LOCUS       sequence-38089-e        2695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Turowec JP, Duncan JS, French AC, Gyenis L, St Denis NA, Vilk G, 
            Litchfield DW.
  TITLE     Protein kinase CK2 is a constitutively active enzyme that promotes 
            cell survival: strategies to identify CK2 substrates and manipulate 
            its activity in mammalian cells.
  JOURNAL   Methods Enzymol. 2010;484:471-93.
  PUBMED    21036246
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2010"; volume: "484"; pages: "471-93"
COMMENT     Sequence Label: pDB1 (CK2alpha)
FEATURES             Location/Qualifiers
     source          1..2695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(183..188,189..204,205..211)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    219..235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     224..246
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             258..911
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     869..891
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             921..932
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2431..2453)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2598..2616)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
      301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
      361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
      421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
      481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
      541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
      601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
      661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
      721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
      781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
      841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
      901 atcctccaaa atcggatctg atcgaaggtc gtgggatcct gtcgggaccc gtgccaagca
      961 gggccagagt ttacacagat gttaatacac acagacctcg agaatactgg gattacgagt
     1021 cacatgtggt ggaatgggga aatcaagatg actaccagct ggttcgaaaa ttaggccgag
     1081 gtaaatacag tgaagtattt gaagccatca acatcacaaa taatgaaaaa gttgttgtta
     1141 aaattctcaa gccagtaaaa aagaagaaaa ttaagcgtga aataaagatt ttggagaatt
     1201 tgagaggagg tcccaacatc atcacactgg cagacattgt aaaagaccct gtgtcacgaa
     1261 cccccgcctt ggtttttgaa cacgtaaaca acacagactt caagcaattg taccagacgt
     1321 taacagacta tgatattcga ttttacatgt atgagattct gaaggccctg gattattgtc
     1381 acagcatggg aattatgcac agagatgtca agccccataa tgtcatgatt gatcatgagc
     1441 acagaaagct acgactaata gactggggtt tggctgagtt ttatcatcct ggccaagaat
     1501 ataatgtccg agttgcttcc cgatacttca aaggtcctga gctacttgta gactatcaga
     1561 tgtacgatta tagtttggat atgtggagtt tgggttgtat gctggcaagt atgatctttc
     1621 ggaaggagcc atttttccat ggacatgaca attatgatca gttggtgagg atagccaagg
     1681 ttctggggac agaagattta tatgactata ttgacaaata caacattgaa ttagatccac
     1741 gtttcaatga tatcttgggc agacactctc gaaagcgatg ggaacgcttt gtccacagtg
     1801 aaaatcagca ccttgtcagc cctgaggcct tggatttcct ggacaaactg ctgcgatatg
     1861 accaccagtc acggcttact gcaagagagg caatggagca cccctatttc tacactgttg
     1921 tgaaggacca ggctcgaatg ggttcatcta gcatgccagg gggcagtacg cccgtcagca
     1981 gcgccaatat gatgtcaggg atttcttcag tgccaacccc ttcacccctt ggacctctgg
     2041 caggctcacc agtgattgct gctgccaacc cccttgggat gcctgttcca gctgccgctg
     2101 gcgctcagca gtaacggccc tatctgtctc ctgatgcctg agcagaggtg ggggagtcca
     2161 ccctctcctt gatgcagctt gcgcctggcg gggaggggtg aaacacttca gaagcaccgt
     2221 gtctgaaccg ttgcttgtgg atttatagta gttcagtcat aaaaaaaaaa ttataatagg
     2281 ctgattttct tttttctttt tttttttaac tcgaactttt cataactcag gggattccct
     2341 gaaaaattac ctgcagcccg ggggatcccc gggaattcat cgtgactgac tgacgatctg
     2401 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     2461 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     2521 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtataa
     2581 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     2641 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccct
//
