LOCUS       sequence-379939-        5600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV transfer plasmid for neuronal expression of axon-targeted 
            jGCaMP8f..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Grodem S, Nymoen I, Vatne GH, Rogge FS, Bjornsdottir V, Lensjo KK, 
            Fyhn M
  TITLE     An updated suite of viral vectors for in vivo calcium imaging using 
            intracerebral and retro-orbital injections in male mice.
  JOURNAL   Nat Commun. 2023 Feb 4;14(1):608. doi: 10.1038/s41467-023-36324-3.
  PUBMED    36739289
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-02-4"; volume: "14(1):608. doi"; pages: " 
            10.1038/s41467-023-36324-3"
COMMENT     Sequence Label: AAV-hSyn-Axon-jGCaMP8f
FEATURES             Location/Qualifiers
     source          1..5600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        211..658
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             759..776
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     1058..1079
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1185..1206)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1446..1465)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2037..2625
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2090..2110)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2508..2519)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2628..2644)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2629..2645)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    2674..2795
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2685..2704)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2739..2758
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   2957..3086
                     /label=AAV2 ITR
     rep_origin      complement(3323..3911)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3403..3422)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4082..4873,4874..4942))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4705..4724
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4943..5047)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5073..5528)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5210..5231)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5422..5441
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   join(5600,1..129)
                     /label=AAV2 ITR
ORIGIN
        1 tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc cgggcgtcgg gcgacctttg
       61 gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta
      121 ggggttcctt gtagttaatg attaacccgc catgctactt atctacgtag ccatgctcta
      181 ggaagatctc tgcagagggc cctgcgtatg agtgcaagtg ggttttagga ccaggatgag
      241 gcggggtggg ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc
      301 cattccccaa attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg
      361 cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc
      421 gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac
      481 tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca
      541 cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc
      601 agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc
      661 gaattcaagc tgctagcaag gatccacccg ccaccatgct gtgctgtatg agaagaacca
      721 aacaggttga aaagaatgat gaggaccaaa agattatgca tcatcaccat catcacacgc
      781 gtcgcaagaa gaccttcaag gaggtggcca ccgccgtgaa gatcatcgcc cgcctgatgg
      841 gcctgaagat caacgtctat atcaaggccg acaagcagaa gaacggcatc aaggcgaact
      901 tccacatccg ccacaacatc gaggacggcg gcgtgcagct cgcctaccac taccagcaga
      961 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcgtggagt
     1021 ccaaactttc gaaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     1081 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggcggtacc ggagggagca
     1141 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     1201 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg tgagggcgat gccacctacg
     1261 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     1321 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     1381 agcacgactt cttcaagtcc gccatgcccg aaggctacat ccaggagcgc accatcttct
     1441 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     1501 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     1561 agctggagta caacctgccg gaccaactga ctgaagagca gatcgcagaa tttaaagagg
     1621 ctttctccct atttgacaag gacggggatg ggacaataac aaccaaggag ctggggacgg
     1681 tgatgcggtc tctcggactg aaccccacag aagcagagct gcaggacatg atcaatgaag
     1741 tagatgccga cggtgacggc acaatcgact tccctgagtt cctgacaatg atggcaagaa
     1801 aaatgaaata cagggacacg gaagaagaaa ttagagaagc gttcggtgtg tttgataagg
     1861 atggcaatgg ctacatcagt gcagcagagc ttcgccacgt gatgacaaac cttggagaga
     1921 agttaacaga tgaagaggtt gatgaaatga tcagggaagc agacatcgat ggggatggtc
     1981 aggtaaacta cgaagagttt gtacaaatga tgacagcgaa gtaaaagctt atcgataatc
     2041 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     2101 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     2161 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     2221 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     2281 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     2341 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     2401 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     2461 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     2521 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     2581 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     2641 ctcgacccgg gcggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa
     2701 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     2761 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     2821 tgtttcaggt tcagggggag atgtgggagg ttttttaaag caagtaaaac ctctacaaat
     2881 gtggtaaaat cgataaggat cttcctagag catggctacg tagataagta gcatggcggg
     2941 ttaatcatta actacaagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     3001 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     3061 gcctcagtga gcgagcgagc gcgcagctgc attaatgaat cggccaacgc gcggggagag
     3121 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     3181 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3241 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3301 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3361 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3421 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3481 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3541 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3601 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3661 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3721 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3781 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3841 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3901 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3961 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     4021 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     4081 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     4141 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     4201 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     4261 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     4321 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     4381 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     4441 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     4501 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4561 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4621 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4681 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4741 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4801 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4861 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4921 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4981 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     5041 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
     5101 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     5161 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     5221 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     5281 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     5341 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     5401 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     5461 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     5521 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     5581 ctcttcgcta ttacgccagc
//
