LOCUS       sequence-381241-       11294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector, with guide RNA 
            array targeting hAAVS1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11294)
  AUTHORS   Haiting Ma, Rudolf Jaenisch
  TITLE     Multiplex genome editing of human pluripotent stem cells using Cpf1
  JOURNAL   bioRxiv
REFERENCE   2  (bases 1 to 11294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv"
COMMENT     Sequence Label: CAGGS-AsCpf1(D908A)-2A-GFP-U6-AAVS1-sgRNA
FEATURES             Location/Qualifiers
     source          1..11294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          226..1242
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1166..1188
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1250..1269
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1309..1329
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1354..5271
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIARGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      4991..5000
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5272..5319
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5326..5406
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             5416..5469
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5470..5472,5473..6183)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5470..5472,5473..6183)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5512..5533)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5773..5792)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6120..6141
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6211..6228)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6217..6424
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6433..6573
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6433..6562
                     /label=AAV2 ITR
     rep_origin      6648..7103
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6735..6754)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6945..6966
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7120..7139)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7239..7261
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7299..7317)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7385..7489
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7490..7558,7559..8350)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7708..7727)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8521..9109
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9010..9029
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9171..9419
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     9171..9191
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     9342..9361
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        9470..9545
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     9769..9788
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9909..10370
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     enhancer        10468..10771
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cccccatgtt tkttwttwtt ttktttttta tttwtttggg gggggggggg gggggggggg
       61 gggggggggc gcgcgccrgg sggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
      121 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
      181 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc
      241 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
      301 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
      361 cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc
      421 cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg
      481 gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg
      541 gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg
      601 tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga
      661 gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt
      721 gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc
      781 gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc
      841 ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg
      901 gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg
      961 tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc
     1021 ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg
     1081 ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc
     1141 cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga
     1201 gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
     1261 gttattgtgc tgtctcatca ttttggcaaa gaccggtcca ccatggcccc aaagaagaag
     1321 cggaaggtcg gtatccacgg agtcccagca gccacacagt tcgagggctt taccaacctg
     1381 tatcaggtga gcaagacact gcggtttgag ctgatcccac agggcaagac cctgaagcac
     1441 atccaggagc agggcttcat cgaggaggac aaggcccgca atgatcacta caaggagctg
     1501 aagcccatca tcgatcggat ctacaagacc tatgccgacc agtgcctgca gctggtgcag
     1561 ctggattggg agaacctgag cgccgccatc gactcctata gaaaggagaa aaccgaggag
     1621 acaaggaacg ccctgatcga ggagcaggcc acatatcgca atgccatcca cgactacttc
     1681 atcggccgga cagacaacct gaccgatgcc atcaataaga gacacgccga gatctacaag
     1741 ggcctgttca aggccgagct gtttaatggc aaggtgctga agcagctggg caccgtgacc
     1801 acaaccgagc acgagaacgc cctgctgcgg agcttcgaca agtttacaac ctacttctcc
     1861 ggcttttatg agaacaggaa gaacgtgttc agcgccgagg atatcagcac agccatccca
     1921 caccgcatcg tgcaggacaa cttccccaag tttaaggaga attgtcacat cttcacacgc
