LOCUS       sequence-380709-        9968 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation, Inducible lentiviral expression, TRE-gateway; 
            PGK-rtTA-2A-blasti.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9968)
  TITLE     Superti-Furga RESOLUTE modified pLIX plasmids
REFERENCE   2  (bases 1 to 9968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCW57.1_V5_APEX_FLAG_Ct
FEATURES             Location/Qualifiers
     source          1..9968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..171
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        225..539
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    269..287
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    304..322
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    340..358
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    376..394
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    411..429
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    447..465
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     512..536
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    560..684
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(709..714,715..732,733..739)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             793..1452
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(859..878)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1794..2099
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1978..1997
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2140..2264)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2275..2298
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2329..3075
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     promoter        3124..3634
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3553..3571
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3644..4039
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             4040..4093
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4094..4711,4712..4717,4718..4753,4754..4756,4757..4792,
                     4793..4795,4796..4831,4832..4840)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4880..4900)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5298..5309)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5514..5747
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5819..5940
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5856..5875)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5910..5929
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5980..6115
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6115..6186
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6187..6255,6256..7047)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6405..6424)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7218..7806
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7707..7726
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7960..7977
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8094..8115
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8130..8135,8136..8153,8154..8160)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8168..8184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8173..8195
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8192..8208
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8192..8208
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8227..8247
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8229..8247
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8275..8501
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8502..8682
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8729..8854
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9347..9580
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9765..9809
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 accccgaggg gacaattctc gacctcgaga caaatggcag tattcatcca caattttaaa
       61 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
      121 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
      181 tacagggaca gcagagatcc actttggccg cgaatcgata tgtcgagttt actccctatc
      241 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg atgtcgagtt
      301 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
      361 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta tccctatcag
      421 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgaggta
      481 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct
      541 ggagaattgg ctagcatcaa caagtttgta caaaaaagct gaacgagaaa cgtaaaatga
      601 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa
      661 aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat
      721 gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct
      781 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      841 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      901 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      961 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
     1021 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     1081 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     1141 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     1201 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
     1261 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     1321 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
     1381 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     1441 ggcggggcgt aaagatctgg atccggctta ctaaaagcca gataacagta tgcgtatttg
     1501 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
     1561 agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
     1621 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc
     1681 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
     1741 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt
     1801 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
     1861 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
     1921 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
     1981 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
     2041 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
     2101 tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt
     2161 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
     2221 atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgattc tagagactac
     2281 aaagacgatg acgataaacc agcgggcggc ggccccggct ctggaggggg aaagtcttac
     2341 ccaactgtga gtgctgatta ccaggacgcc gttgagaagg cgaagaagaa gctcagaggc
     2401 ttcatcgctg agaagagatg cgctcctcta atgctccgtt tggcattcca ctctgctgga
     2461 acctttgaca agggcacgaa gaccggtgga cccttcggaa ccatcaagca ccctgccgaa
     2521 ctggctcaca gcgctaacaa cggtcttgac atcgctgtta ggcttttgga gccactcaag
     2581 gcggagttcc ctattttgag ctacgccgat ttctaccagt tggctggcgt tgttgccgtt
     2641 gaggtcacgg gtggacctaa ggttccattc caccctggaa gagaggacaa gcctgagcca
     2701 ccaccagagg gtcgcttgcc tgatcccact aagggttctg accatttgag agatgtgttt
     2761 ggcaaagcta tggggcttac tgaccaagat atcgttgctc tatctggggg tcacactatt
     2821 ggagctgcac acaaggagcg ttctggattt gagggtccct ggacctctaa tcctcttatt
     2881 ttcgacaact catacttcac ggagttgttg agtggtgaga aggaaggtct ccttcagcta
     2941 ccttctgaca aggctctttt gtctgaccct gtattccgcc ctctcgttga taaatatgca
     3001 gcggacgaag atgccttctt tgctgattac gctgaggctc accaaaagct ttccgagctt
     3061 gggtttgctg atgccggacc catccccaac cccctgctgg gcctggacag cacctaagga
     3121 tccggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg
     3181 ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac
     3241 gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct
     3301 tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca
     3361 aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc
     3421 agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag
     3481 cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt
     3541 cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc
     3601 cctcgttgac cgaatcaccg acctctctcc ccagcaattc accatggcca agcctttgtc
     3661 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
     3721 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
     3781 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
     3841 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
     3901 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
     3961 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
     4021 tggttatgtg tgggagggcg aaggtagagg ttctctcctc acttgtggtg atgttgaaga
     4081 aaaccctggt ccaatgtcta gactggacaa gagcaaagtc ataaacggag ctctggaatt
     4141 actcaatggt gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt
     4201 tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc
     4261 aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca
     4321 agactttctg cggaacaacg ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg
     4381 ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca
     4441 gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc
     4501 cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga
     4561 ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag caattgagct
     4621 gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg
     4681 cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg acgattttga
     4741 cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga
     4801 cgctcttgac gattttgacc ttgacatgct ccccgggtaa ctaagtaagg atcgatccaa
     4861 gatatcgtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     4921 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     4981 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     5041 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     5101 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     5161 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     5221 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     5281 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     5341 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     5401 gggccgcctc cccgcctgtt tcgcctcggc gtccggacta gaggtacctt taagaccaat
     5461 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     5521 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     5581 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     5641 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     5701 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     5761 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     5821 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5881 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5941 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     6001 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6061 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctttca
     6121 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     6181 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     6241 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     6301 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     6361 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     6421 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     6481 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     6541 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     6601 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     6661 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     6721 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     6781 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     6841 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     6901 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6961 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     7021 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     7081 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     7141 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     7201 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     7261 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     7321 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     7381 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     7441 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     7501 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     7561 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     7621 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7681 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7741 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7801 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     7861 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     7921 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     7981 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     8041 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     8101 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     8161 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     8221 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     8281 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
     8341 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
     8401 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
     8461 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
     8521 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8581 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8641 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     8701 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     8761 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     8821 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     8881 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     8941 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     9001 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     9061 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     9121 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     9181 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     9241 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     9301 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     9361 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     9421 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     9481 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     9541 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     9601 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     9661 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     9721 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     9781 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     9841 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     9901 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     9961 acctccca
//
