LOCUS       sequence-379835-       10994 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 MoClo plasmid containing an aTC inducible FLAG tagged dCas9 
            for CRISPRi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10994)
  AUTHORS   Brychcy M, Kokodynski A, Lloyd D, Godoy VG
  TITLE     AspFlex: Molecular Tools to Study Gene Expression and Regulation in 
            Acinetobacter baumannii.
  JOURNAL   ACS Synth Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167.
  PUBMED    37587063
REFERENCE   2  (bases 1 to 10994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167."
COMMENT     Sequence Label: pMB1-A-ptet-FLAG-dCas9
FEATURES             Location/Qualifiers
     source          1..10994
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(16..642)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     547..566
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        658..713
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    694..712
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             765..830
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(837..863,864..866,867..3353,3354..3356,3357..4943)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEENPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      4956..4983
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(8942..8959)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9113..9701)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9193..9212)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9872..10663,10664..10732))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10495..10514
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10733..10837)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10905..10923
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(10927..10970)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    10973..10992
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 cgtctcaatc tctatttaag acccactttc acatttaagt tgtttttcta atccgcatat
       61 gatcaattca aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat
      121 agcttgtcgt aataatggcg gcatactatc agtagtaggt gtttcccttt cttctttagc
      181 gacttgatgc tcttgatctt ccaatacgca acctaaagta aaatgcccca cagcgctgag
      241 tgcatataat gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta attgattttc
      301 gagagtttca tactgttttt ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg
      361 atgacttagt aaagcacatc taaaactttt agcgttatta cgtaaaaaat cttgccagct
      421 ttccccttct aaagggcaaa agtgagtatg gtgcctatct aacatctcaa tggctaaggc
      481 gtcgagcaaa gcccgcttat tttttacatg ccaatacaat gtaggctgct ctacacctag
      541 cttctgggcg agtttacggg ttgttaaacc ttcgattccg acctcattaa gcagctctaa
      601 tgcgctgtta atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt
      661 gacactctat cgttgataga gttattttac cactccctat cagtgataga gaaaagaatt
      721 caaaagatct gtactttaac tttaagaagg agatatataa aatggactac aaagaccatg
      781 acggtgatta taaagatcat gacatcgatt acaaggatga cgatgacaag ctccatatgg
      841 ataagaaata ctcaataggc ttagctatcg gcacaaatag cgtcggatgg gcggtgatca
      901 ctgatgaata taaggttccg tctaaaaagt tcaaggttct gggaaataca gaccgccaca
      961 gtatcaaaaa aaatcttata ggggctcttt tatttgacag tggagagaca gcggaagcga
     1021 ctcgtcttaa acggacagct cgtagaaggt atacacgtcg gaagaatcgt atttgttatc
     1081 tacaggagat tttttcaaat gagatggcga aagtagatga tagtttcttt catcgacttg
     1141 aagagtcttt tttggtggaa gaagacaaga agcatgaacg tcatcctatt tttggaaata
     1201 tagtagatga agttgcttat catgagaaat atccaactat ctatcatctg cgaaaaaaat
     1261 tggtagattc tactgataaa gcggatttgc gcttaatcta tttggcctta gcgcatatga
     1321 ttaagtttcg tggtcatttt ttgattgagg gagatttaaa tcctgataat agtgatgtgg
     1381 acaaactatt tatccagttg gtacaaacct acaatcaatt atttgaagaa aaccctatta
     1441 acgcaagtgg agtagatgct aaagcgattc tttctgcacg attgagtaaa tcaagacgat
