LOCUS       sequence-381052-       12934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses alpha subunit of hERG1a K channel in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12934)
  AUTHORS   Mitcheson JS, Chen J, Lin M, Culberson C, Sanguinetti MC
  TITLE     A structural basis for drug-induced long QT syndrome.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):12329-33.
  PUBMED    11005845
REFERENCE   2  (bases 1 to 12934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2000-10-24"; volume: "97(22)"; pages: 
            "12329-33"
COMMENT     Sequence Label: pCEP4-hERG1a
FEATURES             Location/Qualifiers
     source          1..12934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        448..827
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        828..1031
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     982..1002
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1028..1052
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    4606..4740
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4630..4649)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4684..4703
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4883..4905)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9172..9190)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9258..9362
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9363..9431,9432..10223)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9581..9600)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10394..10982
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10883..10902
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        11413..11558
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             11601..12638
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(12636..12655)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    12680..12728
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 atggtttatg gttcgtgggg gttattattt tgggcgttgc gtggggtcag gtccacgact
       61 ggactgagca gacagaccca tggtttttgg atggcctggg catggaccgc atgtactggc
      121 gcgacacgaa caccgggcgt ctgtggctgc caaacacccc cgacccccaa aaaccaccgc
      181 gcggatttct ggcgtgccaa gctagtcgac caattctcat gtttgacagc ttatcatcgc
      241 agatccgggc aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct
      301 atacattgaa tcaatattgg caattagcca tattagtcat tggttatata gcataaatca
      361 atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg
      421 gctcatgtcc aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat
      481 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      541 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      601 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      661 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg
      721 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact
      781 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      841 ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      901 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      961 gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     1021 taagcagagc tcgtttagtg aaccgtcaga tctctagaag ctgggtacca gctgctagca
     1081 agcttctcag gatgccggtg cggaggggcc acgtcgcgcc gcagaacacc ttcctggaca
     1141 ccatcatccg caagtttgag ggccagagcc gtaagttcat catcgccaac gctcgggtgg
     1201 agaactgcgc cgtcatctac tgcaacgacg gcttctgcga gctgtgcggc tactcgcggg
     1261 ccgaggtgat gcagcgaccc tgcacctgcg acttcctgca cgggccgcgc acgcagcgcc
     1321 gcgctgccgc gcagatcgcg caggcactgc tgggcgccga ggagcgcaaa gtggaaatcg
     1381 ccttctaccg gaaagatggg agctgcttcc tatgtctggt ggatgtggtg cccgtgaaga
     1441 acgaggatgg ggctgtcatc atgttcatcc tcaatttcga ggtggtgatg gagaaggaca
     1501 tggtggggtc cccggctcat gacaccaacc accggggccc ccccaccagc tggctggccc
     1561 caggccgcgc caagaccttc cgcctgaagc tgcccgcgct gctggcgctg acggcccggg
     1621 agtcgtcggt gcggtcgggc ggcgcgggcg gcgcgggcgc cccgggggcc gtggtggtgg
     1681 acgtggacct gacgcccgcg gcacccagca gcgagtcgct ggccctggac gaagtgacag
     1741 ccatggacaa ccacgtggca gggctcgggc ccgcggagga gcggcgtgcg ctggtgggtc
     1801 ccggctctcc gccccgcagc gcgcccggcc agctcccatc gccccgggcg cacagcctca
     1861 accccgacgc ctcgggctcc agctgcagcc tggcccggac gcgctcccga gaaagctgcg
     1921 ccagcgtgcg ccgcgcctcg tcggccgacg acatcgaggc catgcgcgcc ggggtgctgc
     1981 ccccgccacc gcgccacgcc agcaccgggg ccatgcaccc actgcgcagc ggcttgctca