     1981 ctgatcaccg ccgtgcccag cctgcgggag cactttgaga acgtgaagaa ggccatcggc
     2041 atcttcgtga gcacctccat cgaggaggtg ttttccttcc ctttttataa ccagctgctg
     2101 acacagaccc agatcgacct gtataaccag ctgctgggag gaatctctcg ggaggcaggc
     2161 accgagaaga tcaagggcct gaacgaggtg ctgaatctgg ccatccagaa gaatgatgag
     2221 acagcccaca tcatcgcctc cctgccacac agattcatcc ccctgtttaa gcagatcctg
     2281 tccgatagga acaccctgtc tttcatcctg gaggagttta agagcgacga ggaagtgatc
     2341 cagtccttct gcaagtacaa gacactgctg agaaacgaga acgtgctgga gacagccgag
     2401 gccctgttta acgagctgaa cagcatcgac ctgacacaca tcttcatcag ccacaagaag
     2461 ctggagacaa tcagcagcgc cctgtgcgac cactgggata cactgaggaa tgccctgtat
     2521 gagcggagaa tctccgagct gacaggcaag atcaccaagt ctgccaagga gaaggtgcag
     2581 cgcagcctga agcacgagga tatcaacctg caggagatca tctctgccgc aggcaaggag
     2641 ctgagcgagg ccttcaagca gaaaaccagc gagatcctgt cccacgcaca cgccgccctg
     2701 gatcagccac tgcctacaac cctgaagaag caggaggaga aggagatcct gaagtctcag
     2761 ctggacagcc tgctgggcct gtaccacctg ctggactggt ttgccgtgga tgagtccaac
     2821 gaggtggacc ccgagttctc tgcccggctg accggcatca agctggagat ggagccttct
     2881 ctgagcttct acaacaaggc cagaaattat gccaccaaga agccctactc cgtggagaag
     2941 ttcaagctga actttcagat gcctacactg gcctctggct gggacgtgaa taaggagaag
     3001 aacaatggcg ccatcctgtt tgtgaagaac ggcctgtact atctgggcat catgccaaag
     3061 cagaagggca ggtataaggc cctgagcttc gagcccacag agaaaaccag cgagggcttt
     3121 gataagatgt actatgacta cttccctgat gccgccaaga tgatcccaaa gtgcagcacc
     3181 cagctgaagg ccgtgacagc ccactttcag acccacacaa cccccatcct gctgtccaac
     3241 aatttcatcg agcctctgga gatcacaaag gagatctacg acctgaacaa tcctgagaag
     3301 gagccaaaga agtttcagac agcctacgcc aagaaaaccg gcgaccagaa gggctacaga
     3361 gaggccctgt gcaagtggat cgacttcaca agggattttc tgtccaagta taccaagaca
     3421 acctctatcg atctgtctag cctgcggcca tcctctcagt ataaggacct gggcgagtac
     3481 tatgccgagc tgaatcccct gctgtaccac atcagcttcc agagaatcgc cgagaaggag
     3541 atcatggatg ccgtggagac aggcaagctg tacctgttcc agatctataa caaggacttt
     3601 gccaagggcc accacggcaa gcctaatctg cacacactgt attggaccgg cctgttttct
     3661 ccagagaacc tggccaagac aagcatcaag ctgaatggcc aggccgagct gttctaccgc
     3721 cctaagtcca ggatgaagag gatggcacac cggctgggag agaagatgct gaacaagaag
     3781 ctgaaggatc agaaaacccc aatccccgac accctgtacc aggagctgta cgactatgtg
     3841 aatcacagac tgtcccacga cctgtctgat gaggccaggg ccctgctgcc caacgtgatc
     3901 accaaggagg tgtctcacga gatcatcaag gataggcgct ttaccagcga caagttcttt
     3961 ttccacgtgc ctatcacact gaactatcag gccgccaatt ccccatctaa gttcaaccag
     4021 agggtgaatg cctacctgaa ggagcacccc gagacaccta tcatcggcat cgctcggggc
     4081 gagagaaacc tgatctatat cacagtgatc gactccaccg gcaagatcct ggagcagcgg
     4141 agcctgaaca ccatccagca gtttgattac cagaagaagc tggacaacag ggagaaggag
     4201 agggtggcag caaggcaggc ctggtctgtg gtgggcacaa tcaaggatct gaagcagggc
     4261 tatctgagcc aggtcatcca cgagatcgtg gacctgatga tccactacca ggccgtggtg
     4321 gtgctggaga acctgaattt cggctttaag agcaagagga ccggcatcgc cgagaaggcc
     4381 gtgtaccagc agttcgagaa gatgctgatc gataagctga attgcctggt gctgaaggac
     4441 tatccagcag agaaagtggg aggcgtgctg aacccatacc agctgacaga ccagttcacc
     4501 tcctttgcca agatgggcac ccagtctggc ttcctgtttt acgtgcctgc cccatataca
     4561 tctaagatcg atcccctgac cggcttcgtg gaccccttcg tgtggaaaac catcaagaat
     4621 cacgagagcc gcaagcactt cctggagggc ttcgactttc tgcactacga cgtgaaaacc
     4681 ggcgacttca tcctgcactt taagatgaac agaaatctgt ccttccagag gggcctgccc
     4741 ggctttatgc ctgcatggga tatcgtgttc gagaagaacg agacacagtt tgacgccaag
     4801 ggcacccctt tcatcgccgg caagagaatc gtgccagtga tcgagaatca cagattcacc
     4861 ggcagatacc gggacctgta tcctgccaac gagctgatcg ccctgctgga ggagaagggc
     4921 atcgtgttca gggatggctc caacatcctg ccaaagctgc tggagaatga cgattctcac
     4981 gccatcgaca ccatggtggc cctgatccgc agcgtgctgc agatgcggaa ctccaatgcc
     5041 gccacaggcg aggactatat caacagcccc gtgcgcgatc tgaatggcgt gtgcttcgac
     5101 tcccggtttc agaacccaga gtggcccatg gacgccgatg ccaatggcgc ctaccacatc
     5161 gccctgaagg gccagctgct gctgaatcac ctgaaggaga gcaaggatct gaagctgcag
     5221 aacggcatct ccaatcagga ctggctggcc tacatccagg agctgcgcaa caaaaggccg
     5281 gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg gatcctaccc atacgatgtt
     5341 ccagattacg cttatcccta cgacgtgcct gattatgcat acccatatga tgtccccgac
     5401 tatgccggca gtggagaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat
     5461 cctggcccag tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     5521 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     5581 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     5641 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     5701 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     5761 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     5821 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     5881 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     5941 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     6001 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     6061 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     6121 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     6181 aaggaattct aactagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
