LOCUS       sequence-381294-       10882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 
            159281), with guide RNA that target tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10882)
  AUTHORS   Haiting Ma, Rudolf Jaenisch
  TITLE     Multiplex genome editing of human pluripotent stem cells using Cpf1
  JOURNAL   bioRxiv
REFERENCE   2  (bases 1 to 10882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv"
COMMENT     Sequence Label: CAGGS-AsCpf1-2A-GFP-U6-tdTomato-sgRNA
FEATURES             Location/Qualifiers
     source          1..10882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     110..129
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     enhancer        866..1169
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1171..1448
                     /label=chicken beta-actin promoter
     intron          1449..2465
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2389..2411
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2473..2492
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2532..2552
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2577..6494
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      6214..6223
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6495..6542
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6549..6629
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             6639..6692
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6693..6695,6696..7406)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(6693..6695,6696..7406)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6735..6756)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6996..7015)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7343..7364
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7434..7451)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7440..7647
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7656..7796
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7656..7785
                     /label=AAV2 ITR
     rep_origin      7871..8326
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7958..7977)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8168..8189
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8343..8362)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8462..8484
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8522..8540)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8608..8712
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8713..8781,8782..9573)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8931..8950)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9744..10332
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10233..10252
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10394..10642
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     10394..10414
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     10565..10584
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        10693..10768
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
       61 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
      121 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
      181 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
      241 ggggatccgc tgtaagtctg cagaaattga tgatctatta aacaataaag atgtccacta
      301 aaatggaagt ttttcctgtc atactttgtt amkawkrrws arwamagakk tccamtaaaa
      361 tggaagtttt tcctgtcata ctttgttaag aagggtgaga acagagtacc tacattttga
      421 atggaaggat tggagctacg ggggtggggg tggggtggga ttagataaat gcctgctctt
      481 tactgaaggc tctttactat tgctttatga taatgtttca tagttggata tcataattta
      541 aacaagcaaa accaaattaa gggccagctc attcctccca ctcatgatct atagatctat
      601 agatctctcg tgggatcatt gtttttctct tgattcccac tttgtggttc taagtactgt
      661 ggtttccaaa tgtgtcagtt tcatagcctg aagaacgaga tcagcagcct ctgttccaca
      721 tacacttcat tctcagtatt gttttgccaa gttctaattc catcagaagc tgggagctcc
      781 accgcggtgg cggccgctct agaactagtt attaatagta atcaattacg gggtcattag
      841 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      901 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      961 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     1021 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     1081 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1141 tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc
     1201 tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt
     1261 gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc rggsggggsg gggsggggsg
     1321 rggggsgggg sggggsgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc
     1381 gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc
     1441 ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc
     1501 gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc
     1561 cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc
     1621 tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg
     1681 gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct
     1741 gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg
     1801 cggccggggg cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc
     1861 ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc
