LOCUS       sequence-38137-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3330 (=HL 770 + pHL 1009)
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    34..52
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    59..77
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(98..116)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        184..288
                     /gene="bla"
                     /label=AmpR promoter
     CDS             473..851
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(507..526)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttacgcattt tttttcctcc ttagtcgaca gtatctctat cactgatagg gatgtcaatc
       61 tctatcactg atagggactc gaggtgaaga cgaaagggcc tcgtgatacg cctattttta
      121 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
      181 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      241 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      301 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      361 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      421 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      481 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      541 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      601 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      661 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
      721 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
      781 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
      841 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagc
//
