LOCUS       sequence-380037-        3702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway entry clone for expression of the redox probe 
            GLRX-roGFP2. Parton lab clone KQF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Wu Y, Lim YW, Stroud DA, Martel N, Hall TE, Lo HP, Ferguson C, Ryan 
            MT, McMahon KA, Parton RG
  TITLE     Caveolae sense oxidative stress through membrane lipid peroxidation 
            and cytosolic release of CAVIN1 to regulate NRF2.
  JOURNAL   Dev Cell. 2023 Mar 13;58(5):376-397.e4. doi: 
            10.1016/j.devcel.2023.02.004. Epub 2023 Feb 28.
  PUBMED    36858041
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2023.02.004"; journalName: "Dev Cell"; date: 
            "2023-03-13- 13"; volume: "58"; issue: "5"; pages: "376-397.e4"
COMMENT     Sequence Label: pME-p3E-GLRX-roGFP2
FEATURES             Location/Qualifiers
     source          1..3702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..56
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     290..307
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(466..493)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(585..671)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     720..742
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     734..751
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     735..751
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    766..865
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      866..875
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2009..2104)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2141..2160)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2142..2160)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2165..2181)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2165..2181)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2283..2302)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2294..3103
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2361..2380)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(3250..3702,1..136)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
       61 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      121 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      181 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      241 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      301 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
      361 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
      421 aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc
      481 aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac
      541 cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg
      601 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt
      661 cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc
      721 ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggcccaaat aatgatttta
      781 ttttgactga tagtgacctg ttcgttgcaa cacattgatg agcaatgctt ttttataatg
      841 ccaactttgt acaaaaaagc aggctgccac catggcccag gaatttgtta attgcaagat
      901 acaacccggc aaggttgtag tgtttatcaa gccgacctgt ccttactgcc gacgtgcgca
      961 agaaattctt tcacagctgc caataaagca ggggttgttg gagttcgttg atattacagc
     1021 aactaaccac accaatgaga tccaagacta tttgcaacaa cttaccggtg cgcgaacagt
     1081 tccaagggtc tttatcggaa aggattgtat aggcggctgc tctgaccttg tctctctgca
     1141 acaatctggc gagctcctga cgaggttgaa acaaataggc gcactgcaga cttctggagg
     1201 tagtggaggg ggtggttcag gcggtggcgg ttctggtggt ggaggtagcg ggggcggcgg
     1261 cagcggagga ggcggatccg ggggagagtt tgtgagtaag ggtgaggaac tctttactgg
     1321 tgttgtcccg atcctggtgg agctcgatgg ggatgtgaac gggcacaaat tctccgtcag
     1381 cggggagggc gaaggtgacg ccacgtacgg gaaattgacg cttaaattta ttagtaccac
     1441 aggaaagctg ccagtcccgt ggccaaccct cgtaacgacc cttacgtacg gagtgcaatg
     1501 cttttccagg taccctgatc acatgaagca acacgacttc ttcaaatccg ccatgcctga
     1561 aggttatgtt caagaacgga ccatcttttt caaagacgac ggcaactata agacaagagc
     1621 agaggtcaag tttgagggtg atacacttgt caatagaatc gagctgaaag ggattgactt
     1681 caaggaggat ggtaatatcc tggggcataa gctcgagtac aactacaact gccataacgt
     1741 ctacattatg gccgataaac agaagaacgg gattaaagta aatttcaaga ttcgtcataa
     1801 cattgaagac ggcagcgttc aacttgcaga tcattaccag caaaacacgc ccattggaga
     1861 cgggccggtt ctgcttcccg acaaccatta cttgagcacg tgtagtgccc tctctaagga
     1921 cccgaacgag aagcgggatc acatggttct tcttgagttt gtaacagcag ccgggataac
     1981 cctgggtatg gatgagttgt acaaataaca actttattat acaaagttgg cattataaaa
     2041 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     2101 atttggagct ccatggtagc gttaacgcgg ccgcgatatc ccctatagtg agtcgtatta
     2161 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     2221 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     2281 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     2341 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     2401 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     2461 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     2521 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     2581 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     2641 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     2701 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     2761 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     2821 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     2881 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     2941 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     3001 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     3061 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     3121 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     3181 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3241 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3301 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3361 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3421 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3481 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3541 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3601 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3661 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc ag
//
