LOCUS       sequence-38020-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Kelly TJ, Souza AL, Clish CB, Puigserver P
  TITLE     A hypoxia-induced positive feedback loop promotes hypoxia-inducible 
            factor 1alpha stability through miR-210 suppression of 
            glycerol-3-phosphate dehydrogenase 1-like.
  JOURNAL   Mol Cell Biol. 2011 Jul;31(13):2696-706. Epub 2011 May 9.
  PUBMED    21555452
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2011-07"; volume: "31(13)"; pages: "2696-706. Epub 
            2011 May 9"
COMMENT     Sequence Label: pcDNA-FLAG-GPD1
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             88..111
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agagctctct ggctactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct taccatggac tacaaggacg acgatgacaa gggatccgct
      121 ggcaagaaag tctgcattgt cggctctggc aactggggct cagccattgc caagatcgtg
      181 ggtagcaatg caggccggct ggcacacttt gacccacggg tgaccatgtg ggtgtttgag
      241 gaagacatcg ggggcagaaa gctaactgag atcatcaaca ctcagcacga gaatgttaaa
      301 tacctgccag ggcacaagct gccccccaat gtggtggcca tcccagacgt ggtccaggct
      361 gcaacaggcg ctgacatcct ggtttttgtg gtaccccatc agttcattgg caagatctgt
      421 gaccagctca agggccactt gaaggccaat actattggca tatctcttat taagggggta
      481 gacgagggcc ccaacgggct gaagctcatt tctgaagtga ttggggagcg ccttggcatt
      541 cccatgagcg tgctgatggg ggccaacatt gccagcgagg tggctgagga gaagttctgt
      601 gagacgacca tcggctgcaa ggacccggcc cagggacagc tcctgaagga cctgatgcag
      661 acacccaact ttcgcatcac tgtggtacaa gaggtggaca cagtggagat ctgtggggcc
      721 ttgaagaata tagtggccgt tggggctggc ttctgtgatg ggcttggctt cggtgacaac
      781 accaaggcgg cggtgatccg gttggggctc atggagatga tcgccttcgc caagctcttc
      841 tgcagtggca ctgtgtcctc ggccaccttc ctggagagct gcggggtcgc a
//