     1501 tagaaaatct cattgctcag ctccccggtg agaagaaaaa tggcttattt gggaatctca
     1561 ttgctttgtc attgggtttg acccctaatt ttaaatcaaa ttttgatttg gcagaagatg
     1621 ctaaattaca gctttcaaaa gatacttacg atgatgattt agataattta ttggcgcaaa
     1681 ttggagatca atatgctgat ttgtttttgg cagctaagaa tttatcagat gctattttac
     1741 tttcagatat cctaagagta aatactgaaa taactaaggc tcccctatca gcttcaatga
     1801 ttaaacgcta cgatgaacat catcaagact tgactctttt aaaagcttta gttcgacaac
     1861 aacttccaga aaagtataaa gaaatctttt ttgatcaatc aaaaaacgga tatgcaggtt
     1921 atattgatgg gggagctagc caagaagaat tttataaatt tatcaaacca attttagaaa
     1981 aaatggatgg tactgaggaa ttattggtga aactaaatcg tgaagatttg ctgcgcaagc
     2041 aacggacctt tgacaacggc tctattcccc atcaaattca cttgggtgag ctgcatgcta
     2101 ttttgagaag acaagaagac ttttatccat ttttaaaaga caatcgtgag aagattgaaa
     2161 aaatcttgac ttttcgaatt ccttattatg ttggtccatt ggcgcgtggc aatagtcgtt
     2221 ttgcatggat gactcggaag tctgaagaaa caattacccc atggaatttt gaagaagttg
     2281 tcgataaagg tgcttcagct caatcattta ttgaacgcat gacaaacttt gataaaaatc
     2341 ttccaaatga aaaagtacta ccaaaacata gtttgcttta tgagtatttt acggtttata
     2401 acgaattgac aaaggtcaaa tatgttactg aaggaatgcg aaaaccagca tttctttcag
     2461 gtgaacagaa gaaagccatt gttgatttac tcttcaaaac aaatcgaaaa gtaaccgtta
     2521 agcaattaaa agaagattat ttcaaaaaaa tagaatgttt tgatagtgtt gaaatttcag
     2581 gagttgaaga tagatttaat gcttcattag gtacctacca tgatttgcta aaaattatta
     2641 aagataaaga ttttttggat aatgaagaaa atgaagatat cttagaggat attgttttaa
     2701 cattgacctt atttgaagat agggagatga ttgaggaaag acttaaaaca tatgctcacc
     2761 tctttgatga taaggtgatg aaacagctta aacgtcgccg ttatactggt tggggacgtt
     2821 tgtctcgaaa attgattaat ggtattaggg ataagcaatc tggcaaaaca atattagatt
     2881 ttttgaaatc agatggtttt gccaatcgca attttatgca gctgatccat gatgatagtt
     2941 tgacatttaa agaagacatt caaaaagcac aagtgtctgg acaaggcgat agtttacatg
     3001 aacatattgc aaatttagct ggtagccctg ctattaaaaa aggtatttta cagactgtaa
     3061 aagttgttga tgaattggtc aaagtaatgg ggcggcataa gccagaaaat atcgttattg
     3121 aaatggcacg tgaaaatcag acaactcaaa agggccagaa aaattcgcga gagcgtatga
     3181 aacgaatcga agaaggtatc aaagaattag gaagtcagat tcttaaagag catcctgttg
     3241 aaaatactca attgcaaaat gaaaagctct atctctatta tctccaaaat ggaagagaca
     3301 tgtatgtgga ccaagaatta gatattaatc gtttaagtga ttatgatgtc gatgccattg
     3361 ttccacaaag tttccttaaa gacgattcaa tagacaataa ggtcttaacg cgttctgata
     3421 aaaatcgtgg taaatcggat aacgttccaa gtgaagaagt agtcaaaaag atgaaaaact
     3481 attggagaca acttctaaac gccaagttaa tcactcaacg taagtttgat aatttaacga
     3541 aagctgaacg tggaggtttg agtgaacttg ataaagctgg ttttatcaaa cgccaattgg
     3601 ttgaaactcg ccaaatcact aagcatgtgg cacaaatttt ggatagtcgc atgaatacta
     3661 aatacgatga aaatgataaa cttattcgag aggttaaagt gattacctta aaatctaaat
     3721 tagtttctga cttccgaaaa gatttccaat tctataaagt acgtgagatt aacaattacc
     3781 atcatgccca tgatgcgtat ctaaatgccg tcgttggaac tgctttgatt aagaaatatc
     3841 caaaacttga atcggagttt gtctatggtg attataaagt ttatgatgtt cgtaaaatga
     3901 ttgctaagtc tgagcaagaa ataggcaaag caaccgcaaa atatttcttt tactctaata
     3961 tcatgaactt cttcaaaaca gaaattacac ttgcaaatgg agagattcgc aaacgccctc
     4021 taatcgaaac taatggggaa actggagaaa ttgtctggga taaagggcga gattttgcca
     4081 cagtgcgcaa agtattgtcc atgccccaag tcaatattgt caagaaaaca gaagtacaga
     4141 caggcggatt ctccaaggag tcaattttac caaaaagaaa ttcggacaag cttattgctc
     4201 gtaaaaaaga ctgggatcca aaaaaatatg gtggttttga tagtccaacg gtagcttatt
     4261 cagtcctagt ggttgctaag gtggaaaaag ggaaatcgaa gaagttaaaa tccgttaaag
     4321 agttactagg gatcacaatt atggaaagaa gttcctttga agaaaatccg attgactttt
     4381 tagaagctaa aggatataag gaagttaaaa aagacttaat cattaaacta cctaaatata
     4441 gtctttttga gttagaaaac ggtcgtaaac ggatgctggc tagtgccgga gaattacaaa
     4501 aaggaaatga gctggctctg ccaagcaaat atgtgaattt tttatattta gctagtcatt
