LOCUS       sequence-379825-       10919 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 MoClo plasmid containing an aTC inducible dCas9 for CRISPRi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10919)
  AUTHORS   Brychcy M, Kokodynski A, Lloyd D, Godoy VG
  TITLE     AspFlex: Molecular Tools to Study Gene Expression and Regulation in 
            Acinetobacter baumannii.
  JOURNAL   ACS Synth Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167.
  PUBMED    37587063
REFERENCE   2  (bases 1 to 10919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167."
COMMENT     Sequence Label: pMB1-A-ptet-dCas9
FEATURES             Location/Qualifiers
     source          1..10919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(16..642)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     547..566
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        658..713
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    694..712
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(762..788,789..791,792..3278,3279..3281,3282..4868)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEENPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      4881..4908
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(8867..8884)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9038..9626)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9118..9137)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9797..10588,10589..10657))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10420..10439
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10658..10762)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10830..10848
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(10852..10895)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    10898..10917
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 cgtctcaatc tctatttaag acccactttc acatttaagt tgtttttcta atccgcatat
       61 gatcaattca aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat
      121 agcttgtcgt aataatggcg gcatactatc agtagtaggt gtttcccttt cttctttagc
      181 gacttgatgc tcttgatctt ccaatacgca acctaaagta aaatgcccca cagcgctgag
      241 tgcatataat gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta attgattttc
      301 gagagtttca tactgttttt ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg
      361 atgacttagt aaagcacatc taaaactttt agcgttatta cgtaaaaaat cttgccagct
      421 ttccccttct aaagggcaaa agtgagtatg gtgcctatct aacatctcaa tggctaaggc
      481 gtcgagcaaa gcccgcttat tttttacatg ccaatacaat gtaggctgct ctacacctag
      541 cttctgggcg agtttacggg ttgttaaacc ttcgattccg acctcattaa gcagctctaa
      601 tgcgctgtta atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt
      661 gacactctat cgttgataga gttattttac cactccctat cagtgataga gaaaagaatt
      721 caaaagatct gtactttaac tttaagaagg agatatacat aatggataag aaatactcaa
      781 taggcttagc tatcggcaca aatagcgtcg gatgggcggt gatcactgat gaatataagg
      841 ttccgtctaa aaagttcaag gttctgggaa atacagaccg ccacagtatc aaaaaaaatc
      901 ttataggggc tcttttattt gacagtggag agacagcgga agcgactcgt cttaaacgga
      961 cagctcgtag aaggtataca cgtcggaaga atcgtatttg ttatctacag gagatttttt
     1021 caaatgagat ggcgaaagta gatgatagtt tctttcatcg acttgaagag tcttttttgg
     1081 tggaagaaga caagaagcat gaacgtcatc ctatttttgg aaatatagta gatgaagttg
     1141 cttatcatga gaaatatcca actatctatc atctgcgaaa aaaattggta gattctactg
     1201 ataaagcgga tttgcgctta atctatttgg ccttagcgca tatgattaag tttcgtggtc
     1261 attttttgat tgagggagat ttaaatcctg ataatagtga tgtggacaaa ctatttatcc
     1321 agttggtaca aacctacaat caattatttg aagaaaaccc tattaacgca agtggagtag
     1381 atgctaaagc gattctttct gcacgattga gtaaatcaag acgattagaa aatctcattg
     1441 ctcagctccc cggtgagaag aaaaatggct tatttgggaa tctcattgct ttgtcattgg
     1501 gtttgacccc taattttaaa tcaaattttg atttggcaga agatgctaaa ttacagcttt
