..3983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      119..205
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      297..324
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     332..355
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    374..473
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2228..2327)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2439..2458)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2450..3259
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2517..2536)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3349..3937
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3838..3857
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
       61 ctagcatgga tctcggggac gtctaactac taagcgagag tagggaactg ccaggcatca
      121 aataaaacga aaggctcagt cggaagactg ggcctttcgt tttatctgtt gtttgtcggt
      181 gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg tgaagcaacg
      241 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ctaagcagaa
      301 ggccatcctg acggatggcc tttttgcgtt tctacaaact cttcctgtta gttagttact
      361 taagctcggg ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
      421 aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagcag gctccacatg
      481 gaaaggaaga ctggaatggt actggatgaa catgtcctga aagctactga tcgcaagagg
      541 aaacatgagt cgtctgtgaa gagtactggc acagatgatt ttgctgaggt ctctcagcat
      601 gctgatggtt ctgctgtcct taagaagtct aaaatgacct cctctgctgg tgatattctg
      661 gagtgctata agaacttcaa gacaagcggt tcgcccgtgc gcgtgctttg ttaccaccag
      721 ggtaaatgga gggactttcc agagcatgtt gtgaatctgg tacagcagga cttccagttg
      781 aagaggccaa ttactaatgc tgtattgaag aaccagcaag tcctgttgga ctttatgcac
      841 atggtctgca ttgattcttc gatgaccaca aacaagccaa tagcatggat tgatgttcat
      901 ggtaaccgct tctttccaga gttatgtgct ggactggtaa catctaaacc atctcagcat
      961 gggaaaagtg accccagtgg taaatctaaa gctggtgagt gtgctgggac tttgattttg
     1021 acagctgaag ctgaaagctc gagttcagat tctgttgatg cggtactccc tcatgctaag
     1081 aaggttaata acatactgga ggaaggacag gaggtacata attatgctgc tgttgaaaat
     1141 aaagctggtc ctgcaatctg tttgaatgaa cctgctagtg gaaacataca tgctgctact
     1201 tataagcaga aaattggcca acctggtgat tcagctgtgc gcaagttatt gcttgaaggg
     1261 gcaggccaac cttttagtga agaaaatatt attggcatct acagaactcc actggtagat
     1321 cagcaagggc gggctcgctt caatcttttc cagaaggaac ttgaggccac caaaatgcat
     1381 cgtggcaacg caaatgtgcg ctacgcgtgg ctaccttgtt ccaaggatac tatggaggag
     1441 atgatgatgc gtggtgttct ggaagttaca aaacccatgc tggggccagt gtatggcatt
     1501 ggtgcacatc ttgctccagc gaactgtgcc caaacctgcg ctagctactc agatattgac
     1561 gaaaatggca tcatgaggat gatgctgtgc cgtgttataa tgggtaatgt tgaggttgta
     1621 cttcctggat caaagcaatt ccagccaacc aatgaaagat ttgatagtgg tgtggatgat
     1681 cttcaaaagc caaagcacta tatcatatgg gatgctaatg tgcatcgaca catctatgct
     1741 gaatatgctg ttgttatcaa agcaccttcc atgaccaatg aatacttggc tagagaagat
     1801 actgcatcca acatatctga gataagaaat tctggttcag cagaaagtgt aatcaaggac
     1861 gatagttccg aaaccatggc atctccagct gataaacaac aagcacccag gtttgggcgt
     1921 gccccaactc gaaggcctcc tacttcaccc tggatgcccc tcccaatgct tttcgctgcc
     1981 atctccacaa aagttcctcg ttctgacatg gatgtaatcc atggacacta tgaagaattc
     2041 aagaggagaa agataagcag gcccgagttt gtgagacggc taagacagat cttcggcgac
     2101 aagctgctgg tttctacagt aatgaggctg caacctaagg tagtggcacc catggcaggt
     2161 gctgaagtgc taccaagagg agcacccggc acgggaggga gcagcacctc caactcgaga
     2221 tatctagacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     2281 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgca
     2341 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     2401 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     2461 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     2521 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
     2581 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     2641 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     2701 ttttatccgt actcctggtg atgcatggtt actcaccact gcgatccccg gaaaaacagc
     2761 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     2821 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     2881 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     2941 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     3001 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     3061 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     3121 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     3181 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     3241 gctcgatgag tttttctaat cagaattggt taattggttg taacattatt cagattgggc
     3301 cccgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3361 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3421 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3481 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3541 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3601 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3661 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3721 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     3781 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3841 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3901 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3961 acggttcctg gccttttgct ggc
//
