LOCUS       sequence-379630-       11185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nimble cloning-compatible vector for plant BiFC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11185)
  AUTHORS   Yan P, Tuo D, Shen W, Deng H, Zhou P, Gao X
  TITLE     A Nimble Cloning-compatible vector system for high-throughput gene 
            functional analysis in plants.
  JOURNAL   Plant Commun. 2023 Mar 13;4(2):100471. doi: 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9.
  PUBMED    36352791
REFERENCE   2  (bases 1 to 11185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun."; date: "2023-03-13"; volume: "4(2):100471. doi"; pages: " 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9"
COMMENT     Sequence Label: pNC-BiFC-Vnc
FEATURES             Location/Qualifiers
     source          1..11185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     promoter        complement(1238..1618)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2614..2959
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2902..2922
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    2989..3043
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        3225..3327
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             3358..3663
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3542..3561
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             join(3736..3738,3739..3741,3742..4254)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(3781..3802)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4042..4061)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     terminator      4384..4636
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4687..4704)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4687..4703)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4696..4718)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4906..4930
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6231..6860
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7289..8362
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8428..8622
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8858..8880)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8966..9106
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9021..9040
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9121..9138)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9292..9880)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9372..9391)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9967..10761)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta cctcacttgt
      421 acagctcgtc catgccgaga gtaattccag cagctgtaac aaattccaaa agaaccatgt
      481 gatctctctt ttcattaggg tctttagaca acttagattg agtagacaaa tagtggttat
      541 caggcaaaag aactggtcca tcaccaatag gagtattttg ctgataatga tcagccaatt
      601 gaactccacc atcttcaata ttatgtctaa tcttaaaatt agctttaatt ccattctttt
      661 gtttatcagc agtaatataa acattatctg aaaagtaatt atactccaac ttatgaccca
      721 aaatatttcc atcttcttta aagtcaatac ccttcaactc aattctatta accaaagtat
      781 ctccttcaaa cttcacttca gctctagtct tataatttcc atcgtcctta aagaagatag
      841 ttctctcttg aacatatcct tcaggcatag cagacttaaa gaaatcatgt tgtttcatgt
      901 ggtcagggta tctagcaaaa cattgaactc cccaagacaa agttgtcaca agagtcggcc
      961 aaggaaccgg caactttcca gttgtacaaa taaacttcaa agtgagcttt ccataagtag
     1021 catctccctc accctcacca ctaacagaaa acttatgtcc attaacgtcg ccatccaatt
     1081 caacaagaat aggcacaact ccagtaaaca gctcctcgcc cttgctcact gagaaagatg
     1141 aactgtggaa cacgaagtag atgataagga acaaactgtt gagaagcaac atatacagga
     1201 atatcttcag aatcttaggg ttccgcttac tcattcgcga tttggtgtat cgagattggt
     1261 tatgaaattc agatgctagt gtaatgtatt ggtaatttgg gaagatataa taggaagcaa
     1321 ggctatttat ccatttctga aaaggcgaaa tggcgtcacc gcgagcgtca cgcgcattcc
     1381 gttcttgctg taaagcgttg tttggtacac ttttgactag cgaggcttgg cgtgtcagcg
     1441 tatctattca aaagtcgtta atggctgcgg atcaagaaaa agttggaata gaaacagaat
     1501 acccgcgaaa ttcaggcccg gttgccatgt cctacacgcc gaaataaacg accaaattag
     1561 tagaaaaata aaaactgact cggatactta cgtcacgtct tgcgcactga tttgaaaaat
     1621 ctcagaattc caatcccaca aaaatctgag cttaacagca cagttgctcc tctcagagca
     1681 gaatcgggta ttcaacaccc tcatatcaac tactacgttg tgtataacgg tccacatgcc
     1741 ggtatatacg atgactgggg ttgtacaaag gcggcaacaa acggcgttcc cggagttgca
     1801 cacaagaaat ttgccactat tacagaggca agagcagcag ctgacgcgta cacaacaagt
     1861 cagcaaacag acaggttgaa cttcatcccc aaaggagaag ctcaactcaa gcccaagagc
     1921 tttgctaagg ccctaacaag cccaccaaag caaaaagccc actggctcac gctaggaacc
     1981 aaaaggccca gcagtgatcc agccccaaaa gagatctcct ttgccccgga gattacaatg
     2041 gacgatttcc tctatcttta cgatctagga aggaagttcg aaggtgaagg tgacgacact
     2101 atgttcacca ctgataatga gaaggttagc ctcttcaatt tcagaaagaa tgctgaccca
     2161 cagatggtta gagaggccta cgcagcaggt ctcatcaaga cgatctaccc gagtaacaat
     2221 ctccaggaga tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact
     2281 aattgcatca agaacacaga gaaagacata tttctcaaga tcagaagtac tattccagta
     2341 tggacgattc aaggcttgct tcataaacca aggcaagtaa tagagattgg agtctctaaa
     2401 aaggtagttc ctactgaatc taaggccatg catggagtct aagattcaaa tcgaggatct
     2461 aacagaactc gccgtgaaga ctggcgaaca gttcatacag agtcttttac gactcaatga
     2521 caagaagaaa atcttcgtca acatggtgga gcacgacact ctggtctact ccaaaaatgt
     2581 caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa ggataatttc
     2641 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga
     2701 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta tcattcaaga
     2761 tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
     2821 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgacatct ccactgacgt
     2881 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
     2941 atttcatttg gagaggacac gctcgagtat aagagctcta tttttacaac aattaccaac
     3001 aacaacaaac aacaaacaac attacaatta catttacaat taccagtggt ctctgtccag
     3061 tcctggcctc gtcggccatt ctcgactaag ttggcagcat cacccgacgc actttgcgcc
     3121 gaataaatac ctgtgacgga agatcacttc gcagaataaa taaatcctgg tgtccctgtt
     3181 gataccggga agccctgggc caacttttgg cgaaaatgag acgttgatcg gcacgtaaga
     3241 ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg
     3301 agattttcag gagctaagga agctaaactt ttgctgacga gaacagggac tggtgaaatg
     3361 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
     3421 agtgatatta ttgacacgcc tgggcgacgg atggtgatcc ccctggccag tgcacgtctg
     3481 ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
     3541 cgcatgatga ccaccgatat ggccagtgtg ccggtatccg ttatcgggga agaagtggct
     3601 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
     3661 taaatgtcag gctcccttat acacagggcc agtctggcca cttgtggtct gctgagaccc
     3721 ggaggtgggt caggaatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     3781 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     3841 ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc
     3901 gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac
     3961 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     4021 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     4081 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     4141 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc
     4201 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc
     4261 ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     4321 ctgcccgaca accactacct gagctaccag tccaagctga gcaaataatc tagacccaaa
