LOCUS       sequence-38035-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Wei JR, Krishnamoorthy V, Murphy K, Kim JH, Schnappinger D, Alber T,
            Sassetti CM, Rhee KY, Rubin EJ
  TITLE     Depletion of antibiotic targets has widely varying effects on 
            growth.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 8;108(10):4176-81. Epub 2011 Feb 
            22.
  PUBMED    21368134
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2011-03-8"; volume: "108(10)"; pages: 
            "4176-81. Epub 2011 Feb 22"
COMMENT     Sequence Label: pJW1
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      1..357
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(415..438)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gtacgtacta agctctcatg tttcacgtac taagctctca tgtttaacgt actaagctct
       61 catgtttaac gaactaaacc ctcatggcta acgtactaag ctctcatggc taacgtacta
      121 agctctcatg tttcacgtac taagctctca tgtttgaaca ataaaattaa tataaatcag
      181 caacttaaat agcctctaag gttttaagtt ttataagaaa aaaaagaata tataaggctt
      241 ttaaagcttt taaggtttaa cggttgtgga caacaagcca gggatgtaac gcactgagaa
      301 gcccttagag cctctcaaag caattttgag tgacacagga acacttaacg gctgacatgg
      361 ctgcagctac gaggcgtcgg cgtagttctc cgagtagttc tcgtcgttgg cggccttgtc
      421 gtcgtcgtcc ttgtagtctc tagattgaat tctgattaga aaaactcatc gagcatcaaa
      481 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
      541 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
      601 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
      661 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt
      721 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
      781 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga
      841 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcg
//
