LOCUS       sequence-381217-        9065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 
            promoter driven guide RNA that can be cloned with BbsI sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   Haiting Ma, Rudolf Jaenisch
  TITLE     Multiplex genome editing of human pluripotent stem cells using Cpf1
  JOURNAL   bioRxiv
REFERENCE   2  (bases 1 to 9065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv"
COMMENT     Sequence Label: CMV:AsCpf1-2A-GFP-U6-sgRNA-cloning vector
FEATURES             Location/Qualifiers
     source          1..9065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        293..368
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        465..750
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        752..1029
                     /label=chicken beta-actin promoter
     intron          1030..1257
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      1273..1282
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1285..1305
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1330..5247
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      4967..4976
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5248..5295
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5311..5364
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5365..5367,5368..6078)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5365..5367,5368..6078)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5407..5428)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5668..5687)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6015..6036
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6106..6123)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6112..6319
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6328..6468
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6328..6457
                     /label=AAV2 ITR
     rep_origin      6543..6998
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6630..6649)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6840..6861
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7015..7034)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7134..7156
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7194..7212)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7280..7384
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7385..7453,7454..8245)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7603..7622)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8416..9004
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8905..8924
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg taatttctac tcttgtagat gtcttcgaga agactttttt ttgttttaga
      301 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
      361 gtcggtgctt ttttgtttta gagctagaaa tagcaagtta aaataaggct agtccgtttt
      421 tagcgcgtgc gccaattctg cagacaaatg gctctagagg tacccgttac ataacttacg
      481 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aatagtaacg
      541 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      601 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      661 tggcccgcct ggcattgtgc ccagtacatg accttatggg actttcctac ttggcagtac
      721 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
      781 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
      841 tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc crggsggggs ggggsggggs
      901 gggggggggg gsggggsgag gcggrgaggt gcggcggcag ccaatcagag cggcgcgctc
      961 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
     1021 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
     1081 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     1141 ccttctcctc cgggctgtaa ttagctgagc aagaggtaag ggtttaaggg atggttggtt
     1201 ggtggggtat taatgtttaa ttacctggag cacctgcctg aaatcacttt ttttcaggtt
     1261 ggaccggtgg ccgccaccat ggccccaaag aagaagcgga aggtcggtat ccacggagtc
     1321 ccagcagcca cacagttcga gggctttacc aacctgtatc aggtgagcaa gacactgcgg
     1381 tttgagctga tcccacaggg caagaccctg aagcacatcc aggagcaggg cttcatcgag
     1441 gaggacaagg cccgcaatga tcactacaag gagctgaagc ccatcatcga tcggatctac
     1501 aagacctatg ccgaccagtg cctgcagctg gtgcagctgg attgggagaa cctgagcgcc
     1561 gccatcgact cctatagaaa ggagaaaacc gaggagacaa ggaacgccct gatcgaggag
     1621 caggccacat atcgcaatgc catccacgac tacttcatcg gccggacaga caacctgacc
     1681 gatgccatca ataagagaca cgccgagatc tacaagggcc tgttcaaggc cgagctgttt
     1741 aatggcaagg tgctgaagca gctgggcacc gtgaccacaa ccgagcacga gaacgccctg
     1801 ctgcggagct tcgacaagtt tacaacctac ttctccggct tttatgagaa caggaagaac
     1861 gtgttcagcg ccgaggatat cagcacagcc atcccacacc gcatcgtgca ggacaacttc
     1921 cccaagttta aggagaattg tcacatcttc acacgcctga tcaccgccgt gcccagcctg
     1981 cgggagcact ttgagaacgt gaagaaggcc atcggcatct tcgtgagcac ctccatcgag
     2041 gaggtgtttt ccttcccttt ttataaccag ctgctgacac agacccagat cgacctgtat
     2101 aaccagctgc tgggaggaat ctctcgggag gcaggcaccg agaagatcaa gggcctgaac
     2161 gaggtgctga atctggccat ccagaagaat gatgagacag cccacatcat cgcctccctg
     2221 ccacacagat tcatccccct gtttaagcag atcctgtccg ataggaacac cctgtctttc
     2281 atcctggagg agtttaagag cgacgaggaa gtgatccagt ccttctgcaa gtacaagaca
     2341 ctgctgagaa acgagaacgt gctggagaca gccgaggccc tgtttaacga gctgaacagc
     2401 atcgacctga cacacatctt catcagccac aagaagctgg agacaatcag cagcgccctg
     2461 tgcgaccact gggatacact gaggaatgcc ctgtatgagc ggagaatctc cgagctgaca
     2521 ggcaagatca ccaagtctgc caaggagaag gtgcagcgca gcctgaagca cgaggatatc
     2581 aacctgcagg agatcatctc tgccgcaggc aaggagctga gcgaggcctt caagcagaaa
     2641 accagcgaga tcctgtccca cgcacacgcc gccctggatc agccactgcc tacaaccctg
     2701 aagaagcagg aggagaagga gatcctgaag tctcagctgg acagcctgct gggcctgtac
     2761 cacctgctgg actggtttgc cgtggatgag tccaacgagg tggaccccga gttctctgcc
     2821 cggctgaccg gcatcaagct ggagatggag ccttctctga gcttctacaa caaggccaga
     2881 aattatgcca ccaagaagcc ctactccgtg gagaagttca agctgaactt tcagatgcct
     2941 acactggcct ctggctggga cgtgaataag gagaagaaca atggcgccat cctgtttgtg
     3001 aagaacggcc tgtactatct gggcatcatg ccaaagcaga agggcaggta taaggccctg
     3061 agcttcgagc ccacagagaa aaccagcgag ggctttgata agatgtacta tgactacttc
     3121 cctgatgccg ccaagatgat cccaaagtgc agcacccagc tgaaggccgt gacagcccac
     3181 tttcagaccc acacaacccc catcctgctg tccaacaatt tcatcgagcc tctggagatc
     3241 acaaaggaga tctacgacct gaacaatcct gagaaggagc caaagaagtt tcagacagcc
