LOCUS       sequence-38025-e       11057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11057)
  AUTHORS   Yu CR, Power J, Barnea G, O'Donnell S, Brown HE, Osborne J, Axel R, 
            Gogos JA
  TITLE     Spontaneous neural activity is required for the establishment and 
            maintenance of the olfactory sensory map.
  JOURNAL   Neuron. 2004 May 27;42(4):553-66.
  PUBMED    15157418
REFERENCE   2  (bases 1 to 11057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2004-05-27"; volume: "42(4)"; pages: "553-66"
COMMENT     Sequence Label: pTetO-Kir2.1-IRES-TLZ
FEATURES             Location/Qualifiers
     source          1..11057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(94..113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     304..325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    join(723..741,742..764,765..783,784..806,807..825,826..848,
                     849..867,868..890,891..909,910..932,933..951,952..974,
                     975..993)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        1026..1064
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1061..1085
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1220..1449
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     1432..1451
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(2864..3437,3438..3440,3441..3449)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3031..3048)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             4659..7706
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(7726..7742)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7726..7742)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(7752..7770)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7753..7770)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          7854..7919
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8048..8068
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8340..8474
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8377..8396)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8431..8450
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(8872..8890)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8911..8927)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8911..8927)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8924..8946)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8935..8951
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8959..8965,8966..8983,8984..8989))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9004..9025
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9142..9159)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9313..9901)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9393..9412)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10072..10863,10864..10932))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10695..10714
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10933..11037)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggcgattg
      661 gactcgttta gacggcgcgc cgtttaaacg gcgcggcggc cgccaactct cgagtttacc
      721 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
      781 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
      841 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
      901 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
      961 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg
     1021 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     1081 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cggggaccga
     1141 tccagcctcc gcggccccga attcaccacg tggccgcatc gattctagaa ttcgctgtct
     1201 gcgagggcca gctgttgggg tgagtactcc ctctcaaaag cgggcatgac ttctgcgcta
     1261 agattgtcag tttccaaaaa cgaggaggat ttgatattca cctggcccgc ggtgatgcct
     1321 ttgagggtgg ccgcgtccat ctggtcagaa aagacaatct ttttgttgtc aagcttgagg
     1381 tgtggcaggc ttgagatctg gccatacact tgagtgacaa tgacatccac tttgcctttc
     1441 tctccacagg tgtccactcc caggtccaac tgcagcccaa gcggatcctg cggatcgagt
     1501 taattaatcg cgatgtcgag accatgggca gtgtgagaac caaccgctac agcatcgtct
     1561 cttcggagga agatggcatg aagctggcca ctatggcagt tgccaatggc tttgggaatg
     1621 gcaagagtaa agtccatacc cgacaacagt gcaggagccg ctttgtgaag aaagatggtc
     1681 attgcaatgt tcagtttatc aacgtgggtg agaagggaca gaggtacctg gcagacatct
     1741 ttactacctg tgtcgacatc cgctggaggt ggatgctggt tatcttctgt cttgccttcg
     1801 tgctctcctg gctgttcttt ggctgtgtgt tttggttgat agccctgctc catggggatc
     1861 tagatacttc taaagtgagc aaagcatgcg tgtcggaggt caacagcttc acggctgcct
     1921 tcctcttctc catcgagacc cagacaacca ttggctatgg tttcaggtgt gtgacagacg
     1981 agtgcccaat tgctgtcttc atggtggtat tccagtcaat tgtaggctgc atcattgacg
     2041 ccttcatcat tggtgcagtc atggcgaaga tggcaaagcc aaagaagaga aatgagactc
     2101 ttgtcttcag tcacaatgct gtgattgcca tgagggatgg caaactctgc ttgatgtgga
     2161 gagtgggtaa ccttcgaaag agccaccttg tggaagctca tgtccgggca cagcttctca
     2221 aatctaggat cacttcagaa ggggagtata tccctttgga ccagatagac atcaatgttg
     2281 gttttgatag tggaattgac cgcatatttc tagtgtcccc catcactatc gttcacgaaa
     2341 tagatgaaga cagcccttta tatgacttga gtaagcagga cattgacaat gcagactttg
