LOCUS       sequence-380708-       10012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation, Inducible lentiviral expression, TRE-gateway; 
            PGK-rtTA-2A-blasti.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10012)
  TITLE     Superti-Furga RESOLUTE modified pLIX plasmids
REFERENCE   2  (bases 1 to 10012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCW57.1_V5_APEX_FLAG_Nt
FEATURES             Location/Qualifiers
     source          1..10012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    78..195
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        249..563
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    257..275
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    293..311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    328..346
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    364..382
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    400..418
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    435..453
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    471..489
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     536..560
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             630..1376
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             1407..1430
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1443..1567
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1592..1597,1598..1615,1616..1622)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1676..2335
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1742..1761)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2677..2982
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2861..2880
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3023..3147)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3165..3182
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        3192..3702
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3621..3639
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3712..4107
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             4108..4161
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4162..4779,4780..4785,4786..4821,4822..4824,4825..4860,
                     4861..4863,4864..4899,4900..4908)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4948..4968)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5366..5377)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5582..5815
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5887..6008
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5924..5943)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5978..5997
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6048..6183
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6183..6254
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6255..6323,6324..7115)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6473..6492)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7286..7874
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7775..7794
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8028..8045
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8162..8183
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8198..8203,8204..8221,8222..8228)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8236..8252
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8241..8263
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8260..8276
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8260..8276
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8295..8315
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8297..8315
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8343..8569
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8570..8750
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8797..8922
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9415..9648
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9833..9877
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttatcgtttc agacccacct cccaaccccg aggggacaat tctcgacctc gagacaaatg
       61 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
      121 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
      181 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gccgcgaatc
      241 gatatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc
      301 agtgatagag aacgatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
      361 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
      421 gtatgtcgag tttatcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
      481 tgatagagaa cgtatgtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc
      541 gtttagtgaa ccgtcagatc gcctggagaa ttggctagca tcaaaccatg ggacccatcc
      601 ccaaccccct gctgggcctg gacagcaccg gaaagtctta cccaactgtg agtgctgatt
      661 accaggacgc cgttgagaag gcgaagaaga agctcagagg cttcatcgct gagaagagat
      721 gcgctcctct aatgctccgt ttggcattcc actctgctgg aacctttgac aagggcacga
      781 agaccggtgg acccttcgga accatcaagc accctgccga actggctcac agcgctaaca
      841 acggtcttga catcgctgtt aggcttttgg agccactcaa ggcggagttc cctattttga
      901 gctacgccga tttctaccag ttggctggcg ttgttgccgt tgaggtcacg ggtggaccta
      961 aggttccatt ccaccctgga agagaggaca agcctgagcc accaccagag ggtcgcttgc
     1021 ctgatcccac taagggttct gaccatttga gagatgtgtt tggcaaagct atggggctta
     1081 ctgaccaaga tatcgttgct ctatctgggg gtcacactat tggagctgca cacaaggagc
     1141 gttctggatt tgagggtccc tggacctcta atcctcttat tttcgacaac tcatacttca
     1201 cggagttgtt gagtggtgag aaggaaggtc tccttcagct accttctgac aaggctcttt
     1261 tgtctgaccc tgtattccgc cctctcgttg ataaatatgc agcggacgaa gatgccttct
     1321 ttgctgatta cgctgaggct caccaaaagc tttccgagct tgggtttgct gatgccgggg
     1381 gatctggccc cggcggcggc gcgccagact acaaagacga tgacgataaa ctaggcgcgc
     1441 ccacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat
     1501 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt
     1561 cactatggcg gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa
     1621 tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga
     1681 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
     1741 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
     1801 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
     1861 tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct
     1921 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
     1981 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
     2041 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
     2101 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
     2161 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa
     2221 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg
     2281 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaagatc
     2341 tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg
     2401 tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag
     2461 cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg
     2521 tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg
     2581 aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga
     2641 acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat
     2701 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc
     2761 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt
     2821 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
     2881 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
     2941 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata
     3001 cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt
     3061 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
     3121 tcgttcagct ttcttgtaca aagtggttta gtaatgaacc ggtccaccac caccaccacc
     3181 actaaggatc cggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga
     3241 cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca
     3301 ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg
     3361 gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg
     3421 acgtgacaaa cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga
     3481 gcaatggcag cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc
     3541 gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg
     3601 gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca
     3661 gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agcaattcac catggccaag
     3721 cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc
     3781 cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc
     3841 actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc
     3901 actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac
     3961 aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg
     4021 atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg
     4081 ctgccctctg gttatgtgtg ggagggcgaa ggtagaggtt ctctcctcac ttgtggtgat
     4141 gttgaagaaa accctggtcc aatgtctaga ctggacaaga gcaaagtcat aaacggagct
     4201 ctggaattac tcaatggtgt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag
     4261 ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat
     4321 gccctgccaa tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag
     4381 tcatggcaag actttctgcg gaacaacgcc aagtcatacc gctgtgctct cctctcacat
     4441 cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg
     4501 gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct
     4561 ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga gcatcaagta
     4621 gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct gagacaagca
     4681 attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct ggaactaatc
     4741 atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac
     4801 gattttgact tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg
     4861 cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccgggtaact aagtaaggat
     4921 cgatccaaga tatcgtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     4981 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     5041 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     5101 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     5161 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     5221 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     5281 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     5341 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     5401 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     5461 ctccctttgg gccgcctccc cgcctgtttc gcctcggcgt ccggactaga ggtaccttta
     5521 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     5581 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     5641 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5701 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     5761 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     5821 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     5881 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5941 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6001 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     6061 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6121 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6181 gcctttcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6241 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6301 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6361 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6421 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6481 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6541 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6601 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6661 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6721 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6781 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6841 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6901 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6961 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7021 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7081 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7141 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7201 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7261 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7321 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7381 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7441 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7501 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7561 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7621 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7681 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7741 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7801 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7861 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7921 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7981 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8041 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     8101 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     8161 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     8221 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     8281 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     8341 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     8401 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     8461 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     8521 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     8581 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8641 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8701 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     8761 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     8821 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     8881 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     8941 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     9001 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     9061 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     9121 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     9181 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     9241 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     9301 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     9361 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     9421 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     9481 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     9541 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     9601 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     9661 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     9721 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     9781 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     9841 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     9901 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     9961 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac ca
//