     2041 actccacctc ggactccgac ctcgtgcgct accgcaccat tagcaagatt ccccaaatca
     2101 ccctcaactt tgtggacctc aagggcgacc ccttcttggc ttcgcccacc agtgaccgtg
     2161 agatcatagc acctaagata aaggagcgaa cccacaatgt cactgagaag gtcacccagg
     2221 tcctgtccct gggcgccgac gtgctgcctg agtacaagct gcaggcaccg cgcatccacc
     2281 gctggaccat cctgcattac agccccttca aggccgtgtg ggactggctc atcctgctgc
     2341 tggtcatcta cacggctgtc ttcacaccct actcggctgc cttcctgctg aaggagacgg
     2401 aagaaggccc gcctgctacc gagtgtggct acgcctgcca gccgctggct gtggtggacc
     2461 tcatcgtgga catcatgttc attgtggaca tcctcatcaa cttccgcacc acctacgtca
     2521 atgccaacga ggaggtggtc agccaccccg gccgcatcgc cgtccactac ttcaagggct
     2581 ggttcctcat cgacatggtg gccgccatcc ccttcgacct gctcatcttc ggctctggct
     2641 ctgaggagct gatcgggctg ctgaagactg cgcggctgct gcggctggtg cgcgtggcgc
     2701 ggaagctgga tcgctactca gagtacggcg cggccgtgct gttcttgctc atgtgcacct
     2761 ttgcgctcat cgcgcactgg ctagcctgca tctggtacgc catcggcaac atggagcagc
     2821 cacacatgga ctcacgcatc ggctggctgc acaacctggg cgaccagata ggcaaaccct
     2881 acaacagcag cggcctgggc ggcccctcca tcaaggacaa gtatgtgacg gcgctctact
     2941 tcaccttcag cagcctcacc agtgtgggct tcggcaacgt ctctcccaac accaactcag
     3001 agaagatctt ctccatctgc gtcatgctca ttggctccct catgtatgct agcatcttcg
     3061 gcaacgtgtc ggccatcatc cagcggctgt actcgggcac agcccgctac cacacacaga
     3121 tgctgcgggt gcgggagttc atccgcttcc accagatccc caatcccctg cgccagcgcc
     3181 tcgaggagta cttccagcac gcctggtcct acaccaacgg catcgacatg aacgcggtgc
     3241 tgaagggctt ccctgagtgc ctgcaggctg acatctgcct gcacctgaac cgctcactgc
     3301 tgcagcactg caaacccttc cgaggggcca ccaagggctg ccttcgggcc ctggccatga
     3361 agttcaagac cacacatgca ccgccagggg acacactggt gcatgctggg gacctgctca
     3421 ccgccctgta cttcatctcc cggggctcca tcgagatcct gcggggcgac gtcgtcgtgg
     3481 ccatcctggg gaagaatgac atctttgggg agcctctgaa cctgtatgca aggcctggca
     3541 agtcgaacgg ggatgtgcgg gccctcacct actgtgacct acacaagatc catcgggacg
     3601 acctgctgga ggtgctggac atgtaccctg agttctccga ccacttctgg tccagcctgg
     3661 agatcacctt caacctgcga gataccaaca tgatcccggg ctcccccggc agtacggagt
     3721 tagagggtgg cttcagtcgg caacgcaagc gcaagttgtc cttccgcagg cgcacggaca
     3781 aggacacgga gcagccaggg gaggtgtcgg ccttggggcc gggccgggcg ggggcagggc
     3841 cgagtagccg gggccggccg ggggggccgt ggggggagag cccgtccagt ggcccctcca
     3901 gccctgagag cagtgaggat gagggcccag gccgcagctc cagccccctc cgcctggtgc
     3961 ccttctccag ccccaggccc cccggagagc cgccgggtgg ggagcccctg atggaggact
     4021 gcgagaagag cagcgacact tgcaaccccc tgtcaggcgc cttctcagga gtgtccaaca
     4081 ttttcagctt ctggggggac agtcggggcc gccagtacca ggagctccct cgatgccccg
     4141 cccccacccc cagcctcctc aacatccccc tctccagccc gggtcggcgg ccccggggcg
     4201 acgtggagag caggctggat gccctccagc gccagctcaa caggctggag acccggctga
     4261 gtgcagacat ggccactgtc ctgcagctgc tacagaggca gatgacgctg gtcccgcccg
     4321 cctacagtgc tgtgaccacc ccggggcctg gccccacttc cacatccccg ctgttgcccg
     4381 tcagccccct ccccaccctc accttggact cgctttctca ggtttcccag ttcatggcgt
     4441 gtgaggagct gcccccgggg gccccagagc ttccccaaga aggccccaca cgacgcctct
     4501 ccctaccggg ccagctgggg gccctcacct cccagcccct gcacagacac ggctcggacc
     4561 cgggcagtta gtggggctgc ccagtgtgga cacgtggctc acccggatcc agacatgata
     4621 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     4681 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     4741 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     4801 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatccggc tgcctcgcgc
     4861 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     4921 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     4981 ggtgtcgggg cgcagccatg aggtcgactc tagaggatcg atccccgccc cggacgaact
     5041 aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa tcccttcagt
     5101 tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg gggaccaaac
     5161 acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac atttgccaac
     5221 actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac acaacattgc
     5281 ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat gtacctccca
     5341 ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc tgtgtagcta
     5401 ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc ccttgttaac
     5461 cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac taaccctaat
