LOCUS       sequence-380749-        3624 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian cell expression of SARS-CoV-2 Spike protein with mutation 
            N317P with furin side replaced by GSAS and with 2 Proline 
            substitution at 986 and 987.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Manrique PD, Chakraborty S, Henderson R, Edwards RJ, Mansbach R, 
            Nguyen K, Stalls V, Saunders C, Mansouri K, Acharya P, Korber B, 
            Gnanakaran S
  TITLE     Network analysis uncovers the communication structure of SARS-CoV-2 
            spike protein identifying sites for immunogen design.
  JOURNAL   iScience. 2023 Jan 20;26(1):105855. doi: 10.1016/j.isci.2022.105855.
            Epub 2022 Dec 26.
  PUBMED    36590900
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2023-01-20"; volume: "26(1):105855. doi"; pages: " 
            10.1016/j.isci.2022.105855. Epub 2022 Dec 26"
COMMENT     Sequence Label: p-alpha-H-S-GSAS-2P-N317P
FEATURES             Location/Qualifiers
     source          1..3624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctgctcgtat ttgccgagct cctgcaggtc gatcagggac tcgttcaggt tcttggccac
       61 ctcgttcagt ctgtcgattt ccttttggat gttcaccacg ctggcgttaa tgccactgat
      121 gtcgcccagg tccacatcgg ggctggtgtg gtttttaaag tacttgtcca gctcttcctt
      181 aaaagagtcc agctcaggtt gcagagggtc gtacactgtg ttgttcacga ttccgatgac
      241 cacgtcacaa ttgccactca cgaaggtgtt gtcggtggtg ataatctggg gttcgtagaa
      301 gtttctttgt gtcacgaacc agtgtgtgcc attggaaacg aaaacgccct ctcttgggaa
      361 gtgggccttg ccgtcgtggc agatggcagg ggcggttgtg aaattcttct cttgggcagg
      421 cacgtatgtc acatgcagga acaccacgcc gtgcggcgcg ctctggggga agctcatcag
      481 gtgatagcct ttgccgcaga agtcaacgcg tttagactgt cccagcacac actcggacat
      541 cttggtagcg gcgagattgg cgctggctct tatttcagcg gccctgatca gctgttgggt
      601 cacgtaggtt tgcagggact gcagtctgcc tgtgatcagt ctatcgatct ggacttcggc
      661 ttctggaggg tccagtctag acaggatatc gttcagcaca gagctgatgg cgccaaaatt
      721 gctgctgagc tgcttcacca gggtgttcag ggcttgggcg ttctgattca ccacgtcctg
      781 cagtttgccc agggcgctgg cggtagagga gagggagtcc tggatcttgc cgatggcaga
      841 attgaactgg ttagcgatca gcttctggtt ttcatacagc acgttctggg tcacgccgat
      901 tccgttgaat ctataggcca tctgcatggc aaaggggatc tgcagggcgg cgccggcgcc
      961 aaatgtccac ccagatgtga tggtgccagc cagcagggca gaggtgtact gagcgatcat
     1021 ctcgtcggtc agcagaggtg ggagaactgt caggccgttg aatttctggg cgcagatcag
     1081 atctctggcg gctatgtcgc ccaggcaatc gccatactgt ttgataaagc cggcatcggc
     1141 cagggtgacc ttgttgaaca gcagatcctc gataaagctc cgcttgctag gcttagaagg
     1201 atcagggaga atctggctaa agttaaagcc gccgaagtcc ttgataggtg gggtcttgta
     1261 gatctgcttc acttgggcga acacctcctg ggtatttttg tcctgttcca cggcgattcc
     1321 ggtcagcgcc cggttcagtt gggtgcaaaa ggagccgtat tgcagcagca ggttgctgca
