LOCUS       sequence-380257-       12823 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral vector expressing sgRNA-5 targeting eIF3 binding site of 
            RPS15A 5'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12823)
  AUTHORS   Duan H, Zhang S, Zarai Y, Ollinger R, Wu Y, Sun L, Hu C, He Y, Tian 
            G, Rad R, Kong X, Cheng Y, Tuller T, Wolf DA
  TITLE     eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant 
            regulator of ribosome content.
  JOURNAL   EMBO J. 2023 Jun 15;42(12):e112362. doi: 10.15252/embj.2022112362. 
            Epub 2023 May 8.
  PUBMED    37155573
REFERENCE   2  (bases 1 to 12823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2023-06-15"; volume: "42(12):e112362. doi"; pages: " 
            10.15252/embj.2022112362. Epub 2023 May 8"
COMMENT     Sequence Label: RPS15A sgRNA-5 lentiCRISPR v2-Blast plasmid
FEATURES             Location/Qualifiers
     source          1..12823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..56
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        172..219
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             238..612
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    742..863
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(779..798)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     833..852
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(912..928)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(912..928)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(925..947)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    936..952
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(960..966,967..984,985..990))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1005..1026
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1143..1160)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1314..1902)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1394..1413)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2073..2864,2865..2933))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2696..2715
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2934..3038)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3113..3132)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3304..3683
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3685..3883
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3838..3858
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3901..4081
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4128..4253
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4746..4979
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5164..5208
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5506..5623
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5674..5922
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5674..5694
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5845..5864
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5943..6018
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        6080..6291
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      6310..6319
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6316..10419
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6316..10419
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10420..10467
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             10468..10491
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             10501..10557
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10558..10956
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    10981..11569
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11034..11054)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11452..11463)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11641..11874
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(11900..11917)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11906..12130
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12176..12604
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12263..12282)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12473..12494
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12613..12633)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(12618..12823,1..124)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(12798..12823,1..110)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
       61 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
      121 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
      181 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
      241 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
      301 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
      361 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
      421 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
      481 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
      541 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
      601 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
      661 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
      721 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
      781 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
      841 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
      901 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
      961 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     1021 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     1081 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     1141 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     1201 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     1261 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     1321 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1381 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1441 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1501 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1561 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1621 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1681 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1741 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1801 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1861 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1921 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1981 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     2041 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     2101 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     2161 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     2221 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     2281 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     2341 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     2401 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     2461 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     2521 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     2581 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     2641 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     2701 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     2761 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     2821 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     2881 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2941 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     3001 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     3061 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     3121 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     3181 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     3241 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     3301 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     3361 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     3421 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     3481 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     3541 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     3601 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     3661 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     3721 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     3781 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     3841 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact
     3901 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     3961 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     4021 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     4081 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     4141 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     4201 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     4261 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     4321 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     4381 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     4441 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     4501 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     4561 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     4621 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     4681 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     4741 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     4801 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     4861 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     4921 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     4981 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     5041 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     5101 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     5161 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     5221 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     5281 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     5341 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     5401 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     5461 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     5521 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     5581 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     5641 cagcagagat ccagtttggt taattaaggt accgagggcc tatttcccat gattccttca
     5701 tatttgcata tacgatacaa ggctgttaga gagataatta gaattaattt gactgtaaac
     5761 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
     5821 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
     5881 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgcatcctg caatctaagg
     5941 ttgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
     6001 gtggcaccga gtcggtgctt ttttgaattc gctagctagg tcttgaaagg agtgggaatt
     6061 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     6121 ggaggggtcg gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     6181 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     6241 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaccggttc
     6301 tagagcgctg ccaccatgga caagaagtac agcatcggcc tggacatcgg caccaactct
     6361 gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg
     6421 ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc
     6481 ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg
     6541 aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac
     6601 agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg
     6661 caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc
     6721 taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat
     6781 ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac
     6841 cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg
     6901 ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga
     6961 ctgagcaaga gcagacggct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat
     7021 ggcctgttcg gaaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac
     7081 ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg
     7141 gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac
     7201 ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc
     7261 cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg
     7321 aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc
     7381 aagaacggct acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc
     7441 atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaacaga
     7501 gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac
     7561 ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac
     7621 aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg
     7681 gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc
     7741 tggaacttcg aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg
     7801 accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac
     7861 gagtacttca ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga
     7921 aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc
     7981 aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc
     8041 gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac
     8101 gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt
     8161 ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg
     8221 ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga
     8281 tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc
     8341 ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag
     8401 ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc
     8461 cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag
     8521 ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag
     8581 cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag
     8641 aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc
     8701 ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac
     8761 ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac
     8821 tacgatgtgg accatatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag
     8881 gtgctgacca gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc
     8941 gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga
     9001 aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc
     9061 ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg
     9121 gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg
     9181 atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg
     9241 cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc
     9301 gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg
     9361 tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag
     9421 tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc
     9481 gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat
     9541 aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg
     9601 aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac
     9661 agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac
     9721 agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag
     9781 aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag
     9841 aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc
     9901 atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc
     9961 tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc
    10021 ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa
    10081 cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag
    10141 ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac
    10201 aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg
    10261 accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg
    10321 tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg
    10381 tacgagacac ggatcgacct gtctcagctg ggaggcgaca agcgacctgc cgccacaaag
    10441 aaggctggac aggctaagaa gaagaaagat tacaaagacg atgacgataa gggatccggc
    10501 gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg
    10561 gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac
    10621 agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc
    10681 atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg
    10741 ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat
    10801 gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat
    10861 cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt
    10921 gaattgctgc cctctggtta tgtgtgggag ggctaagaat tcgatatcaa gcttatcggt
    10981 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
    11041 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
    11101 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
    11161 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
    11221 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
    11281 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
    11341 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
    11401 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
    11461 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
    11521 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
    11581 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
    11641 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
    11701 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
    11761 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
    11821 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
    11881 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
    11941 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
    12001 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
    12061 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
    12121 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
    12181 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
    12241 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    12301 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
    12361 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
    12421 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    12481 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    12541 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    12601 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
    12661 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
    12721 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
    12781 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttc
//