     6241 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     6301 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     6361 ggggtggggt ggggcaggac agcaaggggg aggattggga agagaatagc aggcatgctg
     6421 gggagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     6481 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     6541 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
     6601 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     6661 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6721 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     6781 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     6841 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     6901 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     6961 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
     7021 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     7081 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     7141 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     7201 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     7261 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     7321 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     7381 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7441 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7501 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7561 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     7621 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     7681 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     7741 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     7801 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     7861 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     7921 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     7981 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8041 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8101 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8161 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggaagcc gcggtatcat
     8221 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8281 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8341 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8401 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8461 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8521 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8581 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8641 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     8701 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8761 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8821 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8881 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8941 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9001 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9061 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9121 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt gagggcctat
     9181 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa
     9241 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
     9301 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
     9361 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
     9421 taatttctac tcttgtagat atctgtcccc tccaccccac agtttttttg ttttagagct
     9481 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     9541 ggtgcttttt tgttttagag ctagaaatag caagttaaaa taaggctagt ccgtttttag
     9601 cgcgtgcgcc aattctgcag acaaatggct ctaggcatgc cgacggcgag gatctcgtcg
     9661 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     9721 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     9781 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     9841 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     9901 gggatccgct gtaagtctgc agaaattgat gatctattaa acaataaaga tgtccactaa
     9961 aatggaagtt tttcctgtca tactttgtta agaagggtga gaacagagta cctacatttt
    10021 gaatggaagg attggagcta cgggggtggg ggtggggtgg gattagataa atgcctgctc
    10081 tttactgaag gctctttact attgctttat gataatgttt catagttgga tatcataatt
    10141 taaacaagca aaaccaaatt aagggccagc tcattcctcc cactcatgat ctatagatct
    10201 atagatctct cgtgggatca ttgtttttct cttgattccc actttgtggt tctaagtact
    10261 gtggtttcca aatgtgtcag tttcatagcc tgaagaacga gatcagcagc ctctgttcca
    10321 catacacttc attctcagta ttgttttgcc aagttctaat tccatcagaa gctgggagct
    10381 ccaccgcggt ggcggccgct ctagaactag ttattaatag taatcaatta cggggtcatt
    10441 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
    10501 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
    10561 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
    10621 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
    10681 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
    10741 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac
    10801 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
    10861 ttgtgcagcg atgggggcgg gggggggggg gggggsgncg ssrggsgggg gggggggggg
    10921 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg
    10981 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg
    11041 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg
    11101 gggggggggg gggggggggg grgggggggg gggggggggg gggkgggtgg gggggggggt
    11161 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
    11221 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
    11281 ccgtgccggg cggg
//
