LOCUS       sequence-381225-        9265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  px458 with guide RNA that target hMAFB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9265)
  AUTHORS   Haiting Ma, Rudolf Jaenisch
  TITLE     Multiplex genome editing of human pluripotent stem cells using Cpf1
  JOURNAL   bioRxiv
REFERENCE   2  (bases 1 to 9265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv"
COMMENT     Sequence Label: pX458-MAFB-sg
FEATURES             Location/Qualifiers
     source          1..9265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          135..362
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      374..383
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             383..448
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             455..475
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             500..4600
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             500..4600
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4601..4648
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4664..4717
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4718..4720,4721..5431)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4718..4720,4721..5431)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5021..5040)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5368..5389
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5459..5476)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5465..5672
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5681..5821
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5681..5810
                     /label=AAV2 ITR
     rep_origin      5896..6351
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5983..6002)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6193..6214
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6368..6387)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6487..6509
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6547..6565)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6633..6737
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6738..6806,6807..7598)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6956..6975)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7769..8357
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8258..8277
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8419..8667
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8419..8439
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8590..8609
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8689..8764
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        8861..9146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 ggggcnnngg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg
       61 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa
      121 gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg
      181 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg
      241 acggcccttc tcctccgggc tgtaattagc tgagcaagag gtaagggttt aagggatggt
      301 tggttggtgg ggtattaatg tttaattacc tggagcacct gcctgaaatc actttttttc
      361 aggttggacc ggtgccacca tggactataa ggaccacgac ggagactaca aggatcatga
      421 tattgattac aaagacgatg acgataagat ggccccaaag aagaagcgga aggtcggtat
      481 ccacggagtc ccagcagccg acaagaagta cagcatcggc ctggacatcg gcaccaactc
      541 tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct
      601 gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag
      661 cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg
      721 gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga
      781 cagcttcttc cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg
      841 gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat
      901 ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta
      961 tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa
     1021 ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct
     1081 gttcgaggaa aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag
     1141 actgagcaag agcagacggc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa
     1201 tggcctgttc ggaaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa
     1261 cttcgacctg gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct
     1321 ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa
     1381 cctgtccgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc
     1441 ccccctgagc gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct
     1501 gaaagctctc gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag
     1561 caagaacggc tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt
     1621 catcaagccc atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaacag
     1681 agaggacctg ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca
     1741 cctgggagag ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga
     1801 caaccgggaa aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct
     1861 ggccagggga aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc
     1921 ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat
     1981 gaccaacttc gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta
     2041 cgagtacttc accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag
     2101 aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac
     2161 caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt
     2221 cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca
     2281 cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat
     2341 tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg
     2401 gctgaaaacc tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag
     2461 atacaccggc tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc
     2521 cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca
     2581 gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg
     2641 ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa
     2701 gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa
     2761 gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa
     2821 gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat
     2881 cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta
     2941 cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga
     3001 ctacgatgtg gaccatatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa
     3061 ggtgctgacc agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt
     3121 cgtgaagaag atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag
     3181 aaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg
     3241 cttcatcaag agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct
     3301 ggactcccgg atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt
     3361 gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt
     3421 gcgcgagatc aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac
     3481 cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt
     3541 gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa
     3601 gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg
     3661 cgagatccgg aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga
     3721 taagggccgg gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt
     3781 gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa
     3841 cagcgataag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga
     3901 cagccccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa
     3961 gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga
     4021 gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat
     4081 catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc
     4141 ctctgccggc gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt
     4201 cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa
     4261 acagctgttt gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga
     4321 gttctccaag agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa
     4381 caagcaccgg gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct
     4441 gaccaatctg ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag
     4501 gtacaccagc accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct
     4561 gtacgagaca cggatcgacc tgtctcagct gggaggcgac aaaaggccgg cggccacgaa
     4621 aaaggccggc caggcaaaaa agaaaaagga attcggcagt ggagagggca gaggaagtct
     4681 gctaacatgc ggtgacgtcg aggagaatcc tggcccagtg agcaagggcg aggagctgtt
     4741 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     4801 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     4861 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     4921 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     4981 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     5041 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     5101 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     5161 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     5221 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     5281 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     5341 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     5401 gatcactctc ggcatggacg agctgtacaa ggaattctaa ctagagctcg ctgatcagcc
     5461 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     5521 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     5581 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     5641 gattgggaag agaatagcag gcatgctggg gagcggccgc aggaacccct agtgatggag
     5701 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
     5761 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
     5821 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     5881 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     5941 cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc
     6001 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6061 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     6121 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6181 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct
     6241 attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga
     6301 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     6361 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     6421 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     6481 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     6541 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     6601 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6661 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6721 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     6781 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     6841 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     6901 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     6961 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7021 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7081 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     7141 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7201 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7261 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7321 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7381 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7441 ccggtgagcg tggaagccgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7501 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     7561 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     7621 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     7681 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     7741 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     7801 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     7861 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     7921 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     7981 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8041 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8101 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8161 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8221 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8281 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8341 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8401 ggccttttgc tcacatgtga gggcctattt cccatgattc cttcatattt gcatatacga
     8461 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta
     8521 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     8581 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
     8641 tatatcttgt ggaaaggacg aaacaccgga aagaactcgg gagaggaggt tttagagcta
     8701 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
     8761 gtgctttttt gttttagagc tagaaatagc aagttaaaat aaggctagtc cgtttttagc
     8821 gcgtgcgcca attctgcaga caaatggctc tagaggtacc cgttacataa cttacggtaa
     8881 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata gtaacgccaa
     8941 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     9001 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     9061 ccgcctggca ttgtgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     9121 acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc
     9181 ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg
     9241 cagcgatggg ggcggggggg ggggg
//