     4561 atgaaaagtt gaagggtagt ccagaagata acgaacaaaa acaattgttt gtggagcagc
     4621 ataagcatta tttagatgag attattgagc aaatcagtga attttctaag cgtgttattt
     4681 tagcagatgc caatttagat aaagttctta gtgcatataa caaacataga gacaaaccaa
     4741 tacgtgaaca agcagaaaat attattcatt tatttacgtt gacgaatctt ggagctcccg
     4801 ctgcttttaa atattttgat acaacaattg atcgtaaacg atatacgtct acaaaagaag
     4861 ttttagatgc cactcttatc catcaatcca tcactggtct ttatgaaaca cgcattgatt
     4921 tgagtcagct aggaggtgac taactcgatc acactggctc accttcgggt gggcctttct
     4981 gcgtttatat gtttgccaga gacgctgcag ttaacaagtt gcctgacgct gtgtcttatc
     5041 cagttcatgc ggcaatgcta tctctacttc ctttgctaat cttgcctcct gtttgatgtc
     5101 accgttcttt tttagttcgg attgctctac ccgattccat agggtttgac gatctaacat
     5161 gtcaggactt gccccagttg gggcaaaaat ttgggtgtat tcaatgcctg tttttttggt
     5221 gtagtcctgc tcttttccgt acgtatcaca gtacaatttt tcgcctgctc ggtatgctgc
     5281 acatgccacg attgagcgac catctgacct cgaaatgttc tgcatttcac aatggtaaat
     5341 tgccattttc atcacctgtt tattaacagc cgccccaagt tttgggcggt tggggtgtcg
     5401 gggtttccct gaccgtactt gcaaaatttg gcgtagcaaa aattttgcgt aagtgcgccc
     5461 ttcgggaact ctgcgaggct aaaagcaaaa gcaagagcaa taaggctttg actttgtttt
     5521 ttgattttgc tcgctgcgct cggatcttga tgtaagtcga gtttttgaag tagaacactt
     5581 ttcacattga tgatggttca tacttcggaa aataggttga gacacaggca ttaaaaatgg
     5641 tcaaaaaaac agctatggaa ttgggtcaaa tgctcgatga agaaaaggaa aaattggaac
     5701 gtcaggaaaa ggcaagaaag gatctagctg acgctgtggt gaaaggtcga gagcaaaaac
     5761 aggcaaggtc agacgatgca aaacgcaaga tcctgatcgg ggcttactta ctgaagaaac
     5821 atgataatga catcaagaaa cttgtggaac aaaaccccga ttttattggg tacattagag
     5881 aaaatgataa gcacttattt tcataggcat tagtagttat aaaaaaccct cataaatcga
     5941 gggtttcttt tttatctgca ttactttttg aatcaatgta cttttttagc ttgctatttt
     6001 tcactgcata cagccgctta tactctttct caatcaatgc ggtcaaaatg gctttttggc
     6061 tctcccctgc tgcgcccacc atgtccgcaa gcatatccga cacaccctta tctataaaca
     6121 cctccaaacg ttgactatca aggcttttgc gtttttcacg atatgctttt tgtcgttcgg
     6181 cattggtttt tggcggctct tttagcatgt cctgtgtttt ctgatcgttc gggtctttca
     6241 tgtctttaac tcccatatag tatatcatag ttacaggata acattgtttt tagttacagg
     6301 ataacttttt ataaaattta tttgtaaaaa tttatgctat cctgcaacta tttttaaatt
     6361 acaggataac tatttaacgt taccctgtaa ctatagttat cctgtaacgc taaattaatt
     6421 accctgtaac atatattttg ttatcctgta acaactaaat attgaaataa tactaccaaa
     6481 gttaccctgt aacgaaaata actaggttaa aaaatgatcg gattttaaca ttttgcgttg
     6541 ttccaaaagt tatcaacagc ctagaacgtc ataggaagcg attacagaca ctttatagct
     6601 atcagcatgg gaacataagg gcaggatgaa atatgggtct taaaacgcaa atggtgaggt
     6661 tttagaggta ttttttgaag atgattaagg cggtttgttt ttaaaatttt tggcggctct
     6721 caggctgctt acattttaac cagttcagtg aaaagttctt tttcagcaaa tttctgttta
     6781 gcaccatagc taaaacttgc gtggaacata ttaagaattg accgaaatga cacaatctca
     6841 attatatttt ttttgaaaag ttttctttat caaatatttt aaatcattga tttatatata
     6901 agtatacatt cattttaata attaatcttt atttaacaat gatttatcta tattcaattg
     6961 tttaattatt cttactaata ttatctctat atcaatattt tttatttaaa aacatatgtt
     7021 tagtagtgct tttgattaaa gtaccagagg gagggagcag agctgaatgg gaaatactca
     7081 ccctagagcg attcttaaaa atcaccctaa agtattccca ttcgatgtac cgtcggtcgg
     7141 tcgctttcgc atcagggatg acatcactgt atcaagctgc cactgttatg attacgattg
     7201 atagcaccgc ctgaacacgc tcataaccgc caattaaatg actactcatt gcgtccgcta
     7261 ctctgttcag ttcccctagt aatagcgttt ttccgatgtg tgctagcgtc actgtacctc
     7321 atcacccaca catggacaga ttgagttaca gacattggct aaatttttgg ggtctatgct
     7381 tgacaaagcg agttaaaacc ttagaattta agacaggtac attaagcccc tgtggtgaaa
     7441 tcattagcgg tcgtcaaacc ttaatgcttt cgattgccga tatgtcagta tcgaagatct
     7501 gtaccccata aattacggta aagccccaag caattgcaag gggcttttat cttttttaac
     7561 aaaaaaaatt tataaatcag gattttataa cactaaataa tccaaagtac atgagtaagt