     1921 ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta
     1981 cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg
     2041 gcggggcggg gccgcctcgg gccggggrgg gctcggggga ggggcgcggc ggcccccgga
     2101 gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg
     2161 agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc
     2221 gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg
     2281 gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg
     2341 gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct
     2401 ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc
     2461 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaccggt
     2521 ccaccatggc cccaaagaag aagcggaagg tcggtatcca cggagtccca gcagccacac
     2581 agttcgaggg ctttaccaac ctgtatcagg tgagcaagac actgcggttt gagctgatcc
     2641 cacagggcaa gaccctgaag cacatccagg agcagggctt catcgaggag gacaaggccc
     2701 gcaatgatca ctacaaggag ctgaagccca tcatcgatcg gatctacaag acctatgccg
     2761 accagtgcct gcagctggtg cagctggatt gggagaacct gagcgccgcc atcgactcct
     2821 atagaaagga gaaaaccgag gagacaagga acgccctgat cgaggagcag gccacatatc
     2881 gcaatgccat ccacgactac ttcatcggcc ggacagacaa cctgaccgat gccatcaata
     2941 agagacacgc cgagatctac aagggcctgt tcaaggccga gctgtttaat ggcaaggtgc
     3001 tgaagcagct gggcaccgtg accacaaccg agcacgagaa cgccctgctg cggagcttcg
     3061 acaagtttac aacctacttc tccggctttt atgagaacag gaagaacgtg ttcagcgccg
     3121 aggatatcag cacagccatc ccacaccgca tcgtgcagga caacttcccc aagtttaagg
     3181 agaattgtca catcttcaca cgcctgatca ccgccgtgcc cagcctgcgg gagcactttg
     3241 agaacgtgaa gaaggccatc ggcatcttcg tgagcacctc catcgaggag gtgttttcct
     3301 tcccttttta taaccagctg ctgacacaga cccagatcga cctgtataac cagctgctgg
     3361 gaggaatctc tcgggaggca ggcaccgaga agatcaaggg cctgaacgag gtgctgaatc
     3421 tggccatcca gaagaatgat gagacagccc acatcatcgc ctccctgcca cacagattca
     3481 tccccctgtt taagcagatc ctgtccgata ggaacaccct gtctttcatc ctggaggagt
     3541 ttaagagcga cgaggaagtg atccagtcct tctgcaagta caagacactg ctgagaaacg
     3601 agaacgtgct ggagacagcc gaggccctgt ttaacgagct gaacagcatc gacctgacac
     3661 acatcttcat cagccacaag aagctggaga caatcagcag cgccctgtgc gaccactggg
     3721 atacactgag gaatgccctg tatgagcgga gaatctccga gctgacaggc aagatcacca
     3781 agtctgccaa ggagaaggtg cagcgcagcc tgaagcacga ggatatcaac ctgcaggaga
     3841 tcatctctgc cgcaggcaag gagctgagcg aggccttcaa gcagaaaacc agcgagatcc
     3901 tgtcccacgc acacgccgcc ctggatcagc cactgcctac aaccctgaag aagcaggagg
     3961 agaaggagat cctgaagtct cagctggaca gcctgctggg cctgtaccac ctgctggact
     4021 ggtttgccgt ggatgagtcc aacgaggtgg accccgagtt ctctgcccgg ctgaccggca
     4081 tcaagctgga gatggagcct tctctgagct tctacaacaa ggccagaaat tatgccacca
     4141 agaagcccta ctccgtggag aagttcaagc tgaactttca gatgcctaca ctggcctctg
     4201 gctgggacgt gaataaggag aagaacaatg gcgccatcct gtttgtgaag aacggcctgt
     4261 actatctggg catcatgcca aagcagaagg gcaggtataa ggccctgagc ttcgagccca
     4321 cagagaaaac cagcgagggc tttgataaga tgtactatga ctacttccct gatgccgcca
     4381 agatgatccc aaagtgcagc acccagctga aggccgtgac agcccacttt cagacccaca
     4441 caacccccat cctgctgtcc aacaatttca tcgagcctct ggagatcaca aaggagatct
     4501 acgacctgaa caatcctgag aaggagccaa agaagtttca gacagcctac gccaagaaaa
     4561 ccggcgacca gaagggctac agagaggccc tgtgcaagtg gatcgacttc acaagggatt
     4621 ttctgtccaa gtataccaag acaacctcta tcgatctgtc tagcctgcgg ccatcctctc
     4681 agtataagga cctgggcgag tactatgccg agctgaatcc cctgctgtac cacatcagct
     4741 tccagagaat cgccgagaag gagatcatgg atgccgtgga gacaggcaag ctgtacctgt
     4801 tccagatcta taacaaggac tttgccaagg gccaccacgg caagcctaat ctgcacacac
     4861 tgtattggac cggcctgttt tctccagaga acctggccaa gacaagcatc aagctgaatg
     4921 gccaggccga gctgttctac cgccctaagt ccaggatgaa gaggatggca caccggctgg
     4981 gagagaagat gctgaacaag aagctgaagg atcagaaaac cccaatcccc gacaccctgt
     5041 accaggagct gtacgactat gtgaatcaca gactgtccca cgacctgtct gatgaggcca
     5101 gggccctgct gcccaacgtg atcaccaagg aggtgtctca cgagatcatc aaggataggc
     5161 gctttaccag cgacaagttc tttttccacg tgcctatcac actgaactat caggccgcca
     5221 attccccatc taagttcaac cagagggtga atgcctacct gaaggagcac cccgagacac
     5281 ctatcatcgg catcgatcgg ggcgagagaa acctgatcta tatcacagtg atcgactcca
     5341 ccggcaagat cctggagcag cggagcctga acaccatcca gcagtttgat taccagaaga
     5401 agctggacaa cagggagaag gagagggtgg cagcaaggca ggcctggtct gtggtgggca
     5461 caatcaagga tctgaagcag ggctatctga gccaggtcat ccacgagatc gtggacctga
     5521 tgatccacta ccaggccgtg gtggtgctgg agaacctgaa tttcggcttt aagagcaaga
     5581 ggaccggcat cgccgagaag gccgtgtacc agcagttcga gaagatgctg atcgataagc
     5641 tgaattgcct ggtgctgaag gactatccag cagagaaagt gggaggcgtg ctgaacccat
     5701 accagctgac agaccagttc acctcctttg ccaagatggg cacccagtct ggcttcctgt
     5761 tttacgtgcc tgccccatat acatctaaga tcgatcccct gaccggcttc gtggacccct
     5821 tcgtgtggaa aaccatcaag aatcacgaga gccgcaagca cttcctggag ggcttcgact
     5881 ttctgcacta cgacgtgaaa accggcgact tcatcctgca ctttaagatg aacagaaatc
     5941 tgtccttcca gaggggcctg cccggcttta tgcctgcatg ggatatcgtg ttcgagaaga
     6001 acgagacaca gtttgacgcc aagggcaccc ctttcatcgc cggcaagaga atcgtgccag
     6061 tgatcgagaa tcacagattc accggcagat accgggacct gtatcctgcc aacgagctga
     6121 tcgccctgct ggaggagaag ggcatcgtgt tcagggatgg ctccaacatc ctgccaaagc