     2401 aaattgttgt catactggaa ggcatggtgg aggcgactgc catgacaact caatgccgga
     2461 gttcgtatct ggccaatgaa attctctggg gtcaccgcta tgagccagtg ctctttgaag
     2521 agaaacacta ctataaagta gactattcaa gattccataa gacttatgaa gtacctaaca
     2581 cccccctttg tagtgccaga gacttagcag agaagaaata catcctttca aatgcaaatt
     2641 cattttgcta tgaaaatgaa gttgccctaa caagcaaaga ggaagaggag gatagtgaga
     2701 acggagtccc agagagcaca agcacagact cacctcctgg catagatctc cacaaccagg
     2761 caagcgtacc tctagagccc aggcccttaa ggcgagaatc ggagatatga ccctctagac
     2821 gcgcgatcac tagcttaatt aagaattccg cccccccccc ccccccctct ccctcccccc
     2881 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     2941 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     3001 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     3061 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     3121 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     3181 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     3241 ttgtggaaag agtcaaatgg ctctcctaag cgtattcaac aaggggctga aggatgccca
     3301 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     3361 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     3421 aaaaacacga tgataatatg gccacaacca tggctgagcc ccgccaggag ttcgacgtga
     3481 tggaagatca tgctcagggg gactacaccc tgcaagacca ggagggtgac atggaccccg
     3541 gcctgaaaga gtctcccctg cagaccccgg ccgatgatgg atctgaggaa ccaggctctg
     3601 aaacctctga tgctaagagc actccgacgg cggaagatgc gacagcaccc ttagtggatg
     3661 agggagcccc cggtgagcag gcggccgctc aggcccccgc ggagatccca gaaggaaccg
     3721 cagctgaaga agcaggcatt ggcgacacgt ccaacctgga agaccaagct gccggacacg
     3781 tgacccaagc tcgcatggtc agtaaaggca aagatgggac tggacccgat gacaaaaaaa
     3841 ccaagggggc ggatggtaag cctggaacga agattgccac accccgggga gcagcccctc
     3901 caggccagaa aggccaggcc aacgccaccc ggattccagc aaaaaccact cccaccccga
     3961 agacctcgcc agcaaccatg caagtgcaga aaaaaccacc ccctgcaggg gcaaaatctg
     4021 agagaggtga atctgggaaa tccggggacc gcagcggcta cagcagcccc ggctccccag
     4081 gcactccggg cagccgctcc cgcacaccct ccctgccgac cccgcccacc cgggagccca
     4141 agaaggtggc ggtggtccgc actcccccca agtcgccgtc tgcagccaag agccgcctgc
     4201 aggccgctcc cgggcccatg ccagacctga agaacgtcaa gtccaaaatc ggctccacgg
     4261 aaaacctgaa gcaccagcca ggaggtggca aggtgcagat aattaataag aagctggatc
     4321 ttagcaacgt ccagtccaag tgtggctcaa aggataatat caaacacgtg ccaggaggcg
     4381 gcagtgtgca aatagtctac aaaccagtgg atctgagcaa ggtgacctcc aagtgtggct
     4441 cattaggcaa catccatcat aagccaggag gtggccaagt ggaagtgaaa tctgagaagc
     4501 tggacttcaa ggatagagtc cagtcgaaga ttgggtccct ggacaacatc acacacgtcc
     4561 ctggcggagg gaataaaaag atcgaaaccc acaagctgac cttccgcgag aacgccaaag
     4621 ccaagaccga ccacggggcg gagatcgtgt ggatccccgt cgttttacaa cgtcgtgact
     4681 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     4741 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     4801 gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt
     4861 gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg cacggttacg
     4921 atgcgcccat ctacaccaac gtaacctatc ccattacggt caatccgccg tttgttccca
     4981 cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc tggctacagg
     5041 aaggccagac gcgaattatt tttgatggcg ttaactcggc gtttcatctg tggtgcaacg
     5101 ggcgctgggt cggttacggc caggacagtc gtttgccgtc tgaatttgac ctgagcgcat
     5161 ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct gcgttggagt gacggcagtt
     5221 atctggaaga tcaggatatg tggcggatga gcggcatttt ccgtgacgtc tcgttgctgc
     5281 ataaaccgac tacacaaatc agcgatttcc atgttgccac tcgctttaat gatgatttca
     5341 gccgcgctgt actggaggct gaagttcaga tgtgcggcga gttgcgtgac tacctacggg
     5401 taacagtttc tttatggcag ggtgaaacgc aggtcgccag cggcaccgcg cctttcggcg
     5461 gtgaaattat cgatgagcgt ggtggttatg ccgatcgcgt cacactacgt ctgaacgtcg
     5521 aaaacccgaa actgtggagc gccgaaatcc cgaatctcta tcgtgcggtg gttgaactgc
     5581 acaccgccga cggcacgctg attgaagcag aagcctgcga tgtcggtttc cgcgaggtgc
     5641 ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt gctgattcga ggcgttaacc
     5701 gtcacgagca tcatcctctg catggtcagg tcatggatga gcagacgatg gtgcaggata
     5761 tcctgctgat gaagcagaac aactttaacg ccgtgcgctg ttcgcattat ccgaaccatc
     5821 cgctgtggta cacgctgtgc gaccgctacg gcctgtatgt ggtggatgaa gccaatattg
     5881 aaacccacgg catggtgcca atgaatcgtc tgaccgatga tccgcgctgg ctaccggcga
     5941 tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt gtgatcatct
     6001 ggtcgctggg gaatgaatca ggccacggcg ctaatcacga cgcgctgtat cgctggatca
     6061 aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg cggagccgac accacggcca
     6121 ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc ccggctgtgc
     6181 cgaaatggtc catcaaaaaa tggctttcgc tacctggaga gacgcgcccg ctgatccttt
     6241 gcgaatacgc ccacgcgatg ggtaacagtc ttggcggttt cgctaaatac tggcaggcgt
     6301 ttcgtcagta tccccgttta cagggcggct tcgtctggga ctgggtggat cagtcgctga
     6361 ttaaatatga tgaaaacggc aacccgtggt cggcttacgg cggtgatttt ggcgatacgc
     6421 cgaacgatcg ccagttctgt atgaacggtc tggtctttgc cgaccgcacg ccgcatccag
     6481 cgctgacgga agcaaaacac cagcagcagt ttttccagtt ccgtttatcc gggcaaacca
