LOCUS       sequence-38149-e        3114 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3114)
  TITLE     Patrick Moore plasmids
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA6.MCV.LT206.V5(CM2B4)
FEATURES             Location/Qualifiers
     source          1..3114
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2953..2994
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3004..3021
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3044..3061)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 cggatccact agtccagtgt ggtggaattc tgcagatatc atggatttag tcctaaatag
       61 gaaagaaaga gaggctctct gcaagctttt agagattgct cctaattgtt atggcaacat
      121 ccctctgatg aaagctgctt tcaaaagaag ctgcttaaag catcaccctg ataaaggggg
      181 aaatcctgtt ataatgatgg aattgaacac cctttggagc aaattccagc aaaatatcca
      241 caagctcaga agtgacttct ctatgtttga tgaggtcagt acaaaatttc cttgggaaga
      301 atatggaact ttaaaggatt atatgcaaag tggatataat gctagatttt gcagaggtcc
      361 tgggtgcatg cttaagcaac ttagagattc taagtgcgct tgtattagct gtaagttgtc
      421 tcgccagcat tgtagtctaa aaactttaaa gcaaaaaaac tgtctgacgt ggggagagtg
      481 tttttgctat cagtgcttta ttctttggtt tggatttcct cctacttggg aaagttttga
      541 ctggtggcaa aaaactttag aagaaactga ctactgctta ctgcatctgc accttttcta
      601 gactcctact tccttcctct gtaagtatta gatatggaaa agtctataag gcaaaatatc
      661 aaagaaaggt tatttatgac agattttctg tactttccca tctaggttga cgaggcccct
      721 atatatggga ccactaaatt caaagaatgg tggagatcag gaggattcag cttcgggaag
      781 gcatatgaat atgggcccaa tccacacggg accaactcaa gatccagaaa gccttcctcc
      841 aatgcatcca ggggagcccc cagtggaagc tcaccacccc acagccagag ctcttcctct
      901 gggtatgggt ccttctcagc gtcccaggct tcagaccccc agtccagagg acccgatata
      961 cctcccgaac accatgagga acccacctca tcctctggat ccagtagcag agaggagacc
     1021 accaattcag gaagagaatc cagcacaccc aatggaacca gtgtacctag aaattcttcc
     1081 agaacggatg gcacctggga ggatctcttc tgcgatgaat cactttcctc ccctgagcct
     1141 ccctcgtcct ctgaggagcc tgaggagccc ccctcctcaa gaagctcgcc ccggcagccc
     1201 ccgtcttcct ctgccgagga ggcctcgtca tctcagttta cagatgagga atacagatcc
     1261 tcctccttca ccaccccgaa gacccctcct ccattctcaa gaaagcgaaa atttgggggg
     1321 tcccgaagct ctgcaagctc tgctagttca gcaagtttta caagcactcc accaaagcca
     1381 aaaaagaaca gagaaactcc tgttcctact gattttccta ttgatctttc tgattatctt
     1441 agccatgctg tatatagtaa taaaacagta agttgttttg ccatttatac tacttctgat
     1501 aaagctatag agttatatga taagattgag aaatttaaag ttgattttaa aagcaggcat
     1561 gcctgtgaat taggatgtat tttattgttt ataactttat caaagcatag agtatctgct
     1621 attaagaatt tttgctctac cttctgcact ataagctttt taatttgtaa aggagtgaat
     1681 aagatgcctg aaatgtataa taatttatgc aagccccctt acaaattact gcaagagaat
     1741 aagccactgc taaattatga atttcaagaa aaagaaaaag aggccagctg taattggaat
     1801 ttagttgctg aatttgcttg tgaatatgag ctagacgacc actttattat cttagcccat
     1861 tatctagact ttgcaaaacc atttccttgc caaaagtgtg aaaacagatc tcgcctcaaa
     1921 cctcacaagg ctcatgaggc tcatcattct aatgctaagc tattttatga atctaaatct
     1981 cagaaaacca tttgccaaca agccgcagac actgttctag ccaaaaggag gttagagatg
     2041 ctggaaatga ccaggacaga aatgctatgt aagaagttta agaagcacct agagagatta
     2101 agagatttag atacaataga tctactgtat tatatgggtg gtgtggcctg gtactgctgc
     2161 ttatttgaag agtttgaaaa gaagctgcag aaaattattc aattattaac agagaatata
     2221 cctaagtata gaaacatttg gtttaaaggg cctattaaca gtggaaaaac aagctttgct
     2281 gcagccttaa tagatttgct agaagggaag gccttgaata taaactgtcc atctgataag
     2341 ctgccttttg aactaggatg tgctttggat aaatttatgg ttgtttttga ggatgtgaaa
     2401 gggcaaaata gcctaaataa agatctgcaa ccagggcaag gaataaataa ccttgataac
     2461 ttaagagatc atctagatgg tgctgtagct gtaagcttag agaagaagca tgtgaataaa
     2521 aagcatcaga tttttcctcc ttgtattgtt actgctaatg attattttat tcccaaaaca
     2581 ttaatagcaa gatttagtta tactttacac ttttccccaa aggcaaatct aagagattcc
     2641 ctggatcaga acatggaaat aagaaaaaga agaattcttc aaagtggaac cactttattg
     2701 ctttgtctta tttggtgctt gcctgataca acctttaagc cttgcttaca agaagaaatt
     2761 aaaaactgga agcaaatttt acagagtgaa atatcatatg gtaaattttg tcaaatgata
     2821 gaaaatgtag aagctggtca ggaccctctg ctcaatattc ttattgagga agagggccct
     2881 gaggaaactg aagaaaccca agattctggt actttttctc aatactcgag tctagagggc
     2941 ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc
     3001 ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct
     3061 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaa
//