     5521 tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt agtaaaaggg
     5581 tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt tacatggggt
     5641 caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg aagatcaagg
     5701 agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc gtttagctaa
     5761 tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt
     5821 caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat tgtgctatga
     5881 caccaatata accctcacaa accccttggg caataaatac tagtgtagga atgaaacatt
     5941 ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag actggatgtc
     6001 catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata tgatactggg
     6061 gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt tacactctat
     6121 ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg ttgtctctaa
     6181 cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa gacaagtggc
     6241 cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac gccgccctgc
     6301 ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc gctaaccact
     6361 gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac ctgcataagt
     6421 aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa ttacacacac
     6481 ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt cgctgagagc
     6541 acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga tagcatatgc
     6601 tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     6661 tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc
     6721 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     6781 tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg tagtatatgc
     6841 tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc atatgctatc
     6901 ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     6961 ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc
     7021 ctcatgcata tacagtcagc atatgatacc cagtagtaga gtgggagtgc tatcctttgc
     7081 atatgccgcc acctcccaag ggggcgtgaa ttttcgctgc ttgtcctttt cctgctggtt
     7141 gctcccattc ttaggtgaat ttaaggaggc caggctaaag ccgtcgcatg tctgattgct
     7201 caccaggtaa atgtcgctaa tgttttccaa cgcgagaagg tgttgagcgc ggagctgagt
     7261 gacgtgacaa catgggtatg cccaattgcc ccatgttggg aggacgaaaa tggtgacaag
     7321 acagatggcc agaaatacac caacagcacg catgatgtct actggggatt tattctttag
     7381 tgcgggggaa tacacggctt ttaatacgat tgagggcgtc tcctaacaag ttacatcact
     7441 cctgcccttc ctcaccctca tctccatcac ctccttcatc tccgtcatct ccgtcatcac
     7501 cctccgcggc agccccttcc accataggtg gaaaccaggg aggcaaatct actccatcgt
     7561 caaagctgca cacagtcacc ctgatattgc aggtaggagc gggctttgtc ataacaaggt
     7621 ccttaatcgc atccttcaaa acctcagcaa atatatgagt ttgtaaaaag accatgaaat
     7681 aacagacaat ggactccctt agcgggccag gttgtgggcc gggtccaggg gccattccaa
     7741 aggggagacg actcaatggt gtaagacgac attgtggaat agcaagggca gttcctcgcc
     7801 ttaggttgta aagggaggtc ttactacctc catatacgaa cacaccggcg acccaagttc
     7861 cttcgtcggt agtcctttct acgtgactcc tagccaggag agctcttaaa ccttctgcaa
     7921 tgttctcaaa tttcgggttg gaacctcctt gaccacgatg ctttccaaac caccctcctt
     7981 ttttgcgcct gcctccatca ccctgacccc ggggtccagt gcttgggcct tctcctgggt
     8041 catctgcggg gccctgctct atcgctcccg ggggcacgtc aggctcacca tctgggccac
     8101 cttcttggtg gtattcaaaa taatcggctt cccctacagg gtggaaaaat ggccttctac
     8161 ctggaggggg cctgcgcggt ggagacccgg atgatgatga ctgactactg ggactcctgg
     8221 gcctcttttc tccacgtcca cgacctctcc ccctggctct ttcacgactt ccccccctgg
     8281 ctctttcacg tcctctaccc cggcggcctc cactacctcc tcgaccccgg cctccactac
     8341 ctcctcgacc ccggcctcca ctgcctcctc gaccccggcc tccacctcct gctcctgccc
     8401 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc tcctgcccct cccgctcctg
     8461 ctcctgctcc tgttccaccg tgggtccctt tgcagccaat gcaacttgga cgtttttggg
     8521 gtctccggac accatctcta tgtcttggcc ctgatcctga gccgcccggg gctcctggtc
     8581 ttccgcctcc tcgtcctcgt cctcttcccc gtcctcgtcc atggttatca ccccctcttc
     8641 tttgaggtcc actgccgccg gagccttctg gtccagatgt gtctcccttc tctcctaggc
     8701 catttccagg tcctgtacct ggcccctcgt cagacatgat tcacactaaa agagatcaat
     8761 agacatcttt attagacgac gctcagtgaa tacagggagt gcagactcct gccccctcca
     8821 acagcccccc caccctcatc cccttcatgg tcgctgtcag acagatccag gtctgaaaat
     8881 tccccatcct ccgaaccatc ctcgtcctca tcaccaatta ctcgcagccc ggaaaactcc
     8941 cgctgaacat cctcaagatt tgcgtcctga gcctcaagcc aggcctcaaa ttcctcgtcc
