LOCUS       sequence-380309-       10849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression of Luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10849)
  AUTHORS   Kim M, Ly SH, Xie Y, Duronio GN, Ford-Roshon D, Hwang JH, Sulahian 
            R, Rennhack JP, So J, Gjoerup O, Talamas JA, Grandclaudon M, Long 
            HW, Doench JG, Sethi NS, Giannakis M, Hahn WC
  TITLE     YAP1 and PRDM14 converge to promote cell survival and tumorigenesis.
  JOURNAL   Dev Cell. 2022 Jan 24;57(2):212-227.e8. doi: 
            10.1016/j.devcel.2021.12.006. Epub 2022 Jan 5.
  PUBMED    34990589
REFERENCE   2  (bases 1 to 10849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.12.006"; journalName: "Dev Cell"; date: 
            "2022-01-24- 24"; volume: "57"; issue: "2"; pages: "212-227.e8"
COMMENT     Sequence Label: pLX307 P2A GFP Luciferase
FEATURES             Location/Qualifiers
     source          1..10849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     102..121
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(153..349)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(161..371)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      167..302
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(602..646)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    831..1064
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1557..1682
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1729..1909
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1910..2136)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2164..2184)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2164..2182)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2203..2219)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2203..2219)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2216..2238)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2227..2243
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2251..2257,2258..2275,2276..2281))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2296..2317
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2434..2451)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2605..3193)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2685..2704)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3364..4155,4156..4224))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3987..4006
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4225..4329)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4355..4810)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4492..4513)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4704..4723
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4937..4959
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4951..4968
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4952..4968
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4978..4997
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4978..4996
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      5017..5152
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5192..5313
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5203..5222)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5257..5276
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             5391..5624
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5696..6284
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5802..5813
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6211..6231
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(6335..7048,7049..7051,7052..7054))
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6380..6401)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6729..6748
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6988..7009
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(7055..7111)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(7121..7162)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    7170..7194
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(7196..8845)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     8761..8779
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     protein_bind    complement(8848..8872)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(8892..10070)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8901..9839
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(8924..8944)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    10208..10325
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(join(10368..10849,1..121))
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10454..10474)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 ggtccggatc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg gcgagggtgc
       61 gtacggccct ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg tactcggtca
      121 tggtaagctc cggtggtacc tctagaaagc tttttgcaaa agcctaggcc tccaaaaaag
      181 cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata
      241 aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg
      301 atgggcggag ttaggggcgg gactatggtt gctgactaat tgagacgcat gctttgcata
      361 cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta attgagatgc
      421 aatgcatggg tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct
      481 gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct accaagcctc
      541 ctactatcat tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc
      601 acaaacttgc ccatttatct aattccaata attcttgttc attcttttct tgctggtttt
      661 gcgattcttc aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact
      721 ccatccaggt cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc
      781 aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt
      841 gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga
      901 ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct
      961 gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca
     1021 tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt
     1081 tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt
     1141 ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag
     1201 gtctgaagat cagcggccgc ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct
     1261 atcttgtcta aagcttcctt ggtgtctttt atctctatcc tttgatgcac acaatagagg
     1321 gttgctactg tattatataa tgatctaagt tcttctgatc ctgtctgaag ggatggttgt
     1381 agctgtccca gtatttgtct acagccttct gatgtttcta acaggccagg attaactgcg
     1441 aatcgttcta gctccctgct tgcccatact atatgtttta atttatattt tttctttccc
     1501 cctggcctta accgaatttt ttcccatcgc gatctaattc tcccccgctt aatactgacg
     1561 ctctcgcacc catctctctc cttctagcct ccgctagtca aaatttttgg cgtactcacc
     1621 agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag
     1681 agctcctctg gtttcccttt cgctttcaag tccctgttcg ggcgccactg ctagagattt
     1741 tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg
     1801 cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta
     1861 gttagccaga gagctcccag gctcagatct ggtctaacca gagagacccg tttatgtatc
     1921 gagctaggca cttaaataca atatctctgc aatgcggcaa ttcagtggtt cgtccaatcc
     1981 atgtcagacc cgtctgttgc cttcctaata aggcacgatc gtaccacctt acttccacca
     2041 atcggcatgc acggtgcttt ttctctcctt gtaaggcatg ttgctaactc atcgttacca
     2101 tgttgcaaga ctacaagagt attgcataag actacattaa gcttgcagct ccagcttttg
     2161 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     2221 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     2281 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     2341 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     2401 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2461 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2521 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2581 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2641 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2701 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2761 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     2821 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2881 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2941 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3001 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     3061 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3121 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3181 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3241 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3301 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3361 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3421 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     3481 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     3541 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     3601 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     3661 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     3721 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     3781 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     3841 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     3901 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     3961 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     4021 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4081 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4141 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     4201 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     4261 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     4321 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
     4381 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     4441 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     4501 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     4561 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     4621 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     4681 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     4741 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     4801 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     4861 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     4921 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa
     4981 tacgactcac tatagggcga attgggtacg tccctaggcc tccaaaaaag cctcctcact
     5041 acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta
     5101 gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag
     5161 ttaggggcgg gatagctaga gccagacatg ataagataca ttgatgagtt tggacaaacc
     5221 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     5281 tttgtaacca ttataagctg caataaacaa gttcctctca ctctctgata ttcatttctt
     5341 tgcaagttat aaatactgaa taataagatg acatgaacta ctatacgtac tgctagagat
     5401 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
     5461 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
     5521 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaagc
     5581 aaaaagcaga tcttgtcttc gttgggagtg aattagccct tccagtcccc ccttttcttt
     5641 taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag tcgacgcggg
     5701 gaggcggccc aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc
     5761 cgcagagccg gcagcaggcc gcgggaagga aggtccgctg gattgagggc cgaagggacg
     5821 tagcagaagg acgtcccgcg cagaatccag gtggcaacac aggcgagcag ccaaggaaag
     5881 gacgatgatt tccccgacaa caccacggaa ttgtcagtgc ccaacagccg agcccctgtc
     5941 cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg caatagggag ggggaaagcg
     6001 aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca
     6061 gcaaacacag tgcacaccac gccacgttgc ctgacaacgg gccacaactc ctcataaaga
     6121 gacagcaacc aggatttata caaggaggag aaaatgaaag ccatacggga agcaatagca