     1561 caaaagatac ttacgatgat gatttagata atttattggc gcaaattgga gatcaatatg
     1621 ctgatttgtt tttggcagct aagaatttat cagatgctat tttactttca gatatcctaa
     1681 gagtaaatac tgaaataact aaggctcccc tatcagcttc aatgattaaa cgctacgatg
     1741 aacatcatca agacttgact cttttaaaag ctttagttcg acaacaactt ccagaaaagt
     1801 ataaagaaat cttttttgat caatcaaaaa acggatatgc aggttatatt gatgggggag
     1861 ctagccaaga agaattttat aaatttatca aaccaatttt agaaaaaatg gatggtactg
     1921 aggaattatt ggtgaaacta aatcgtgaag atttgctgcg caagcaacgg acctttgaca
     1981 acggctctat tccccatcaa attcacttgg gtgagctgca tgctattttg agaagacaag
     2041 aagactttta tccattttta aaagacaatc gtgagaagat tgaaaaaatc ttgacttttc
     2101 gaattcctta ttatgttggt ccattggcgc gtggcaatag tcgttttgca tggatgactc
     2161 ggaagtctga agaaacaatt accccatgga attttgaaga agttgtcgat aaaggtgctt
     2221 cagctcaatc atttattgaa cgcatgacaa actttgataa aaatcttcca aatgaaaaag
     2281 tactaccaaa acatagtttg ctttatgagt attttacggt ttataacgaa ttgacaaagg
     2341 tcaaatatgt tactgaagga atgcgaaaac cagcatttct ttcaggtgaa cagaagaaag
     2401 ccattgttga tttactcttc aaaacaaatc gaaaagtaac cgttaagcaa ttaaaagaag
     2461 attatttcaa aaaaatagaa tgttttgata gtgttgaaat ttcaggagtt gaagatagat
     2521 ttaatgcttc attaggtacc taccatgatt tgctaaaaat tattaaagat aaagattttt
     2581 tggataatga agaaaatgaa gatatcttag aggatattgt tttaacattg accttatttg
     2641 aagataggga gatgattgag gaaagactta aaacatatgc tcacctcttt gatgataagg
     2701 tgatgaaaca gcttaaacgt cgccgttata ctggttgggg acgtttgtct cgaaaattga
     2761 ttaatggtat tagggataag caatctggca aaacaatatt agattttttg aaatcagatg
     2821 gttttgccaa tcgcaatttt atgcagctga tccatgatga tagtttgaca tttaaagaag
     2881 acattcaaaa agcacaagtg tctggacaag gcgatagttt acatgaacat attgcaaatt
     2941 tagctggtag ccctgctatt aaaaaaggta ttttacagac tgtaaaagtt gttgatgaat
     3001 tggtcaaagt aatggggcgg cataagccag aaaatatcgt tattgaaatg gcacgtgaaa
     3061 atcagacaac tcaaaagggc cagaaaaatt cgcgagagcg tatgaaacga atcgaagaag
     3121 gtatcaaaga attaggaagt cagattctta aagagcatcc tgttgaaaat actcaattgc
     3181 aaaatgaaaa gctctatctc tattatctcc aaaatggaag agacatgtat gtggaccaag
     3241 aattagatat taatcgttta agtgattatg atgtcgatgc cattgttcca caaagtttcc
     3301 ttaaagacga ttcaatagac aataaggtct taacgcgttc tgataaaaat cgtggtaaat
     3361 cggataacgt tccaagtgaa gaagtagtca aaaagatgaa aaactattgg agacaacttc
     3421 taaacgccaa gttaatcact caacgtaagt ttgataattt aacgaaagct gaacgtggag
     3481 gtttgagtga acttgataaa gctggtttta tcaaacgcca attggttgaa actcgccaaa
     3541 tcactaagca tgtggcacaa attttggata gtcgcatgaa tactaaatac gatgaaaatg
     3601 ataaacttat tcgagaggtt aaagtgatta ccttaaaatc taaattagtt tctgacttcc
     3661 gaaaagattt ccaattctat aaagtacgtg agattaacaa ttaccatcat gcccatgatg
     3721 cgtatctaaa tgccgtcgtt ggaactgctt tgattaagaa atatccaaaa cttgaatcgg
     3781 agtttgtcta tggtgattat aaagtttatg atgttcgtaa aatgattgct aagtctgagc
     3841 aagaaatagg caaagcaacc gcaaaatatt tcttttactc taatatcatg aacttcttca
     3901 aaacagaaat tacacttgca aatggagaga ttcgcaaacg ccctctaatc gaaactaatg
     3961 gggaaactgg agaaattgtc tgggataaag ggcgagattt tgccacagtg cgcaaagtat
     4021 tgtccatgcc ccaagtcaat attgtcaaga aaacagaagt acagacaggc ggattctcca
     4081 aggagtcaat tttaccaaaa agaaattcgg acaagcttat tgctcgtaaa aaagactggg
     4141 atccaaaaaa atatggtggt tttgatagtc caacggtagc ttattcagtc ctagtggttg
     4201 ctaaggtgga aaaagggaaa tcgaagaagt taaaatccgt taaagagtta ctagggatca
     4261 caattatgga aagaagttcc tttgaagaaa atccgattga ctttttagaa gctaaaggat
     4321 ataaggaagt taaaaaagac ttaatcatta aactacctaa atatagtctt tttgagttag
     4381 aaaacggtcg taaacggatg ctggctagtg ccggagaatt acaaaaagga aatgagctgg
     4441 ctctgccaag caaatatgtg aattttttat atttagctag tcattatgaa aagttgaagg
     4501 gtagtccaga agataacgaa caaaaacaat tgtttgtgga gcagcataag cattatttag
