LOCUS       sequence-381224-        9059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 
            promoter driven guide RNA that target hINS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9059)
  AUTHORS   Haiting Ma, Rudolf Jaenisch
  TITLE     Multiplex genome editing of human pluripotent stem cells using Cpf1
  JOURNAL   bioRxiv
REFERENCE   2  (bases 1 to 9059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv"
COMMENT     Sequence Label: CMV:AsCpf1-2A-GFP-U6-INS-sg
FEATURES             Location/Qualifiers
     source          1..9059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          127..354
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      370..379
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             382..402
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             427..4344
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      4064..4073
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4345..4392
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4408..4461
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4462..4464,4465..5175)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4462..4464,4465..5175)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4504..4525)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4765..4784)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5112..5133
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5203..5220)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5209..5416
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5425..5565
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5425..5554
                     /label=AAV2 ITR
     rep_origin      5640..6095
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5727..5746)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5937..5958
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6112..6131)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6231..6253
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6291..6309)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6377..6481
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6482..6550,6551..7342)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6700..6719)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7513..8101
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8002..8021
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8163..8411
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8163..8183
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8334..8353
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8463..8538
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        8635..8920
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     gap             9039..9055
                     /estimated_length=17
ORIGIN
        1 gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga
       61 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
      121 cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc
      181 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct
      241 tctcctccgg gctgtaatta gctgagcaag aggtaagggt ttaagggatg gttggttggt
      301 ggggtattaa tgtttaatta cctggagcac ctgcctgaaa tcactttttt tcaggttgga
      361 ccggtggccg ccaccatggc cccaaagaag aagcggaagg tcggtatcca cggagtccca
      421 gcagccacac agttcgaggg ctttaccaac ctgtatcagg tgagcaagac actgcggttt
      481 gagctgatcc cacagggcaa gaccctgaag cacatccagg agcagggctt catcgaggag
      541 gacaaggccc gcaatgatca ctacaaggag ctgaagccca tcatcgatcg gatctacaag
      601 acctatgccg accagtgcct gcagctggtg cagctggatt gggagaacct gagcgccgcc
      661 atcgactcct atagaaagga gaaaaccgag gagacaagga acgccctgat cgaggagcag
      721 gccacatatc gcaatgccat ccacgactac ttcatcggcc ggacagacaa cctgaccgat
      781 gccatcaata agagacacgc cgagatctac aagggcctgt tcaaggccga gctgtttaat
      841 ggcaaggtgc tgaagcagct gggcaccgtg accacaaccg agcacgagaa cgccctgctg
      901 cggagcttcg acaagtttac aacctacttc tccggctttt atgagaacag gaagaacgtg
      961 ttcagcgccg aggatatcag cacagccatc ccacaccgca tcgtgcagga caacttcccc
     1021 aagtttaagg agaattgtca catcttcaca cgcctgatca ccgccgtgcc cagcctgcgg
     1081 gagcactttg agaacgtgaa gaaggccatc ggcatcttcg tgagcacctc catcgaggag
     1141 gtgttttcct tcccttttta taaccagctg ctgacacaga cccagatcga cctgtataac
     1201 cagctgctgg gaggaatctc tcgggaggca ggcaccgaga agatcaaggg cctgaacgag
     1261 gtgctgaatc tggccatcca gaagaatgat gagacagccc acatcatcgc ctccctgcca
     1321 cacagattca tccccctgtt taagcagatc ctgtccgata ggaacaccct gtctttcatc
     1381 ctggaggagt ttaagagcga cgaggaagtg atccagtcct tctgcaagta caagacactg
     1441 ctgagaaacg agaacgtgct ggagacagcc gaggccctgt ttaacgagct gaacagcatc
     1501 gacctgacac acatcttcat cagccacaag aagctggaga caatcagcag cgccctgtgc
     1561 gaccactggg atacactgag gaatgccctg tatgagcgga gaatctccga gctgacaggc
     1621 aagatcacca agtctgccaa ggagaaggtg cagcgcagcc tgaagcacga ggatatcaac
     1681 ctgcaggaga tcatctctgc cgcaggcaag gagctgagcg aggccttcaa gcagaaaacc
     1741 agcgagatcc tgtcccacgc acacgccgcc ctggatcagc cactgcctac aaccctgaag
     1801 aagcaggagg agaaggagat cctgaagtct cagctggaca gcctgctggg cctgtaccac
     1861 ctgctggact ggtttgccgt ggatgagtcc aacgaggtgg accccgagtt ctctgcccgg
     1921 ctgaccggca tcaagctgga gatggagcct tctctgagct tctacaacaa ggccagaaat
     1981 tatgccacca agaagcccta ctccgtggag aagttcaagc tgaactttca gatgcctaca
     2041 ctggcctctg gctgggacgt gaataaggag aagaacaatg gcgccatcct gtttgtgaag
     2101 aacggcctgt actatctggg catcatgcca aagcagaagg gcaggtataa ggccctgagc
     2161 ttcgagccca cagagaaaac cagcgagggc tttgataaga tgtactatga ctacttccct
     2221 gatgccgcca agatgatccc aaagtgcagc acccagctga aggccgtgac agcccacttt
     2281 cagacccaca caacccccat cctgctgtcc aacaatttca tcgagcctct ggagatcaca
     2341 aaggagatct acgacctgaa caatcctgag aaggagccaa agaagtttca gacagcctac
     2401 gccaagaaaa ccggcgacca gaagggctac agagaggccc tgtgcaagtg gatcgacttc
     2461 acaagggatt ttctgtccaa gtataccaag acaacctcta tcgatctgtc tagcctgcgg
     2521 ccatcctctc agtataagga cctgggcgag tactatgccg agctgaatcc cctgctgtac
     2581 cacatcagct tccagagaat cgccgagaag gagatcatgg atgccgtgga gacaggcaag
     2641 ctgtacctgt tccagatcta taacaaggac tttgccaagg gccaccacgg caagcctaat
     2701 ctgcacacac tgtattggac cggcctgttt tctccagaga acctggccaa gacaagcatc
     2761 aagctgaatg gccaggccga gctgttctac cgccctaagt ccaggatgaa gaggatggca
     2821 caccggctgg gagagaagat gctgaacaag aagctgaagg atcagaaaac cccaatcccc
     2881 gacaccctgt accaggagct gtacgactat gtgaatcaca gactgtccca cgacctgtct
     2941 gatgaggcca gggccctgct gcccaacgtg atcaccaagg aggtgtctca cgagatcatc
     3001 aaggataggc gctttaccag cgacaagttc tttttccacg tgcctatcac actgaactat
