LOCUS       sequence-380312-       10714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression of YAP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10714)
  AUTHORS   Kim M, Ly SH, Xie Y, Duronio GN, Ford-Roshon D, Hwang JH, Sulahian 
            R, Rennhack JP, So J, Gjoerup O, Talamas JA, Grandclaudon M, Long 
            HW, Doench JG, Sethi NS, Giannakis M, Hahn WC
  TITLE     YAP1 and PRDM14 converge to promote cell survival and tumorigenesis.
  JOURNAL   Dev Cell. 2022 Jan 24;57(2):212-227.e8. doi: 
            10.1016/j.devcel.2021.12.006. Epub 2022 Jan 5.
  PUBMED    34990589
REFERENCE   2  (bases 1 to 10714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.12.006"; journalName: "Dev Cell"; date: 
            "2022-01-24- 24"; volume: "57"; issue: "2"; pages: "212-227.e8"
COMMENT     Sequence Label: pLX307 P2A GFP YAP1
FEATURES             Location/Qualifiers
     source          1..10714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     133..152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     386..403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    520..541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(556..561,562..579,580..586)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    594..610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     599..621
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     618..634
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     618..634
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     653..673
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        655..673
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        701..927
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             928..1108
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1155..1280
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1773..2006
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2191..2235
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2466..2676
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2488..2684
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2535..2670
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2716..3318
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2716..2735)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3212..3232
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3361..3478
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3616..4794
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3847..4785
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4742..4762
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    4814..4838
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4835..4844
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(6357..6381)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6389..6430
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6440..6496
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(6497..6499,6500..6502,6503..7216)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6542..6563)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6803..6822)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7150..7171
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7267..7855
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7320..7340)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7738..7749)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7927..8160
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8238..8359
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8275..8294)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8329..8348
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8399..8534
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8554..8573)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8555..8573)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8583..8600)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8583..8599)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8592..8614)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8741..9196
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8828..8847)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9038..9059
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9222..9326
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9327..9395,9396..10187)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9545..9564)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10358..10714,1..232)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
       61 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      481 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
      541 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
      601 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
      661 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
      721 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
      781 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
      841 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
      901 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
      961 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     1021 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     1081 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     1141 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     1201 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     1261 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     1321 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     1381 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     1441 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     1501 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     1561 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     1621 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     1681 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     1741 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     1801 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     1861 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     1921 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     1981 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     2041 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     2101 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     2161 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     2221 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     2281 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     2341 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     2401 aggggaccca tgcattgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     2461 ccccagcagg cagaagtatg caaagcatgc gtctcaatta gtcagcaacc atagtcccgc
     2521 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     2581 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     2641 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt tctagaggta
     2701 ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     2761 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     2821 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     2881 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc
     2941 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     3001 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     3061 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     3121 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     3181 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     3241 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     3301 aagcccggtg ccagttaacg aattctagat cttgagacaa atggcagtat tcatccacaa
     3361 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     3421 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     3481 ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggcct gcaggtgcaa
     3541 agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg
