LOCUS       sequence-380212-        3164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid to produce ISH probe for mMyh8 gene with UTR sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Tanji M, Wada K, Sakamoto K, Ono Y, Inui M
  TITLE     Digoxigenin-labeled RNA probes for untranslated regions enable the 
            isoform-specific gene expression analysis of myosin heavy chains in 
            whole-mount in situ hybridization.
  JOURNAL   Dev Growth Differ. 2022 Dec 14. doi: 10.1111/dgd.12832.
  PUBMED    36517457
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Growth 
            Differ. 2022 Dec 14. doi: 10.1111/dgd.12832."
COMMENT     Sequence Label: pGEMT-mMyh8UTR
FEATURES             Location/Qualifiers
     source          1..3164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      375..963
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     864..883
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1117..1134
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1251..1272
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1287..1292,1293..1310,1311..1317)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1325..1341
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1330..1352
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1349..1365
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1349..1365
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1383..1401
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1383..1400
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1644..1653
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1648..1657
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1687..1706)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1688..1706)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1713..1730)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1713..1729)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1722..1744)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1870..2325
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1957..1976)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2167..2188
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2403..2507
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2508..2576,2577..3164,1..204)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2726..2745)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
       61 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
      121 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
      181 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
      241 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
      301 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
      361 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
      421 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
      481 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
      541 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
      601 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
      661 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
      721 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
      781 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      841 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      901 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      961 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     1021 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     1081 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     1141 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     1201 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     1261 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     1321 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     1381 ctatttaggt gacactatag aatactcaag ctatgcatcc aacgcgttgg gagctctccc
     1441 atatggtcga cctgcaggcg gccgcgaatt cactagtgat tgatttacca atgccttgta
     1501 gtttttattt agtcagcagt aggagaaaag taaacccaga gaggcaagtg acccagcaag
     1561 cgacccaaag cagcccctcc tgtgctttcc ttcagcccct tggcagcctc cttcctctgc
     1621 aagatgtgaa tcgaattccc gcggccgcca tggcggccgg gagcatgcga cgtcgggccc
     1681 aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac
     1741 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     1801 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     1861 ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     1921 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     1981 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     2041 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     2101 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     2161 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     2221 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     2281 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt
     2341 tctccttacg catctgtgcg gtatttcaca ccgcatcagg tggcactttt cggggaaatg
     2401 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     2461 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     2521 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     2581 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     2641 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     2701 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     2761 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     2821 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     2881 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     2941 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     3001 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     3061 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     3121 taatagactg gatggaggcg gataaagttg caggaccact tctg
//
