LOCUS       sequence-380258-        9201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression of SLC2A1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9201)
  AUTHORS   Kim M, Ly SH, Xie Y, Duronio GN, Ford-Roshon D, Hwang JH, Sulahian 
            R, Rennhack JP, So J, Gjoerup O, Talamas JA, Grandclaudon M, Long 
            HW, Doench JG, Sethi NS, Giannakis M, Hahn WC
  TITLE     YAP1 and PRDM14 converge to promote cell survival and tumorigenesis.
  JOURNAL   Dev Cell. 2022 Jan 24;57(2):212-227.e8. doi: 
            10.1016/j.devcel.2021.12.006. Epub 2022 Jan 5.
  PUBMED    34990589
REFERENCE   2  (bases 1 to 9201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.12.006"; journalName: "Dev Cell"; date: 
            "2022-01-24- 24"; volume: "57"; issue: "2"; pages: "212-227.e8"
COMMENT     Sequence Label: pLX304 SLC2A1
FEATURES             Location/Qualifiers
     source          1..9201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        95..398
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        399..602
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     553..573
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    625..649
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2129..2153)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2155..2196
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2238..2826
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2291..2311)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2709..2720)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2898..3131
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3209..3330
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3246..3265)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3300..3319
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3370..3505
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3525..3544)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3526..3544)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3554..3571)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3554..3570)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3563..3585)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3712..4167
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3799..3818)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4009..4030
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4193..4297
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4298..4366,4367..5158)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4516..4535)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5329..5917
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5818..5837
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6071..6088
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6205..6226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6241..6246,6247..6264,6265..6271)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6279..6295
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6284..6306
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6303..6319
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6303..6319
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6338..6358
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6340..6358
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6386..6612
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6613..6793
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6840..6965
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7458..7691
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7876..7920
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8095..8595
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8514..8532
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8607..9002
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9062..9179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gagatccact ttggcgccgg ctcgaggggg cccggggtta ttaatagtaa tcaattacgg
       61 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      121 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      421 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      481 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      541 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      601 ctctctggct aactgtcggg atcaacaagt ttgtacaaaa aagttggcac catggagccc
      661 agcagcaaga agctgacggg tcgcctcatg ctggccgtgg gaggagcagt gcttggctcc
      721 ctgcagtttg gctacaacac tggagtcatc aatgcccccc agaaggtgat cgaggagttc
      781 tacaaccaga catgggtcca ccgctatggg gagagcatcc tgcccaccac gctcaccacg
      841 ctctggtccc tctcagtggc catcttttct gttgggggca tgattggctc cttctctgtg
      901 ggccttttcg ttaaccgctt tggccggcgg aattcaatgc tgatgatgaa cctgctggcc
      961 ttcgtgtccg ccgtgctcat gggcttctcg aaactgggca agtcctttga gatgctgatc
     1021 ctgggccgct tcatcatcgg tgtgtactgc ggcctgacca caggcttcgt gcccatgtat
     1081 gtgggtgaag tgtcacccac agcccttcgt ggggccctgg gcaccctgca ccagctgggc
     1141 atcgtcgtcg gcatcctcat cgcccaggtg ttcggcctgg actccatcat gggcaacaag
     1201 gacctgtggc ccctgctgct gagcatcatc ttcatcccgg ccctgctgca gtgcatcgtg
     1261 ctgcccttct gccccgagag tccccgcttc ctgctcatca accgcaacga ggagaaccgg
     1321 gccaagagtg tgctaaagaa gctgcgcggg acagctgacg tgacccatga cctgcaggag
     1381 atgaaggaag agagtcggca gatgatgcgg gagaagaagg tcaccatcct ggagctgttc
     1441 cgctcccccg cctaccgcca gcccatcctc atcgctgtgg tgctgcagct gtcccagcag
     1501 ctgtctggca tcaacgctgt cttctattac tccacgagca tcttcgagaa ggcgggggtg
     1561 cagcagcctg tgtatgccac cattggctcc ggtatcgtca acacggcctt cactgtcgtg
     1621 tcgctgtttg tggtggagcg agcaggccgg cggaccctgc acctcatagg cctcgctggc
     1681 atggcgggtt gtgccatact catgaccatc gcgctagcac tgctggagca gctaccctgg
     1741 atgtcctatc tgagcatcgt ggccatcttt ggctttgtgg ccttctttga agtgggtcct
     1801 ggccccatcc catggttcat cgtggctgaa ctcttcagcc agggtccacg tccagctgcc
     1861 attgccgttg caggcttctc caactggacc tcaaatttca ttgtgggcat gtgcttccag
     1921 tatgtggagc aactgtgtgg tccctacgtc ttcatcatct tcactgtgct cctggttctg
     1981 ttcttcatct tcacctactt caaagttcct gagactaaag gccggacctt cgatgagatc
     2041 gcttccggct tccggcaggg gggagccagc caaagtgaca agacacccga ggagctgttc
     2101 catcccctgg gggctgattc ccaagtgttg ccaactttct tgtacaaagt ggttggtaag
     2161 cctatcccta accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg
     2221 tggcgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     2281 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     2341 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     2401 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     2461 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     2521 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     2581 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     2641 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     2701 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     2761 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     2821 ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta gccacttttt
     2881 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     2941 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     3001 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     3061 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     3121 aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc
     3181 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     3241 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     3301 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     3361 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3421 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     3481 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     3541 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     3601 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     3661 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     3721 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3781 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3841 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     3901 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     3961 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4021 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     4081 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4141 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     4201 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4261 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     4321 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     4381 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     4441 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     4501 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     4561 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     4621 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4681 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4741 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4801 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     4861 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     4921 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     4981 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     5041 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     5101 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     5161 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     5221 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     5281 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5341 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5401 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5461 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5521 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5581 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5641 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5701 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5761 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5821 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5881 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5941 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     6001 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     6061 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     6121 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     6181 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     6241 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     6301 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     6361 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
     6421 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
     6481 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
     6541 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
     6601 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     6661 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     6721 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     6781 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     6841 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     6901 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     6961 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     7021 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     7081 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     7141 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     7201 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     7261 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     7321 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     7381 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     7441 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     7501 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     7561 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     7621 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     7681 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     7741 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     7801 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     7861 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     7921 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     7981 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     8041 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccttgcgc
     8101 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
     8161 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
     8221 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
     8281 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
     8341 actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg
     8401 gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg
     8461 gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc
     8521 gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc
     8581 gacctctctc cccagggggt accaccatgg ccaagccttt gtctcaagaa gaatccaccc
     8641 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg
     8701 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta
     8761 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca
     8821 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac
     8881 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg
     8941 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg
     9001 gcctgcagct gcagtagtaa gaattctaga tcttgagaca aatggcagta ttcatccaca
     9061 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     9121 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     9181 gggtttatta cagggacagc a
//
