LOCUS       sequence-380220-        9937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycyline-inducible (TetOn) human Cdt1-Delta-PIP (C-terminal 
            mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Ratnayeke N, Baris Y, Chung M, Yeeles JTP, Meyer T
  TITLE     CDT1 inhibits CMG helicase in early S phase to separate origin 
            licensing from DNA synthesis.
  JOURNAL   Mol Cell. 2023 Jan 5;83(1):26-42.e13. doi: 
            10.1016/j.molcel.2022.12.004.
  PUBMED    36608667
REFERENCE   2  (bases 1 to 9937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2022.12"; journalName: "Mol Cell"; date: 
            "2023-01-5- 5"; volume: "83"; issue: "1"; pages: "26-42.e13"
COMMENT     Sequence Label: pCW-Cdt1-Delta-PIP-mCherry-Puro
FEATURES             Location/Qualifiers
     source          1..9937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             582..854
                     /codon_start=1
                     /product="degron consisting of residues 30-120 of human 
                     Cdt1 (Zielke and Edgar, 2015)"
                     /label=Cdt1 (30-120)
                     /translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
                     DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
     CDS             2157..2867
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2302..2320)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2570..2589
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        2879..3389
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3308..3326
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3399..3995
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3399..3418)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3895..3915
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3996..4049
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4050..4667,4668..4673,4674..4709,4710..4712,4713..4748,
                     4749..4751,4752..4787,4788..4796)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4836..4856)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5254..5265)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5470..5703
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5775..5896
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5812..5831)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5866..5885
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5936..6071
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6071..6142
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6143..6211,6212..7003)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6361..6380)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7174..7762
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7663..7682
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7916..7933
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8050..8071
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8086..8091,8092..8109,8110..8116)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8124..8140
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8129..8151
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8148..8164
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8148..8164
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8183..8203
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8185..8203
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8231..8457
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8458..8638
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8685..8810
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9303..9536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9721..9765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcgccaccat gatcgcgccg cccaagctgg cctgccgcac ccccagcccc gccaggcccg
      601 cactccgcgc cccggcctcc gctaccagtg gcagccgcaa gcgcgcccgc ccgcccgccg
      661 cccccggacg cgaccaggcc aggccaccgg cccgcaggag actgcggctg tcggtggacg
      721 aggtttccag ccccagtacc cccgaggccc cagacatccc agcctgccct tctccgggcc
      781 agaagataaa gaaatccacc ccggcagcag gtcagccgcc ccacctgaca tccgcgcagg
      841 accaggacac catctctgag cttgcgtcat gcctgcaacg ggcccgggag ctgggggcaa
      901 gagtccgggc gctgaaggcc agtgcccagg atgctgggga gtcctgcacc ccagaggccg
      961 agggccgccc tgaggagcca tgtggcgaga aggcgcccgc ctaccagcgc ttccatgccc
     1021 tggcccagcc cggcctgccg ggactcgtgc tgccctacaa gtaccaggtg ctggcggaga
     1081 tgttccgcag catggacacc atcgtgggca tgctccacaa ccgctccgag acgcccacct
     1141 ttgccaaggt ccagcggggc gtccaggaca tgatgcgtag gcgttttgag gagcgcaatg
     1201 ttggccagat caaaaccgtg tacccggcct cctaccgctt ccgccaggag cgcagtgtcc
     1261 ccaccttcaa ggatggcacc aggaggtcag attaccagct caccatcgag ccactgctgg
     1321 agcaggaggc tgacggagca gccccccagc tcacggcctc gcgcctcctg cagcgacggc
     1381 agatcttcag ccagaagctg gtggagcatg tcaaggagca ccacaaggcc ttcctggcct
     1441 ccctgagccc cgccatggtg gtgccggagg accagctgac ccgctggcac ccgcgcttca
     1501 acgtggatga agtacccgac atcgagccgg ccgcgctgcc ccagccaccc gccacggaga
     1561 agctcaccac tgctcaggag gtgctggccc gggcccgcaa cctgatttca cccaggatgg
     1621 agaaggcctt gagtcaattg gccctgcgct ctgctgcgcc cagcagcccc gggtctccca
     1681 ggccagcact gccggctacc ccaccagcca ccccgcctgc agcctctccc agtgctctga
     1741 agggggtgtc ccaggatctg ctggagcgga tccgagccaa ggaggcacag aagcagctgg
     1801 cacagatgac gcggtgcccg gagcaggagc agcggctgca gcgcttagaa cggctgcctg
     1861 agctggcccg cgtgctgcgg agcgtctttg tgtccgaacg caagcctgcg ctcagcatgg
     1921 aggtggcctg tgccaggatg gtgggcagct gttgtactat catgagccct ggggaaatgg
     1981 agaagcacct gctgctcctc tccgagctgc tgccggactg gctcagcctc caccgcatcc
     2041 gcaccgacac ctacgtcaag ctggacaagg ccgcggacct cgcccacatc actgcacgcc
     2101 tggcccacca gacacgtgct gaggaggggc tgggtgcagg aagcgctggg ggatccatgg
     2161 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     2221 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     2281 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     2341 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
     2401 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     2461 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     2521 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     2581 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     2641 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     2701 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     2761 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     2821 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtgagaa ttcgatccgg
     2881 ggttggggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg
     2941 ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg
     3001 ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg
     3061 ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg
     3121 aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc
     3181 gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg
     3241 gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc
     3301 ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg
     3361 ttgaccgaat caccgacctc tctccccagc aattcaccat gaccgagtac aagcccacgg
     3421 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     3481 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     3541 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     3601 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     3661 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     3721 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     3781 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     3841 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     3901 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     3961 cgcgcacctg gtgcatgacc cgcaagcccg gtgccgaagg tagaggttct ctcctcactt
     4021 gtggtgatgt tgaagaaaac cctggtccaa tgtctagact ggacaagagc aaagtcataa
     4081 acggagctct ggaattactc aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg
     4141 ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc
     4201 tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg
     4261 aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc tgtgctctcc
     4321 tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg
     4381 aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac
     4441 tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc
     4501 atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga
     4561 gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg
     4621 aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccgaccgacg
     4681 cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg
     4741 atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaactaa
     4801 gtaaggatcg atccaagata tcgtattctt aactatgttg ctccttttac gctatgtgga
     4861 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     4921 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     4981 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     5041 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     5101 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     5161 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     5221 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     5281 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     5341 agtcggatct ccctttgggc cgcctccccg cctgtttcgc ctcggcgtcc ggactagagg
     5401 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     5461 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     5521 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     5581 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     5641 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     5701 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     5761 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5821 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5881 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     5941 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6001 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     6061 ttttggaggc ctttcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     6121 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     6181 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     6241 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     6301 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     6361 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     6421 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     6481 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     6541 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     6601 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     6661 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     6721 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     6781 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     6841 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     6901 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     6961 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     7021 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     7081 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     7141 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7201 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7261 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7321 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7381 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7441 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7501 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7561 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7621 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7681 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7741 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     7801 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     7861 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     7921 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     7981 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     8041 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     8101 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     8161 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     8221 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
     8281 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
     8341 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
     8401 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
     8461 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8521 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8581 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8641 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     8701 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     8761 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     8821 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     8881 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     8941 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     9001 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     9061 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     9121 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     9181 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     9241 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     9301 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     9361 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     9421 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     9481 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     9541 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     9601 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     9661 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     9721 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     9781 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     9841 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     9901 ttatcgtttc agacccacct cccaaccccg aggggac
//
