LOCUS       sequence-378846-        4016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector expressing sgGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Duarte F, Ramosaj M, Hasanovic E, Regio S, Sipion M, Rey M, Deglon N
  TITLE     Semi-automated workflows to quantify AAV transduction in various 
            brain areas and predict gene editing outcome for neurological 
            disorders.
  JOURNAL   Mol Ther Methods Clin Dev. 2023 Mar 27;29:254-270. doi: 
            10.1016/j.omtm.2023.03.013. eCollection 2023 Jun 8.
  PUBMED    37090478
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2023-03-27"; volume: "29:254-270. doi"; 
            pages: " 10.1016/j.omtm.2023.03.013. eCollection 2023 Jun 8"
COMMENT     Sequence Label: pAAV2ss-U6-sgGFP
FEATURES             Location/Qualifiers
     source          1..4016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     193..209
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     194..210
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    233..257
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        298..546
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     298..318
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     469..488
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    complement(713..737)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(797..814)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    803..1010
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1429..2017)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1509..1528)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2188..2979,2980..3048))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2811..2830
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3049..3153)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3221..3239
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3277..3299)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3399..3418
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(3485..3940)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3622..3643)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3834..3853
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gggggggggg ggssrmcwmc wycacywsks kbksykchsm ysmgmkckcw caytgrggcc
       61 gsccgggcaa agcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga
      121 gcgcgcagag agggagtggc caactccatc actaggggtt cctcagatct gaattcggta
      181 ccgggccccc cctcgaggtc gacggtatcg ataagcttga tatcgaatta tcacaagttt
      241 gtacaaaaaa gcaggcttca tcgatcaatg cggccgctgg gatccaagct tccatgggag
      301 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
      361 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
      421 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
      481 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
      541 aacaccgggc gaggagctgt tcaccggttt cagagctatg ctggaaacag catagcaagt
      601 tgaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttc
      661 atagccatgg gcggccgctc tagatggaag cttctcgaga ctagtaggcc tgacccagct
      721 ttcttgtaca aagtggtgat aattcctgca gcccggggga tccactagtt ctagtcgact
      781 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
      841 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
      901 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
      961 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga gagatctgag
     1021 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgactcg
     1081 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagcty askyrwmatt ctattctggg
     1141 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     1201 ggagagatct gaggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
     1261 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     1321 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     1381 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     1441 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1501 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1561 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1621 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1681 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1741 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1801 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1861 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1921 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1981 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     2041 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     2101 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     2161 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     2221 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     2281 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     2341 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     2401 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     2461 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     2521 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     2581 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     2641 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     2701 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     2761 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     2821 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     2881 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     2941 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     3001 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     3061 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     3121 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     3181 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     3241 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     3301 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     3361 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     3421 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     3481 caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     3541 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     3601 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     3661 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     3721 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     3781 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     3841 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     3901 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc
     3961 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag gctgca
//