     6181 tgctggagaa tgacgattct cacgccatcg acaccatggt ggccctgatc cgcagcgtgc
     6241 tgcagatgcg gaactccaat gccgccacag gcgaggacta tatcaacagc cccgtgcgcg
     6301 atctgaatgg cgtgtgcttc gactcccggt ttcagaaccc agagtggccc atggacgccg
     6361 atgccaatgg cgcctaccac atcgccctga agggccagct gctgctgaat cacctgaagg
     6421 agagcaagga tctgaagctg cagaacggca tctccaatca ggactggctg gcctacatcc
     6481 aggagctgcg caacaaaagg ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa
     6541 agggatccta cccatacgat gttccagatt acgcttatcc ctacgacgtg cctgattatg
     6601 catacccata tgatgtcccc gactatgccg gcagtggaga gggcagagga agtctgctaa
     6661 catgcggtga cgtcgaggag aatcctggcc cagtgagcaa gggcgaggag ctgttcaccg
     6721 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     6781 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     6841 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     6901 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     6961 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     7021 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     7081 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     7141 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     7201 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     7261 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     7321 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     7381 ctctcggcat ggacgagctg tacaaggaat tctaactaga gctcgctgat cagcctcgac
     7441 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     7501 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     7561 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     7621 ggaagagaat agcaggcatg ctggggagcg gccgcaggaa cccctagtga tggagttggc
     7681 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
     7741 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
     7801 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
     7861 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     7921 agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc
     7981 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     8041 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     8101 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     8161 tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc gggctattct
     8221 tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa
     8281 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
     8341 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     8401 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     8461 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     8521 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     8581 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     8641 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     8701 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     8761 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     8821 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     8881 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     8941 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     9001 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     9061 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     9121 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     9181 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     9241 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     9301 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     9361 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     9421 gagcgtggaa gccgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     9481 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     9541 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     9601 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     9661 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     9721 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     9781 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     9841 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     9901 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     9961 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    10021 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    10081 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    10141 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    10201 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    10261 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    10321 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    10381 tttgctcaca tgtgagggcc tatttcccat gattccttca tatttgcata tacgatacaa
    10441 ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa
    10501 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
    10561 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
    10621 cttgtggaaa ggacgaaaca ccgtaatttc tactcttgta gatatgacct cctcgccctt
    10681 gctcactttt ttgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
    10741 acttgaaaaa gtggcaccga gtcggtgctt ttttgtttta gagctagaaa tagcaagtta
    10801 aaataaggct agtccgtttt tagcgcgtgc gccaattctg cagacaaatg gctctaggca
    10861 tgccgacggc gaggatctcg tc
//
