LOCUS       sequence-381237-        8566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  knockin donor vector of mAID-mCherry2-Hygro to C-terminus of 
            endogenous human eIF3f.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8566)
  AUTHORS   Duan H, Zhang S, Zarai Y, Ollinger R, Wu Y, Sun L, Hu C, He Y, Tian 
            G, Rad R, Kong X, Cheng Y, Tuller T, Wolf DA
  TITLE     eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant 
            regulator of ribosome content.
  JOURNAL   EMBO J. 2023 Jun 15;42(12):e112362. doi: 10.15252/embj.2022112362. 
            Epub 2023 May 8.
  PUBMED    37155573
REFERENCE   2  (bases 1 to 8566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2023-06-15"; volume: "42(12):e112362. doi"; pages: " 
            10.15252/embj.2022112362. Epub 2023 May 8"
COMMENT     Sequence Label: eIF3f- pMK293 (mAID-mCherry2-Hygro) plasmid
FEATURES             Location/Qualifiers
     source          1..8566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    99..120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(135..140,141..158,159..165)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    173..189
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     178..200
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     197..213
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     197..213
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        231..249
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     231..248
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1137..1340
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             1347..2057
                     /codon_start=1
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /label=mCherry2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1492..1510)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    2175..2296
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2212..2231)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2266..2285
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    2327..2360
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2421..2920
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2456..2479)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2853..2872
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2981..4000
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             2981..4000
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     protein_bind    4563..4596
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5446..5465)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5447..5465)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5472..5489)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5472..5488)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5481..5503)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             5626..5928
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5807..5826
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             6277..7071
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6277..7071
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6331..6350)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6941..6960
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     regulatory      7271..7280
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7277..7651
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      7789..8377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8278..8297
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8531..8548
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
       61 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
      121 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      181 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct atttaggtga
      241 cactatagaa tactcaagct atgcatcaag cttggtacca ctagtaacgg ccgccagtgt
      301 gctggaattc gcccttagtc tttgttttgg tactcagtct ggaaaggtgt cagctgacaa
      361 tactgtgggc cgcttcctga tgagcctggt taaccaagta ccgaaaatag ttcccgatga
      421 ctttgagacc atgctcaaca gcaacatcaa tgtgagtgcc cttcctgagc cccttcttgc
      481 ctggtttctt cccccacctc agcacataca ctcaaatgtg aaaagagttg ggtgaggtga
      541 cctggagtag gatagagaca gggcttcacc tatagcccca ctgaagtcca ggttgggagg
      601 aaggaagagg tgtgttatgc gtggttggag acttccttcc ttctcccatg attccattgt
      661 ctcatttact gctgatacta ctgtgactta ctgtctaaat tcgtgatgga aagaaacact
      721 aaaattcaga ggttaatgaa aatgcaatta cttatttccc ccatctgagt tcacagaccc
      781 cctatattcc atccatgggt ttcagctaag gcacccctag tctataagtg tatcagtaga
      841 gataagaaag ccttctcaag catttccttt ctgtcttcta cctttttgac ttttattcag
      901 tctctcctag ctgtcattta tccacagctg tcctcatacc cagtgtctac catagagctg
      961 gccaaagcca gcctttttgc tccctttcca cccaaccccc cactcattgt gtattctttg
     1021 tcttccagga ccttttgatg gtgacctacc tggccaacct cacacaatcg caaatagctc
     1081 taaatgaaaa acttgtaaac ctgggaggag gaggaggagg atccggtgca ggcgccaagg
     1141 agaagagtgc ttgtcctaaa gatccagcca aacctccggc caaggcacaa gttgtgggat
     1201 ggccaccggt gagatcatac cggaagaacg tgatggtttc ctgccaaaaa tcaagcggtg
     1261 gcccggaggc ggcggcgttc gtgaaggtat caatggacgg agcaccgtac ttgaggaaaa
     1321 tcgatttgag gatgtataaa gctagcatgg tgagcaaggg cgaggaggat aacatggcca
     1381 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     1441 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     1501 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     1561 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     1621 tccccgaggg cttcaattgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     1681 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     1741 ccaacttccc ctccgacggc cccgtaatgc agtgtcgtac catgggctgg gaggcctcca
     1801 ctgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     1861 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     1921 tgcagctgcc cggcgcctac aacgtcgaca tcaagttgga catcctttcc cacaacgagg
     1981 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     2041 acgagctgta caagtaatct agataactga tcataatcag ccataccaca tttgtagagg
     2101 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     2161 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     2221 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     2281 tcatcaatgt atcttaacgc gtcgatcata ttcaataacc cttaatataa cttcgtataa
     2341 tgtatgctat acgaagttat taggtctgaa gaggagttta cgtccagcca agcttaggat
     2401 ctcgacctcg aaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
     2461 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
     2521 ttccacatcc accggtaggc gccaaccgac tccgttcttt ggtggcccct tcgcgccacc
     2581 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