     7621 catgaccact cctgcgatgt gtgtagcact ctgagtatcc gtattatatc agtgatgtca
     7681 tatacaacca cataatgcgg atgtatcact aactccctag tatctttctg tctgcctgtg
     7741 cgccccatca gtcgattatc aacaagtaaa tctgtctttt cttcaattaa atcatcaatt
     7801 tcaatagctg ctatagggtt gcgttcttca agataatcat agatatttct acgatcttgg
     7861 cgagctgttt cagtccactc aatcttcatt cttcgctgcc agtttcgcca atgttgcagc
     7921 acgtctagcg gcaaactctg cctttacgtc ctcatgtgga atcaacttgc ctgcattggc
     7981 ttcatttaag ccgatctgta cctgctgctt gaaccatttg tcatagtctg cggcttcctg
     8041 ttgcttctta acaaagtcac gcatgaagtc acggattagc tgcgcccccg accgatcaac
     8101 agtcttagca aggcttgaaa attcttgctt taggtcatgg tctacccgaa aggtaaaagt
     8161 cgcttctgtc atgtttttca ctccaatgtg ttacatttga atgctattgt aacacattaa
     8221 aaagttaggt cattgtcttt tttcggctta ggctgttctt ggtcataacg tggtttaaat
     8281 gatggctgtc gctcattctc aagtaaaatt ttccgtttat taacagcctg ttgcatctgc
     8341 tcatagtaag gcatcaactg cggtaaaata tcagcaagtg cctgtctgtc tttatccagg
     8401 atacggctca attgcccgat ttttttcaag tcacgctcat aggctattct gtcgctgatc
     8461 tgctcgtatt tttgctttga acaccttatg tactcttgca tatcgttact tgcccatgcc
     8521 tgcgcctgaa gacgtgtaaa catggaaatg tctgaagcaa catggtcgct gatatggtca
     8581 tactcagcat ctttgacata gccgtatttt gataaatatc tttcatagtc ctgccgtttc
     8641 tttttgtcat cttcctgttt ttgtttagtt tctctttcta tctgctgttg ttttcgttgc
     8701 tgttcctgtt gctcctcttt tatctctctg tgataaatgg aaaggctaga ttgatcatta
     8761 tcccaacgtg tgagcatgtc ctcaaagccg taataatcag cactaaacca acctgttttc
     8821 actttcactg gtggcggcag tggctctccg atctccctta gcagtgctgt agctgctcgt
     8881 ccctctttga tcactttatc aagctgcaag cggtcgatat tgtcttgcaa ctgcaggctt
     8941 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     9001 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     9061 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca
     9121 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     9181 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     9241 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     9301 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     9361 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     9421 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     9481 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     9541 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     9601 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     9661 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     9721 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     9781 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     9841 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     9901 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     9961 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagagccac
    10021 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    10081 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    10141 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    10201 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    10261 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    10321 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    10381 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    10441 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    10501 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    10561 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    10621 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    10681 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    10741 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    10801 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    10861 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
    10921 ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgc
    10981 ggccgcttct agaa
//