     3061 caggccgcca attccccatc taagttcaac cagagggtga atgcctacct gaaggagcac
     3121 cccgagacac ctatcatcgg catcgatcgg ggcgagagaa acctgatcta tatcacagtg
     3181 atcgactcca ccggcaagat cctggagcag cggagcctga acaccatcca gcagtttgat
     3241 taccagaaga agctggacaa cagggagaag gagagggtgg cagcaaggca ggcctggtct
     3301 gtggtgggca caatcaagga tctgaagcag ggctatctga gccaggtcat ccacgagatc
     3361 gtggacctga tgatccacta ccaggccgtg gtggtgctgg agaacctgaa tttcggcttt
     3421 aagagcaaga ggaccggcat cgccgagaag gccgtgtacc agcagttcga gaagatgctg
     3481 atcgataagc tgaattgcct ggtgctgaag gactatccag cagagaaagt gggaggcgtg
     3541 ctgaacccat accagctgac agaccagttc acctcctttg ccaagatggg cacccagtct
     3601 ggcttcctgt tttacgtgcc tgccccatat acatctaaga tcgatcccct gaccggcttc
     3661 gtggacccct tcgtgtggaa aaccatcaag aatcacgaga gccgcaagca cttcctggag
     3721 ggcttcgact ttctgcacta cgacgtgaaa accggcgact tcatcctgca ctttaagatg
     3781 aacagaaatc tgtccttcca gaggggcctg cccggcttta tgcctgcatg ggatatcgtg
     3841 ttcgagaaga acgagacaca gtttgacgcc aagggcaccc ctttcatcgc cggcaagaga
     3901 atcgtgccag tgatcgagaa tcacagattc accggcagat accgggacct gtatcctgcc
     3961 aacgagctga tcgccctgct ggaggagaag ggcatcgtgt tcagggatgg ctccaacatc
     4021 ctgccaaagc tgctggagaa tgacgattct cacgccatcg acaccatggt ggccctgatc
     4081 cgcagcgtgc tgcagatgcg gaactccaat gccgccacag gcgaggacta tatcaacagc
     4141 cccgtgcgcg atctgaatgg cgtgtgcttc gactcccggt ttcagaaccc agagtggccc
     4201 atggacgccg atgccaatgg cgcctaccac atcgccctga agggccagct gctgctgaat
     4261 cacctgaagg agagcaagga tctgaagctg cagaacggca tctccaatca ggactggctg
     4321 gcctacatcc aggagctgcg caacaaaagg ccggcggcca cgaaaaaggc cggccaggca
     4381 aaaaagaaaa aggaattcgg cagtggagag ggcagaggaa gtctgctaac atgcggtgac
     4441 gtcgaggaga atcctggccc agtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     4501 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     4561 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     4621 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     4681 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     4741 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     4801 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     4861 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     4921 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     4981 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     5041 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     5101 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     5161 gacgagctgt acaaggaatt ctaactagag ctcgctgatc agcctcgact gtgccttcta
     5221 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     5281 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     5341 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagagaata
     5401 gcaggcatgc tggggagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc
     5461 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     5521 cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt
     5581 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
     5641 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     5701 tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     5761 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     5821 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     5881 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     5941 actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata
     6001 agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     6061 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
     6121 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     6181 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     6241 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     6301 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     6361 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6421 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6481 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6541 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6601 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6661 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6721 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6781 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6841 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6901 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6961 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7021 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7081 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7141 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggaag
     7201 ccgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7261 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7321 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7381 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7441 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7501 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7561 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7621 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7681 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7741 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7801 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7861 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7921 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7981 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8041 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8101 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8161 gtgagggcct atttcccatg attccttcat atttgcatat acgatacaag gctgttagag
     8221 agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta
     8281 gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc
     8341 atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     8401 gacgaaacac cgtaatttct actcttgtag atttccatct ctctcggtgc aggaggtttt
     8461 ttgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
     8521 gtggcaccga gtcggtgctt ttttgtttta gagctagaaa tagcaagtta aaataaggct
     8581 agtccgtttt tagcgcgtgc gccaattctg cagacaaatg gctctagagg tacccgttac
     8641 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     8701 aatagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     8761 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     8821 tgacggtaaa tggcccgcct ggcattgtgc ccagtacatg accttatggg actttcctac
     8881 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
     8941 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
     9001 ttaattattt tgtgcagcga tgggggcggg ggggggggnn nnnnnnnnnn nnnnngggc
//