     6181 tgatacaaag gcattaaagc agcgtatcca catagcgtaa aaggagcaac atagttaaga
     6241 ataccagtca atctttcaca aattttgtaa tccagaggtt gattgtcgac ttaacgcgtt
     6301 gattgtcgac ttaaccggta ctagttcatt actattactt gtacagctcg tccatgccga
     6361 gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg
     6421 ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc
     6481 cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga
     6541 tgttgtggcg ggtcttgaag ttcaccttga tgccgttctt ctgcttgtcg gcggtgatat
     6601 agaccttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct
     6661 tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct
     6721 cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc
     6781 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga
     6841 agcactgcac gccgtaggtc aaggtggtca cgagggtggg ccagggcacg ggcagcttgc
     6901 cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
     6961 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
     7021 ccccggtgaa cagctcctcg cccttgctca ccataggccc ggggttttct tcaacatcac
     7081 ctgcttgctt tagcagagag aagtttgtgg cgccgctgcc cgtagaatcg agaccgagga
     7141 gagggttagg gataggctta ccgatatcaa ccactttgta caagaaagtt ggcaacacgg
     7201 cgatctttcc gcccttcttg gcctttatga ggatctctct gatttttctt gcgtcgagtt
     7261 ttccggtaag acctttcggt acttcgtcca caaacacaac tcctccgcgc aactttttcg
     7321 cggttgttac ttgactggcg acgtaatcca cgatctcttt ttccgtcatc gtctttccgt
     7381 gctccaaaac aacaacggcg gcgggaagtt caccggcgtc atcgtcggga agacctgcga
     7441 cacctgcgtc gaagatgttg gggtgttgga gcaagatgga ttccaattca gcgggagcca
     7501 cctgatagcc tttgtactta atcagagact tcaggcggtc aacgatgaag aagtgttcgt
     7561 cttcgtccca gtaagctatg tctccagaat gtagccatcc atccttgtca atcaaggcgt
     7621 tggtcgcttc cggattgttt acataaccgg acataatcat aggacctctc acacacagtt
     7681 cgcctctttg attaacgccc agcgttttcc cggtatccag atccacaacc ttcgcttcaa
     7741 aaaatggaac aactttaccg accgcgcccg gtttatcatc cccctcgggt gtaatcagaa
     7801 tagctgatgt agtctcagtg agcccatatc cttgcctgat acctggcaga tggaacctct
     7861 tggcaaccgc ttccccgact tccttagaga ggggagcgcc accagaagca atttcgtgta
     7921 aattagataa atcgtatttg tcaatcagag tgcttttggc gaagaaggag aatagggttg
     7981 gcaccagcag cgcactttga atcttgtaat cctgaaggct cctcagaaac agctcttctt
     8041 caaatctata cattaagacg actcgaaatc cacatatcaa atatccgagt gtagtaaaca
     8101 ttccaaaacc gtgatggaat ggaacaacac ttaaaatcgc agtatccgga atgatttgat
     8161 tgccaaaaat aggatctctg gcatgcgaga atctcacgca ggcagttcta tgaggcagag
     8221 cgacaccttt aggcagacca gtagatccag aggagttcat gatcagtgca attgtcttgt
     8281 ccctatcgaa ggactctggc acaaaatcgt attcattaaa accgggaggt agatgagatg
     8341 tgacgaacgt gtacatcgac tgaaatccct ggtaatccgt tttagaatcc atgataataa
     8401 ttttttggat gattgggagc tttttttgca cgttcaaaat tttttgcaac ccctttttgg
     8461 aaacgaacac cacggtaggc tgcgaaatgc ccatactgtt gagcaattca cgttcattat
     8521 aaatgtcgtt cgcgggcgca actgcaactc cgataaataa cgcgcccaac accggcataa
     8581 agaattgaag agagttttca ctgcatacga cgattctgtg atttgtattc agcccatatc
     8641 gtttcatagc ttctgccaac cgaacggaca tttcgaagta ctcagcgtaa gtgatgtcca
     8701 cctcgatatg tgcatctgta aaagcaattg ttccaggaac cagggcgtat ctcttcatag
     8761 ccttatgcag ttgctctcca gcggttccat cttccagcgg atagaatggc gccgggcctt
     8821 tctttatgtt tttggcgtct tccatggtgc caactttttt gtacaaactt gttgatcaat
     8881 cgatgctagc ctcacgacac ctgaaatgga agaaaaaaac tttgaaccac tgtctgaggc
     8941 ttgagaatga accaagatcc aaactcaaaa agggcaaatt ccaaggagaa ttacatcaag
     9001 tgccaagctg gcctaacttc agtctccacc cactcagtgt ggggaaactc catcgcataa
     9061 aacccctccc cccaacctaa agacgacgta ctccaaaagc tcgagaacta atcgaggtgc
     9121 ctggacggcg cccggtactc cgtggagtca catgaagcga cggctgagga cggaaaggcc
     9181 cttttccttt gtgtgggtga ctcacccgcc cgctctcccg agcgccgcgt cctccatttt
     9241 gagctccctg cagcagggcc gggaagcggc catctttccg ctcacgcaac tggtgccgac
     9301 cgggccagcc ttgccgccca gggcggggcg atacacggcg gcgcgaggcc aggcaccaga
     9361 gcaggccggc cagcttgaga ctacccccgt ccgattctcg gtggccgcgc tcgcaggccc
     9421 cgcctcgccg aacatgtgcg ctgggacgca cgggccccgt cgccgcccgc ggccccaaaa
     9481 accgaaatac cagtgtgcag atcttggccc gcatttacaa gactatcttg ccagaaaaaa
     9541 agcgtcgcag caggtcatca aaaattttaa atggctagag acttatcgaa agcagcgaga
     9601 caggcgcgaa ggtgccacca gattcgcacg cggcggcccc agcgcccagg ccaggcctca
     9661 actcaagcac gaggcgaagg ggctccttaa gcgcaaggcc tcgaactctc ccacccactt
     9721 ccaacccgaa gctcgggatc aagaatcacg tactgcagcc aggtggaagt aattcaaggc
     9781 acgcaagggc cataacccgt aaagaggcca ggcccgcggg aaccacacac ggcacttacc
     9841 tgtgttctgg cggcaaaccc gttgcgaaaa agaacgttca cggcgactac tgcacttata
     9901 tacggttctc ccccaccctc gggaaaaagg cggagccagt acacgacatc actttcccag
     9961 tttaccccgc gccaccttct ctaggcaccg gttcaattgc cgacccctcc ccccaacttc
    10021 tcggggactg tgggcgatgt gcgctctgcc cactgacggg caccggagcc aattcccact
    10081 cctttcaaga cctagaaggt ccattagctg caaagattcc tctctgttta aaactttatc
    10141 catctttgca cctgcaggcc tcgagccggc gccaaagtgg atctctgctg tccctgtaat
    10201 aaacccgaaa attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg
    10261 ttgctattat gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt
    10321 taaaattgtg gatgaatact gccatttgtc tcaagatcta gaattcgtta actggcaccg
    10381 ggcttgcggg tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg
    10441 gtgaagccga gccgctcgta gaaggggagg ttgcggggcg cggaggtctc caggaaggcg
    10501 ggcaccccgg cgcgctcggc cgcctccact ccggggagca cgacggcgct gcccagaccc
    10561 ttgccctggt ggtcgggcga gacgccgacg gtggccagga accacgcggg ctccttgggc
    10621 cggtgcggcg ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga accgctcaac
    10681 tcggccatgc gcgggccgat ctcggcgaac accgcccccg cttcgacgct ctccggcgtg
    10741 gtccagaccg ccacggcggc gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg
    10801 acgcgcgtga ggaagagttc ttgcagctcg gtgacccgct cgatgtggc
//