     1381 ttcagtgctg tcgccacaga tgtacattgt acagtccacg ctggtcttgg tcatgctcac
     1441 gggcaggatc tctgttgtga cgctaattgt aaagttggtg ggaatggcga tagagttgtt
     1501 ggaataggcc acgctgttct ctgcgcccag gctcatggtg taggcgatga tgctctggct
     1561 agctacagag gaagcagacc cagggctgtt tgtctgggtc tggtagctgg cacagatgcc
     1621 ggcgccgata gggatgtcgc actcgtaact gttattcacg tgctcggcgc cgatcagaca
     1681 tccggccctg gtctggaaca cattggagcc agtggagtac actctccaag taggtgtcag
     1741 ctgatcggcg tggatggcca caggaacctc ggtgcagtta acatcttggt acagcacagc
     1801 cacctggttg ctggtattgg tcccgggggt gatcacagaa actcctccga aggagcaggg
     1861 tgtaatatcc aggatttcca gggtctgtgg atctctgacg gcatctgtgg tgtcagcgat
     1921 atcacggccg aactgctgga aggggaggaa ctttttgttg ctttcggtca gcacgccggt
     1981 cccggtcagg ccgttgaagt tgaagttcac gcatttgttc ttaaccagat tggtggactt
     2041 cttaggaccg cagacggtgg cgggggcgtg gagtaactcg aaggacagca cgaccacccg
     2101 gtatggctgg tagccgactc cattggtagg ctggaagccg tagctttgca gagggaagta
     2161 acagttgaag ccttccaccc cgttacaagg tgtagaaccg gcctggtaga tctctgtgga
     2221 gatgtctctc tcgaaaggtt tcaggttaga cttgcgaaac agccggtaca ggtagttgta
     2281 gttgccgccc accttgctat ccaggttgtt agagttccag gcgatcacgc agcctgtaaa
     2341 atcatcaggc agcttatagt tgtagtcagc gatcttaccg gtctggcctg gtgcgatctg
     2401 ccgcacctca tcgcctctaa ttacgaagga gtcggcgtac acgttggtga agcacaggtc
     2461 attcagcttt gttggggaaa cgccgtagca cttgaaggtg ctgaaagagg cgctgttata
     2521 cagcacggag tagtcggcca cacaattgct gattctcttt ctattccagg cgtaaacgga
     2581 ggcaaatctt gtggcgttaa acacctcgcc gaaggggcag aggttggtaa tgtttggaaa
     2641 tcgcacgatg ctctctgtag gctgcacccg gaaggggctg gtctggtaga tgcccttttc
     2701 cacggtgaag gacttcaggg tgcactttgt ctcagagaga gggtccaggg cgcagtcgac
     2761 ggcgtctgtg attgtgccgt tctcgttgta cttcagcagg aaggttctag gttgcaggta
     2821 gcccacatag taggctgcag ctccggcggt ccatccgctg ctgctgtcgc caggagtcag
     2881 gtaggatcta tgcagggcca gcagggtttg gaaccgcgtt atgttgatgc ctatgggcag
     2941 gtcgaccagg ggttccaggg cgctgaagcc ctgagggagg tcccggacca ggttgatggg
     3001 tgtgtgcttg gagtagattt tgaagtagcc gtcgatgttc ttgaacacga attctctcag
     3061 gttcttgaag ttgccttgtt tcccctcaag gtccatcaga aaaggctgag agacgtactc
     3121 gaatgtgcag ttgttggcgc tgctgtagac tctgaactcg ctttccatcc agctcttgtt
     3181 gttcttgtgg tagtacacac ccaggaaagg atcattgcaa aactggaact cgcacacctt
     3241 gatcacgacg ttcgtggcat tgttcacgat caggaggctc tgtgttttgc tatccagggt
     3301 ggtgccgaag atccagcccc ggatgatgtt actcttctcg gtactggcga agtaaactcc
     3361 atcattgaat ggcaggacgg ggttatcaaa ccgcttggtt ccgtttgtgc cgctcacgtg
     3421 gatggcgtgg aaccatgtca cgttgctgaa gaacggcagg aagaggtcct gagtgctgtg
     3481 caggacgcta gatctgaaca ccttgtcagg gtagtacacg ccccgtgtaa agctgttggt
     3541 gtaggctggt gggagctggg ttcgggtggt caggttcacg cactggctgc tcaccagagg
     3601 caggagcacc aggaacacga acat
//