     9001 ccctttttgc tggacggtag ggatggggat tctcgggacc cctcctcttc ctcttcaagg
     9061 tcaccagaca gagatgctac tggggcaacg gaagaaaagc tgggtgcggc ctgtgaggat
     9121 cagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga
     9181 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     9241 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     9301 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     9361 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     9421 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     9481 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     9541 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     9601 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     9661 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     9721 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     9781 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     9841 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     9901 tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     9961 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    10021 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    10081 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    10141 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    10201 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    10261 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    10321 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    10381 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    10441 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    10501 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
    10561 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    10621 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    10681 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    10741 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    10801 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    10861 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    10921 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    10981 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tgaagctgtc
    11041 cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc
    11101 cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca
    11161 gcaagacgta gcccagcgcg tcggccccga gatgcgccgc gtgcggctgc tggagatggc
    11221 ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa
    11281 ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ctgcttcatc
    11341 cccgtggccc gttgctcgcg tttgctggcg gtgtccccgg aagaaatata tttgcatgtc
    11401 tttagttcta tgatgacaca aaccccgccc agcgtcttgt cattggcgaa ttcgaacacg
    11461 cagatgcagt cggggcggcg cggtccgagg tccacttcgc atattaaggt gacgcgtgtg
    11521 gcctcgaaca ccgagcgacc ctgcagcgac ccgcttaaca gcgtcaacag cgtgccgcag
    11581 atcccggggg gcaatgagat atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
    11641 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
    11701 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
    11761 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
    11821 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
    11881 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
    11941 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
    12001 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
    12061 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
    12121 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
    12181 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
    12241 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
    12301 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
    12361 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
    12421 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
    12481 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
    12541 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
    12601 gcactcgtcc ggatcgggag atgggggagg ctaactgaaa cacggaagga gacaataccg
    12661 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc
    12721 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
    12781 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
    12841 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagccactgg
    12901 ccccgtgggt tagggacggg gtcccccatg ggga
//