     3601 aaaggagtgg gaattggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     3661 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
     3721 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     3781 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     3841 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
     3901 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
     3961 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
     4021 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
     4081 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
     4141 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
     4201 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     4261 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     4321 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     4381 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     4441 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     4501 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     4561 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     4621 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     4681 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     4741 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaggctag
     4801 catcgattga tcaacaagtt tgtacaaaaa agttgccacc atggatcccg ggcagcagcc
     4861 gccgcctcaa ccggcccccc agggccaagg gcagccgcct tcgcagcccc cgcaggggca
     4921 gggcccgccg tccggacccg ggcaaccggc acccgcggcg acccaggcgg cgccgcaggc
     4981 accccccgcc gggcatcaga tcgtgcacgt ccgcggggac tcggagaccg acctggaggc
     5041 gctcttcaac gccgtcatga accccaagac ggccaacgtg ccccagaccg tgcccatgag
     5101 gctccggaag ctgcccgact ccttcttcaa gccgccggag cccaaatccc actcccgaca
     5161 ggccagtact gatgcaggca ctgcaggagc cctgactcca cagcatgttc gagctcattc
     5221 ctctccagct tctctgcagt tgggagctgt ttctcctggg acactgaccc ccactggagt
     5281 agtctctggc ccagcagcta cacccacagc tcagcatctt cgacagtctt cttttgagat
     5341 acctgatgat gtacctctgc cagcaggttg ggagatggca aagacatctt ctggtcagag
     5401 atacttctta aatcacatcg atcagacaac aacatggcag gaccccagga aggccatgct
     5461 gtcccagatg aacgtcacag cccccaccag tccaccagtg cagcagaata tgatgaactc
     5521 ggcttcaggt cctcttcctg atggatggga acaagccatg actcaggatg gagaaattta
     5581 ctatataaac cataagaaca agaccacctc ttggctagac ccaaggcttg accctcgttt
     5641 tgccatgaac cagagaatca gtcagagtgc tccagtgaaa cagccaccac ccctggctcc
     5701 ccagagccca cagggaggcg tcatgggtgg cagcaactcc aaccagcagc aacagatgcg
     5761 actgcagcaa ctgcagatgg agaaggagag gctgcggctg aaacagcaag aactgcttcg
     5821 gcaggcaatg cggaatatcg atcccagcac agcaaattct ccaaaatgtc aggagttagc
     5881 cctgcgtagc cagttaccaa cactggagca ggatggtggg actcaaaatc cagtgtcttc
     5941 tcccgggatg tctcaggaat tgagaacaat gacgaccaat agctcagatc ctttccttaa
     6001 cagtggcacc tatcactctc gagatgagag tacagacagt ggactaagca tgagcagcta
     6061 cagtgtccct cgaaccccag atgacttcct gaacagtgtg gatgagatgg atacaggtga
     6121 tactatcaac caaagcaccc tgccctcaca gcagaaccgt ttcccagact accttgaagc
     6181 cattcctggg acaaatgtgg accttggaac actggaagga gatggaatga acatagaagg
     6241 agaggagctg atgccaagtc tgcaggaagc tttgagttct gacatcctta atgacatgga
     6301 gtctgttttg gctgccacca agctagataa agaaagcttt cttacatggt tataataccc
     6361 aactttcttg tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct
     6421 cgattctacg ggcagcggcg ccacaaactt ctctctgcta aagcaagcag gtgatgttga
     6481 agaaaacccc gggcctatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     6541 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     6601 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     6661 cgtgccctgg cccaccctcg tgaccacctt gacctacggc gtgcagtgct tcgcccgcta
     6721 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     6781 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     6841 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     6901 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaaggtct atatcaccgc
     6961 cgacaagcag aagaacggca tcaaggtgaa cttcaagacc cgccacaaca tcgaggacgg
     7021 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     7081 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     7141 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     7201 cgagctgtac aagtaatagt aatgaactag taccggttaa gtcgacaatc aacgcgttaa
     7261 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     7321 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     7381 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     7441 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     7501 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     7561 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7621 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     7681 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7741 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7801 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     7861 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     7921 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     7981 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     8041 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     8101 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     8161 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     8221 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     8281 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     8341 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     8401 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8461 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     8521 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     8581 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     8641 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     8701 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     8761 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     8821 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     8881 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     8941 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     9001 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     9061 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     9121 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     9181 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     9241 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     9301 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     9361 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     9421 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     9481 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     9541 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     9601 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     9661 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     9721 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     9781 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     9841 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     9901 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     9961 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    10021 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    10081 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    10141 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    10201 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    10261 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    10321 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    10381 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    10441 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    10501 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    10561 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    10621 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    10681 ggggttcgtg cacacagccc agcttggagc gaac
//
