LOCUS       sequence-380661-       11019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generating lentiviruses to express Dlx2 and hygromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11019)
  AUTHORS   Yang N, Chanda S, Marro S, Ng YH, Janas JA, Haag D, Ang CE, Tang Y, 
            Flores Q, Mall M, Wapinski O, Li M, Ahlenius H, Rubenstein JL, Chang
            HY, Buylla AA, Sudhof TC, Wernig M
  TITLE     Generation of pure GABAergic neurons by transcription factor 
            programming.
  JOURNAL   Nat Methods. 2017 May 15. doi: 10.1038/nmeth.4291.
  PUBMED    28504679
REFERENCE   2  (bases 1 to 11019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 May 15. doi: 10.1038/nmeth.4291."
COMMENT     Sequence Label: DLX2-hygro
FEATURES             Location/Qualifiers
     source          1..11019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        151..530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        532..730
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             748..928
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    975..1100
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1593..1826
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2011..2055
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2353..2470
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2527..2545,2546..2568,2569..2587,2588..2610,2611..2629,
                     2630..2652,2653..2671,2672..2694,2695..2713,2714..2736,
                     2737..2755,2756..2778,2779..2797)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2830..2868
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2865..2889
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3904..3930
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     misc_feature    3976..4543
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3993..4545
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4137..4154)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4364..4383
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4560..5585
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4560..5585
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    5624..6212
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5677..5697)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6095..6106)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6215..6231)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6216..6232)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6737..6917
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6943..6960)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6949..7173
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7219..7647
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7306..7325)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7516..7537
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7656..7676)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7661..7990
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7841..7976
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7903..7922
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8038..8085
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8104..8478
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8608..8729
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8645..8664)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8699..8718
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8778..8794)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8778..8794)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8791..8813)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8802..8818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8826..8832,8833..8850,8851..8856))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8871..8892
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9009..9026)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9180..9768)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9260..9279)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9939..10730,10731..10799))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10562..10581
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10800..10904)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10979..10998)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
       61 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
      121 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
      181 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      241 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      301 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      361 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      421 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      481 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      541 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      601 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      661 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      721 atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
      781 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
      841 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
      901 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
      961 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     1021 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     1081 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     1141 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     1201 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     1261 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     1321 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     1381 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     1441 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     1501 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     1561 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     1621 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     1681 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     1741 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     1801 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     1861 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     1921 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     1981 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     2041 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     2101 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     2161 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     2221 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     2281 tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt
     2341 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     2401 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     2461 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag atctcgagtt
     2521 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2581 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2641 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2701 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     2761 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agctcggtac
     2821 ccgggtcgag gtaggcgtgt acggtgggag gcctatataa gcagagctcg tttagtgaac
     2881 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac
     2941 cgatccagcc tccgcggccc cgaattcgcc accatgactg gagtctttga cagtctggtg
     3001 gctgatatgc actcgaccca gatcaccgcc tccagcacgt accaccagca ccagcagccc
     3061 ccgagcggtg cgggcgccgg ccctggcggc aacagcaaca gcagcagcag caacagcagc
     3121 ctgcacaagc cccaggagtc gccaaccctc ccggtgtcca cggctacgga cagcagctac
     3181 tacaccaacc agcagcaccc ggcgggcggc ggcggcgggg gggcctcgcc ctacgcgcac
     3241 atgggctcct accagtacca cgccagcggc ctcaacaatg tctcctactc cgccaaaagc
     3301 agctacgacc tgggctacac cgccgcgtac acctcctacg cgccctacgg caccagttcg
     3361 tctccggtca acaacgagcc ggacaaggaa gaccttgagc ctgaaatccg aatagtgaac
     3421 gggaagccaa agaaagtccg gaaaccacgc accatctact ccagtttcca gctggcggcc
     3481 cttcaacgac gcttccagaa gacccagtat ctggccctgc cagagcgagc cgagctggcg
     3541 gcgtccctgg gcctcaccca aactcaggtc aaaatctggt tccagaaccg ccgatccaag
     3601 ttcaagaaga tgtggaaaag cggcgagata cccaccgagc agcaccctgg agccagcgct
     3661 tctcctcctt gtgcctcccc gccggtctcg gcgccagcat cctgggactt cggcgcgccg
     3721 cagcggatgg ctggcggcgg cccgggcagc ggaggcggcg gtgcgggcag ctctggctcc
     3781 agcccgagca gcgccgcctc ggcctttctg ggaaactacc cgtggtacca ccaggcttcg
     3841 ggctccgctt cacacctgca ggccacagcg ccacttctgc atccttcgca gactccgcag
     3901 gcgcaccatc accaccatca ccaccaccac gcaggcgggg gcgccccggt gagcgcgggg
     3961 acgattttct aaggatccct cccccccccc taacgttact ggccgaagcc gcttggaata
     4021 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     4081 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     4141 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     4201 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     4261 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     4321 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     4381 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     4441 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     4501 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acagctagaa
     4561 tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca
     4621 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg
     4681 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc
     4741 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg
     4801 gggaatttag cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc
     4861 aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg
     4921 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa
     4981 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc
     5041 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc
     5101 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct
     5161 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga
     5221 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
     5281 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc
     5341 ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
     5401 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
     5461 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
     5521 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg
     5581 aatagttaat taagctagct aattcgatat caagcttatc gataatcaac ctctggatta
     5641 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     5701 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     5761 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     5821 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     5881 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     5941 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     6001 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     6061 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     6121 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     6181 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagacctaga
     6241 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
     6301 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc
     6361 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     6421 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca
     6481 aggctacttc cctgattggc agaactacac accagggcca gggatcagat atccactgac
     6541 ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga
     6601 aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     6661 agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct
     6721 gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6781 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6841 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6901 gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     6961 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     7021 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     7081 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     7141 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     7201 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7261 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     7321 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     7381 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     7441 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     7501 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     7561 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     7621 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     7681 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7741 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7801 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     7861 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     7921 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     7981 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt
     8041 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     8101 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg
     8161 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc
     8221 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg
     8281 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg
     8341 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag
     8401 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg
     8461 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
     8521 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     8581 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     8641 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     8701 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     8761 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     8821 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     8881 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     8941 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     9001 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     9061 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     9121 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     9181 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     9241 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     9301 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     9361 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     9421 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     9481 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     9541 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9601 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     9661 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     9721 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     9781 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     9841 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     9901 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     9961 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    10021 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    10081 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    10141 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    10201 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    10261 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    10321 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    10381 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    10441 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    10501 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    10561 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    10621 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    10681 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    10741 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    10801 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    10861 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    10921 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
    10981 caatctgctc tgatgccgca tagttaagcc agtatctgc
//