     2641 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
     2701 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
     2761 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
     2821 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
     2881 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcatcc atctagatct
     2941 cgagcagctg aagcttaccg ctagcatgga tagatccgga aagcctgaac tcaccgcgac
     3001 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     3061 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     3121 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     3181 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
     3241 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     3301 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     3361 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     3421 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     3481 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     3541 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     3601 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     3661 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     3721 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     3781 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     3841 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     3901 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     3961 aaaccgacgc cccagcactc gtccgagggc aaaggaatag gctagccgcc cgccccacga
     4021 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tcgatgatat cagatccccg
     4081 ggatgcagaa attgatgatc tattaaacaa taaagatgtc cactaaaatg gaagtttttc
     4141 ctgtcatact ttgttaagaa gggtgagaac agagtaccta cattttgaat ggaaggattg
     4201 gagctacggg ggtgggggtg gggtgggatt agataaatgc ctgctcttta ctgaaggctc
     4261 tttactattg ctttatgata atgtttcata gttggatatc ataatttaaa caagcaaaac
     4321 caaattaagg gccagctcat tcctcccact catgatctat agatctatag atctctcgtg
     4381 ggatcattgt ttttctcttg attcccactt tgtggttcta agtactgtgg tttccaaatg
     4441 tgtcagtttc atagcctgaa gaacgagatc agcagcctct gttccacata cacttcattc
     4501 tcagtattgt tttgccaagt tctaattcca tcagaagctg gtcgagatcc ggaaccctta
     4561 atataacttc gtataatgta tgctatacga agttattagg tccctcgaag aggttcacta
     4621 ggatcctgaa tggaccccaa gcagtacact tgctggtcta ggtattaacc ccaggactca
     4681 gaagtgaagg agaaatgggt tttttgtggt cttgagtcac actgagatag tcagttgtgt
     4741 gtgactctaa taaacggagc ctaccttttg taaattaatt tcatcttatg tgagtttatt
     4801 gccgggtgga agggaggaaa atgttttaga tcacacagaa actaggaagt gaatattgta
     4861 ggtagagaag ttggttatgt gtttggcatg ttactgtttt accaaactgt tcttttggtt
     4921 ttcaatatgg aaaggtgtcc attggcaaaa caaccttttc ttgaggattc atgtgatttc
     4981 tacaggaaat gaaaataaga atccatggag aaaaaatagc taagaaaatt ggatgcttaa
     5041 tctgaactaa aagagtagag tgtgtagtca ctatcttaaa atacattaat agtaagatgg
     5101 agctctttgc cactttggtt cggggaggca gaattaagat ctgtgggtta aagattaaag
     5161 aggtttcccc aaaacttcag aaaattctta ggtgctatct gtaactcgag gatagagcct
     5221 tgagttatac tgaggtctgc cagtggctca atttaagcaa agactagatg gcatttccca
     5281 gaggccttgt aggctagagt acttgcctgt gtaacttcag acatccattt cctctggtat
     5341 cctggagttc cgcctcgcca ctgtgtgtct tagaaaaagg gcgaattctg cagatatcca
     5401 tcacactggc ggccgctcga gcatgcatct agagggccca attcgcccta tagtgagtcg
     5461 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     5521 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     5581 cgcaccgatc gcccttccca acagttgcgc agcctatacg tacggcagtt taaggtttac
     5641 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
     5701 acgccggggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
     5761 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
     5821 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc
     5881 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggcatg
     5941 agattatcaa aaaggatctt cacctagatc cttttcacgt agaaagccag tccgcagaaa
     6001 cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc
     6061 gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt
     6121 ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag
     6181 ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca
     6241 agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     6301 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     6361 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     6421 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
     6481 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     6541 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     6601 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     6661 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     6721 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     6781 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
     6841 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     6901 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     6961 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     7021 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg aattattaac
     7081 gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     7141 atcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7201 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatagca
     7261 cgtgaggagg gccaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     7321 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     7381 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     7441 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     7501 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     7561 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     7621 cgtgcacttc gtggccgagg agcaggactg acacgtgcta aaacttcatt tttaatttaa
     7681 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7741 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7801 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7861 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7921 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7981 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8041 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8101 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8161 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8221 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8281 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8341 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8401 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     8461 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     8521 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgccca
//