     3301 tacgccaaga aaaccggcga ccagaagggc tacagagagg ccctgtgcaa gtggatcgac
     3361 ttcacaaggg attttctgtc caagtatacc aagacaacct ctatcgatct gtctagcctg
     3421 cggccatcct ctcagtataa ggacctgggc gagtactatg ccgagctgaa tcccctgctg
     3481 taccacatca gcttccagag aatcgccgag aaggagatca tggatgccgt ggagacaggc
     3541 aagctgtacc tgttccagat ctataacaag gactttgcca agggccacca cggcaagcct
     3601 aatctgcaca cactgtattg gaccggcctg ttttctccag agaacctggc caagacaagc
     3661 atcaagctga atggccaggc cgagctgttc taccgcccta agtccaggat gaagaggatg
     3721 gcacaccggc tgggagagaa gatgctgaac aagaagctga aggatcagaa aaccccaatc
     3781 cccgacaccc tgtaccagga gctgtacgac tatgtgaatc acagactgtc ccacgacctg
     3841 tctgatgagg ccagggccct gctgcccaac gtgatcacca aggaggtgtc tcacgagatc
     3901 atcaaggata ggcgctttac cagcgacaag ttctttttcc acgtgcctat cacactgaac
     3961 tatcaggccg ccaattcccc atctaagttc aaccagaggg tgaatgccta cctgaaggag
     4021 caccccgaga cacctatcat cggcatcgat cggggcgaga gaaacctgat ctatatcaca
     4081 gtgatcgact ccaccggcaa gatcctggag cagcggagcc tgaacaccat ccagcagttt
     4141 gattaccaga agaagctgga caacagggag aaggagaggg tggcagcaag gcaggcctgg
     4201 tctgtggtgg gcacaatcaa ggatctgaag cagggctatc tgagccaggt catccacgag
     4261 atcgtggacc tgatgatcca ctaccaggcc gtggtggtgc tggagaacct gaatttcggc
     4321 tttaagagca agaggaccgg catcgccgag aaggccgtgt accagcagtt cgagaagatg
     4381 ctgatcgata agctgaattg cctggtgctg aaggactatc cagcagagaa agtgggaggc
     4441 gtgctgaacc cataccagct gacagaccag ttcacctcct ttgccaagat gggcacccag
     4501 tctggcttcc tgttttacgt gcctgcccca tatacatcta agatcgatcc cctgaccggc
     4561 ttcgtggacc ccttcgtgtg gaaaaccatc aagaatcacg agagccgcaa gcacttcctg
     4621 gagggcttcg actttctgca ctacgacgtg aaaaccggcg acttcatcct gcactttaag
     4681 atgaacagaa atctgtcctt ccagaggggc ctgcccggct ttatgcctgc atgggatatc
     4741 gtgttcgaga agaacgagac acagtttgac gccaagggca cccctttcat cgccggcaag
     4801 agaatcgtgc cagtgatcga gaatcacaga ttcaccggca gataccggga cctgtatcct
     4861 gccaacgagc tgatcgccct gctggaggag aagggcatcg tgttcaggga tggctccaac
     4921 atcctgccaa agctgctgga gaatgacgat tctcacgcca tcgacaccat ggtggccctg
     4981 atccgcagcg tgctgcagat gcggaactcc aatgccgcca caggcgagga ctatatcaac
     5041 agccccgtgc gcgatctgaa tggcgtgtgc ttcgactccc ggtttcagaa cccagagtgg
     5101 cccatggacg ccgatgccaa tggcgcctac cacatcgccc tgaagggcca gctgctgctg
     5161 aatcacctga aggagagcaa ggatctgaag ctgcagaacg gcatctccaa tcaggactgg
     5221 ctggcctaca tccaggagct gcgcaacaaa aggccggcgg ccacgaaaaa ggccggccag
     5281 gcaaaaaaga aaaaggaatt cggcagtgga gagggcagag gaagtctgct aacatgcggt
     5341 gacgtcgagg agaatcctgg cccagtgagc aagggcgagg agctgttcac cggggtggtg
     5401 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     5461 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     5521 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     5581 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     5641 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     5701 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     5761 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     5821 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     5881 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     5941 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     6001 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     6061 atggacgagc tgtacaagga attctaacta gagctcgctg atcagcctcg actgtgcctt
     6121 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     6181 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     6241 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaga
     6301 atagcaggca tgctggggag cggccgcagg aacccctagt gatggagttg gccactccct
     6361 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
     6421 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc
     6481 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
     6541 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     6601 cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     6661 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     6721 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
     6781 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     6841 tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt
     6901 ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt
     6961 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
     7021 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     7081 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     7141 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     7201 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     7261 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7321 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7381 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7441 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7501 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7561 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7621 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7681 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7741 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7801 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7861 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7921 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7981 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8041 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8101 aagccgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8161 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8221 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8281 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8341 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8401 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8461 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8521 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8581 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8641 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8701 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8761 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8821 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8881 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8941 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     9001 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9061 catgt
//