     4381 taggatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     4441 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     4501 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     4561 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     4621 tctatgttac tagatcgtta acgatatgtc gaggctcagc aggacctgca ggcatgcaag
     4681 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     4741 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     4801 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgctagagca gcttgagctt
     4861 ggatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca ggatatattg
     4921 gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg
     4981 aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga
     5041 tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt
     5101 gcagccgtct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc
     5161 cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg
     5221 cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta
     5281 tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc
     5341 cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct
     5401 ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg
     5461 cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc
     5521 gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt
     5581 gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg
     5641 gagcgggcgc gaggccgcca aggcgcgagg cgtgaagttt ggcccccgcc ctaccctcac
     5701 cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga
     5761 ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga
     5821 ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga
     5881 ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac
     5941 caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg
     6001 gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg
     6061 gccggtttgt ctgatgccaa gctggcggcc tggccggcga gcttggccgc tgaagaaacc
     6121 gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc
     6181 gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta
     6241 tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc
     6301 gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg
     6361 cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc
     6421 cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag
     6481 cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc
     6541 cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc
     6601 agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca
     6661 aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc
     6721 ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg
     6781 ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa
     6841 ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac
     6901 gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc
     6961 agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct
     7021 gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc
     7081 gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag
     7141 gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg
     7201 gaggaacggg cggttggcca ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca
     7261 ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca
     7321 aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggcggcgc aggccgccca
     7381 gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg
     7441 aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc
     7501 caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga
     7561 tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg
     7621 cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggcggg
     7681 catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc
     7741 catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt
     7801 tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt
     7861 agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa
     7921 gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt
     7981 aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg
     8041 cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat
     8101 cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga
     8161 agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa
     8221 gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa
     8281 ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg
     8341 cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg
     8401 ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc
     8461 gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc
     8521 acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt
     8581 aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac
     8641 agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc
     8701 ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa
     8761 tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg
     8821 caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     8881 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     8941 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
     9001 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     9061 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     9121 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     9181 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     9241 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     9301 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     9361 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     9421 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     9481 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     9541 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9601 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9661 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9721 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9781 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9841 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9901 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc
     9961 taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt
    10021 gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat
    10081 cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc
    10141 aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg
    10201 ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg
    10261 atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt
    10321 cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga
    10381 tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg
    10441 gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag
    10501 attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc
    10561 cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct
    10621 gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg
    10681 agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg
    10741 tgatattctc attttagcca tttattattt ccttcctctt ttctacagta tttaaagata
    10801 ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac
    10861 cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta
    10921 tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg tcatcgttac
    10981 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
    11041 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
    11101 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
    11161 ggtcggggag ctgttggctg gctgg
//