     6541 tcgaagtgac cagcgaatac ctgttccgtc atagcgataa cgagctcctg cactggatgg
     6601 tggcgctgga tggtaagccg ctggcaagcg gtgaagtgcc tctggatgtc gctccacaag
     6661 gtaaacagtt gattgaactg cctgaactac cgcagccgga gagcgccggg caactctggc
     6721 tcacagtacg cgtagtgcaa ccgaacgcga ccgcatggtc agaagccggg cacatcagcg
     6781 cctggcagca gtggcgtctg gcggaaaacc tcagtgtgac gctccccgcc gcgtcccacg
     6841 ccatcccgca tctgaccacc agcgaaatgg atttttgcat cgagctgggt aataagcgtt
     6901 ggcaatttaa ccgccagtca ggctttcttt cacagatgtg gattggcgat aaaaaacaac
     6961 tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct ggataacgac attggcgtaa
     7021 gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg ctggaaggcg gcgggccatt
     7081 accaggccga agcagcgttg ttgcagtgca cggcagatac acttgctgat gcggtgctga
     7141 ttacgaccgc tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc cggaaaacct
     7201 accggattga tggtagtggt caaatggcga ttaccgttga tgttgaagtg gcgagcgata
     7261 caccgcatcc ggcgcggatt ggcctgaact gccagctggc gcaggtagca gagcgggtaa
     7321 actggctcgg attagggccg caagaaaact atcccgaccg ccttactgcc gcctgttttg
     7381 accgctggga tctgccattg tcagacatgt ataccccgta cgtcttcccg agcgaaaacg
     7441 gtctgcgctg cgggacgcgc gaattgaatt atggcccaca ccagtggcgc ggcgacttcc
     7501 agttcaacat cagccgctac agtcaacagc aactgatgga aaccagccat cgccatctgc
     7561 tgcacgcgga agaaggcaca tggctgaata tcgacggttt ccatatgggg attggtggcg
     7621 acgactcctg gagcccgtca gtatcggcgg aattccagct gagcgccggt cgctaccatt
     7681 accagttggt ctggtgtcaa aaataataat aaccgggcag ggggggatcc actagttcta
     7741 gagggcccta ttctatagtg tcacctaaat gctagaggat ctttgtgaag gaaccttact
     7801 tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata
     7861 taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga
     7921 ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc
     7981 tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcacattc
     8041 tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
     8101 aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
     8161 cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt tgatgtatag
     8221 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     8281 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     8341 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     8401 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     8461 atcatgtctg gatcgatccc gccatggtat caacgccata tttctattta cagtagggac
     8521 ctcttcgttg tgtaggtacc gctgtattcc tagggaaata gtagaggcac cttgaactgt
     8581 ctgcatcagc catatagccc ccgctgttcg atttacaaac acaggcacag tactgacaaa
     8641 cccatacacc tcctctgaaa tacccatagt tgctagggct gtctccgaac tcattacacc
     8701 ctccaaagtc agagctgtaa tttcgccatc aagggcagcg agggcttctc cagataaaat
     8761 agcttctgcc gagagtcccg taagggtaga cacttcagct aatccctcga gcgcggccgc
     8821 tctagagttt aaacggcgcg ccgtacccag cttttgtncc agcttttgtt ccctttagtg
     8881 agggttaatt tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     8941 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     9001 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9061 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     9121 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     9181 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     9241 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     9301 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     9361 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     9421 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     9481 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     9541 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9601 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9661 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9721 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     9781 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9841 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9901 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9961 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    10021 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    10081 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    10141 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    10201 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    10261 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    10321 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    10381 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    10441 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    10501 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    10561 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    10621 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    10681 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10741 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10801 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10861 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10921 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10981 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    11041 atttccccga aaagtgc
//