     4561 atgagattat tgagcaaatc agtgaatttt ctaagcgtgt tattttagca gatgccaatt
     4621 tagataaagt tcttagtgca tataacaaac atagagacaa accaatacgt gaacaagcag
     4681 aaaatattat tcatttattt acgttgacga atcttggagc tcccgctgct tttaaatatt
     4741 ttgatacaac aattgatcgt aaacgatata cgtctacaaa agaagtttta gatgccactc
     4801 ttatccatca atccatcact ggtctttatg aaacacgcat tgatttgagt cagctaggag
     4861 gtgactaact cgatcacact ggctcacctt cgggtgggcc tttctgcgtt tatatgtttg
     4921 ccagagacgc tgcagttaac aagttgcctg acgctgtgtc ttatccagtt catgcggcaa
     4981 tgctatctct acttcctttg ctaatcttgc ctcctgtttg atgtcaccgt tcttttttag
     5041 ttcggattgc tctacccgat tccatagggt ttgacgatct aacatgtcag gacttgcccc
     5101 agttggggca aaaatttggg tgtattcaat gcctgttttt ttggtgtagt cctgctcttt
     5161 tccgtacgta tcacagtaca atttttcgcc tgctcggtat gctgcacatg ccacgattga
     5221 gcgaccatct gacctcgaaa tgttctgcat ttcacaatgg taaattgcca ttttcatcac
     5281 ctgtttatta acagccgccc caagttttgg gcggttgggg tgtcggggtt tccctgaccg
     5341 tacttgcaaa atttggcgta gcaaaaattt tgcgtaagtg cgcccttcgg gaactctgcg
     5401 aggctaaaag caaaagcaag agcaataagg ctttgacttt gttttttgat tttgctcgct
     5461 gcgctcggat cttgatgtaa gtcgagtttt tgaagtagaa cacttttcac attgatgatg
     5521 gttcatactt cggaaaatag gttgagacac aggcattaaa aatggtcaaa aaaacagcta
     5581 tggaattggg tcaaatgctc gatgaagaaa aggaaaaatt ggaacgtcag gaaaaggcaa
     5641 gaaaggatct agctgacgct gtggtgaaag gtcgagagca aaaacaggca aggtcagacg
     5701 atgcaaaacg caagatcctg atcggggctt acttactgaa gaaacatgat aatgacatca
     5761 agaaacttgt ggaacaaaac cccgatttta ttgggtacat tagagaaaat gataagcact
     5821 tattttcata ggcattagta gttataaaaa accctcataa atcgagggtt tcttttttat
     5881 ctgcattact ttttgaatca atgtactttt ttagcttgct atttttcact gcatacagcc
     5941 gcttatactc tttctcaatc aatgcggtca aaatggcttt ttggctctcc cctgctgcgc
     6001 ccaccatgtc cgcaagcata tccgacacac ccttatctat aaacacctcc aaacgttgac
     6061 tatcaaggct tttgcgtttt tcacgatatg ctttttgtcg ttcggcattg gtttttggcg
     6121 gctcttttag catgtcctgt gttttctgat cgttcgggtc tttcatgtct ttaactccca
     6181 tatagtatat catagttaca ggataacatt gtttttagtt acaggataac tttttataaa
     6241 atttatttgt aaaaatttat gctatcctgc aactattttt aaattacagg ataactattt
     6301 aacgttaccc tgtaactata gttatcctgt aacgctaaat taattaccct gtaacatata
     6361 ttttgttatc ctgtaacaac taaatattga aataatacta ccaaagttac cctgtaacga
     6421 aaataactag gttaaaaaat gatcggattt taacattttg cgttgttcca aaagttatca
     6481 acagcctaga acgtcatagg aagcgattac agacacttta tagctatcag catgggaaca
     6541 taagggcagg atgaaatatg ggtcttaaaa cgcaaatggt gaggttttag aggtattttt
     6601 tgaagatgat taaggcggtt tgtttttaaa atttttggcg gctctcaggc tgcttacatt
     6661 ttaaccagtt cagtgaaaag ttctttttca gcaaatttct gtttagcacc atagctaaaa
     6721 cttgcgtgga acatattaag aattgaccga aatgacacaa tctcaattat attttttttg
     6781 aaaagttttc tttatcaaat attttaaatc attgatttat atataagtat acattcattt
     6841 taataattaa tctttattta acaatgattt atctatattc aattgtttaa ttattcttac
     6901 taatattatc tctatatcaa tattttttat ttaaaaacat atgtttagta gtgcttttga
     6961 ttaaagtacc agagggaggg agcagagctg aatgggaaat actcacccta gagcgattct
     7021 taaaaatcac cctaaagtat tcccattcga tgtaccgtcg gtcggtcgct ttcgcatcag
     7081 ggatgacatc actgtatcaa gctgccactg ttatgattac gattgatagc accgcctgaa
     7141 cacgctcata accgccaatt aaatgactac tcattgcgtc cgctactctg ttcagttccc
     7201 ctagtaatag cgtttttccg atgtgtgcta gcgtcactgt acctcatcac ccacacatgg
     7261 acagattgag ttacagacat tggctaaatt tttggggtct atgcttgaca aagcgagtta
     7321 aaaccttaga atttaagaca ggtacattaa gcccctgtgg tgaaatcatt agcggtcgtc
     7381 aaaccttaat gctttcgatt gccgatatgt cagtatcgaa gatctgtacc ccataaatta
     7441 cggtaaagcc ccaagcaatt gcaaggggct tttatctttt ttaacaaaaa aaatttataa
     7501 atcaggattt tataacacta aataatccaa agtacatgag taagtcatga ccactcctgc
