LOCUS       sequence-380744-        8577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GLUT5 protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8577)
  TITLE     Jun Liu Lab Plasmids 2022
REFERENCE   2  (bases 1 to 8577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6MQBI163 GLUT5
FEATURES             Location/Qualifiers
     source          1..8577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        42..345
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        346..549
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     500..520
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     546..570
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    626..788
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             792..815
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2391..2411)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2396..2725
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2576..2711
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        2773..2820
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2839..3237
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             3324..3557
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3629..3750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3666..3685)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3720..3739
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3790..3925
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3945..3964)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3946..3964)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3974..3991)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3974..3990)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3983..4005)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4132..4587
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4219..4238)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4429..4450
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4613..4717
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4718..4786,4787..5578)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4936..4955)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5749..6337
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6238..6257
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6491..6508
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6625..6646
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6661..6666,6667..6684,6685..6691)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6699..6715
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6704..6726
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6723..6739
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6723..6739
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6758..6778
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6760..6778
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6806..7032
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7033..7213
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7260..7385
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7878..8111
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8296..8340
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gtgaacggat ctcgacggta tcgataagct tgggagttcc gcgttacata acttacggta
       61 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      121 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      181 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      241 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      301 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      361 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      421 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      481 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      541 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
      601 ctccatagaa gacaccgact ctagtagcgc agaggcttgg ggcagccgag cggcagccag
      661 gccccggccc gggcctcggt tccagaaggg agaggagccc gccaaggcgc gcaagagagc
      721 gggctgcctc gcagtccgag ccggagaggg agcgcgagcc gcgccggccc cggacggcct
      781 ccgaaaccat ggattacaaa gacgatgacg acaaggaatt ctgcagatat ccaatggagc
      841 aacaggatca gagcatgaag gaagggaggc tgacgcttgt gcttgccctg gcaaccctga
      901 tagctgcctt tgggtcatcc ttccagtatg ggtacaacgt ggctgctgtc aactccccag
      961 cactgctcat gcaacaattt tacaatgaga cttactatgg taggaccggt gaattcatgg
     1021 aagacttccc cttgacgttg ctgtggtctg taaccgtgtc catgtttcca tttggagggt
     1081 ttatcggatc cctcctggtc ggccccttgg tgaataaatt tggcagaaaa ggggccttgc
     1141 tgttcaacaa catattttct atcgtgcctg cgatcttaat gggatgcagc agagtcgcca
     1201 catcatttga gcttatcatt atttccagac ttttggtggg aatatgtgca ggtgtatctt
     1261 ccaacgtggt ccccatgtac ttaggggagc tggcccctaa aaacctgcgg ggggctctcg
     1321 gggtggtgcc ccagctcttc atcactgttg gcatccttgt ggcccagatc tttggtcttc
     1381 ggaatctcct tgcaaacgta gatggctggc cgatcctgct ggggctgacc ggggtccccg
     1441 cggcgctgca gctccttctg ctgcccttct tccccgagag ccccaggtac ctgctgattc
     1501 agaagaaaga cgaagcggcc gccaagaaag ccctacagac gctgcgcggc tgggactctg
     1561 tggacaggga ggtggccgag atccggcagg aggatgaggc agagaaggcc gcgggcttca
     1621 tctccgtgct gaagctgttc cggatgcgct cgctgcgctg gcagctgctg tccatcatcg
     1681 tcctcatggg cggccagcag ctgtcgggcg tcaacgctat ctactactac gcggaccaga
     1741 tctacctgag cgccggcgtg ccggaggagc acgtgcagta cgtgacggcc ggcaccgggg
     1801 ccgtgaacgt ggtcatgacc ttctgcgccg tgttcgtggt ggagctcctg ggtcggaggc
     1861 tgctgctgct gctgggcttc tccatctgcc tcatagcctg ctgcgtgctc actgcagctc
     1921 tggcactgca ggacacagtg tcctggatgc catacatcag catcgtctgt gtcatctcct
     1981 acgtcatagg acatgccctc gggcccagtc ccatacccgc gctgctcatc actgagatct
     2041 tcctgcagtc ctctcggcca tctgccttca tggtgggggg cagtgtgcac tggctctcca
     2101 acttcaccgt gggcttgatc ttcccgttca tccaggaggg cctcggcccg tacagcttca
     2161 ttgtcttcgc cgtgatctgc ctcctcacca ccatctacat cttcttgatt gtcccggaga
     2221 ccaaggccaa gacgttcata gagatcaacc agattttcac caagatgaat aaggtgtctg
     2281 aagtgtaccc ggaaaaggag gaactgaaag agcttccacc tgtcacttcg gaacagtgac
     2341 tcgagtctag agggcccacc ggttagtaat gagtttggaa ttaattctgt ggaatgtgtg
     2401 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     2461 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     2521 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     2581 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     2641 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     2701 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     2761 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     2821 aaggtgagga actaaaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa
     2881 agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca
     2941 gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga
     3001 ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact
     3061 tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga
     3121 caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag
     3181 ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaagca
     3241 caattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     3301 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     3361 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     3421 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     3481 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     3541 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     3601 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     3661 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     3721 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     3781 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3841 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     3901 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     3961 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     4021 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     4081 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     4141 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     4201 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4261 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4321 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     4381 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4441 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     4501 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4561 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     4621 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4681 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     4741 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     4801 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     4861 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     4921 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     4981 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     5041 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     5101 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     5161 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     5221 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     5281 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     5341 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     5401 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     5461 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     5521 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     5581 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     5641 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     5701 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5761 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5821 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5881 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5941 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6001 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6061 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6121 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     6181 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6241 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6301 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     6361 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     6421 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     6481 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     6541 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     6601 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     6661 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     6721 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     6781 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
     6841 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
     6901 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
     6961 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
     7021 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7081 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7141 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7201 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     7261 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     7321 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     7381 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     7441 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     7501 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     7561 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     7621 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     7681 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     7741 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     7801 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     7861 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     7921 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     7981 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     8041 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     8101 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     8161 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     8221 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     8281 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     8341 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     8401 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     8461 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     8521 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgatta
//