     7561 gatgtgtgta gcactctgag tatccgtatt atatcagtga tgtcatatac aaccacataa
     7621 tgcggatgta tcactaactc cctagtatct ttctgtctgc ctgtgcgccc catcagtcga
     7681 ttatcaacaa gtaaatctgt cttttcttca attaaatcat caatttcaat agctgctata
     7741 gggttgcgtt cttcaagata atcatagata tttctacgat cttggcgagc tgtttcagtc
     7801 cactcaatct tcattcttcg ctgccagttt cgccaatgtt gcagcacgtc tagcggcaaa
     7861 ctctgccttt acgtcctcat gtggaatcaa cttgcctgca ttggcttcat ttaagccgat
     7921 ctgtacctgc tgcttgaacc atttgtcata gtctgcggct tcctgttgct tcttaacaaa
     7981 gtcacgcatg aagtcacgga ttagctgcgc ccccgaccga tcaacagtct tagcaaggct
     8041 tgaaaattct tgctttaggt catggtctac ccgaaaggta aaagtcgctt ctgtcatgtt
     8101 tttcactcca atgtgttaca tttgaatgct attgtaacac attaaaaagt taggtcattg
     8161 tcttttttcg gcttaggctg ttcttggtca taacgtggtt taaatgatgg ctgtcgctca
     8221 ttctcaagta aaattttccg tttattaaca gcctgttgca tctgctcata gtaaggcatc
     8281 aactgcggta aaatatcagc aagtgcctgt ctgtctttat ccaggatacg gctcaattgc
     8341 ccgatttttt tcaagtcacg ctcataggct attctgtcgc tgatctgctc gtatttttgc
     8401 tttgaacacc ttatgtactc ttgcatatcg ttacttgccc atgcctgcgc ctgaagacgt
     8461 gtaaacatgg aaatgtctga agcaacatgg tcgctgatat ggtcatactc agcatctttg
     8521 acatagccgt attttgataa atatctttca tagtcctgcc gtttcttttt gtcatcttcc
     8581 tgtttttgtt tagtttctct ttctatctgc tgttgttttc gttgctgttc ctgttgctcc
     8641 tcttttatct ctctgtgata aatggaaagg ctagattgat cattatccca acgtgtgagc
     8701 atgtcctcaa agccgtaata atcagcacta aaccaacctg ttttcacttt cactggtggc
     8761 ggcagtggct ctccgatctc ccttagcagt gctgtagctg ctcgtccctc tttgatcact
     8821 ttatcaagct gcaagcggtc gatattgtct tgcaactgca ggcttcctcg ctcactgact
     8881 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8941 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     9001 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg
     9061 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     9121 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     9181 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     9241 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     9301 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     9361 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     9421 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     9481 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9541 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9601 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9661 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9721 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9781 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9841 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9901 gcttaccatc tggccccagt gctgcaatga taccgcgaga gccacgctca ccggctccag
     9961 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    10021 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    10081 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    10141 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    10201 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    10261 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    10321 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    10381 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    10441 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    10501 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10561 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10621 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10681 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10741 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
    10801 ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat
    10861 aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg cttctagaa
//
